ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2459 good S KOG2866 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2459 839356 838202 -385
(385 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL105w [S] KOG2866 Uncharacterized conserved protein 323 2e-88
Hs8923009 [S] KOG2866 Uncharacterized conserved protein 74 3e-13
7297703 [S] KOG2866 Uncharacterized conserved protein 61 3e-09
CE18820 [S] KOG2866 Uncharacterized conserved protein 58 3e-08
SPBC20F10.04c [S] KOG2866 Uncharacterized conserved protein 57 5e-08
At1g51130 [S] KOG2866 Uncharacterized conserved protein 54 5e-07
ECU08g1820 [S] KOG2866 Uncharacterized conserved protein 53 7e-07
>YDL105w [S] KOG2866 Uncharacterized conserved protein
Length = 402
Score = 323 bits (829), Expect = 2e-88
Identities = 170/384 (44%), Positives = 245/384 (63%), Gaps = 9/384 (2%)
Query: 5 GNRRSSKRRRTEAFAVKVDKEEERKEFDIIQRYRALEHEVQHDRLQMTRDGDVRVMSKRL 64
G R K+ R++ + + + EF ++Q YR LE E+ R Q+TR GD+ V L
Sbjct: 22 GETRKQKKSRSDEKSSSSKDGDPQLEFKVLQGYRDLESEMHKGRAQVTRTGDIGVAMDNL 81
Query: 65 DEIEHLFGDLQSLNVNKNAVLAQDSKAMKTISELLSLSMSTVKFDESGRLIKLEDILNSA 124
+ ++ LF + + + N + A D++AM +ISEL +S+ +KFD+S ++ LE+I+NS
Sbjct: 82 NAVDSLFNKV--IGIKNNGLFAHDARAMVSISELAQISVRNLKFDDSRSMVNLENIVNSL 139
Query: 125 KKFMLKEYLEVTGMTXXXXXXXXXXXXLADETHEPVNDFGDKRTSEVDNDD--VSAFKTR 182
K++MLKE+ ++ + + + +D RT + ++ D S+FK
Sbjct: 140 KRYMLKEHFKLNNIAENRNDLTLAADEQSAADQQEESDGDIDRTPDDNHTDKATSSFKAT 199
Query: 183 QKRSNYLSQFGNYTEFNQFNWFKMGTLYQNLSLTPFTIDHMLGPLYVEKTVR--KQREIR 240
R +YL QF +Y EF+QFNWF++G LY +S DH++GPL +EK R QR R
Sbjct: 200 SMRHSYLQQFSHYNEFSQFNWFRIGALYNTISKNAPITDHLMGPLSIEKKPRVLTQRR-R 258
Query: 241 ARDPVAELSTAERVTKESLNESQDETTPSHVRKCYKVLKEKEG-EQQINLFKYIIDPNSF 299
D V E TAE++T+ SLN +Q ETTP V+KC+K L +K G E INLFK+IIDPNSF
Sbjct: 259 NNDQVGEKITAEKITQHSLNSTQQETTPEQVKKCFKKLSKKLGPEGSINLFKFIIDPNSF 318
Query: 300 SKSVENLFYTSFLIKEGRLVLEDDPDGYPAVRIKEQLP-DDSREREIEKQRRNDSRQNHI 358
S+S+ENLFYTSFLIKEG+L++E D +G P ++IK+ + DSR +EIE+QRR + QNHI
Sbjct: 319 SRSIENLFYTSFLIKEGKLLMEHDEEGLPTIKIKQSISHTDSRSKEIERQRRRAAHQNHI 378
Query: 359 IFQMDIPTWRKLIKKFGITEPFLN 382
IFQMD+PTWRKLIKK+ IT PFL+
Sbjct: 379 IFQMDMPTWRKLIKKYNITSPFLD 402
>Hs8923009 [S] KOG2866 Uncharacterized conserved protein
Length = 385
Score = 74.3 bits (181), Expect = 3e-13
Identities = 50/184 (27%), Positives = 84/184 (45%), Gaps = 10/184 (5%)
Query: 202 NWFKMGTLYQNLSLTPFTIDHMLGPLYVEKTVRKQREIRARD-PVAELSTAERVTKESLN 260
+W G +N T +LG +Y E V K R R R PV + A +
Sbjct: 197 SWKITGRTAENTFNKTHTFHFLLGSIYGECPVPKPRVDRPRKVPVIQEERAMPAQLRRME 256
Query: 261 ESQDETTPSHVRKCYKVLK---EKEGEQQINLFKYIIDPNSFSKSVENLFYTSFLIKEGR 317
ES E T V + +L+ ++ + ++ F +++DP+SF ++VEN+F+ SF+I++G
Sbjct: 257 ESHQEATEKEVERILGLLQTYFREDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGF 316
Query: 318 LVLEDDPDGYPAVRIKEQLPDDSREREIEKQRRNDSRQNHIIFQMDIPTWRKLIKKFGIT 377
+ D D P + S E E N +N I + W +++K F I+
Sbjct: 317 ARIRLDQDRLPVIE------PVSINEENEGFEHNTQVRNQGIIALSYRDWEEIVKTFEIS 370
Query: 378 EPFL 381
EP +
Sbjct: 371 EPVI 374
>7297703 [S] KOG2866 Uncharacterized conserved protein
Length = 269
Score = 60.8 bits (146), Expect = 3e-09
Identities = 44/168 (26%), Positives = 77/168 (45%), Gaps = 15/168 (8%)
Query: 218 FTIDHMLGPLYVEKTVRKQREIRARDPVAELSTAERVTKESLNESQDETTPS--HVRKCY 275
FT D ML + V V Q++ R +++ +R K E +DE S H+ K
Sbjct: 109 FTNDSMLPFIDVTPKVVVQKQRAPRKTKSQVDE-KRPEKSDQLERKDEGAASVTHMLKQI 167
Query: 276 KVLKEKEGEQQINLFKYIIDPNSFSKSVENLFYTSFLIKEGRLVLEDDPDGYPAVRIKEQ 335
+ + ++ I FK I +PN+F +V+N SFL+KE + +E+ DG P VR+
Sbjct: 168 RQIYRDGNQEPIPYFKLICNPNNFMDTVQNALQLSFLVKENYISIENGEDGLPLVRV--- 224
Query: 336 LPDDSREREIEKQRRNDSRQNHIIFQMDIPTWRKLIKKFGITEPFLNK 383
+ + + + I +D+ K++K + + EP L +
Sbjct: 225 ---------VNSKSVEGNAPSQAICSIDVTFCEKMVKHYNLHEPMLKR 263
>CE18820 [S] KOG2866 Uncharacterized conserved protein
Length = 459
Score = 57.8 bits (138), Expect = 3e-08
Identities = 43/171 (25%), Positives = 82/171 (47%), Gaps = 18/171 (10%)
Query: 217 PFTI--DHMLGPLYVEKTVRKQREIRAR--------DPVAELSTAERVTKESLNESQDET 266
PF I D ++ + VE+ V KQ++IR + +P A + +++ E
Sbjct: 290 PFNIGCDFIVQLVDVER-VEKQKKIREKVERGAKRKEPAALVKVSDKARNVQSEEVSISK 348
Query: 267 TPSHVRKCYKVLKEKEGEQQINLFKYIIDPNSFSKSVENLFYTSFLIKEGRLVLEDDPDG 326
HV K K + QI+ ++ I +P SFS++VEN+FY S+L+++ + L ++ +G
Sbjct: 349 ELQHVLKVLKKELKTRKTDQIDYYELITNPTSFSRTVENMFYVSYLMRDREVYLLEE-NG 407
Query: 327 YPAVRIKEQLPDDSREREIEKQRRNDSRQNHIIFQMDIPTWRKLIKKFGIT 377
P ++ + D EK + ++ H + + W+ L +K+ T
Sbjct: 408 VPILKKPTAVNDQ------EKLQNDERNSTHGLTPLSFEQWKTLSQKYKTT 452
>SPBC20F10.04c [S] KOG2866 Uncharacterized conserved protein
Length = 113
Score = 57.0 bits (136), Expect = 5e-08
Identities = 35/107 (32%), Positives = 58/107 (53%), Gaps = 9/107 (8%)
Query: 223 MLGPLYVEKTVRKQREIRARDPVAE---LSTAERVTKESLNESQDETTPSHVRKCYKVLK 279
M+GPL K K+R I+ R+ + + + T + E +Q+ T +V ++L
Sbjct: 1 MVGPLSFRK---KERNIQRRERLQKAPNVLTQPTMLNERNITTQENNTTKNVLHISRLL- 56
Query: 280 EKEGEQQINLFKYIIDPNSFSKSVENLFYTSFLIKEGRLVLEDDPDG 326
+ Q +N K+I +P S+ ++VENLFY SFL KEG+ L ++ G
Sbjct: 57 --QAHQPVNFLKFITNPQSYPQTVENLFYVSFLFKEGKAALVENESG 101
>At1g51130 [S] KOG2866 Uncharacterized conserved protein
Length = 403
Score = 53.5 bits (127), Expect = 5e-07
Identities = 44/179 (24%), Positives = 78/179 (42%), Gaps = 12/179 (6%)
Query: 203 WFKMGTLYQNLSLTPFTIDHMLGPLYVEKTVRKQREIRARDPVAELSTAERVTKESLNES 262
W +G + L MLGP+ E RK+ R R E + V E
Sbjct: 166 WKDLGFAVCSTVLVSCGCSTMLGPMDTELKQRKRAPNRKRTKPGEGVRPDEVDDSQSEEK 225
Query: 263 QDETTPSHVRKCYKVLKEKEGEQQINLFKYIIDPNSFSKSVENLFYTSFLIKEGRLVLED 322
D T ++ + +L +K+ Q NL +++ SF+++VENLF SFL K+GR+ +
Sbjct: 226 TD--TDKNMTIMFNILGKKKRVQLENL---VLNRRSFAQTVENLFALSFLAKDGRVEIIV 280
Query: 323 DPDGYPAVRIKEQLPDDSREREIEKQRRNDSRQNHIIFQMDIPTWRKLIKKFGITEPFL 381
D G +P ++ + + + NH +F++D W+ + + + E +
Sbjct: 281 DKSGSHFA-----MPRNAPDANVVMS--GEVIYNHFVFRLDFKDWKLMSEMVPLGEELM 332
>ECU08g1820 [S] KOG2866 Uncharacterized conserved protein
Length = 261
Score = 53.1 bits (126), Expect = 7e-07
Identities = 34/148 (22%), Positives = 71/148 (47%), Gaps = 22/148 (14%)
Query: 234 RKQREIRARDPVAELSTAERVTKESLNESQDETTPSHVRKCYKVLKEKEGEQQINLFKYI 293
R+ + R+R + E +T+E +S +E P + + +++ EK + F+++
Sbjct: 134 RRAQSQRSRQTLPETEVPTLITREK--DSVEE--PEILAQIKRLVAEKG---RTEYFRFV 186
Query: 294 IDPNSFSKSVENLFYTSFLIKEGRLVLEDDPDGYPAVRIKEQLPDDSREREIEKQRRNDS 353
IDP SFSK++EN+FY S ++ G LE D DG + + +
Sbjct: 187 IDPRSFSKTIENIFYLSLSLRSGVAFLEFDDDG---------------TLYVVSEGSEGA 231
Query: 354 RQNHIIFQMDIPTWRKLIKKFGITEPFL 381
H++ +M + +++++ GI++ +
Sbjct: 232 STGHLVVEMTYDEYLRIVERMGISKAMI 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.366
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,381,181
Number of Sequences: 60738
Number of extensions: 892029
Number of successful extensions: 3675
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 3665
Number of HSP's gapped (non-prelim): 7
length of query: 385
length of database: 30,389,216
effective HSP length: 108
effective length of query: 277
effective length of database: 23,829,512
effective search space: 6600774824
effective search space used: 6600774824
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)