ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV2167   suspect: LH   M     KOG1268  Cell wall/membrane/envelope biogenesis                         Glucosamine 6-phosphate synthetases, contain amidotransferase and phosphosugar isomerase domains

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV2167 738165 737074 -364 
         (364 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YNL191w [M] KOG1268 Glucosamine 6-phosphate synthetases contain ...   516   e-146

>YNL191w [M] KOG1268 Glucosamine 6-phosphate synthetases contain
           amidotransferase and phosphosugar isomerase domains
          Length = 357

 Score =  516 bits (1328), Expect = e-146
 Identities = 259/362 (71%), Positives = 285/362 (78%), Gaps = 18/362 (4%)

Query: 1   MCRFLIYKGKEPIKLSHLLTRPEHSIIKQSFDSRLRIDRTRPINGDGFGVAYYSEDPEL- 59
           MCRFLI+KGK+PI+LSHLLTRP HSII QSFDSRLR+DR RP+NGDGFGVAYY  D EL 
Sbjct: 1   MCRFLIFKGKQPIRLSHLLTRPAHSIINQSFDSRLRLDRRRPMNGDGFGVAYYPLDTELS 60

Query: 60  -DGPCLFKAITPAWNNQNLETLAEKTKSRLVLAHVRASTYGTLSETNCHPFTYHNLTFMH 118
            DGPCLFKAITPAWNNQNL TLAEKTKS LV AHVRASTYG LSETNCHPFTYH+L FMH
Sbjct: 61  EDGPCLFKAITPAWNNQNLSTLAEKTKSDLVFAHVRASTYGVLSETNCHPFTYHSLCFMH 120

Query: 119 NGGIGNFHRFKRKLLNHIGDKYLLMIQGSTDSECAFALLLDTLERMGYDPASEQQRVXXX 178
           NGGI NF   KRKLLNHI D+YL  IQGSTDSECAFAL LDTL+++GYDP  +       
Sbjct: 121 NGGISNFKGIKRKLLNHIKDEYLNFIQGSTDSECAFALFLDTLDKLGYDPKKQD------ 174

Query: 179 XXXXXXXXXXXXXNHVIIREALLKTIDLLKQWTQEAADSSDTFEPSLLNFAVTDGDCVVI 238
                         +V +R+A+L+TID ++ WT+EA       EPSLLNFAVTDG  VV+
Sbjct: 175 ----------GDFGNVALRKAMLRTIDYIRDWTKEANKDEAHVEPSLLNFAVTDGSTVVV 224

Query: 239 SRYITSSTDEAPSLHFSCGSSFVESGPGEYRMERLDRNQDVIMVASEPLTFERGDWTAVP 298
           SRYITS TDEA SLHFSCGSSFVE+ PGEYR+ERLDRNQDVIMVASEPLTFERGDWTAVP
Sbjct: 225 SRYITSKTDEAASLHFSCGSSFVETSPGEYRVERLDRNQDVIMVASEPLTFERGDWTAVP 284

Query: 299 TNSILTIRNQTVLLHPVIDEYYQADPLYARSSTLAESKGLMGXXXXXXXXXXXXXXLERD 358
           TNSILTI+ QT+LLHP+IDEYYQ DPLY RSSTLAESKGLMG              LER+
Sbjct: 285 TNSILTIKKQTILLHPIIDEYYQEDPLYLRSSTLAESKGLMGSIPLAKAVEKNVPPLERE 344

Query: 359 RR 360
            R
Sbjct: 345 GR 346


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.320    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,906,049
Number of Sequences: 60738
Number of extensions: 793656
Number of successful extensions: 1542
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1539
Number of HSP's gapped (non-prelim): 1
length of query: 364
length of database: 30,389,216
effective HSP length: 108
effective length of query: 256
effective length of database: 23,829,512
effective search space: 6100355072
effective search space used: 6100355072
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)