ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1701 good K KOG3149 Transcription Transcription initiation factor IIF, auxiliary subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1701 589517 588837 -227
(227 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL129w [K] KOG3149 Transcription initiation factor IIF auxiliar... 300 8e-82
SPAC22H12.02 [K] KOG3149 Transcription initiation factor IIF aux... 161 6e-40
YOR213c [K] KOG3149 Transcription initiation factor IIF auxiliar... 95 9e-20
YNL107w [K] KOG3149 Transcription initiation factor IIF auxiliar... 53 3e-07
SPAC17G8.07 [K] KOG3149 Transcription initiation factor IIF auxi... 53 4e-07
At5g45600 [K] KOG3149 Transcription initiation factor IIF auxili... 52 5e-07
At2g18000 [K] KOG3149 Transcription initiation factor IIF auxili... 52 5e-07
Hs4758720 [K] KOG3149 Transcription initiation factor IIF auxili... 51 1e-06
Hs5729838 [K] KOG3149 Transcription initiation factor IIF auxili... 51 1e-06
HsM5174571 [K] KOG3149 Transcription initiation factor IIF auxil... 50 2e-06
Hs21361272 [K] KOG3149 Transcription initiation factor IIF auxil... 50 2e-06
7297196 [K] KOG3149 Transcription initiation factor IIF auxiliar... 50 3e-06
CE12388 [K] KOG3149 Transcription initiation factor IIF auxiliar... 49 6e-06
>YPL129w [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 244
Score = 300 bits (769), Expect = 8e-82
Identities = 144/227 (63%), Positives = 171/227 (74%), Gaps = 11/227 (4%)
Query: 1 MRQWTIELVLLDEQGNEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLI 60
+RQW+IE+VLLD++G EIPATIFDKV+YHLHPTFANP+RTFT+PPFRIEEQGWGGF L I
Sbjct: 29 VRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDI 88
Query: 61 SAHLLEKGGEKKITHDLHFMKDAYEIDHVIQVPTNKPLLRAELEKSGPVDEVXXXXXXXX 120
S LLEK GE+KI HDL+F++++YE++HVIQ+P NKPLL EL KSG +E
Sbjct: 89 SVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTI- 147
Query: 121 XXXXXXXXXPGDKRKASTVNGDXXXXXXXXXXXXXXXGSVNLEKLAFGLTKLNEDTLVGV 180
G +R + + GSV+LEKLAFGLTKLNED LVGV
Sbjct: 148 ----------GKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGV 197
Query: 181 VQMITDNKTPEMNITNNIEEGEFVIDLYSLPESLLKSLWEYVKKNTE 227
VQM+TDNKTPEMN+TNN+EEGEF+IDLYSLPE LLKSLW+YVKKNTE
Sbjct: 198 VQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE 244
>SPAC22H12.02 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 241
Score = 161 bits (408), Expect = 6e-40
Identities = 90/224 (40%), Positives = 125/224 (55%), Gaps = 18/224 (8%)
Query: 1 MRQWTIELVLLDEQGNEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLI 60
+R+W+I+LV L+ QG E A+ D+V Y LHPTF NP RT +PPF+I+EQGWG FE+ I
Sbjct: 32 VREWSIKLVCLNPQGEETDASFVDRVTYKLHPTFQNPTRTIRKPPFQIKEQGWGEFEMEI 91
Query: 61 SAHLLEKGGEKKITHDLHFMKDAYEIDHVIQVPTNKPLLRAELEKSGPVDEVXXXXXXXX 120
+ +KGGE + H LHF ++ Y D + + +P L L +G V
Sbjct: 92 IIYYADKGGEHRFLHYLHFQQEHYHEDIELNINATRPGLLKALTATGEVPGYSDEGEEAR 151
Query: 121 XXXXXXXXXPGDKRKASTVNGDXXXXXXXXXXXXXXXGSVNLEKLAFGLTKLNEDTLVGV 180
DKRK + G V+++KLA GL KL ED L+ V
Sbjct: 152 K----------DKRKNESEVG--------AGKKKAKAKPVDMDKLAEGLQKLQEDDLLQV 193
Query: 181 VQMITDNKTPEMNITNNIEEGEFVIDLYSLPESLLKSLWEYVKK 224
VQM+ +NKTP+M + N+IE GEF IDLY+LP++LL L+ + K
Sbjct: 194 VQMVNENKTPDMYVRNDIEGGEFHIDLYTLPDNLLLLLYSFCAK 237
>YOR213c [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 248
Score = 94.7 bits (234), Expect = 9e-20
Identities = 44/105 (41%), Positives = 59/105 (55%)
Query: 1 MRQWTIELVLLDEQGNEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLI 60
+R+W +EL++LD G E+ TI K +YHLH +F P R PF I+E GWG F L I
Sbjct: 30 LRRWQMELLMLDATGKEVEPTILSKCIYHLHSSFKQPKRRLNSLPFFIKETGWGEFNLKI 89
Query: 61 SAHLLEKGGEKKITHDLHFMKDAYEIDHVIQVPTNKPLLRAELEK 105
+ G+ I HDL F DAY +D+ + VP L +EL K
Sbjct: 90 ECFFIGNAGKFSIEHDLTFEDDAYAVDYTVDVPHEFSHLNSELSK 134
>YNL107w [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 226
Score = 53.1 bits (126), Expect = 3e-07
Identities = 23/56 (41%), Positives = 33/56 (58%)
Query: 16 NEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAHLLEKGGEK 71
NE + KVV+ LH T+ NP R+ PPF + E GWG F++ I + +E+ EK
Sbjct: 52 NEDISYFIKKVVFKLHDTYPNPVRSIEAPPFELTETGWGEFDINIKVYFVEEANEK 107
>SPAC17G8.07 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 217
Score = 52.8 bits (125), Expect = 4e-07
Identities = 23/60 (38%), Positives = 32/60 (53%)
Query: 25 KVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAHLLEKGGEKKITHDLHFMKDAY 84
KVV+ LH T+ NP RT PPF + E GWG F++++ + EK +T H Y
Sbjct: 59 KVVFKLHDTYNNPTRTIESPPFEVIETGWGEFDIMVRIFFAPEAHEKALTFYHHLKLHPY 118
>At5g45600 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 268
Score = 52.4 bits (124), Expect = 5e-07
Identities = 21/48 (43%), Positives = 30/48 (61%)
Query: 16 NEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAH 63
NE + + KVV+ LH +F +P R EPPF + E GWG FE+ ++ H
Sbjct: 78 NEDISVVVKKVVFQLHSSFNSPTRVIEEPPFEVSESGWGEFEIAMTLH 125
>At2g18000 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 202
Score = 52.4 bits (124), Expect = 5e-07
Identities = 26/77 (33%), Positives = 40/77 (51%), Gaps = 11/77 (14%)
Query: 16 NEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAHLLEKGGEKKITH 75
NE + +V++HLHP+F NP R PPF + E GWG F++ I+ EKK+
Sbjct: 74 NEDLGVVIKRVIFHLHPSFNNPTRVVDAPPFALSECGWGEFKIDITVFFHTDVCEKKL-- 131
Query: 76 DLHFMKDAYEIDHVIQV 92
E+ HV+++
Sbjct: 132 ---------ELSHVLKL 139
>Hs4758720 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 568
Score = 51.2 bits (121), Expect = 1e-06
Identities = 21/53 (39%), Positives = 32/53 (59%)
Query: 24 DKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAHLLEKGGEKKITHD 76
+KVV+HLH +F P R +PP+++EE G+ GF L I + K +K+ D
Sbjct: 49 EKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFD 101
>Hs5729838 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 227
Score = 50.8 bits (120), Expect = 1e-06
Identities = 25/72 (34%), Positives = 39/72 (53%), Gaps = 4/72 (5%)
Query: 3 QWTIELVLLDEQGNEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISA 62
QWT V + NE + K+ + LH ++ NP R T+PP+ I E GWG FE++I
Sbjct: 46 QWT---VYVKPYRNEDMSAYVKKIQFKLHESYGNPLRVVTKPPYEITETGWGEFEIIIKI 102
Query: 63 HLLEKGGEKKIT 74
++ E+ +T
Sbjct: 103 FFIDP-NERPVT 113
>HsM5174571 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 559
Score = 50.4 bits (119), Expect = 2e-06
Identities = 23/56 (41%), Positives = 34/56 (60%), Gaps = 2/56 (3%)
Query: 24 DKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAHLLEKGGEKKI--THDL 77
+KVV+ LH +F P R EPP+++EE G+ GF + I H K +K+ T+DL
Sbjct: 49 EKVVFWLHDSFPKPRRVCKEPPYKVEESGYAGFIMPIEVHFKNKEEPRKVCFTYDL 104
>Hs21361272 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 559
Score = 50.4 bits (119), Expect = 2e-06
Identities = 23/56 (41%), Positives = 34/56 (60%), Gaps = 2/56 (3%)
Query: 24 DKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISAHLLEKGGEKKI--THDL 77
+KVV+ LH +F P R EPP+++EE G+ GF + I H K +K+ T+DL
Sbjct: 49 EKVVFWLHDSFPKPRRVCKEPPYKVEESGYAGFIMPIEVHFKNKEEPRKVCFTYDL 104
>7297196 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 195
Score = 50.1 bits (118), Expect = 3e-06
Identities = 34/108 (31%), Positives = 52/108 (47%), Gaps = 16/108 (14%)
Query: 3 QWTIELVLLDEQGNEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISA 62
QW V L NE + KV + LH ++ANP+R +PP+ I E GWG FE++I
Sbjct: 42 QWK---VYLKPYFNEDMSIYVKKVHFKLHESYANPNRIVVKPPYEITETGWGEFEVIIKI 98
Query: 63 HLLEKGGEKKITHDLHFMKDAYEIDHVIQVPTNKPLLRAELEKSGPVD 110
+ ++ E+ +T Y I + Q P++ EL S +D
Sbjct: 99 YFNDQ-SERPVT--------CYHILKLFQ----SPVVDGELSSSTTMD 133
>CE12388 [K] KOG3149 Transcription initiation factor IIF auxiliary subunit
Length = 211
Score = 48.9 bits (115), Expect = 6e-06
Identities = 30/86 (34%), Positives = 43/86 (49%), Gaps = 6/86 (6%)
Query: 3 QWTIELVLLDEQGNEIPATIFDKVVYHLHPTFANPHRTFTEPPFRIEEQGWGGFELLISA 62
QWT V L E P KV + LH ++A P+R +PP+ + E GWG FE+ I
Sbjct: 38 QWT---VFLKPYLIEDPTKWIRKVQFKLHESYAVPYRVVEKPPYEVTETGWGEFEIQIRI 94
Query: 63 HLLEKGGEKKIT--HDLHFMKDAYEI 86
+ ++ EK IT H L + E+
Sbjct: 95 YFVDP-NEKPITAFHYLRLFQPTIEL 119
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.137 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,916,274
Number of Sequences: 60738
Number of extensions: 512419
Number of successful extensions: 733
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 718
Number of HSP's gapped (non-prelim): 14
length of query: 227
length of database: 30,389,216
effective HSP length: 103
effective length of query: 124
effective length of database: 24,133,202
effective search space: 2992517048
effective search space used: 2992517048
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)