ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2027 good I KOG2420 Lipid transport and metabolism Phosphatidylserine decarboxylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2027 690272 691747 492
(492 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL169c [I] KOG2420 Phosphatidylserine decarboxylase 560 e-159
SPAC25B8.03 [I] KOG2420 Phosphatidylserine decarboxylase 245 1e-64
Hs13489112 [I] KOG2420 Phosphatidylserine decarboxylase 228 2e-59
7301094 [I] KOG2420 Phosphatidylserine decarboxylase 210 3e-54
At4g16700 [I] KOG2420 Phosphatidylserine decarboxylase 170 5e-42
CE00834 [I] KOG2420 Phosphatidylserine decarboxylase 143 7e-34
SPBC16E9.18 [I] KOG2420 Phosphatidylserine decarboxylase 112 1e-24
At5g57190 [I] KOG2419 Phosphatidylserine decarboxylase 51 5e-06
>YNL169c [I] KOG2420 Phosphatidylserine decarboxylase
Length = 500
Score = 560 bits (1443), Expect = e-159
Identities = 292/509 (57%), Positives = 360/509 (70%), Gaps = 50/509 (9%)
Query: 4 ISRNVSLTMSRIPWPQVPQRVSGPYLKNKTKMLLSQRFM---------SSGPIV-----R 49
+++ +L M R+P + R+ L+N+T +L +++F SSG IV
Sbjct: 10 LAQGRTLLMGRMPAVKFSTRMQ---LRNRTAVLWNRKFSTRLFVQQRRSSGEIVDRAKAA 66
Query: 50 PENSKKRMV--KWVVATSFTLIISSYLINQHYQRIKVAESGDVVEGDSQDKVKGKY--RI 105
NS ++ V KWVV TSFT+++ + L+ D E D+ + KG+ +I
Sbjct: 67 AANSGRKQVSMKWVVLTSFTIVLGTILL---------VSRNDSTEEDATEGKKGRRTRKI 117
Query: 106 KIFNNNWLFFCYSTLPLNAISRLWGQVNSLNLPTWVRPWSYKLYATLFHANLDEMDDPDL 165
KIFNNNWLFFCYSTLPLNA+SRLWGQVNSL LP WVRPW Y+LY+ LF NLDEM+DPDL
Sbjct: 118 KIFNNNWLFFCYSTLPLNAMSRLWGQVNSLTLPIWVRPWGYRLYSFLFGVNLDEMEDPDL 177
Query: 166 SHYENLSQFFYRTIRPETRPIDFDENVVVCPSDGTVLQFGIIDPHLGHIEQVKGMTYSVK 225
+HY NLS+FFYR I+P TRP+ E+V+ PSDG +LQ GII+ G IEQVKGMTYS+K
Sbjct: 178 THYANLSEFFYRNIKPGTRPVAQGEDVIASPSDGKILQVGIINSETGEIEQVKGMTYSIK 237
Query: 226 EFLGTHGHPLLQRTPSEEKLGQDTKTDNDHREFARINDIPYSLNDIIGHTPGSQSEDHLQ 285
EFLGTH HPL+ ++ S L D + HREFAR+N I + SED Q
Sbjct: 238 EFLGTHSHPLMSKSASSLDLTSD---EEKHREFARVNRIQLA-----------GSEDTEQ 283
Query: 286 PF-DYKMEKNQAISD--QDSSKTFTLLGELSPNYVKQKLSST---DTQLYFAVIYLAPGD 339
P ++K E +Q++ + SK LL +LS NY S + DT+L+FAVIYLAPGD
Sbjct: 284 PLLNFKNEGDQSVREFKPSVSKNIHLLSQLSLNYFSNGFSCSEPHDTELFFAVIYLAPGD 343
Query: 340 YHHYHSPVNWVCKLRRHFPGELFSVAPYFQRNFPNLFVLNERVALLGHWKHGFFSMTPVG 399
YHH+HSPV+WVCK+RRHFPG+LFSVAPYFQRNFPNLFVLNERVALLG WK+GFFSMTPVG
Sbjct: 344 YHHFHSPVDWVCKVRRHFPGDLFSVAPYFQRNFPNLFVLNERVALLGSWKYGFFSMTPVG 403
Query: 400 ATNVGSIKLNFDKELITNEKTQRHCKPHTCYEATYENASKVLGGVPLIKGEEMGGFMLGS 459
ATNVGSIKLNFD+E +TN K+ +H +PHTCY+A YENASK+LGG+PL+KGEEMGGF LGS
Sbjct: 404 ATNVGSIKLNFDQEFVTNSKSDKHLEPHTCYQAVYENASKILGGMPLVKGEEMGGFELGS 463
Query: 460 TVVLCFEAPREFNFKINVGDKVKMGQPLG 488
TVVLCFEAP EF F + VGDKVKMGQ LG
Sbjct: 464 TVVLCFEAPTEFKFDVRVGDKVKMGQKLG 492
>SPAC25B8.03 [I] KOG2420 Phosphatidylserine decarboxylase
Length = 516
Score = 245 bits (625), Expect = 1e-64
Identities = 163/446 (36%), Positives = 216/446 (47%), Gaps = 67/446 (15%)
Query: 109 NNNWLFFCYSTLPLNAISRLWGQVNSLNLPTWVRPWSYKLYATLFHANLDEMDDPDLSHY 168
N +W + S+LPL ++SR+WGQ N +LPT++R +KLYA +F NL E+ DPDL+HY
Sbjct: 71 NGSWQVYLLSSLPLRSLSRVWGQFNRAHLPTFLRTPGFKLYAWVFGCNLSELKDPDLTHY 130
Query: 169 ENLSQFFYRT----------IRPETRPID--------FDENVV----------------V 194
N FF R + P P+D D N + +
Sbjct: 131 RNFQDFFCRELRPETRPVDPVSPVVSPVDGRIVCQGVVDNNRIQHVKGLSYSLEALLGGI 190
Query: 195 CPSDGTVLQF------GIIDPHLGHIEQVKGMTYSVKEFLGTHGHPLLQRTPSEEKLGQD 248
S+ V+ F +I H EQ S + + S+E+
Sbjct: 191 SSSNPLVVNFEDEITPDLIQKHEQFAEQHSISLNSNNRYRKADASAAVVDEHSDEEALLC 250
Query: 249 TKTD------NDHRE-------FARINDIPYSLNDIIGHTPGSQSEDHLQPFDYKMEKNQ 295
TD ND R F+ DI S+ G S L +
Sbjct: 251 AFTDHPHFYLNDSRNSLNYFCPFSAFEDISNSVRSSCGKRLSPSSNFDLNNLGGDDDLRS 310
Query: 296 AISDQDSSKTFTLLGELSPNYVK--QKLSSTD------------TQLYFAVIYLAPGDYH 341
S S ++L N+ Q+ TD +L+++VIYLAPGDYH
Sbjct: 311 ESSSDFESAPASILEHEPTNWDDWVQEADVTDIDSLPWHNIRPGNKLFYSVIYLAPGDYH 370
Query: 342 HYHSPVNWVCKLRRHFPGELFSVAPYFQRNFPNLFVLNERVALLGHWKHGFFSMTPVGAT 401
+HSP +WV + RRHF GELFSV+P+ R NLFVLNERVALLG ++HGF SM PVGAT
Sbjct: 371 RFHSPADWVIESRRHFSGELFSVSPFLARRLHNLFVLNERVALLGRYEHGFMSMIPVGAT 430
Query: 402 NVGSIKLNFDKELITNEKTQRHCKPHTCYEATYENASKVLGGVPLIKGEEMGGFMLGSTV 461
NVGSI +N D L TN R T EA Y+NAS VL G+P+ +GE++GGF LGSTV
Sbjct: 431 NVGSIVINCDPTLSTNRLVLRKKSLGTFQEAVYKNASPVLDGMPVSRGEQVGGFQLGSTV 490
Query: 462 VLCFEAPREFNFKINVGDKVKMGQPL 487
VL FEAP +F F G V++G+ L
Sbjct: 491 VLVFEAPADFEFSTYQGQYVRVGEAL 516
>Hs13489112 [I] KOG2420 Phosphatidylserine decarboxylase
Length = 375
Score = 228 bits (581), Expect = 2e-59
Identities = 144/378 (38%), Positives = 190/378 (50%), Gaps = 83/378 (21%)
Query: 117 YSTLPLNAISRLWGQVNSLNLPTWVRPWSYKLYATLFHANLDEMDDPDLSHYENLSQFFY 176
Y ++P +SR WG++N + LP W+R Y LY F N+ E DL HY NLS+FF
Sbjct: 77 YKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFR 136
Query: 177 RTIRPETRPIDFDENVVVCPSDGTVLQFGIIDPHLGHIEQVKGMTYSVKEFLGTHGHPLL 236
R ++P+ RP+ + V+ PSDG +L FG QVK
Sbjct: 137 RKLKPQARPV-CGLHSVISPSDGRILNFG----------QVKNC---------------- 169
Query: 237 QRTPSEEKLGQDTKTDNDHREFARINDIPYSLNDIIGHTPGSQSEDHLQPFDYKMEKNQA 296
E ++ + YSL +G P +ED PF
Sbjct: 170 --------------------EVEQVKGVTYSLESFLG--PRMCTED--LPFP-------P 198
Query: 297 ISDQDSSKTFTLLGELSPNYVKQKLSSTDTQLYFAVIYLAPGDYHHYHSPVNWVCKLRRH 356
+ DS K Q ++ +LY VIYLAPGDYH +HSP +W RRH
Sbjct: 199 AASCDSFKN-------------QLVTREGNELYHCVIYLAPGDYHCFHSPTDWTVSHRRH 245
Query: 357 FPGELFSVAPYFQRNFPNLFVLNERVALLGHWKHGFFSMTPVGATNVGSIKLNFDKELIT 416
FPG L SV P R LF NERV L G WKHGFFS+T VGATNVGSI++ FD++L T
Sbjct: 246 FPGSLMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDRDLHT 305
Query: 417 NEKTQRHCKPHTCYEATYENASKVL----GGVPLIKGEEMGGFMLGSTVVLCFEAPREFN 472
N + RH K +Y + S V GVP+ KGE +G F LGST+VL FEAP++FN
Sbjct: 306 N--SPRHSK------GSYNDFSFVTHTNREGVPMRKGEHLGEFNLGSTIVLIFEAPKDFN 357
Query: 473 FKINVGDKVKMGQPLGKV 490
F++ G K++ G+ LG +
Sbjct: 358 FQLKTGQKIRFGEALGSL 375
>7301094 [I] KOG2420 Phosphatidylserine decarboxylase
Length = 447
Score = 210 bits (535), Expect = 3e-54
Identities = 138/378 (36%), Positives = 181/378 (47%), Gaps = 78/378 (20%)
Query: 117 YSTLPLNAISRLWGQVNSLNLPTWVRPWSYKLYATLFHANLDEMDDPDLSHYENLSQFFY 176
Y +LPL ISR WG + + LP +RP+ Y Y+ F NL E P+ HY +L++FF
Sbjct: 140 YCSLPLRIISRCWGWLAACYLPPSLRPYVYGWYSNTFDVNLSEAMYPEYEHYNSLAEFFT 199
Query: 177 RTIRPETRPIDFDENVVVCPSDGTVLQFGIIDPHLGHIEQVKGMTYSVKEFLGTHGHPLL 236
R ++ R ID + +V P+DG VL FG L IEQVKG
Sbjct: 200 RPLKEGVRVID-QQAPLVSPADGKVLHFGSASDSL--IEQVKG----------------- 239
Query: 237 QRTPSEEKLGQDTKTDNDHREFARINDIPYSLNDIIGHTPGSQSEDHLQPFDYKMEKNQA 296
+ YS+ D +G P + + N
Sbjct: 240 ---------------------------VSYSIEDFLG------------PLETVEQANSG 260
Query: 297 ISDQDSSKTFTLLGELSPNYVKQKLSSTDTQLYFAVIYLAPGDYHHYHSPVNWVCKLRRH 356
S + K K S T+LY VIYLAPGDYH +HSP W +RRH
Sbjct: 261 ASYAQALK---------------KKSDGSTELYQCVIYLAPGDYHRFHSPTAWKPTIRRH 305
Query: 357 FPGELFSVAPYFQRNFPNLFVLNERVALLGHWKHGFFSMTPVGATNVGSIKLNFDKELIT 416
F GEL SV+P P LF LNERV +G WKHGFFS T VGATNVGS+++ D +L T
Sbjct: 306 FSGELLSVSPKVAGWLPGLFCLNERVLYMGQWKHGFFSYTAVGATNVGSVEIYMDADLKT 365
Query: 417 NEKTQRHCKPH--TCYEATYENASKVLGGVP--LIKGEEMGGFMLGSTVVLCFEAPREFN 472
N T + H + YE +K L P KG+ +G F +GST+VL FEAP+ F
Sbjct: 366 NRWTGFNVGKHPPSTYEYDELVLNKELTEAPKEFGKGDLVGQFNMGSTIVLLFEAPKNFK 425
Query: 473 FKINVGDKVKMGQPLGKV 490
F I G K+++G+ LG +
Sbjct: 426 FDIIAGQKIRVGESLGHI 443
>At4g16700 [I] KOG2420 Phosphatidylserine decarboxylase
Length = 434
Score = 170 bits (430), Expect = 5e-42
Identities = 127/403 (31%), Positives = 191/403 (46%), Gaps = 69/403 (17%)
Query: 63 ATSFTLIISSYLINQHYQRIKVAESGDVVEGDSQDKVKGKYRIKI-FNNNWLFFCYSTLP 121
AT T+++ L H +R+ + + D+Q + K + I++ F+ + LP
Sbjct: 76 ATMATILMLGAL---HARRLYEDKKHRFLLADTQIEEKREKGIELEFHPDIKASFLGVLP 132
Query: 122 LNAISRLWGQVNSLNLPTWVRPWSYKLYATLFHANLDEMDDPDLSHYENLSQFFYRTIRP 181
L +ISR WG SL +P W+RP++YK +A FH+NL+E P L Y +L FF R+++
Sbjct: 133 LRSISRAWGSFMSLEIPVWMRPYAYKAWARAFHSNLEEAALP-LEEYTSLQDFFVRSLKE 191
Query: 182 ETRPIDFDENVVVC----------PSDGTVLQFGIIDPHLGHIEQVKGMTYSVKEFLGTH 231
RPID D + C P DGTVL+FG + + G IEQVKG +YSV LG
Sbjct: 192 GCRPIDPDPCCLFCSLRFRRYQVSPVDGTVLRFGELKGNRGMIEQVKGHSYSVPALLGN- 250
Query: 232 GHPLLQRTPSEEKLGQDTKTDNDHREFARINDIPYSLNDIIGHTPGSQSEDHLQPFDYKM 291
N ++ P ++E
Sbjct: 251 -------------------------------------NSLLPMEPEGKNE---------- 263
Query: 292 EKNQAISDQDSSKTFTLLGELSPNYVKQKLSSTDTQLYFAVIYLAPGDYHHYHSPVNWVC 351
K +A+ D+ S K++ + SP + +S LY+ VIYL PGDYH HSP +W
Sbjct: 264 SKEEAVGDK-SDKSWLRVSLASPKLRENVSASPMKGLYYCVIYLKPGDYHRIHSPADWNA 322
Query: 352 KLRRHFPGELFSVAPYFQRNFPNL--FVLNERVALLGHWKHGFFSMTPVGATNVGSIKLN 409
+RRHF G ++ PN+ +L ++V L G WK GF ++ VGATN+GSI+L
Sbjct: 323 TVRRHFAGLVYQPNMSGILLSPNIDHQLLIQQVVLEGIWKEGFMALAAVGATNIGSIELF 382
Query: 410 FDKELITNEKTQRHCKPHTCYEATYENASKVLGGVPLIKGEEM 452
+ EL TN+ ++ E Y+ G+ L KG+E+
Sbjct: 383 IEPELRTNKPKKKLFPTEPPEERVYDPEGL---GLRLEKGKEV 422
>CE00834 [I] KOG2420 Phosphatidylserine decarboxylase
Length = 333
Score = 143 bits (360), Expect = 7e-34
Identities = 78/166 (46%), Positives = 94/166 (55%), Gaps = 15/166 (9%)
Query: 325 DTQLYFAVIYLAPGDYHHYHSPVNWVCKLRRHFPGELFSVAPYFQRNFPNLFVLNERVAL 384
+ LY VIYLAPGDYH +HSP WV RH PG L SV P + P+LF LNERV L
Sbjct: 175 ELDLYQVVIYLAPGDYHAFHSPARWVANQCRHVPGLLLSVRPTLLSHVPHLFCLNERVVL 234
Query: 385 LGHWKHGFFSMTPVGATNVGSIKLNFDKELITN---EKTQRHCKPHTCYEATYENASKVL 441
G W+HGFFSM+ V ATNVG I ++ + L TN KTQ+ T A
Sbjct: 235 NGSWRHGFFSMSAVAATNVGDIVVDAEPSLRTNIVRRKTQKIMNTETEIHA--------- 285
Query: 442 GGVPLIKGEEMGGFMLGSTVVLCFEAPREFNFKINVGDKVKMGQPL 487
P + GE +G F LGST+VL F+AP F I GD ++ GQ L
Sbjct: 286 ---PYVSGERVGEFRLGSTIVLVFQAPPTIKFAIKAGDPLRYGQSL 328
Score = 85.5 bits (210), Expect = 2e-16
Identities = 45/124 (36%), Positives = 70/124 (56%), Gaps = 4/124 (3%)
Query: 106 KIFNNNWLFFCYSTLPLNAISRLWGQVNSLNLPTWVRPWSYKLYATLFHANLDEMDDPDL 165
K + +NW Y +LP N SR+ G + + +P W+R +A ++ +D+ DPD
Sbjct: 47 KHYYSNWKIRVYLSLPFNTASRVIGGLANQEIPVWLREHLLGGFARMYDCRMDDCVDPDF 106
Query: 166 SHYENLSQFFYRTIRPETRPIDFDENVVVCPSDGTVLQFGIIDPHLGHIEQVKGMTYSVK 225
+Y + + FF R ++ TRPI + +V P+DGTVL FG ++ + IE VKG Y V
Sbjct: 107 KNYPSFAAFFNRKLKESTRPI--SASPLVSPADGTVLHFGKVEDN--KIEYVKGHDYDVD 162
Query: 226 EFLG 229
+FLG
Sbjct: 163 KFLG 166
>SPBC16E9.18 [I] KOG2420 Phosphatidylserine decarboxylase
Length = 203
Score = 112 bits (280), Expect = 1e-24
Identities = 57/146 (39%), Positives = 89/146 (60%), Gaps = 4/146 (2%)
Query: 94 DSQDKVKGKYRIKIFNNNWLFFCYSTLPLNAISRLWGQVNSLNLPTWVRPWSYKLYATLF 153
D +K K +++ W F+ +TLPL +SR WG VN + +P W+R ++ LY+ +F
Sbjct: 55 DRHEKTYQKKGVQV-EGPWQFYVLTTLPLRTLSRWWGYVNRIEIPLWMRVPAFGLYSKIF 113
Query: 154 HANLDEMDDPDLSHYENLSQFFYRTIRPETRPIDFDENVVVCPSDGTVLQFGIIDPHLGH 213
NL E D D+ Y+NL++FF R ++P R ID D +V+ P+DG +L +G+I+ G
Sbjct: 114 GCNLTEADPDDVRQYKNLAEFFTRKLKPGARVIDPDAPIVI-PADGKILNYGVIEG--GQ 170
Query: 214 IEQVKGMTYSVKEFLGTHGHPLLQRT 239
+EQVKG+TYS+ LG L+R+
Sbjct: 171 LEQVKGITYSLDALLGDEKLARLKRS 196
>At5g57190 [I] KOG2419 Phosphatidylserine decarboxylase
Length = 615
Score = 50.8 bits (120), Expect = 5e-06
Identities = 41/135 (30%), Positives = 60/135 (44%), Gaps = 32/135 (23%)
Query: 332 VIYLAPGDYHHYHSPVNWVCKLRRHFPGELFSVAPY-FQRNFPNLFVLNER-VALLGHWK 389
+ LAP DYH +H PV+ V + G L++V P + N+F N+R VA++ +
Sbjct: 467 IFRLAPQDYHRFHVPVSGVIEQFVDVSGSLYTVNPIAVNSKYCNVFTENKRTVAIISTAE 526
Query: 390 HGFFSMTPVGATNVGSIKLNFDKELITNEKTQRHCKPHTCYEATYENASKVLGGVPLIKG 449
G + +GAT VGSI NF + K H K KG
Sbjct: 527 FGKVAFVAIGATMVGSI--NFVR------KEGEHVK----------------------KG 556
Query: 450 EEMGGFMLGSTVVLC 464
+E+G F G + V+C
Sbjct: 557 DELGYFSFGGSTVIC 571
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,670,046
Number of Sequences: 60738
Number of extensions: 1444076
Number of successful extensions: 3087
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3053
Number of HSP's gapped (non-prelim): 14
length of query: 492
length of database: 30,389,216
effective HSP length: 110
effective length of query: 382
effective length of database: 23,708,036
effective search space: 9056469752
effective search space used: 9056469752
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)