ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2199 good I KOG1459 Lipid transport and metabolism Squalene synthetase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2199 745975 747297 441
(441 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHR190w [I] KOG1459 Squalene synthetase 570 e-162
SPBC646.05c [I] KOG1459 Squalene synthetase 324 2e-88
Hs4758350 [I] KOG1459 Squalene synthetase 293 3e-79
At4g34640 [I] KOG1459 Squalene synthetase 256 5e-68
At4g34650 [I] KOG1459 Squalene synthetase 234 1e-61
>YHR190w [I] KOG1459 Squalene synthetase
Length = 444
Score = 570 bits (1470), Expect = e-162
Identities = 281/400 (70%), Positives = 326/400 (81%), Gaps = 1/400 (0%)
Query: 39 LTRCHELLKLTSRSFAAVIMELHPELRNAVMLFYLVLRALDTVEDDMTIDPKIKIPVLRE 98
L C ELL LTSRSFAAVI ELHPELRN V LFYL+LRALDT+EDDM+I+ +KI +LR
Sbjct: 39 LLHCFELLNLTSRSFAAVIRELHPELRNCVTLFYLILRALDTIEDDMSIEHDLKIDLLRH 98
Query: 99 FDSKLDLPSWYFDGNGPNEKDRAVLVDFPCILNELHKLKPEYQRVIKEITGKMGNGMADY 158
F KL L W FDGN P+ KDRAVL DF IL E HKLKPEYQ VIKEIT KMGNGMADY
Sbjct: 99 FHEKLLLTKWSFDGNAPDVKDRAVLTDFESILIEFHKLKPEYQEVIKEITEKMGNGMADY 158
Query: 159 ILDENFNLNGLQTVADYDLYCHYVAGLVGDGLTQMLVLAGFGSNDLYDENVHLYEDMGLF 218
ILDEN+NLNGLQTV DYD+YCHYVAGLVGDGLT+++V+A F + LY N LYE MGLF
Sbjct: 159 ILDENYNLNGLQTVHDYDVYCHYVAGLVGDGLTRLIVIAKFANESLYS-NEQLYESMGLF 217
Query: 219 LQKTNIIRDYAEDLADGRSFWPKEIWSLYAEKLSDFAKPENTQQGLYCINHLVLNAMTHI 278
LQKTNIIRDY EDL DGRSFWPKEIWS YA +L DF KPEN Q GL CINHLVLNA++H+
Sbjct: 218 LQKTNIIRDYNEDLVDGRSFWPKEIWSQYAPQLKDFMKPENEQLGLDCINHLVLNALSHV 277
Query: 279 ESVLHYLASIHEQSSFQFCAIPQVMAIATLSLVFANPKVMHENVKIRKGTTCYLILKSRT 338
VL YLA IHEQS+FQFCAIPQVMAIATL+LVF N +V+H NVKIRKGTTCYLILKSRT
Sbjct: 278 IDVLTYLAGIHEQSTFQFCAIPQVMAIATLALVFNNREVLHGNVKIRKGTTCYLILKSRT 337
Query: 339 LRGCVEIFQYYLRDIKKRLTVEDPSYLKLNIQISKIEQYIESMYQEQLPLGVKPQETLIY 398
LRGCVEIF YYLRDIK +L V+DP++LKLNIQISKIEQ++E MYQ++LP VKP ET I+
Sbjct: 338 LRGCVEIFDYYLRDIKSKLAVQDPNFLKLNIQISKIEQFMEEMYQDKLPPNVKPNETPIF 397
Query: 399 KKVLDRTEYDSKVAVIEEDEEFKFNLGFTMFLTFIAAVYF 438
KV +R+ YD ++ +++EE+KFN+ ++ L+ + Y+
Sbjct: 398 LKVKERSRYDDELVPTQQEEEYKFNMVLSIILSVLLGFYY 437
>SPBC646.05c [I] KOG1459 Squalene synthetase
Length = 460
Score = 324 bits (830), Expect = 2e-88
Identities = 165/346 (47%), Positives = 225/346 (64%), Gaps = 2/346 (0%)
Query: 38 DLTRCHELLKLTSRSFAAVIMELHPELRNAVMLFYLVLRALDTVEDDMTIDPKIKIPVLR 97
++ RC++LL +TSRSFA VI EL +R AVM+FYLVLR LDTVEDDMT+ K+P+LR
Sbjct: 34 NIRRCYQLLDMTSRSFAVVIKELPNGIREAVMIFYLVLRGLDTVEDDMTLPLDKKLPILR 93
Query: 98 EFDSKLDLPSWYFDGNGPNEKDRAVLVDFPCILNELHKLKPEYQRVIKEITGKMGNGMAD 157
+F +++ W F+ +GPNEKDR +LV+F ++ E L Y+ VI IT +MG+GMA
Sbjct: 94 DFYKTIEVEGWTFNESGPNEKDRQLLVEFDVVIKEYLNLSEGYRNVISNITKEMGDGMAY 153
Query: 158 YI-LDENFNLNGLQTVADYDLYCHYVAGLVGDGLTQMLVLAGFGSNDLYDENVHLYEDMG 216
Y L E + ++T+ D++ YCHYVAGLVG GL+++ + DL + +G
Sbjct: 154 YASLAEKNDGFSVETIEDFNKYCHYVAGLVGIGLSRLFAQSKLEDPDLAHSQA-ISNSLG 212
Query: 217 LFLQKTNIIRDYAEDLADGRSFWPKEIWSLYAEKLSDFAKPENTQQGLYCINHLVLNAMT 276
LFLQK NIIRDY ED D R FWP+EIWS Y D P+N+++ L C++ + NA+T
Sbjct: 213 LFLQKVNIIRDYREDFDDNRHFWPREIWSKYTSSFGDLCLPDNSEKALECLSDMTANALT 272
Query: 277 HIESVLHYLASIHEQSSFQFCAIPQVMAIATLSLVFANPKVMHENVKIRKGTTCYLILKS 336
H L YL+ + Q F FCAIPQVMAIATL+ VF NP V NVKIRKG +IL S
Sbjct: 273 HATDALVYLSQLKTQEIFNFCAIPQVMAIATLAAVFRNPDVFQTNVKIRKGQAVQIILHS 332
Query: 337 RTLRGCVEIFQYYLRDIKKRLTVEDPSYLKLNIQISKIEQYIESMY 382
L+ ++F Y RDI + T +DP++LK++I+ KIEQ ES++
Sbjct: 333 VNLKNVCDLFLRYTRDIHYKNTPKDPNFLKISIECGKIEQVSESLF 378
>Hs4758350 [I] KOG1459 Squalene synthetase
Length = 417
Score = 293 bits (751), Expect = 3e-79
Identities = 166/352 (47%), Positives = 216/352 (61%), Gaps = 16/352 (4%)
Query: 36 SADLTRCHELLKLTSRSFAAVIMELHPELRNAVMLFYLVLRALDTVEDDMTIDPKIKIPV 95
S+ L C++ L TSRSFAAVI L E+RNAV +FYLVLRALDT+EDDMTI + K+P+
Sbjct: 37 SSSLKTCYKYLNQTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPL 96
Query: 96 LREFDSKLDLPSWYFDGNGPNEKDRAVLVDFPCILNELHKLKPEYQRVIKEITGKMGNGM 155
L F S L P W F + EKDR VL DFP I E L +YQ VI +I +MG GM
Sbjct: 97 LHNFHSFLYQPDWRFMES--KEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMGIGM 154
Query: 156 ADYILDENFNLNGLQTVADYDLYCHYVAGLVGDGLTQMLVLAGFGSNDLYDENVHLYEDM 215
A++ LD++ + + ++D YCHYVAGLVG GL+++ + F + L E+ M
Sbjct: 155 AEF-LDKH-----VTSEQEWDKYCHYVAGLVGIGLSRLFSASEF-EDPLVGEDTERANSM 207
Query: 216 GLFLQKTNIIRDYAEDLADGRSFWPKEIWSLYAEKLSDFAKPENTQQGLYCINHLVLNAM 275
GLFLQKTNIIRDY ED GR FWP+E+WS Y +KL DFAKPEN + C+N L+ NA+
Sbjct: 208 GLFLQKTNIIRDYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLAVQCLNELITNAL 267
Query: 276 THIESVLHYLASIHEQSSFQFCAIPQVMAIATLSLVFANPKVMHENVKIRKGTTCYLILK 335
HI V+ YL+ + QS F FCAIPQVMAIATL+ + N +V VKIRKG L++
Sbjct: 268 HHIPDVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGAVKIRKGQAVTLMMD 327
Query: 336 SRTLRGCVEIFQYYLRDIKKRLTVEDPSYLKLNIQISKIEQYIESMYQEQLP 387
+ + I Y+ +I R+ DPS SK Q I ++ + LP
Sbjct: 328 ATNMPAVKAIIYQYMEEIYHRIPDSDPS-------SSKTRQIISTIRTQNLP 372
>At4g34640 [I] KOG1459 Squalene synthetase
Length = 410
Score = 256 bits (654), Expect = 5e-68
Identities = 144/339 (42%), Positives = 207/339 (60%), Gaps = 15/339 (4%)
Query: 42 CHELLKLTSRSFAAVIMELHPELRNAVMLFYLVLRALDTVEDDMTIDPKIKIPVLREFDS 101
C+ +L SRSF+ VI +L+ ELRNAV +FYLVLRALDTVEDD +I K+P+L F
Sbjct: 40 CYSMLHKVSRSFSLVIQQLNTELRNAVCVFYLVLRALDTVEDDTSIPTDEKVPILIAFHR 99
Query: 102 KLDLPSWYFDGNGPNEKDRAVLVD-FPCILNELHKLKPEYQRVIKEITGKMGNGMADYIL 160
+ W++ K+ +L+D F + +L+ YQ I+EIT +MG GMA +I
Sbjct: 100 HIYDTDWHYSCG---TKEYKILMDQFHHVSAAFLELEKGYQEAIEEITRRMGAGMAKFIC 156
Query: 161 DENFNLNGLQTVADYDLYCHYVAGLVGDGLTQMLVLAGFGSNDLYDENVHLYEDMGLFLQ 220
E ++TV DYD YCHYVAGLVG GL+++ + AG S L + + MGLFLQ
Sbjct: 157 QE------VETVDDYDEYCHYVAGLVGLGLSKLFLAAG--SEVLTPDWEAISNSMGLFLQ 208
Query: 221 KTNIIRDYAEDLAD---GRSFWPKEIWSLYAEKLSDFAKPENTQQGLYCINHLVLNAMTH 277
KTNIIRDY ED+ + R FWP+EIW YA+KL D ENT + + C+N +V NA+ H
Sbjct: 209 KTNIIRDYLEDINEIPKSRMFWPREIWGKYADKLEDLKYEENTNKSVQCLNEMVTNALMH 268
Query: 278 IESVLHYLASIHEQSSFQFCAIPQVMAIATLSLVFANPKVMHENVKIRKGTTCYLILKSR 337
IE L Y+ S+ + S F+FCAIPQ+MAI TL+L + N +V VK+R+G T +I +++
Sbjct: 269 IEDCLKYMVSLRDPSIFRFCAIPQIMAIGTLALCYNNEQVFRGVVKLRRGLTAKVIDRTK 328
Query: 338 TLRGCVEIFQYYLRDIKKRLTVEDPSYLKLNIQISKIEQ 376
T+ F + +K ++ DP+ K ++ +++
Sbjct: 329 TMADVYGAFYDFSCMLKTKVDKNDPNASKTLNRLEAVQK 367
>At4g34650 [I] KOG1459 Squalene synthetase
Length = 378
Score = 234 bits (598), Expect = 1e-61
Identities = 131/343 (38%), Positives = 203/343 (58%), Gaps = 15/343 (4%)
Query: 39 LTRCHELLKLTSRSFAAVIMELHPELRNAVMLFYLVLRALDTVEDDMTIDPKIKIPVLRE 98
L C+ +L S+SF+ VI +L ELRNAV +FYL+LRALDTVEDD ++ +IK+P+L
Sbjct: 37 LAFCYSILHKVSKSFSLVIQQLGTELRNAVCVFYLILRALDTVEDDTSVPVEIKVPILIA 96
Query: 99 FDSKLDLPSWYFDGNGPNEKDRAVLVD-FPCILNELHKLKPEYQRVIKEITGKMGNGMAD 157
F + W+F K+ +L+D F + KL+ YQ I++IT +MG GMA
Sbjct: 97 FHRHIYDGDWHFSCG---TKEYKLLMDQFHHVSAAFLKLEKGYQEAIEDITKRMGAGMAK 153
Query: 158 YILDENFNLNGLQTVADYDLYCHYVAGLVGDGLTQMLVLAGFGSNDLYDENVHLYEDMGL 217
+I E ++T+ DYD YCHY AGLVG GL+++ + + L + + GL
Sbjct: 154 FICKE------VETIDDYDEYCHYAAGLVGLGLSKIFIASEL--EILTPDWKQISNSTGL 205
Query: 218 FLQKTNIIRDYAEDLAD---GRSFWPKEIWSLYAEKLSDFAKPENTQQGLYCINHLVLNA 274
FLQKTNII+DY ED+ + R FWP+EIW Y +KL DF E + + C+N +V NA
Sbjct: 206 FLQKTNIIKDYLEDINERPKSRMFWPREIWGKYVDKLEDFKNEEKATKAVQCLNEMVTNA 265
Query: 275 MTHIESVLHYLASIHEQSSFQFCAIPQVMAIATLSLVFANPKVMHENVKIRKGTTCYLIL 334
+ H+E L LAS+ + + FQ CAIPQ++AI TL+L + N +V V++R+G +I
Sbjct: 266 LNHVEDCLKSLASLRDPAIFQSCAIPQIVAIGTLALCYNNVQVFRGVVRLRRGLIAKVID 325
Query: 335 KSRTLRGCVEIFQYYLRDIKKRLTVEDPSYLKLNIQISKIEQY 377
+++T+ F + ++ ++ DP+ +K ++ I+++
Sbjct: 326 RTKTMDDVYGAFYDFSCMLQTKVDNNDPNAMKTLNRLETIKKF 368
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.140 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,739,195
Number of Sequences: 60738
Number of extensions: 1083292
Number of successful extensions: 2621
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2602
Number of HSP's gapped (non-prelim): 5
length of query: 441
length of database: 30,389,216
effective HSP length: 109
effective length of query: 332
effective length of database: 23,768,774
effective search space: 7891232968
effective search space used: 7891232968
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)