ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1866 good H KOG1324 Coenzyme transport and metabolism Dihydrofolate reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1866 639883 640578 232
(232 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR236w [H] KOG1324 Dihydrofolate reductase 182 3e-46
At4g34570_1 [H] KOG1324 Dihydrofolate reductase 113 3e-25
At2g16370_1 [H] KOG1324 Dihydrofolate reductase 109 3e-24
7300157 [H] KOG1324 Dihydrofolate reductase 92 5e-19
CE05374 [H] KOG1324 Dihydrofolate reductase 88 9e-18
SPCC1223.08c_2 [H] KOG1324 Dihydrofolate reductase 86 3e-17
At2g21550_1 [H] KOG1324 Dihydrofolate reductase 86 4e-17
Hs4503323 [H] KOG1324 Dihydrofolate reductase 79 5e-15
Hs20470832 [H] KOG1324 Dihydrofolate reductase 72 9e-13
ECU08g0080 [H] KOG1324 Dihydrofolate reductase 69 4e-12
ECU01g1450 [H] KOG1324 Dihydrofolate reductase 69 4e-12
ECU01g0170 [H] KOG1324 Dihydrofolate reductase 69 4e-12
>YOR236w [H] KOG1324 Dihydrofolate reductase
Length = 211
Score = 182 bits (462), Expect = 3e-46
Identities = 98/221 (44%), Positives = 139/221 (62%), Gaps = 22/221 (9%)
Query: 10 VVACLMPGYGIGYQGSLPWRLSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIPAKFRP 69
+VACL P GIG++G LPWRL EMKYFRQ+TS T+D + +NA++MGRKTWESIP KFRP
Sbjct: 11 IVACLQPEMGIGFRGGLPWRLPSEMKYFRQVTSLTKDPNKKNALIMGRKTWESIPPKFRP 70
Query: 70 LPNRLNVVVSRNCQLD-INDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQLIANS 128
LPNR+NV++SR+ + D ++D++ +S L+ AI L +N
Sbjct: 71 LPNRMNVIISRSFKDDFVHDKERSIVQS------------------NSLANAIMNLESNF 112
Query: 129 KILGLETIYIIGGGDIYRQCIPMSHNLFITKIVPDLGMPVPPMDTFLDREQIESQFKEQP 188
K LE IY+IGGG++Y Q ++ + ITKI P P MDTFLD +++E F EQ
Sbjct: 113 K-EHLERIYVIGGGEVYSQIFSITDHWLITKINPLDKNATPAMDTFLDAKKLEEVFSEQD 171
Query: 189 HHKLQEIVPKEVSLPQFSADSSSAPTISEKGFTYSFTLWSR 229
+L+E +P +V LP+ D ++ EKG+ + FTL++R
Sbjct: 172 PAQLKEFLPPKVELPE--TDCDQRYSLEEKGYCFEFTLYNR 210
>At4g34570_1 [H] KOG1324 Dihydrofolate reductase
Length = 232
Score = 113 bits (282), Expect = 3e-25
Identities = 71/178 (39%), Positives = 96/178 (53%), Gaps = 24/178 (13%)
Query: 1 MSCKP-SVCCVVACLMPGYGIGYQGSLPWRLSKEMKYFRQLTSATRDSSMRNAVVMGRKT 59
M+ KP S VV GIG G LPW L ++K+F+ LT +T DS+ +NAVVMGRKT
Sbjct: 11 MTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVVMGRKT 70
Query: 60 WESIPAKFRPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSR 119
WESIP K+RPL RLNVV+SR+ DI + + T S +
Sbjct: 71 WESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCS-------------------SIDS 111
Query: 120 AIDQLIANSKILGLETIYIIGGGDIYRQCI--PMSHNLFITKIVP--DLGMPVPPMDT 173
A+D L A L +E +++IGGGDI R+ + P + IT+I D +P +DT
Sbjct: 112 ALDLLAAPPFSLSIEKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDT 169
>At2g16370_1 [H] KOG1324 Dihydrofolate reductase
Length = 234
Score = 109 bits (273), Expect = 3e-24
Identities = 66/168 (39%), Positives = 90/168 (53%), Gaps = 23/168 (13%)
Query: 10 VVACLMPGYGIGYQGSLPWRLSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIPAKFRP 69
VV GIG G LPW L ++K+F+ +T T DSS +NAVVMGRKTWESIP K+RP
Sbjct: 24 VVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSKKNAVVMGRKTWESIPIKYRP 83
Query: 70 LPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQLIANSK 129
L RLNVV++R+ DI + + T S V A+D L A
Sbjct: 84 LSGRLNVVLTRSGGFDIANTENVVTCSSV-------------------DSALDLLAAPPY 124
Query: 130 ILGLETIYIIGGGDIYRQCI--PMSHNLFITKI--VPDLGMPVPPMDT 173
L +E +++IGGGDI R+ + P + +T+I D +P +DT
Sbjct: 125 CLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDT 172
>7300157 [H] KOG1324 Dihydrofolate reductase
Length = 182
Score = 92.4 bits (228), Expect = 5e-19
Identities = 53/154 (34%), Positives = 82/154 (52%), Gaps = 25/154 (16%)
Query: 10 VVACLMPGYGIGYQGSLPWRLSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIPAKFRP 69
++ + +GIG +G LPWR+ E+KYF + T T D + +NAVVMGRKT+ +P RP
Sbjct: 6 LIVAVCENFGIGIRGDLPWRIKSELKYFSRTTKRTSDPTKQNAVVMGRKTYFGVPESKRP 65
Query: 70 LPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQLIANSK 129
LP+RLN+V+S Q ++ K L +L A+ L ++
Sbjct: 66 LPDRLNIVLSTTLQ------ESDLPK--------------GVLLCPNLETAMKILEEQNE 105
Query: 130 ILGLETIYIIGGGDIYRQCI--PMSHNLFITKIV 161
+ E I+I+GG +Y + + P H L+ITKI+
Sbjct: 106 V---ENIWIVGGSGVYEEAMASPRCHRLYITKIM 136
>CE05374 [H] KOG1324 Dihydrofolate reductase
Length = 189
Score = 88.2 bits (217), Expect = 9e-18
Identities = 61/211 (28%), Positives = 91/211 (42%), Gaps = 44/211 (20%)
Query: 19 GIGYQGSLPWRLSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIPAKFRPLPNRLNVVV 78
GIG G LPWR+ K+M+YF +T D S RNAV+MGRK WESIP RPL RLN+V+
Sbjct: 15 GIGKNGVLPWRIKKDMQYFASVTKNVSDQSKRNAVLMGRKCWESIPVTRRPLAGRLNIVL 74
Query: 79 SRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQLIANSKILGLETIYI 138
SR +D+ + L A+ L + +ETI+
Sbjct: 75 SRQLPAQKSDDY---------------------IVVNSLEAAMKLLSEPPFVDSIETIWN 113
Query: 139 IGGGDIYRQCI--PMSHNLFITKIVPDLGMPVPPMDTFLDREQIESQFKEQPHHKLQEIV 196
IGG +IY + + + +T+I + + + K K++++
Sbjct: 114 IGGAEIYDLALRENLVDEIHLTRIFKNF--------------EADVHLKSLDFSKMEKVQ 159
Query: 197 PKEVSLPQFSADSSSAPTISEKGFTYSFTLW 227
EVS S ++ E G + F W
Sbjct: 160 NAEVS-------SENSEIFEENGLKFEFCKW 183
>SPCC1223.08c_2 [H] KOG1324 Dihydrofolate reductase
Length = 228
Score = 86.3 bits (212), Expect = 3e-17
Identities = 64/217 (29%), Positives = 102/217 (46%), Gaps = 34/217 (15%)
Query: 10 VVACLMPGYGIGYQGSLPWRLSKEMKYFRQLTSATRDSS--------MRNAVVMGRKTWE 61
+VA P GIG + S+PW + +EM YF +TS+T S + N V+MGR ++
Sbjct: 4 IVAVSSPNLGIGKKNSMPWHIKQEMAYFANVTSSTESSGQLEEGKSKIMNVVIMGRSCYD 63
Query: 62 SIPAKFRPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAI 121
S+P K RPL +R+N+V++RN + TK LYA D +
Sbjct: 64 SLPKKNRPLKDRINIVITRNSNYNFG-----LTKKE---------KMPENLYAADCIDSA 109
Query: 122 DQLIAN----SKILGLETIYIIGGGDIYRQCI--PMSHNLFITKIVPDLGMPVPPMDTFL 175
L+A + + ++IIGG +Y + P++ NL T+I + P D+F
Sbjct: 110 LDLVAEKYGADSDIQVGKVFIIGGSFLYGSALYHPLTKNLLFTRIHKEY-----PCDSFF 164
Query: 176 DREQIE-SQFKEQPHHKLQEIVPKEVSLPQFSADSSS 211
E E S + + H +L++ V V + A SS+
Sbjct: 165 PFEPAESSDWVRKAHPELEKFVGIPVEEGRLKAASSN 201
>At2g21550_1 [H] KOG1324 Dihydrofolate reductase
Length = 232
Score = 85.9 bits (211), Expect = 4e-17
Identities = 55/175 (31%), Positives = 87/175 (49%), Gaps = 29/175 (16%)
Query: 10 VVACLMPGYGIGYQGSLPWRLSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIPAKFRP 69
VV G+G LPW L E ++F+ +T+ T D + RNA +MGRK+WES P + RP
Sbjct: 26 VVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTRTSDPTKRNATIMGRKSWESTPLEIRP 85
Query: 70 LPNRLNVVVSR-NCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQLIANS 128
LP RLN+V+++ +C DE L + + A++ L
Sbjct: 86 LPGRLNIVLTKSSCHNIAIDEN--------------------VLVSSSMESALELLATEP 125
Query: 129 KILGLETIYIIGGGDIYRQCIPMS--HNLFITKIVPDLGMP----VPPMDTFLDR 177
L +E +++IGGG++ R + S + +T+I D+ +P P +DT L R
Sbjct: 126 YSLSIEKVFVIGGGELLRNYMNASICDAIHLTEI--DISVPCDAFAPRVDTSLYR 178
>Hs4503323 [H] KOG1324 Dihydrofolate reductase
Length = 187
Score = 79.0 bits (193), Expect = 5e-15
Identities = 66/232 (28%), Positives = 107/232 (45%), Gaps = 56/232 (24%)
Query: 6 SVCCVVACLMPGYGIGYQGSLPWR-LSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIP 64
S+ C+VA + GIG G LPW L E +YF+++T+ + +N V+MG+KTW SIP
Sbjct: 4 SLNCIVA-VSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIP 62
Query: 65 AKFRPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQL 124
K RPL R+N+V+SR +L + F LSR++D
Sbjct: 63 EKNRPLKGRINLVLSR--ELKEPPQGAHF-----------------------LSRSLDDA 97
Query: 125 IANSKILGL----ETIYIIGGGDIYRQCI--PMSHNLFITKIVPDLGMPVPPMDTFLDRE 178
+ ++ L + ++I+GG +Y++ + P LF+T+I+ D DTF
Sbjct: 98 LKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDF-----ESDTFFPEI 152
Query: 179 QIESQFKEQPHHKLQEIVPKEVSLPQFSADSSSAPTISEKGFTYSFTLWSRD 230
+E +KL LP++ S EKG Y F ++ ++
Sbjct: 153 DLEK-------YKL---------LPEYPGVLSDVQ--EEKGIKYKFEVYEKN 186
>Hs20470832 [H] KOG1324 Dihydrofolate reductase
Length = 187
Score = 71.6 bits (174), Expect = 9e-13
Identities = 58/187 (31%), Positives = 89/187 (47%), Gaps = 35/187 (18%)
Query: 9 CVVACLMPGYGIGYQGSLPWR-LSKEMKYFRQLTSATRDSSMRNAVVMGRKTWESIPAKF 67
C+VA + GIG G LP L E +YF+++T+ + +N V+MGRKTW SIP K
Sbjct: 7 CIVA-VSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKN 65
Query: 68 RPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLSRAIDQLIAN 127
RPL +R+N+V+SR + E Q A A L A+
Sbjct: 66 RPLKDRINLVLSRELK-----EPPQ----------------GAHFLARSLDDALKLTERP 104
Query: 128 SKILGLETIYIIGGGDIYRQCIPMSH----NLFITKIVPDLGMPVPPMDTFLDREQIESQ 183
++ I+I+GG +Y++ M+H LF+T+I+ D DTF +E +
Sbjct: 105 ELANKVDMIWIVGGSSVYKEA--MNHLGHLKLFVTRIMQDF-----ESDTFFSEIDLE-K 156
Query: 184 FKEQPHH 190
+K P +
Sbjct: 157 YKLLPEY 163
>ECU08g0080 [H] KOG1324 Dihydrofolate reductase
Length = 205
Score = 69.3 bits (168), Expect = 4e-12
Identities = 61/233 (26%), Positives = 102/233 (43%), Gaps = 48/233 (20%)
Query: 10 VVACLMPGYGIGYQGSLPWR--LSKEMKYFRQLTSA---------TRDSSMRNAVVMGRK 58
+V L GIG +LPW L+ +M +FR L+ + S NAVVMGR+
Sbjct: 4 LVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRR 63
Query: 59 TWESIPAKFRPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLS 118
TW+SIP++FRPL NR+NVV+SR + E T F ++
Sbjct: 64 TWDSIPSRFRPLANRINVVLSRGPAR--STENTFFIQT---------------------F 100
Query: 119 RAIDQLIANSKILGLETIYIIGGGDIYRQCIPMS--HNLFITKIVPDLGMPVPPMDTFLD 176
A+D L + ++IGG D+Y + H +F T++ P D F
Sbjct: 101 EALDSL----PLPPSSMTFVIGGRDVYSLALESGRPHLIFATEVFES-----PECDVFFP 151
Query: 177 REQIESQFKEQPHHKLQEIVPKEVSLPQFSADSSSAPTISEKGFTYSFTLWSR 229
S K + ++ + ++ +S +++ T +E G ++ L+++
Sbjct: 152 HIDWASYEKRDITRDVSRLIDRTLASAFYSPETA---TFTENGTSFKMFLYTK 201
>ECU01g1450 [H] KOG1324 Dihydrofolate reductase
Length = 205
Score = 69.3 bits (168), Expect = 4e-12
Identities = 61/233 (26%), Positives = 102/233 (43%), Gaps = 48/233 (20%)
Query: 10 VVACLMPGYGIGYQGSLPWR--LSKEMKYFRQLTSA---------TRDSSMRNAVVMGRK 58
+V L GIG +LPW L+ +M +FR L+ + S NAVVMGR+
Sbjct: 4 LVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRR 63
Query: 59 TWESIPAKFRPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLS 118
TW+SIP++FRPL NR+NVV+SR + E T F ++
Sbjct: 64 TWDSIPSRFRPLANRINVVLSRGPAR--STENTFFIQT---------------------F 100
Query: 119 RAIDQLIANSKILGLETIYIIGGGDIYRQCIPMS--HNLFITKIVPDLGMPVPPMDTFLD 176
A+D L + ++IGG D+Y + H +F T++ P D F
Sbjct: 101 EALDSL----PLPPSSMTFVIGGRDVYSLALESGRPHLIFATEVFES-----PECDVFFP 151
Query: 177 REQIESQFKEQPHHKLQEIVPKEVSLPQFSADSSSAPTISEKGFTYSFTLWSR 229
S K + ++ + ++ +S +++ T +E G ++ L+++
Sbjct: 152 HIDWASYEKRDITRDVSRLIDRTLASAFYSPETA---TFTENGTSFKMFLYTK 201
>ECU01g0170 [H] KOG1324 Dihydrofolate reductase
Length = 205
Score = 69.3 bits (168), Expect = 4e-12
Identities = 61/233 (26%), Positives = 102/233 (43%), Gaps = 48/233 (20%)
Query: 10 VVACLMPGYGIGYQGSLPWR--LSKEMKYFRQLTSA---------TRDSSMRNAVVMGRK 58
+V L GIG +LPW L+ +M +FR L+ + S NAVVMGR+
Sbjct: 4 LVVALASHRGIGNANALPWPRPLAADMAWFRTLSQSIPLISPDRIALAPSASNAVVMGRR 63
Query: 59 TWESIPAKFRPLPNRLNVVVSRNCQLDINDEQTQFTKSRVXXXXXXXXXXXATLYAGDLS 118
TW+SIP++FRPL NR+NVV+SR + E T F ++
Sbjct: 64 TWDSIPSRFRPLANRINVVLSRGPAR--STENTFFIQT---------------------F 100
Query: 119 RAIDQLIANSKILGLETIYIIGGGDIYRQCIPMS--HNLFITKIVPDLGMPVPPMDTFLD 176
A+D L + ++IGG D+Y + H +F T++ P D F
Sbjct: 101 EALDSL----PLPPSSMTFVIGGRDVYSLALESGRPHLIFATEVFES-----PECDVFFP 151
Query: 177 REQIESQFKEQPHHKLQEIVPKEVSLPQFSADSSSAPTISEKGFTYSFTLWSR 229
S K + ++ + ++ +S +++ T +E G ++ L+++
Sbjct: 152 HIDWASYEKRDITRDVSRLIDRTLASAFYSPETA---TFTENGTSFKMFLYTK 201
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,441,241
Number of Sequences: 60738
Number of extensions: 512134
Number of successful extensions: 1313
Number of sequences better than 1.0e-05: 12
Number of HSP's better than 0.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1292
Number of HSP's gapped (non-prelim): 14
length of query: 232
length of database: 30,389,216
effective HSP length: 103
effective length of query: 129
effective length of database: 24,133,202
effective search space: 3113183058
effective search space used: 3113183058
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)