ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2137 good U KOG3225 Intracellular trafficking, secretion, and vesicular transport Mitochondrial import inner membrane translocase, subunit TIM22
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2137 728219 728806 196
(196 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL217c [U] KOG3225 Mitochondrial import inner membrane transloc... 301 5e-82
SPBC25H2.04c [U] KOG3225 Mitochondrial import inner membrane tra... 139 3e-33
Hs18588220 [U] KOG3225 Mitochondrial import inner membrane trans... 113 1e-25
Hs7019553 [U] KOG3225 Mitochondrial import inner membrane transl... 102 3e-22
CE25820 [U] KOG3225 Mitochondrial import inner membrane transloc... 102 4e-22
At3g10110 [U] KOG3225 Mitochondrial import inner membrane transl... 75 7e-14
At4g26670 [U] KOG1652 Mitochondrial import inner membrane transl... 58 9e-09
At1g18320 [U] KOG3225 Mitochondrial import inner membrane transl... 57 2e-08
At5g55510 [U] KOG1652 Mitochondrial import inner membrane transl... 57 2e-08
>YDL217c [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 207
Score = 301 bits (770), Expect = 5e-82
Identities = 141/207 (68%), Positives = 174/207 (83%), Gaps = 11/207 (5%)
Query: 1 MVYRGFGLDQVSPPENKPFDQLSPEEQGEKGAQMMVEFMTSCPGKAAISGVTGFALGGVF 60
MVY GFGL+Q+SP + KP+++L+PEEQGE+GA+M++ FMTSCPGK+ +SGVTGFALGGV
Sbjct: 1 MVYTGFGLEQISPAQKKPYNELTPEEQGERGAEMIMNFMTSCPGKSVVSGVTGFALGGVL 60
Query: 61 GLFMASMAYDTPLHTPAPTNAP-----------GLPNKVKELADLPLKQQIKIQFSDMGK 109
GLFMASMAYDTPLHTP P N G+ V++++DLP +QQ+K+QF+DMGK
Sbjct: 61 GLFMASMAYDTPLHTPTPANTAATATAGNIGVGGISRTVQQISDLPFRQQMKLQFTDMGK 120
Query: 110 RSYSSAKNFGYIGMIYSGVECVVESLRAKNDIYNGVAAGCLTGGGLAYKSGPQAALVGCA 169
+SYSSAKNFGYIGMIY+GVECV+ESLRAKNDIYNGV AG TG GLAYK+GPQAAL+G A
Sbjct: 121 KSYSSAKNFGYIGMIYAGVECVIESLRAKNDIYNGVTAGFFTGAGLAYKAGPQAALMGGA 180
Query: 170 GFAAFSTAIDLYMRNENKKPPKDDFDE 196
GFAAFS AIDLYM++E+ +PP++DF E
Sbjct: 181 GFAAFSAAIDLYMKSEDGRPPQNDFKE 207
>SPBC25H2.04c [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 175
Score = 139 bits (349), Expect = 3e-33
Identities = 72/165 (43%), Positives = 105/165 (63%), Gaps = 19/165 (11%)
Query: 22 LSPEEQGEKGAQMMVEFMT----SCPGKAAISGVTGFALGGVFGLFMASMAYDTPLHTPA 77
L+PEE+ ++ +M SC K++++GV GF LGG+FG+F++S+
Sbjct: 22 LTPEEKAALQQAKIIGYMNRISESCVFKSSMAGVLGFGLGGIFGMFISSL---------- 71
Query: 78 PTNAPGLPNKVKELADLPLKQQIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRA 137
L + ++ + P ++QI+IQ DMG RS+S+AKNFG +G+IYSG EC +E+ RA
Sbjct: 72 -----DLQHIDPKIYEKPFREQIRIQARDMGSRSFSTAKNFGLLGLIYSGSECCIEAFRA 126
Query: 138 KNDIYNGVAAGCLTGGGLAYKSGPQAALVGCAGFAAFSTAIDLYM 182
K DIYN +AAG TGG LA +SGP+A ++G AGF FS I+ YM
Sbjct: 127 KTDIYNAIAAGVFTGGALAVRSGPKAIVLGGAGFGLFSYGIEKYM 171
>Hs18588220 [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 194
Score = 113 bits (283), Expect = 1e-25
Identities = 62/161 (38%), Positives = 89/161 (54%), Gaps = 18/161 (11%)
Query: 23 SPEEQGEKGAQMMVEFMTSCPGKAAISGVTGFALGGVFGLFMASMAYDTPLHTPAPTNAP 82
SP + E+ +M+ + M SC KAA++ V GF LGG FG+F A + + P P
Sbjct: 52 SPAKSEEQ--KMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTP 109
Query: 83 GLPNKVKELADLPLKQQIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRAKNDIY 142
+K DMG+R S AKNF +G ++S EC++ES R +D
Sbjct: 110 TAKEVLK----------------DMGQRGMSYAKNFAIVGAMFSCTECLIESYRGTSDWK 153
Query: 143 NGVAAGCLTGGGLAYKSGPQAALVGCAGFAAFSTAIDLYMR 183
N V +GC+TGG + +++G +A +GC GFAAFS AID Y+R
Sbjct: 154 NSVISGCITGGAIGFRAGLKAGAIGCGGFAAFSAAIDYYLR 194
>Hs7019553 [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 194
Score = 102 bits (254), Expect = 3e-22
Identities = 59/161 (36%), Positives = 86/161 (52%), Gaps = 18/161 (11%)
Query: 23 SPEEQGEKGAQMMVEFMTSCPGKAAISGVTGFALGGVFGLFMASMAYDTPLHTPAPTNAP 82
SP + E+ +M+ + M SC KAA++ V GF LGG FG+F A + + P P
Sbjct: 52 SPAKSEEQ--KMIEKAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTP 109
Query: 83 GLPNKVKELADLPLKQQIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRAKNDIY 142
+KE G+R S AKNF +G ++S C++ES R +D+
Sbjct: 110 TAKEVLKE----------------HGQRGMSYAKNFAIVGAMFSCTWCLIESYRGTSDLK 153
Query: 143 NGVAAGCLTGGGLAYKSGPQAALVGCAGFAAFSTAIDLYMR 183
N V +GC+TGG +++G +A + C GFAAFS AID Y+R
Sbjct: 154 NSVISGCITGGAYWFRAGLKAGALLCGGFAAFSAAIDYYLR 194
>CE25820 [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 213
Score = 102 bits (253), Expect = 4e-22
Identities = 64/145 (44%), Positives = 82/145 (56%), Gaps = 16/145 (11%)
Query: 39 MTSCPGKAAISGVTGFALGGVFGLFMASMAYDTPLHTPAPTNAPGLPNKVKELADLPLKQ 98
M +C KA+ISGV G +G FGLF AS+ P + G P K L LKQ
Sbjct: 84 MENCTVKASISGVLGVGVGFAFGLFTASV-------DPQLSMVGGDPTK-----QLTLKQ 131
Query: 99 QIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRAKNDIYNGVAAGCLTGGGLAYK 158
K +M R S KNFG IG+++SG EC +E++RAK+D NG +G + GG L +
Sbjct: 132 TWK----EMSSRMKSYGKNFGSIGLMFSGTECALETIRAKSDWRNGTYSGGIVGGLLGLR 187
Query: 159 SGPQAALVGCAGFAAFSTAIDLYMR 183
+G A+ G AGFA FST ID YMR
Sbjct: 188 AGIMPAVWGAAGFAVFSTIIDHYMR 212
>At3g10110 [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 230
Score = 74.7 bits (182), Expect = 7e-14
Identities = 41/123 (33%), Positives = 63/123 (50%), Gaps = 18/123 (14%)
Query: 37 EFMTSCPGKAAISGVTGFALGGVFGLFMASMAYDTPLHTPAPTNAPGLPNKVKELADLPL 96
E +C +A SGV G LG + GLF+ ++ D P+ T
Sbjct: 42 EVWNNCAVRAVTSGVMGGGLGLMMGLFLGAL--DNPITHDTMT----------------A 83
Query: 97 KQQIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRAKNDIYNGVAAGCLTGGGLA 156
+QQ MG+RS++S K F +G+++S EC+VE RAK+D N AGC+TGG ++
Sbjct: 84 RQQFVFTAKQMGQRSWNSCKTFAVMGLVFSAAECIVEKARAKHDTVNTAIAGCVTGGSMS 143
Query: 157 YKS 159
++
Sbjct: 144 ARA 146
>At4g26670 [U] KOG1652 Mitochondrial import inner membrane translocase subunit
TIM17
Length = 208
Score = 57.8 bits (138), Expect = 9e-09
Identities = 31/83 (37%), Positives = 46/83 (55%), Gaps = 4/83 (4%)
Query: 97 KQQIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRAKNDIYNGVAAGCLTGGGLA 156
K+ K F+D G+ SAK F + ++S V C+++ +R K+D N AGC TG L+
Sbjct: 72 KKGFKGSFADAGQ----SAKTFAVLSGVHSLVVCLLKQIRGKDDAINVGVAGCCTGLALS 127
Query: 157 YKSGPQAALVGCAGFAAFSTAID 179
+ PQA L C F AFS ++
Sbjct: 128 FPGAPQALLQSCLTFGAFSFILE 150
>At1g18320 [U] KOG3225 Mitochondrial import inner membrane translocase subunit
TIM22
Length = 156
Score = 57.0 bits (136), Expect = 2e-08
Identities = 24/46 (52%), Positives = 33/46 (71%)
Query: 136 RAKNDIYNGVAAGCLTGGGLAYKSGPQAALVGCAGFAAFSTAIDLY 181
RAK+D N AGC+TGG ++ + GP+AA +GCAGFA FS I+ +
Sbjct: 106 RAKHDTVNTAIAGCVTGGSMSARGGPKAACIGCAGFAIFSVLIEKF 151
>At5g55510 [U] KOG1652 Mitochondrial import inner membrane translocase subunit
TIM17
Length = 214
Score = 56.6 bits (135), Expect = 2e-08
Identities = 31/83 (37%), Positives = 45/83 (53%), Gaps = 4/83 (4%)
Query: 97 KQQIKIQFSDMGKRSYSSAKNFGYIGMIYSGVECVVESLRAKNDIYNGVAAGCLTGGGLA 156
K+ K F D G+ SAK F + ++S V C+++ +R K+D N AGC TG L+
Sbjct: 74 KKGFKGSFVDAGQ----SAKTFAVLSGVHSLVVCLLKQIRGKDDAINVGVAGCCTGLALS 129
Query: 157 YKSGPQAALVGCAGFAAFSTAID 179
+ PQA L C F AFS ++
Sbjct: 130 FPGAPQAMLQSCLTFGAFSFILE 152
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,661,874
Number of Sequences: 60738
Number of extensions: 516446
Number of successful extensions: 1404
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1389
Number of HSP's gapped (non-prelim): 11
length of query: 196
length of database: 30,389,216
effective HSP length: 101
effective length of query: 95
effective length of database: 24,254,678
effective search space: 2304194410
effective search space used: 2304194410
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)