ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1623 good A KOG1642 RNA processing and modification Ribonuclease, T2 family
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1623 562063 563337 425
(425 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL123c [A] KOG1642 Ribonuclease T2 family 451 e-126
At1g26820 [A] KOG1642 Ribonuclease T2 family 67 7e-11
At2g02990 [A] KOG1642 Ribonuclease T2 family 65 3e-10
At1g14220 [A] KOG1642 Ribonuclease T2 family 64 3e-10
At1g14210 [A] KOG1642 Ribonuclease T2 family 60 5e-09
Hs5231228 [A] KOG1642 Ribonuclease T2 family 54 6e-07
CE28601 [A] KOG1642 Ribonuclease T2 family 53 8e-07
At2g39780 [A] KOG1642 Ribonuclease T2 family 50 5e-06
>YPL123c [A] KOG1642 Ribonuclease T2 family
Length = 434
Score = 451 bits (1160), Expect = e-126
Identities = 215/409 (52%), Positives = 282/409 (68%), Gaps = 16/409 (3%)
Query: 31 SCPISLPASCSNNTEIKDSCCFEFPGGIMLQTQFWDYIPPKGVSDPDELVRHLGPLDSFT 90
+CPI++P SCSN T+I +SCCFE+PGGI LQTQFW+Y P K + ELV+ LGPLDSFT
Sbjct: 26 NCPINIPLSCSNKTDIDNSCCFEYPGGIFLQTQFWNYFPSKNDLNETELVKELGPLDSFT 85
Query: 91 NHGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRD-DLPINGTDLLETMDMYWKSN 149
HGLWPDNC G Y QFCNR IDDV++LL+D +FN D L I+G LLE +D+YWKSN
Sbjct: 86 IHGLWPDNCHGGYQQFCNRSLQIDDVYYLLHDKKFNNNDTSLQISGEKLLEYLDLYWKSN 145
Query: 150 TGDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKLFHDKDTYQT 209
G+ ESLW+HE+NKHGTCI T+ P+CY +WG +K+AVYDYFRI LF DT+ T
Sbjct: 146 NGNHESLWIHEFNKHGTCISTIRPECYTEWGANSVDRKRAVYDYFRITYNLFKKLDTFST 205
Query: 210 LKSAGIEPSVEKSYTKLEISNALKEGHSGEVVHFLCDRHGSLNQIWYFHSLKGSLLGEKF 269
L+ I PSV+ SY+ +I AL + G+ V CDRH SLN++WY++ LKGSLL E F
Sbjct: 206 LEKNNIVPSVDNSYSLEQIEAALSKEFEGKKVFIGCDRHNSLNEVWYYNHLKGSLLSEMF 265
Query: 270 VPISALPKGSNCPDDNIKWYPKGHVPSSYRPPNGNHPGTRGVVRIGN------GKGFLIK 323
VP+ +L +NC D IK++PKG+VP+ R PN RGVVR+ N +GFLIK
Sbjct: 266 VPMDSLAIRTNCKKDGIKFFPKGYVPTFRRRPN-KGARYRGVVRLSNINNGDQMQGFLIK 324
Query: 324 NGHWYLKGTPANFFLIEAPFGNYYLKTRMGYCGI--DNSNKVLACNKNVAQAAQFEYDAK 381
NGHW +GTPAN+ LI++P+GNYYL+T G+C I +SN+++ +N+ A QF++D
Sbjct: 325 NGHWMSQGTPANYELIKSPYGNYYLRTNQGFCDIISSSSNELVCKFRNIKDAGQFDFDPT 384
Query: 382 K---GYIGYNGAYDWYATKYPRGNQQAPVYA---GSNDDGYNFQLKFVK 424
K GYIGY+G Y+W YPR Q+P+++ N Y F+LKF+K
Sbjct: 385 KGGDGYIGYSGNYNWGGDTYPRRRNQSPIFSVDDEQNSKKYKFKLKFIK 433
>At1g26820 [A] KOG1642 Ribonuclease T2 family
Length = 222
Score = 66.6 bits (161), Expect = 7e-11
Identities = 53/201 (26%), Positives = 78/201 (38%), Gaps = 46/201 (22%)
Query: 85 PLDSFTNHGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRDDLPINGTDLLETMDM 144
P F HGLWP+ G + Q CN +S DD + +DL+ +
Sbjct: 50 PAADFGIHGLWPNYKTGGWPQNCNPDSRFDD-----------------LRVSDLMSDLQR 92
Query: 145 YWKS---NTGDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKLF 201
W + + D W HE+ KHGTC + + +DYF +KL
Sbjct: 93 EWPTLSCPSNDGMKFWTHEWEKHGTCAES----------------ELDQHDYFEAGLKLK 136
Query: 202 HDKDTYQTLKSAGIEPSVEKSYTKLEISNALKEGHSGEVVHFL----CDRHGSLNQIWYF 257
+ L +AGI+P +K Y +I N +K +VV F C++ S N Y
Sbjct: 137 QKANLLHALTNAGIKPD-DKFYEMKDIENTIK-----QVVGFAPGIECNKDSSHNSQLYQ 190
Query: 258 HSLKGSLLGEKFVPISALPKG 278
L KF+ +P G
Sbjct: 191 IYLCVDTSASKFINCPVMPHG 211
>At2g02990 [A] KOG1642 Ribonuclease T2 family
Length = 230
Score = 64.7 bits (156), Expect = 3e-10
Identities = 54/197 (27%), Positives = 75/197 (37%), Gaps = 38/197 (19%)
Query: 85 PLDSFTNHGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRDDLPINGTDLLETMDM 144
P F HGLWP+ DGTY C+ D +DLL +M
Sbjct: 58 PAADFGIHGLWPNYKDGTYPSNCDASKPFDS-----------------STISDLLTSMKK 100
Query: 145 YWKS---NTGDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKLF 201
W + +G E+ W HE+ KHGTC ++ ++YF+ A+ L
Sbjct: 101 SWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQ----------------HEYFQTALNLK 144
Query: 202 HDKDTYQTLKSAGIEPSVEKSYTKLEISNALKEGHSGEVVHFLCDRHGSLNQIWYFHSLK 261
+ L AGI P KSY+ I +++KE G C+R GS N Y L
Sbjct: 145 QKTNLLGALTKAGINPD-GKSYSLESIRDSIKES-IGFTPWVECNRDGSGNSQLYQVYLC 202
Query: 262 GSLLGEKFVPISALPKG 278
G + P G
Sbjct: 203 VDRSGSGLIECPVFPHG 219
>At1g14220 [A] KOG1642 Ribonuclease T2 family
Length = 228
Score = 64.3 bits (155), Expect = 3e-10
Identities = 54/205 (26%), Positives = 90/205 (43%), Gaps = 47/205 (22%)
Query: 85 PLDSFTNHGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRDDLPINGTDLLETMDM 144
P F HGLWP+ G++ C+ +S D R + +DL+ ++
Sbjct: 56 PAADFGIHGLWPNYNGGSWPSNCDPDSQFD-------------RSQI----SDLVSSLKK 98
Query: 145 YWKS---NTGDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKLF 201
W + + + + W HE+ KHGTC ++ ++YF A+KL
Sbjct: 99 NWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQ----------------HEYFENALKLK 142
Query: 202 HDKDTYQTLKSAGIEPSVEKSYTKLEISNALKEGHSGEVVHFLC----DRHGSLNQIWYF 257
+ Q LK++GI P + Y +I+NA+K+G G C +R+ L+QI+
Sbjct: 143 QKANLLQILKNSGINPD-DGFYNLDKITNAIKDG-IGFTPGIECNKDPERNAQLHQIYIC 200
Query: 258 HSLKGSLLGEKFVPISALPKGSNCP 282
G+ +F+ LP+GS CP
Sbjct: 201 VDTSGT----EFIECPVLPRGS-CP 220
>At1g14210 [A] KOG1642 Ribonuclease T2 family
Length = 247
Score = 60.5 bits (145), Expect = 5e-09
Identities = 41/150 (27%), Positives = 69/150 (45%), Gaps = 39/150 (26%)
Query: 89 FTNHGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRDDLPINGTDLLETMDMYWK- 147
F HGLWP +GT+ FC+ ++N+ D+ + +DL+ M+ W
Sbjct: 58 FIIHGLWPQFNNGTWPAFCD-QTNLFDISKI----------------SDLVCQMEKKWTE 100
Query: 148 ----SNTGDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKLFHD 203
+ ++ +LW HE+NKHGTC+++++ + YFR +K H
Sbjct: 101 WGVWACPSNETNLWEHEWNKHGTCVQSIFDQ----------------HSYFRTNLKFKHK 144
Query: 204 KDTYQTLKSAGIEPSVEKSYTKLEISNALK 233
L GI+P+ + Y+ EI NA+K
Sbjct: 145 VHLLNILIQKGIKPN-DGFYSLDEIKNAIK 173
>Hs5231228 [A] KOG1642 Ribonuclease T2 family
Length = 256
Score = 53.5 bits (127), Expect = 6e-07
Identities = 41/140 (29%), Positives = 60/140 (42%), Gaps = 42/140 (30%)
Query: 85 PLDSFTNHGLWPDNCDGTYAQFCNRE--SNIDDVWHLLNDDQFNGRDDLPINGTDLLETM 142
P D +T HGLWPD +G CNR N++++ DLL M
Sbjct: 58 PPDYWTIHGLWPDKSEG-----CNRSWPFNLEEI-------------------KDLLPEM 93
Query: 143 DMYWKS---NTGDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMK 199
YW + + W HE+ KHGTC + A NS+K+ YF +++
Sbjct: 94 RAYWPDVIHSFPNRSRFWKHEWEKHGTCAAQV---------DALNSQKK----YFGRSLE 140
Query: 200 LFHDKDTYQTLKSAGIEPSV 219
L+ + D L GI+PS+
Sbjct: 141 LYRELDLNSVLLKLGIKPSI 160
>CE28601 [A] KOG1642 Ribonuclease T2 family
Length = 279
Score = 53.1 bits (126), Expect = 8e-07
Identities = 47/162 (29%), Positives = 65/162 (40%), Gaps = 30/162 (18%)
Query: 92 HGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRDDLPINGTDLLETMDMYWKSNTG 151
HGLWP+ +G+Y Q C F+ I ++ ++Y K
Sbjct: 60 HGLWPNFENGSYPQNCRGTPR-----------HFDENLIKSIEDRLVVVWPNLYPKKTI- 107
Query: 152 DDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKLFHDKDTYQTLK 211
+S W HEY+KHGTC ++ ++ YF MK+F D LK
Sbjct: 108 --QSFWKHEYDKHGTCAQS-------------EKLFESELAYFTEVMKVFDSIDVAGGLK 152
Query: 212 SAGIEPSVEKSYTKLEISNALKEGHSGEVVHFLCDRHGSLNQ 253
S G PS EK T ++ NAL SG+ F C R Q
Sbjct: 153 SVG--PS-EKPITSSDLKNALSGVTSGKTFQFHCLRDKKTKQ 191
>At2g39780 [A] KOG1642 Ribonuclease T2 family
Length = 259
Score = 50.4 bits (119), Expect = 5e-06
Identities = 37/143 (25%), Positives = 55/143 (37%), Gaps = 41/143 (28%)
Query: 89 FTNHGLWPDNCDGTYAQFCNRESNIDDVWHLLNDDQFNGRDDLPINGTDLLETMDMYWKS 148
FT HGLWPD DG++ C R F ++ + L++ ++ YW S
Sbjct: 68 FTIHGLWPDYNDGSWPSCCYRSD-------------FKEKEI-----STLMDGLEKYWPS 109
Query: 149 NT--------GDDESLWVHEYNKHGTCIRTLYPDCYKKWGVAGNSKKQAVYDYFRIAMKL 200
+ G S W HE+ KHGTC ++ D Y+YF + L
Sbjct: 110 LSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHD---------------EYNYFLTTLNL 154
Query: 201 FHDKDTYQTLKSAGIEPSVEKSY 223
+ + L AG S + Y
Sbjct: 155 YLKHNVTDVLYQAGYVASNSEKY 177
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.139 0.456
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,128,220
Number of Sequences: 60738
Number of extensions: 1530963
Number of successful extensions: 2872
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2852
Number of HSP's gapped (non-prelim): 9
length of query: 425
length of database: 30,389,216
effective HSP length: 109
effective length of query: 316
effective length of database: 23,768,774
effective search space: 7510932584
effective search space used: 7510932584
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)