ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2125 good E KOG2250 Amino acid transport and metabolism Glutamate/leucine/phenylalanine/valine dehydrogenases
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2125 722139 725234 1032
(1032 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL215c [E] KOG2250 Glutamate/leucine/phenylalanine/valine dehyd... 1288 0.0
SPCC132.04c [E] KOG2250 Glutamate/leucine/phenylalanine/valine d... 652 0.0
At3g03910 [E] KOG2250 Glutamate/leucine/phenylalanine/valine deh... 54 1e-06
At5g18170 [E] KOG2250 Glutamate/leucine/phenylalanine/valine deh... 53 2e-06
>YDL215c [E] KOG2250 Glutamate/leucine/phenylalanine/valine dehydrogenases
Length = 1092
Score = 1288 bits (3333), Expect = 0.0
Identities = 649/1078 (60%), Positives = 812/1078 (75%), Gaps = 64/1078 (5%)
Query: 7 PDISTLSISSIADYYNFEFPGKSAQKEQVVDLLDQQGFIPDTLLENEVDWFYEGLGIDDY 66
PDI++LSISS++DY+ F+FPGK Q+E+V+DLLDQQGFIPD L+E EVDWFY LGIDD
Sbjct: 17 PDIASLSISSMSDYHVFDFPGKDLQREEVIDLLDQQGFIPDDLIEQEVDWFYNSLGIDDL 76
Query: 67 FFSKEVPETIANVISTLYASKVESFAQNH------QKLQVEKAII-NDSHAMFLDTN--- 116
FFS+E P+ I+N+I +LYASK++ FA++ + ++ II ND+HA+F+++N
Sbjct: 77 FFSRESPQLISNIIHSLYASKLDFFAKSKFNGIQPRLFSIKNKIITNDNHAIFMESNTGV 136
Query: 117 ------------------------------------------HHLDTEIDSKFLDSE-EH 133
+ LD+EID FLD++ +
Sbjct: 137 SISDSQQKNFKFASDAVGNDTLEHGKDTIKKNRIEMDDSCPPYELDSEIDDLFLDNKSQK 196
Query: 134 HYRLISFLA-ENSLKLSFVYQNEYPTGNASSIELTDEQLTSGDIDSISDVTMITMTSQEN 192
+ RL+SF A E+ LKL+FVY++ YP + + ++++ + L GDI+SISD TM ++S EN
Sbjct: 197 NCRLVSFWAPESELKLTFVYESVYPNDDPAGVDISSQDLLKGDIESISDKTMYKVSSNEN 256
Query: 193 KKLYGQLIHHVSQREGPVIKTFNSVANANEVRMCIAFKRSSTKGXXXXXXXXXXXXXXKH 252
KKLYG L+ V +REGPVIKT SV N +E+R+ +A+KR +TK K
Sbjct: 257 KKLYGLLLKLVKEREGPVIKTTRSVENKDEIRLLVAYKRFTTKRYYSALNSLFHYYKLKP 316
Query: 253 SKLYVDTFA---NDVSIISIYLDKSQQDHDIL-KELGLSLLQVEREASLLYAIPQNSFKD 308
SK Y+++F +D+ I S+YL+++QQ D+L ++ +L QVEREASLLYAIP NSF +
Sbjct: 317 SKFYLESFNVKDDDIIIFSVYLNENQQLEDVLLHDVEAALKQVEREASLLYAIPNNSFHE 376
Query: 309 LYQSRQFSPQESIYAHIGSIFINHFINRLGSSYDSLIRQLNVKPDDTLLNELLANLKRKL 368
+YQ RQFSP+E+IYAHIG+IFINHF+NRLGS Y +L+ Q+ +K +DT L E++ NLKRKL
Sbjct: 377 VYQRRQFSPKEAIYAHIGAIFINHFVNRLGSDYQNLLSQITIKRNDTTLLEIVENLKRKL 436
Query: 369 RTETYTQQMIIHVLHKYKEIVSKLYKSFAQVHYPTKTSATGSGNQKLQMTLSYQRLSQLE 428
R ET TQQ II+++ K+ I+SKLYK+FAQ+HY ++ + ++ TLS+QRL ++E
Sbjct: 437 RNETLTQQTIINIMSKHYTIISKLYKNFAQIHYYHNST------KDMEKTLSFQRLEKVE 490
Query: 429 PFQSEEEFNLYLNKVFPNDSPDLLILRTLNLFNKSILKTNFFITRKVAISFKLDPALILP 488
PF++++EF YLNK PNDSPDLLIL+TLN+FNKSILKTNFFITRKVAISF+LDP+L++
Sbjct: 491 PFKNDQEFEAYLNKFIPNDSPDLLILKTLNIFNKSILKTNFFITRKVAISFRLDPSLVMT 550
Query: 489 KSEYPETPYGIFFVVGNTFKAFHIRFRDISRGGIRIVVSRSQDVYDKNSKSVIDENYGLA 548
K EYPETPYGIFFVVGNTFK FHIRFRDI+RGGIRIV SR+QD+YD NSK+VIDENY LA
Sbjct: 551 KFEYPETPYGIFFVVGNTFKGFHIRFRDIARGGIRIVCSRNQDIYDLNSKNVIDENYQLA 610
Query: 549 STQQKKNKDIPEGGSKGVILLNPGLTSSKDTFNAFSQYVDSVIDILIKDPLKENYVDLYG 608
STQQ+KNKDIPEGGSKGVILLNPGL TF AFSQYVD++IDILI DPLKENYV+L
Sbjct: 611 STQQRKNKDIPEGGSKGVILLNPGLVEHDQTFVAFSQYVDAMIDILINDPLKENYVNLLP 670
Query: 609 KEEILFFGPDEGTAGFTNWATLHAKRRGCPWWKSFLTGKTSSLGGIPHDEYGMTSLSVRS 668
KEEILFFGPDEGTAGF +WAT HA+ R CPWWKSFLTGK+ SLGGIPHDEYGMTSL VR+
Sbjct: 671 KEEILFFGPDEGTAGFVDWATNHARVRNCPWWKSFLTGKSPSLGGIPHDEYGMTSLGVRA 730
Query: 669 YVNALADHAGFQNEKTYKFQTGGPDGDLGSNEILLSTENEAYVGLVDGSGVLCDPEGLDK 728
YVN + + N YKFQTGGPDGDLGSNEILLS+ NE Y+ ++DGSGVLCDP+GLDK
Sbjct: 731 YVNKIYETLNLTNSTVYKFQTGGPDGDLGSNEILLSSPNECYLAILDGSGVLCDPKGLDK 790
Query: 729 HELINLAKQRVMVSSYDKSKLSKKGFFVSVDDIDVMLPNGVLVANGTTFRNTFHYEVFKF 788
EL LA +R M+S +D SKLS GFFVSVD +D+MLPNG +VANGTTFRNTFH ++FKF
Sbjct: 791 DELCRLAHERKMISDFDTSKLSNNGFFVSVDAMDIMLPNGTIVANGTTFRNTFHTQIFKF 850
Query: 789 VNHIDLFVPCGGRPNSIDLNNLHYFINKENNKSKIPYIVEGANLFISQPAKLELERHGAI 848
V+H+D+FVPCGGRPNSI LNNLHYF++++ K KIPYIVEGANLFI+QPAK LE HG I
Sbjct: 851 VDHVDIFVPCGGRPNSITLNNLHYFVDEKTGKCKIPYIVEGANLFITQPAKNALEEHGCI 910
Query: 849 LIKDASANKGGVTSSSMEVLAALSLNDEDFVGKFVSTDGKTNTELYKSYVKQIQEKIQFN 908
L KDASANKGGVTSSSMEVLA+L+LND DFV KF+ + LYKSYV ++Q +IQ N
Sbjct: 911 LFKDASANKGGVTSSSMEVLASLALNDNDFVHKFIGDVSGERSALYKSYVVEVQSRIQKN 970
Query: 909 ARQEFGQLWKLNKATGLPMSHLSNVISETINKLNDDLIESDELWSNDLKLRNYLLLNKII 968
A EFGQLW LN+ G +S +SN +S TINKLNDDL+ S ELW NDLKLRNYLLL+KII
Sbjct: 971 AELEFGQLWNLNQLNGTHISEISNQLSFTINKLNDDLVASQELWLNDLKLRNYLLLDKII 1030
Query: 969 PKLLTDVAGPEQILKQIPDXXXXXXXXXXXXXHFVYEFGTDVNMGKFLEYIGNLKREA 1026
PK+L DVAGP+ +L+ IP+ FVY+ G DVN+GKFLE+IG LKREA
Sbjct: 1031 PKILIDVAGPQSVLENIPESYLKVLLSSYLSSTFVYQNGIDVNIGKFLEFIGGLKREA 1088
>SPCC132.04c [E] KOG2250 Glutamate/leucine/phenylalanine/valine dehydrogenases
Length = 1106
Score = 652 bits (1681), Expect = 0.0
Identities = 393/1033 (38%), Positives = 573/1033 (55%), Gaps = 44/1033 (4%)
Query: 20 YYNFEFPGKSAQKEQVVDLL-DQQGFIPDTLLENEVDWFYEGLGIDDYFFSKEVPETIAN 78
Y F GK QK +V+ L ++ IPD L+E+EVD FY LG+DDY+F E IA+
Sbjct: 93 YRPIVFSGKQDQKSKVIQCLRTERKTIPDDLVEDEVDRFYNKLGLDDYYFQMEPVSIIAD 152
Query: 79 VISTLYASKVESFAQN-HQKLQVEKAIINDSHAMFLDTNHHLDTEID----------SKF 127
I +YA+K+ + A + ++L + N+ A++LD++ E+D +++
Sbjct: 153 HIEIIYAAKIAAHASHAKEELNIHVKNENEDLAIYLDSSPVTQPELDQSSAVEESISTRY 212
Query: 128 LD----SEEHHYRLISFLAENSLKLSFVYQNEYPTGNASSIELTDEQLTSG------DID 177
LD ++ YR+ SF + ++ + + T + + D DI
Sbjct: 213 LDPFKLTDPTAYRVESFTSVTNIDRTSTENSNIYTYFVTKCDFVDNPKKDASPDVPTDIA 272
Query: 178 SISDVTMITMTSQENKKLYGQLIHHVSQREGPVIKTFNSVANANEVRMCIAFKRSSTKGX 237
S+SD T + S ++Y +++ V R GPV++ F+ +E+R+ + ++R S
Sbjct: 273 SVSDKTFLEKASDNTIEMYQDVMNSVLTRFGPVVRLFD-YQGRSEIRLVVGYRRGSIFQY 331
Query: 238 XXXXXXXXXXXXXKHSKLYVDTFANDVSIISIYLDKSQQDHDILKELGLSLLQVEREASL 297
++ YV+ F+N V+IIS + + + Q+ REASL
Sbjct: 332 FPSLSKLFRYYGLHSTRTYVEQFSNGVTIISYNFKPELFKNAAVTSINELFSQITREASL 391
Query: 298 LYAIPQNSFKDLYQSRQFSPQESIYAHIGSIFINHFINRLGSSYDSLIRQLNVKPDDTLL 357
LY +P F+ L+ S + S QE YAH IF H +N+LG Y SL L+ + +
Sbjct: 392 LYCLPSTDFQPLFVSEKLSIQEVTYAHCVRIFCEHVMNKLGPEYSSLSAILD--HSNNIH 449
Query: 358 NELLANLKRKLRTETYTQQMIIHVLHKYKEIVSKLYKSFAQVHYPTKTSATGSGNQKLQM 417
E+L +KR+L T +T+ I + +Y +V L++ F H S K
Sbjct: 450 AEILETIKRRLSTLAFTRTKIHDTIMQYPGLVHTLFEQFYLEHAINHNSTPHLHRAKSAT 509
Query: 418 TLSYQRLS-QLEPFQSEEEFNLYLNKVFPNDSPDLLILRTLNLFNKSILKTNFFITRKVA 476
+L+ + + + P + +L + K N+ D+ ++ FN +LKTNFF T KVA
Sbjct: 510 SLADEASTYSITPMSATALMDL-IQKTCTNEE-DVSVMEMFVKFNTHLLKTNFFQTTKVA 567
Query: 477 ISFKLDPALILPKSEYPETPYGIFFVVGNTFKAFHIRFRDISRGGIRIVVSRSQDVYDKN 536
+SF+ DP+ L ++Y + Y + +GN F+ FH+RFRD++RGGIR++ S + + + N
Sbjct: 568 LSFRFDPSF-LDSTQYKDPLYAMIMSIGNEFRGFHLRFRDVARGGIRLIKSANPEAFGLN 626
Query: 537 SKSVIDENYGLASTQQKKNKDIPEGGSKGVILLNPGLTSSKDTFNAFSQYVDSVIDILIK 596
++ + DENY LA TQ KNKDIPEGG+KGVILL + AF +Y+DS+ID+LI
Sbjct: 627 ARGLFDENYNLAKTQMLKNKDIPEGGAKGVILLGKDCQDKPEL--AFMKYIDSIIDLLIV 684
Query: 597 DPLKENYVDLYGKEEILFFGPDEGTAGFTNWATLHAKRRGCPWWKSFLTGKTSSLGGIPH 656
+ + VD GK EILF GPDE TA NWAT+HA RR PWWKSF TGK ++GGIPH
Sbjct: 685 NK-SQPLVDKLGKPEILFMGPDENTADLVNWATIHAHRRNAPWWKSFFTGKKPTMGGIPH 743
Query: 657 DEYGMTSLSVRSYVNALADHAGFQN-EKTYKFQTGGPDGDLGSNEILLSTENEAYVGLVD 715
D+YGMTSLSVR YV + + K K QTGGPDGDLGSNEI LS NE Y+ ++D
Sbjct: 744 DKYGMTSLSVRCYVEGIYKKLNITDPSKLTKVQTGGPDGDLGSNEIKLS--NEKYIAVID 801
Query: 716 GSGVLCDPEGLDKHELINLAKQRVMVSSYDKSKLSKKGFFVSVDDIDVMLPNGVLVANGT 775
GSGVL DP GLD+ EL+ LA +R + +D KLS +G+ V V D ++ LPNG +V NGT
Sbjct: 802 GSGVLYDPAGLDRTELLRLADERKTIDHFDAGKLSPEGYRVLVKDTNLKLPNGEIVRNGT 861
Query: 776 TFRNTFHYEVFKFVNHIDLFVPCGGRPNSIDLNNLHYFINKENNKSKIPYIVEGANLFIS 835
FRNT H D FVPCGGRPN+I++NN+ I+ ++ + Y+VEGANLFI+
Sbjct: 862 IFRNTAHLRY-----KADTFVPCGGRPNAININNVEQLID-DHGRPAFKYLVEGANLFIT 915
Query: 836 QPAKLELERHGAILIKDASANKGGVTSSSMEVLAALSLNDEDFVGKFVSTDGKTNTELYK 895
Q AK LE+ G I+I+DASANKGGVTSSS+EVLA+LS +D F DGK T Y
Sbjct: 916 QDAKSVLEKAGVIVIRDASANKGGVTSSSLEVLASLSFDDASFKENMCVHDGKVPT-FYA 974
Query: 896 SYVKQIQEKIQFNARQEFGQLWKLNKATGLPMSHLSNVISETINKLNDDLIESDELWSND 955
YV +++ IQ NA EF +WK + +P + LSN +S I KL+ D+ ++LW+ D
Sbjct: 975 DYVNEVKRIIQRNANLEFEAIWKGHSENKIPYTSLSNHLSTEIVKLDHDIYNYEKLWA-D 1033
Query: 956 LKLRNYLLLNKIIPKLLTDVAGPEQILKQIPDXXXXXXXXXXXXXHFVYEFGTDVNMGKF 1015
+ RN +L IPK L G E++L++IP+ FVY+ + F
Sbjct: 1034 VGFRN-AVLRASIPKTLQAKIGLEKMLERIPESYLRAIFSTYLASRFVYQHVVSSDPFAF 1092
Query: 1016 LEYIGNLKREASD 1028
+YI + D
Sbjct: 1093 FDYISTEMKMLKD 1105
>At3g03910 [E] KOG2250 Glutamate/leucine/phenylalanine/valine dehydrogenases
Length = 411
Score = 53.9 bits (128), Expect = 1e-06
Identities = 102/432 (23%), Positives = 164/432 (37%), Gaps = 96/432 (22%)
Query: 472 TRKVAISFKLDPALILPKSEY------PETPYGIFFVVGNTFKAFHIRFRDISRGGIRIV 525
+R + + KL+ +L++P E P+ + VG F+ H R +GGIR
Sbjct: 15 SRLLGLDSKLEKSLLIPFREIKVECTIPKDDGTLASFVG--FRVQHDNARGPMKGGIRY- 71
Query: 526 VSRSQDVYDKNSKSVIDENYGLASTQQKKNK--DIPEGGSKGVILLNPG---LTSSKDTF 580
+ + DE LA K IP GG+KG I +P L+ +
Sbjct: 72 ----------HPEVEPDEVNALAQLMTWKTAVAKIPYGGAKGGIGCDPSELSLSELERLT 121
Query: 581 NAFSQYVDSVIDILIKDPLKENYVDLYGKEEILFFGPDEGTAGFTNWATLHAKRRGCPWW 640
F+Q + +I I P PD GT T L +
Sbjct: 122 RVFTQKIHDLIGIHTDVP-----------------APDMGTGPQTMAWILDEYSKFHGHS 164
Query: 641 KSFLTGKTSSLGGIPHDEYGMTSLSVRSYVNA----LADHAGFQNEKTYKFQTGGPDGDL 696
+ +TGK LGG G + + R + A L +H + + + Q G G
Sbjct: 165 PAVVTGKPIDLGG----SLGRDAATGRGVLFATEALLNEHGKTISGQRFAIQGFGNVGSW 220
Query: 697 GSNEILLSTENEAYVGLVDGSGVLCDPEGLDKHELINLAKQRVMVSSYDKSKLSKKGFFV 756
+ L+S + V + D +G + + G+D L+ A++ + +D
Sbjct: 221 AAK--LISDKGGKIVAVSDVTGAIKNNNGIDILSLLEHAEENRGIKGFD----------- 267
Query: 757 SVDDIDVMLPNGVLVANGTTFRNTFHYEVFKFVNHIDLFVPCGGRPNSIDLNNLHYFINK 816
D ID P+ +LV + D+ VP L IN+
Sbjct: 268 GADSID---PDSILVED------------------CDILVPAA----------LGGVINR 296
Query: 817 EN-NKSKIPYIVEGANLFISQPAKLELERHGAILIKDASANKGGVTSSSMEVLAALS--L 873
EN N+ K +I+EGAN A L++ G +++ D AN GGVT S E + + +
Sbjct: 297 ENANEIKAKFIIEGANHPTDPEADEILKKKGVMILPDIYANSGGVTVSYFEWVQNIQGFM 356
Query: 874 NDEDFVGKFVST 885
DE+ V + + T
Sbjct: 357 WDEEKVNRELKT 368
>At5g18170 [E] KOG2250 Glutamate/leucine/phenylalanine/valine dehydrogenases
Length = 411
Score = 53.1 bits (126), Expect = 2e-06
Identities = 99/407 (24%), Positives = 149/407 (36%), Gaps = 88/407 (21%)
Query: 473 RKVAISFKLDPALILPKSEY------PETPYGIFFVVGNTFKAFHIRFRDISRGGIRIVV 526
R + + KL+ +L++P E P+ + VG F+ H R +GGIR
Sbjct: 16 RLLGLDSKLEKSLLIPFREIKVECTIPKDDGTLASFVG--FRVQHDNARGPMKGGIRY-- 71
Query: 527 SRSQDVYDKNSKSVIDENYGLASTQQKKNK--DIPEGGSKGVILLNPGLTSSKDT---FN 581
+ + DE LA K IP GG+KG I +P S +
Sbjct: 72 ---------HPEVDPDEVNALAQLMTWKTAVAKIPYGGAKGGIGCDPSKLSISELERLTR 122
Query: 582 AFSQYVDSVIDILIKDPLKENYVDLYGKEEILFFGPDEGTAGFTNWATLHAKRRGCPWWK 641
F+Q + +I I P PD GT T L + +
Sbjct: 123 VFTQKIHDLIGIHTDVP-----------------APDMGTGPQTMAWILDEYSKFHGYSP 165
Query: 642 SFLTGKTSSLGGIPHDEYGMTSLSVRSYVNALA-DHAGFQNEKTYKFQTGGPDGDLGSNE 700
+ +TGK LGG + T V AL +H + + + Q G G +
Sbjct: 166 AVVTGKPIDLGGSLGRD-AATGRGVMFGTEALLNEHGKTISGQRFVIQGFGNVGSWAAK- 223
Query: 701 ILLSTENEAYVGLVDGSGVLCDPEGLDKHELINLAKQRVMVSSYDKSKLSKKGFFVSVDD 760
L+S + V + D +G + + +G+D L+ K+ V +D D
Sbjct: 224 -LISEKGGKIVAVSDITGAIKNKDGIDIPALLKHTKEHRGVKGFD-----------GADP 271
Query: 761 IDVMLPNGVLVANGTTFRNTFHYEVFKFVNHIDLFVPCGGRPNSIDLNNLHYFINKEN-N 819
ID PN +LV + D+ VP L IN+EN N
Sbjct: 272 ID---PNSILVED------------------CDILVPAA----------LGGVINRENAN 300
Query: 820 KSKIPYIVEGANLFISQPAKLELERHGAILIKDASANKGGVTSSSME 866
+ K +I+E AN A L + G +++ D AN GGVT S E
Sbjct: 301 EIKAKFIIEAANHPTDPDADEILSKKGVVILPDIYANSGGVTVSYFE 347
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,476,306
Number of Sequences: 60738
Number of extensions: 2687474
Number of successful extensions: 6939
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 6915
Number of HSP's gapped (non-prelim): 5
length of query: 1032
length of database: 30,389,216
effective HSP length: 117
effective length of query: 915
effective length of database: 23,282,870
effective search space: 21303826050
effective search space used: 21303826050
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)