ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV2086   suspect: LH   R     KOG0118  General function prediction only                               FOG: RRM domain

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV2086 712747  713406 220  
         (220 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC365.04c [R] KOG0118 FOG: RRM domain                                80   3e-15
7291851 [R] KOG0110 RNA-binding protein (RRM superfamily)              49   5e-06

>SPBC365.04c [R] KOG0118 FOG: RRM domain
          Length = 233

 Score = 79.7 bits (195), Expect = 3e-15
 Identities = 55/161 (34%), Positives = 80/161 (49%), Gaps = 27/161 (16%)

Query: 69  RFIVFVGNIGRETTASQLQAHFK-ASSPDHIRMRQDK------GIAFLEFDGDKDHKNIQ 121
           + I+FVGN+ ++++   LQ HFK A     +R+  DK      G AF+EF   K      
Sbjct: 91  KVILFVGNLPKDSSVETLQLHFKRAGQVPSVRIPTDKTSGRQKGYAFVEFINPKTDV--- 147

Query: 122 RRMDVALLQHKTMLGDRKINVELTVGGGGNSATRLEKLRTKNDKLDIERNQRMAKLISDS 181
             +  AL  H T+  +RKIN+ELT GGGG +  R+ K++ KN K   E  QR+A      
Sbjct: 148 --ISKALKFHHTIYKERKINIELTAGGGGKTEARMNKIKEKNRKWKEEMRQRVA------ 199

Query: 182 RGNKKTDQDSESNGAEKNEPK--KPSSVPAGIHPDRARFIK 220
                   + +  G EK   K      + +GIHPDR R ++
Sbjct: 200 -------SEEQQAGEEKMARKAVADEGLESGIHPDRLRLLQ 233


>7291851 [R] KOG0110 RNA-binding protein (RRM superfamily)
          Length = 250

 Score = 48.9 bits (115), Expect = 5e-06
 Identities = 42/149 (28%), Positives = 67/149 (44%), Gaps = 19/149 (12%)

Query: 68  NRFIVFVGNIGRETTASQLQAHFKASSPD---HIRMRQDKGIAFLEFDGDKDHKNIQRRM 124
           NR +++V N+  ETT   L+ HF A+       I  ++  G AF+E       +N     
Sbjct: 112 NRHVLYVTNLNFETTKDDLELHFSAAGTVKSIRIPKKRRGGFAFVEMADLSSFQN----- 166

Query: 125 DVALLQHKTMLGDRKINVELTVGGGGNSATRLEKLRTKNDKLDIERNQRMAKLISDSRGN 184
             A   H T L  R I V+++  G   SA +   ++ KN KL   RN++     + ++  
Sbjct: 167 --AFQLHNTELQGRNIKVQISEAGKKKSANKKNIIKQKNRKLAEMRNEQK----TFTKSG 220

Query: 185 KKTDQDSESNGAE-----KNEPKKPSSVP 208
           K  D+D +   A+     K   KKP+  P
Sbjct: 221 KFYDKDLKKEKAKEMLARKRWRKKPAPRP 249


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.131    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,096,571
Number of Sequences: 60738
Number of extensions: 361305
Number of successful extensions: 1552
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1548
Number of HSP's gapped (non-prelim): 2
length of query: 220
length of database: 30,389,216
effective HSP length: 102
effective length of query: 118
effective length of database: 24,193,940
effective search space: 2854884920
effective search space used: 2854884920
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)