ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV2187   suspect: LH   S     KOG4562  Function unknown                                               Uncharacterized conserved protein (tumor-rejection antigen MAGE in humans)

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV2187 742932 742012 -307 
         (307 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR288w [S] KOG4562 Uncharacterized conserved protein (tumor-rej...   134   1e-31

>YDR288w [S] KOG4562 Uncharacterized conserved protein (tumor-rejection
           antigen MAGE in humans)
          Length = 303

 Score =  134 bits (338), Expect = 1e-31
 Identities = 85/285 (29%), Positives = 153/285 (52%), Gaps = 11/285 (3%)

Query: 19  VGNLMVRFVLAQCESQTGNTVITRSKMLQKLKEIIDEEKVKSIPFSTVYGYVDDKLNEIF 78
           V   MVR++L++ ESQ  N++ITR+K+   + E   EE +    FS ++  ++  L  ++
Sbjct: 27  VARKMVRYILSRGESQ--NSIITRNKLQSVIHEAAREENIAKPSFSKMFMDINAILYNVY 84

Query: 79  GYHLFGVLAKKTDKRSFSQTQDEGTPDEANNSV-EDAKARGNHFILVRSGMEAIPQRYHK 137
           G+ L G+ +K             G+    N S+ E    R   FIL+ +   +  + +  
Sbjct: 85  GFELQGLPSKNN-----MNAGGNGSNSNTNKSMPEPLGHRAQKFILLNNVPHS--KNFDD 137

Query: 138 FILDHASAIYRKTVHQVTYIGKEYSASSNPTLDNEIGNNTELALKGIIAITICIVIFSKN 197
           F +  ++  Y + +    YIG + ++ ++ TL++++  + +L  KG++++ +CIV FSKN
Sbjct: 138 FKILQSAHTYEELIVTGEYIGDDIASGTSNTLESKLSTDRDLVYKGVLSVILCIVFFSKN 197

Query: 198 NILETELSDQHGKFGISTEGHDIPIIDMNHKDLLNLLVRNNYLTRTVEKAADLGQELAIY 257
           NIL  EL      FGI ++G  I I+++  +DL+  L +  Y+ R  EK +D   E+  Y
Sbjct: 198 NILHQELIKFLETFGIPSDGSKIAILNITIEDLIKSLEKREYIVRLEEK-SDTDGEVISY 256

Query: 258 SIGKRTLVEFPKESLTKMCQEMLDLDDSQLELIENTVNVLAGDAY 302
            IG+RT  E   ESL K+ QE++ L+  Q + + + +    GD+Y
Sbjct: 257 RIGRRTQAELGLESLEKLVQEIMGLEKEQTKSLHDDIIKSIGDSY 301


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.134    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,874,138
Number of Sequences: 60738
Number of extensions: 741612
Number of successful extensions: 2122
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2119
Number of HSP's gapped (non-prelim): 1
length of query: 307
length of database: 30,389,216
effective HSP length: 106
effective length of query: 201
effective length of database: 23,950,988
effective search space: 4814148588
effective search space used: 4814148588
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)