ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1696 good K KOG3149 Transcription Transcription initiation factor IIF, auxiliary subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV1696 588275 587712 -188 
         (188 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOR213c [K] KOG3149 Transcription initiation factor IIF auxiliar...    95   5e-20
YPL129w [K] KOG3149 Transcription initiation factor IIF auxiliar...    94   1e-19
SPAC22H12.02 [K] KOG3149 Transcription initiation factor IIF aux...    82   3e-16
At2g18000 [K] KOG3149 Transcription initiation factor IIF auxili...    52   5e-07
At5g45600 [K] KOG3149 Transcription initiation factor IIF auxili...    48   7e-06

>YOR213c [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
          Length = 248

 Score = 95.1 bits (235), Expect = 5e-20
 Identities = 59/180 (32%), Positives = 96/180 (52%), Gaps = 6/180 (3%)

Query: 1   MQLFILDEEGNEQPATLFDYVTYHLHPTFERPLRKLTEPPFTLDEQGWGEFELKIIAKVK 60
           M+L +LD  G E   T+     YHLH +F++P R+L   PF + E GWGEF LKI     
Sbjct: 35  MELLMLDATGKEVEPTILSKCIYHLHSSFKQPKRRLNSLPFFIKETGWGEFNLKIECFFI 94

Query: 61  FCSTTYTFHHELSFDNNAYNVDYEGRFPYHQTKLREELLNSGSVP---IYNEDEISLGQV 117
             +  ++  H+L+F+++AY VDY    P+  + L  EL     +P   +  E+E+SL ++
Sbjct: 95  GNAGKFSIEHDLTFEDDAYAVDYTVDVPHEFSHLNSELSKYFDLPWKVVSPEEEMSL-RI 153

Query: 118 TNLQRAINLISVSDVDTIQKVINTIVTFPPVSEEIAKRRHQEDDIVILLSQIPNQLLNKI 177
            +L   I  +++ D D +  V+  I+  P V   I     +E    + ++Q+P+ LL KI
Sbjct: 154 ADLP-WIKSLALIDEDMMTDVVQMILNDPAVQRAIENHPRRE-QFFMFITQLPDDLLMKI 211


>YPL129w [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
          Length = 244

 Score = 93.6 bits (231), Expect = 1e-19
 Identities = 45/103 (43%), Positives = 64/103 (61%)

Query: 1   MQLFILDEEGNEQPATLFDYVTYHLHPTFERPLRKLTEPPFTLDEQGWGEFELKIIAKVK 60
           +++ +LD+EG E PAT+FD V YHLHPTF  P R  T+PPF ++EQGWG F L I   + 
Sbjct: 34  IEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLL 93

Query: 61  FCSTTYTFHHELSFDNNAYNVDYEGRFPYHQTKLREELLNSGS 103
             +      H+L+F   +Y V++  + P ++  L EEL  SGS
Sbjct: 94  EKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGS 136


>SPAC22H12.02 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
          Length = 241

 Score = 82.4 bits (202), Expect = 3e-16
 Identities = 42/110 (38%), Positives = 61/110 (55%)

Query: 1   MQLFILDEEGNEQPATLFDYVTYHLHPTFERPLRKLTEPPFTLDEQGWGEFELKIIAKVK 60
           ++L  L+ +G E  A+  D VTY LHPTF+ P R + +PPF + EQGWGEFE++II    
Sbjct: 37  IKLVCLNPQGEETDASFVDRVTYKLHPTFQNPTRTIRKPPFQIKEQGWGEFEMEIIIYYA 96

Query: 61  FCSTTYTFHHELSFDNNAYNVDYEGRFPYHQTKLREELLNSGSVPIYNED 110
                + F H L F    Y+ D E      +  L + L  +G VP Y+++
Sbjct: 97  DKGGEHRFLHYLHFQQEHYHEDIELNINATRPGLLKALTATGEVPGYSDE 146


>At2g18000 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
          Length = 202

 Score = 52.0 bits (123), Expect = 5e-07
 Identities = 27/72 (37%), Positives = 34/72 (46%), Gaps = 3/72 (4%)

Query: 11  NEQPATLFDYVTYHLHPTFERPLRKLTEPPFTLDEQGWGEFELKIIA--KVKFCSTTYTF 68
           NE    +   V +HLHP+F  P R +  PPF L E GWGEF++ I        C      
Sbjct: 74  NEDLGVVIKRVIFHLHPSFNNPTRVVDAPPFALSECGWGEFKIDITVFFHTDVCEKKLEL 133

Query: 69  HHELSFD-NNAY 79
            H L  +  NAY
Sbjct: 134 SHVLKLNPENAY 145


>At5g45600 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
          Length = 268

 Score = 48.1 bits (113), Expect = 7e-06
 Identities = 22/64 (34%), Positives = 32/64 (49%), Gaps = 2/64 (3%)

Query: 11  NEQPATLFDYVTYHLHPTFERPLRKLTEPPFTLDEQGWGEFELKIIAKV--KFCSTTYTF 68
           NE  + +   V + LH +F  P R + EPPF + E GWGEFE+ +        C    + 
Sbjct: 78  NEDISVVVKKVVFQLHSSFNSPTRVIEEPPFEVSESGWGEFEIAMTLHFHSDVCDKPLSL 137

Query: 69  HHEL 72
           +H L
Sbjct: 138 YHHL 141


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.136    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,746,882
Number of Sequences: 60738
Number of extensions: 491316
Number of successful extensions: 1162
Number of sequences better than 1.0e-05: 5
Number of HSP's better than  0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1156
Number of HSP's gapped (non-prelim): 5
length of query: 188
length of database: 30,389,216
effective HSP length: 100
effective length of query: 88
effective length of database: 24,315,416
effective search space: 2139756608
effective search space used: 2139756608
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)