ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2344 good DZ KOG3285 Cell cycle control, cell division, chromosome partitioning Spindle assembly checkpoint protein
r_klactIV2344 good DZ KOG3285 Cytoskeleton Spindle assembly checkpoint protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2344 799044 798457 -196
(196 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL030w [DZ] KOG3285 Spindle assembly checkpoint protein 281 5e-76
SPBC20F10.06 [DZ] KOG3285 Spindle assembly checkpoint protein 191 7e-49
At3g25980 [DZ] KOG3285 Spindle assembly checkpoint protein 157 1e-38
Hs4505067 [DZ] KOG3285 Spindle assembly checkpoint protein 156 2e-38
CE26559 [DZ] KOG3285 Spindle assembly checkpoint protein 145 4e-35
7295424 [DZ] KOG3285 Spindle assembly checkpoint protein 134 6e-32
Hs20562718 [DZ] KOG3285 Spindle assembly checkpoint protein 71 8e-13
Hs6006020 [D] KOG3186 Mitotic spindle checkpoint protein 56 3e-08
SPBC12D12.09 [D] KOG3186 Mitotic spindle checkpoint protein 52 7e-07
7296721 [D] KOG3186 Mitotic spindle checkpoint protein 48 7e-06
>YJL030w [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 196
Score = 281 bits (718), Expect = 5e-76
Identities = 138/193 (71%), Positives = 160/193 (82%), Gaps = 1/193 (0%)
Query: 4 ISLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILR 63
ISLKGSTR VTEFFEYSINSILYQR VYPAEDF TVKKYDL+LLKT D ELK YIR+IL
Sbjct: 5 ISLKGSTRTVTEFFEYSINSILYQRGVYPAEDFVTVKKYDLTLLKTHDDELKDYIRKILL 64
Query: 64 QVHRWLLGGKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNIPLEETQKEIRA 123
QVHRWLLGGKC+QLVLCIVDKD+GE +ERW FNV S ++ DD+ + L TQ +IRA
Sbjct: 65 QVHRWLLGGKCNQLVLCIVDKDEGEVVERWSFNVQHISGNSNGQDDV-VDLNTTQSQIRA 123
Query: 124 VLRQITASVTFLPELKNEGNYTFNVLAYTDSNANVPLEWTDSDSKEIAKGESVQFKSFGT 183
++RQIT+SVTFLPEL EG YTF VLAYTD++A VPLEW DS+SKEI GE VQFK+F T
Sbjct: 124 LIRQITSSVTFLPELTKEGGYTFTVLAYTDADAKVPLEWADSNSKEIPDGEVVQFKTFST 183
Query: 184 SAYSVGAEVTYKF 196
+ + VGA+V+YK+
Sbjct: 184 NDHKVGAQVSYKY 196
>SPBC20F10.06 [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 203
Score = 191 bits (484), Expect = 7e-49
Identities = 93/197 (47%), Positives = 135/197 (68%), Gaps = 13/197 (6%)
Query: 5 SLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILRQ 64
SLKGS+++V+EFFEY++NSIL+QR +YPAEDF V+KY L++L + D E+K YIR+I+ Q
Sbjct: 11 SLKGSSKLVSEFFEYAVNSILFQRGIYPAEDFKVVRKYGLNMLVSVDEEVKTYIRKIVSQ 70
Query: 65 VHRWLLGGKCHQLVLCIVDKDDGETIERWKFNV------DSHSNKNDTDDDMNIPLEETQ 118
+H+W+ K +L+L I K GE +ERW+FNV D N + +D++ + Q
Sbjct: 71 LHKWMFAKKIQKLILVITSKCSGEDLERWQFNVEMVDTADQFQNIGNKEDELRV-----Q 125
Query: 119 KEIRAVLRQITASVTFLPELKNEGNYTFNVLAYTDSNANVPLEWTDSDSKEIAKGESVQF 178
KEI+A++RQITA+VTFLP+L E TFNVL Y D ++ VP +W DSD + + E VQ
Sbjct: 126 KEIQALIRQITATVTFLPQL--EEQCTFNVLVYADKDSEVPTDWVDSDPRILRDAEQVQL 183
Query: 179 KSFGTSAYSVGAEVTYK 195
+SF TS + + +V Y+
Sbjct: 184 RSFSTSMHKIDCQVAYR 200
>At3g25980 [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 209
Score = 157 bits (396), Expect = 1e-38
Identities = 81/194 (41%), Positives = 117/194 (59%), Gaps = 2/194 (1%)
Query: 2 DLISLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREI 61
D+I+L GS IV+EFF Y+ NSILY RAVYP E F VKKY L +L +D +K ++ +
Sbjct: 10 DIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDESVKSFMSNL 69
Query: 62 LRQVHRWLLGGKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNIPLEETQKEI 121
Q+ WL GK ++VL I+ K GE +ERW F +++ + D +E +EI
Sbjct: 70 TSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREKSDKEIMREI 129
Query: 122 RAVLRQITASVTFLPELKNEGNYTFNVLAYTDSNANVPLEWTDSDSKEIAKGESVQFKSF 181
+A++RQ+ +SVT+LP L + F+VLAYTD++ VP W +SD K IA + V+ F
Sbjct: 130 QAIMRQVASSVTYLPCL--DETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQMVKLHGF 187
Query: 182 GTSAYSVGAEVTYK 195
T + V V+YK
Sbjct: 188 DTKIHKVDTLVSYK 201
>Hs4505067 [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 205
Score = 156 bits (394), Expect = 2e-38
Identities = 85/196 (43%), Positives = 126/196 (63%), Gaps = 10/196 (5%)
Query: 4 ISLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILR 63
I+L+GS IV EFF + INSILYQR +YP+E F V+KY L+LL T D EL +Y+ ++
Sbjct: 11 ITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVE 70
Query: 64 QVHRWLLGGKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNIPLEETQK---- 119
Q+ WL +LV+ I + + GE +ERW+F+++ + T D + P E++QK
Sbjct: 71 QLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIEC----DKTAKDDSAPREKSQKAIQD 126
Query: 120 EIRAVLRQITASVTFLPELKNEGNYTFNVLAYTDSNANVPLEWTDSDSKEIAKGESVQFK 179
EIR+V+RQITA+VTFLP L E + +F++L YTD + VP +W +S + I E V+ +
Sbjct: 127 EIRSVIRQITATVTFLPLL--EVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLR 184
Query: 180 SFGTSAYSVGAEVTYK 195
SF T+ + V + V YK
Sbjct: 185 SFTTTIHKVNSMVAYK 200
>CE26559 [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 203
Score = 145 bits (365), Expect = 4e-35
Identities = 78/193 (40%), Positives = 121/193 (62%), Gaps = 3/193 (1%)
Query: 4 ISLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILR 63
ISLKGS ++V EFF + +NSILYQRA+YP++ F KKY L+L + +L+ ++ +L+
Sbjct: 10 ISLKGSAQLVKEFFHFGLNSILYQRALYPSDSFKREKKYGLTLWVAHEKKLQAFMDPLLQ 69
Query: 64 QVHRWLLGGKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNIPLE-ETQKEIR 122
QV WL + +LV+ I + E +ERW+F++ + + + ++ + E + ++EI
Sbjct: 70 QVEYWLAKRQLKRLVMVISEVKTKEVVERWQFDIHTENLAEEGENAHRVKEEKKIRQEIS 129
Query: 123 AVLRQITASVTFLPELKNEGNYTFNVLAYTDSNANVPLEWTDSDSKEIAKGESVQFKSFG 182
V+RQITASV+FLP L E +F+VL YT + P +WT+S + I E+VQ +SF
Sbjct: 130 DVIRQITASVSFLPLL--EEPVSFDVLIYTGKDTQAPEDWTESGACLIQNSETVQLRSFS 187
Query: 183 TSAYSVGAEVTYK 195
TS +SV V YK
Sbjct: 188 TSVHSVNTNVQYK 200
>7295424 [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 207
Score = 134 bits (338), Expect = 6e-32
Identities = 68/196 (34%), Positives = 114/196 (57%), Gaps = 6/196 (3%)
Query: 4 ISLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILR 63
I+LKGS +I+ E+ +Y INSIL+QR +YPAEDF ++Y L++L ++D ++K +++ +L
Sbjct: 11 ITLKGSAQIIVEYLKYGINSILFQRGIYPAEDFNNTQQYGLTILMSKDPKIKTFLQNVLS 70
Query: 64 QVHRWLLGGKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNI----PLEETQK 119
Q WL +++ + I + E +E W FN+ + D D L Q
Sbjct: 71 QTEEWLSKNMINKISMVITNAHTKEVLECWDFNMQAELGDGDISDPTKATTTKELSRIQN 130
Query: 120 EIRAVLRQITASVTFLPELKNEGNYTFNVLAYTDSNANVPLEWTDSDSKEIAKGESVQFK 179
EIR V+RQI+A+V++LP L + TF+++ +T N +P +W ++ + I ++VQ +
Sbjct: 131 EIRDVMRQISATVSYLPLL--DCICTFDIMIHTLQNTELPAKWDETGAIVIQNPQAVQLR 188
Query: 180 SFGTSAYSVGAEVTYK 195
SF T + V V YK
Sbjct: 189 SFSTGLHKVDTVVNYK 204
>Hs20562718 [DZ] KOG3285 Spindle assembly checkpoint protein
Length = 130
Score = 71.2 bits (173), Expect = 8e-13
Identities = 34/93 (36%), Positives = 55/93 (58%)
Query: 4 ISLKGSTRIVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILR 63
I+L S +IV +FF + INSIL+Q +YP+ F V KY L+LL T + EL +++ +
Sbjct: 23 ITLCRSAKIVVKFFSFGINSILFQHGIYPSGTFTPVWKYGLTLLVTTNLELMKHLNNTVE 82
Query: 64 QVHRWLLGGKCHQLVLCIVDKDDGETIERWKFN 96
Q+ WL +L+ I + E ++RW+F+
Sbjct: 83 QLKHWLYKRSGQKLIGVIPTTESDEVLQRWQFD 115
>Hs6006020 [D] KOG3186 Mitotic spindle checkpoint protein
Length = 211
Score = 56.2 bits (134), Expect = 3e-08
Identities = 42/150 (28%), Positives = 73/150 (48%), Gaps = 6/150 (4%)
Query: 12 IVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILRQVHRWLLG 71
++ EF E +++ ILY R VYP F KKY++ + + EL QYI++ L V L
Sbjct: 18 VLCEFLEVAVHLILYVREVYPVGIFQKRKKYNVPVQMSCHPELNQYIQDTLHCVKPLLEK 77
Query: 72 GKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNIPLEETQKEIRAVLRQITAS 131
++V+ I+DK+ +E++ F + + + D + L ++ +RA + +I+
Sbjct: 78 NDVEKVVVVILDKEH-RPVEKFVFEITQPPLLSISSDSL---LSHVEQLLRAFILKISVC 133
Query: 132 VTFLPELKNEGNYTFNVLAYTDSNANVPLE 161
L N TF VL +T A +E
Sbjct: 134 DAVLDH--NPPGCTFTVLVHTREAATRNME 161
>SPBC12D12.09 [D] KOG3186 Mitotic spindle checkpoint protein
Length = 207
Score = 51.6 bits (122), Expect = 7e-07
Identities = 28/80 (35%), Positives = 47/80 (58%), Gaps = 1/80 (1%)
Query: 20 SINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILRQVHRWLLGGKCHQLVL 79
SI+ ILY R +YP + F +KY+ + +++ L +YI E+++ L G HQ+ L
Sbjct: 14 SIHCILYARRLYPQDLFIKARKYNTIVWQSRHPILCEYIEEVVQSCIEELQTGSVHQVAL 73
Query: 80 CIVDKDDGETIERWKFNVDS 99
I++K+ E ER+ F+ DS
Sbjct: 74 SIINKEQREE-ERYVFSTDS 92
>7296721 [D] KOG3186 Mitotic spindle checkpoint protein
Length = 198
Score = 48.1 bits (113), Expect = 7e-06
Identities = 35/145 (24%), Positives = 68/145 (46%), Gaps = 6/145 (4%)
Query: 12 IVTEFFEYSINSILYQRAVYPAEDFATVKKYDLSLLKTQDTELKQYIREILRQVHRWLLG 71
I+ E E +N ILY R +YP+ F + Y+ + + L Y+ +L+ L
Sbjct: 9 IIVEAMEVLVNHILYVRGIYPSHIFKMKRMYNSPIYVSIFPPLNNYLAGVLKSAQELLRR 68
Query: 72 GKCHQLVLCIVDKDDGETIERWKFNVDSHSNKNDTDDDMNIPLEETQKEIRAVLRQITAS 131
+ L L + K++ E +E +K +++ + +D L E ++ +R+V+ +I+
Sbjct: 69 RELQCLELIVYQKEN-EKLESYKMQLETQRSGLPAEDH----LMEFEQNMRSVIYKISQR 123
Query: 132 VTFLPELKNEGNYTFNVLAYTDSNA 156
+ P+L G+ F V +T A
Sbjct: 124 LNQAPKLP-AGSCQFKVHLHTTQEA 147
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,911,038
Number of Sequences: 60738
Number of extensions: 520592
Number of successful extensions: 1803
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1786
Number of HSP's gapped (non-prelim): 10
length of query: 196
length of database: 30,389,216
effective HSP length: 101
effective length of query: 95
effective length of database: 24,254,678
effective search space: 2304194410
effective search space used: 2304194410
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)