ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2493 good R KOG1477 General function prediction only SPRY domain-containing proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2493 852683 851286 -466
(466 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR171c [R] KOG1477 SPRY domain-containing proteins 483 e-136
SPCC285.10c [R] KOG1477 SPRY domain-containing proteins 164 3e-40
YKL124w [R] KOG1477 SPRY domain-containing proteins 118 2e-26
CE20318 [R] KOG1477 SPRY domain-containing proteins 56 1e-07
SPCC1259.12c [R] KOG1477 SPRY domain-containing proteins 55 2e-07
Hs14776143 [R] KOG1477 SPRY domain-containing proteins 52 1e-06
Hs4885571 [R] KOG1477 SPRY domain-containing proteins 52 2e-06
>YMR171c [R] KOG1477 SPRY domain-containing proteins
Length = 550
Score = 483 bits (1244), Expect = e-136
Identities = 249/479 (51%), Positives = 327/479 (67%), Gaps = 41/479 (8%)
Query: 26 VSEGPSDPD-VDMDLIVFTMMFMFLSYLLSILVYFVTRWLVKRLFTARISLHEEGTMNGG 84
VS G D D ++MD+++F + + Y++ +VYF T+ + RL TA + H G
Sbjct: 32 VSYGGDDTDELNMDIMLFAFGTLIVVYIVICIVYFFTKQIATRLITAYYNEHGPGQRISL 91
Query: 85 QSDLSEDRY------------------LDDESEVKEFLQNLSPKEQFYYKQGEEYVKQNP 126
SD E+ LDD EV++ L LSP+EQFYYKQGEEY+KQNP
Sbjct: 92 FSDYDENNAHVHSRRLMENMSLRWPNNLDDADEVRDKLAQLSPEEQFYYKQGEEYIKQNP 151
Query: 127 PLLVPY---LPNDPENREEVIPDPIVNEQTLQFIEEEGAAAWEFQADPNLPNDTVMIQER 183
P L+ ++ N + DPI+NEQT Q+I+EEGA AWEF +P++PN TV+++ +
Sbjct: 152 PFLLNQGLLQQSEDSNPDTTREDPIMNEQTRQYIQEEGAYAWEFSPNPDMPNHTVIVENK 211
Query: 184 TELTFLNFSYDASVMTTLPIPRLNKVYYYECKIFE----LNQGKNHLSDNEMISIGLSTS 239
TE++FLN++YDAS+ T LPIP +NKVYY E KIFE LN +N +IS GLST
Sbjct: 212 TEVSFLNYNYDASISTNLPIPCINKVYYCEFKIFETDGPLNSDEN--VSKGVISFGLSTQ 269
Query: 240 PYPYFRLPGRHHHSISYDSDGCRRMNSSFPMSAELQTLFPRFEKGDVIGIGYRTRSGTIF 299
PYPYFRLPGRHHHSI+YDS+G RR N SF ++ +L+TLFP+ EKGD++GIGYR+RSGT+F
Sbjct: 270 PYPYFRLPGRHHHSIAYDSNGARRFNDSFKLNEQLRTLFPQCEKGDIVGIGYRSRSGTVF 329
Query: 300 FTHNGKKLNEKKVGGHIKGWKLKYLYPIVGSNIPCKIHVNFGTYGFVYIEANVKKWGYSK 359
FT NGKKLNEK VGGHI+GWK +YLYPI+GSN+PC+IHVNFGTYGFVYIEANVKKWGY+K
Sbjct: 330 FTRNGKKLNEKSVGGHIRGWKFQYLYPIIGSNVPCQIHVNFGTYGFVYIEANVKKWGYAK 389
Query: 360 NAGIKLPPPSYEDYDQDVLLESA-----FEDDVSDSESI-----TSDSINEQITDQSGNI 409
+ GIKLPPPSYEDY +D LLES F++D SD +S ++ ++N+ I ++G I
Sbjct: 390 SNGIKLPPPSYEDYGKDTLLESGGEDNDFDEDFSDGDSDNIAAGSTTNLNDDIIIRNGEI 449
Query: 410 LPPPPGFEFSTS--VASDIPVESITMHSLPAEPPNYSADEDEEHNVEDNTIANQFVATT 466
LPPPPGFEF+ S I E I + SLP PP+YS DE N + + I+ + + T+
Sbjct: 450 LPPPPGFEFTMSPPTGKKIINEEINLDSLPMLPPSYSDDEHHSKN-DKSAISGRIIGTS 507
>SPCC285.10c [R] KOG1477 SPRY domain-containing proteins
Length = 382
Score = 164 bits (414), Expect = 3e-40
Identities = 104/293 (35%), Positives = 160/293 (54%), Gaps = 43/293 (14%)
Query: 157 IEEEGAAAWEFQADPNLPND--TVMIQERTELTFLNFSYDASVMTTLPIPRLNKVYYYEC 214
++E+G AW F +PN ++ +TEL+FL + + + T LP+ R+N+VYY+E
Sbjct: 118 VQEKGVLAWHF-----IPNQEYNCYVKNKTELSFLG-NEECCMQTNLPLQRINEVYYFEV 171
Query: 215 KIFELNQGKNHLSDNEMISIGLSTSPYPYFRLPGRHHHSISYDSDGCRRMNSSFPMSAEL 274
K+ ++ + ++SIGL+T PYP FRLPG + S +Y SDG RR NS P + +
Sbjct: 172 KLLDV-------PIDTLVSIGLATKPYPPFRLPGWNFWSTAYVSDGTRRSNS--PFTGKP 222
Query: 275 QTLFPRFEKGDVIGIGYRTRSGTIFFTHNGKKLNEKKVGGHIKGWKLKYLYPIVGSNIPC 334
+ F +++GDVIG+GY+ + IFFT NG++ E + LYP VG+ PC
Sbjct: 223 YSSF--YQQGDVIGVGYKPKCNRIFFTRNGRRCAELPC-------TYRNLYPTVGAIGPC 273
Query: 335 KIHVNFGTYGFVYIEANVKKWGYSKNAGIKLPPPSYEDYDQDVLLESAFEDDVSDSESIT 394
+HVN G G+V+IEAN+KKW + G PPPSY + ++A S+S + T
Sbjct: 274 TLHVNLGQAGYVFIEANIKKWRLAPAVGSLAPPPSYSTSQPTISWDAA-----SESSAGT 328
Query: 395 SDSINEQITDQSGNILPP---------PPGFEFSTSVASDIPVESITMHSLPA 438
+ + D+S N PP G FS S +++ ++ MHS+PA
Sbjct: 329 TTQGDTNRPDKSKNRSPPINFDGTSYDAAGNVFSPSSSNN---QAYQMHSMPA 378
>YKL124w [R] KOG1477 SPRY domain-containing proteins
Length = 579
Score = 118 bits (296), Expect = 2e-26
Identities = 90/321 (28%), Positives = 144/321 (44%), Gaps = 56/321 (17%)
Query: 90 EDRYLDDESEVKEFLQNLSPKEQFYYKQGEEYVKQNPPLLVP---YLPNDPENREEVIPD 146
+D + E E++E L +S E Y + +E+ +PP++ YL +D +
Sbjct: 117 DDEIVLQERELEE-LPKMSAYEVELYIRAKEFQMMSPPMVKDFGTYLDSDDQ-------- 167
Query: 147 PIVNEQTLQFIEEEGAAAWEFQADPNLPND-------TVMIQERTELTFLNFSYDASVMT 199
QFI++ G ++ N D + ++Q++ ++ F F+ +S +
Sbjct: 168 --------QFIKDRGIQSYFLLPSINDNIDEYGNFLPSFIVQDKLDIQFSKFNKSSSTVM 219
Query: 200 TLPIPRLNK-VYYYECKIFELNQGKNHLSDNEMISIGLSTSPYPYFRLPGRHHHSISYDS 258
P+P K Y+E KIF Q N + SIGL+T PYPYFR+PG +SI+Y+S
Sbjct: 220 NYPLPHNRKDAVYFEVKIFRHIQ-----KSNSIFSIGLTTVPYPYFRVPGMAKYSIAYES 274
Query: 259 DGCRRMNSSFPMSAELQTLFPRFEKGDVIGIGYRTRSGTIFFTHNGKKLNEKKVGGHIKG 318
G R+N+ F S TL P+ E+GD +G GYR ++GTIF THNGKKL +
Sbjct: 275 TGKLRINNPFTAS----TLLPKLEEGDTVGFGYRYKTGTIFITHNGKKLMD--------- 321
Query: 319 WKLKYLYPIVGSNIPCKIHVNFGTYGFVYIEANVKKWGYSKNAGIKLPPPSYEDYDQDVL 378
V NI + + G + Y + + E D +V
Sbjct: 322 ---------VTQNIGIDLFIGIGAFNAAYTRTYTRDGLLEDPDNVSFREALSEGKDIEVA 372
Query: 379 LE-SAFEDDVSDSESITSDSI 398
+ D +S+ +TSD +
Sbjct: 373 KDLQRVHDPHDESDEMTSDEV 393
>CE20318 [R] KOG1477 SPRY domain-containing proteins
Length = 622
Score = 56.2 bits (134), Expect = 1e-07
Identities = 48/140 (34%), Positives = 58/140 (41%), Gaps = 17/140 (12%)
Query: 209 VYYYECKIFELNQGKNHLSDNEMISIGLSTSPYPYFRLPGRHHHSISYDSDGCRRMNSSF 268
VYY+E +I E + G + IGLS R+PG Y D + F
Sbjct: 95 VYYFEVRILEGHSG--------CMGIGLSKKDCDLNRMPGWDPGCFGYHGDD----GNFF 142
Query: 269 PMSAELQTLFPRFEKGDVIGIGYRTRSGTIFFTHNGKKLNEKKVGGHIKGWKLKYLYPIV 328
S Q P+F GDVIG G T +FFT NGK L H LK LYP V
Sbjct: 143 LASGVGQAYGPKFGPGDVIGCGIDTFYHYVFFTKNGKHLGI----AHTSPTALKELYPTV 198
Query: 329 GSNIPC-KIHVNFGTYGFVY 347
G K+ NFG F +
Sbjct: 199 GFKTRGEKLVANFGVKPFEF 218
>SPCC1259.12c [R] KOG1477 SPRY domain-containing proteins
Length = 486
Score = 55.1 bits (131), Expect = 2e-07
Identities = 50/156 (32%), Positives = 66/156 (42%), Gaps = 22/156 (14%)
Query: 195 ASVMTTLPIPRLNKVYYYECKIFELNQGKNHLSDNEMISIGLSTSPYPYFRLPGRHHHSI 254
ASV IP +YYYE +I L++GK + +G RLPG S
Sbjct: 99 ASVKADHAIPSNTSIYYYEIQI--LSRGKEG-----KMGVGFCRKSMQTNRLPGCTAESW 151
Query: 255 SYDSDGCRRMNSSFPMSAELQTLFPRFEKGDVIGIGYRTRSGTIFFTHNGKKLNE--KKV 312
Y + + N S A P F GD+IG G + TIF+T NG L KKV
Sbjct: 152 GYHGNSGEKFNCSKTGEA----YGPEFTTGDIIGCGVNFINRTIFYTKNGAYLGVAFKKV 207
Query: 313 GGHIKGWKLKYLYPIVGSNIPCK-IHVNFGTYGFVY 347
LYP++G + + VNFG F+Y
Sbjct: 208 S--------DVLYPVIGLKSHGEHVEVNFGQNPFLY 235
>Hs14776143 [R] KOG1477 SPRY domain-containing proteins
Length = 503
Score = 52.4 bits (124), Expect = 1e-06
Identities = 45/126 (35%), Positives = 54/126 (42%), Gaps = 12/126 (9%)
Query: 232 ISIGLSTSPYPYFRLPGRHHHSISYDSDGCRRMNSSFPMSAELQTLFPRFEKGDVIGIGY 291
+ IGLS RLPG HS Y D SF S Q P F GDVIG
Sbjct: 1 MGIGLSAQGVNMNRLPGWDKHSYGYHGDD----GHSFCSSGTGQPYGPTFTTGDVIGCCV 56
Query: 292 RTRSGTIFFTHNGKKLNEKKVGGHIKGWKLKYLYPIVGSNIPCKI-HVNFGTYGFVY-IE 349
+GT F+T NG L G LYP VG P +I NFG F++ IE
Sbjct: 57 NLINGTCFYTKNGHSL------GIAFTDLPANLYPTVGLQTPGEIVDANFGQQPFLFDIE 110
Query: 350 ANVKKW 355
+++W
Sbjct: 111 DYMREW 116
>Hs4885571 [R] KOG1477 SPRY domain-containing proteins
Length = 500
Score = 52.0 bits (123), Expect = 2e-06
Identities = 44/126 (34%), Positives = 54/126 (41%), Gaps = 12/126 (9%)
Query: 232 ISIGLSTSPYPYFRLPGRHHHSISYDSDGCRRMNSSFPMSAELQTLFPRFEKGDVIGIGY 291
+ IGLS RLPG HS Y D SF S Q P F GDVIG
Sbjct: 1 MGIGLSAQGVNMNRLPGWDKHSYGYHGDD----GHSFCSSGTGQPYGPTFTTGDVIGCCV 56
Query: 292 RTRSGTIFFTHNGKKLNEKKVGGHIKGWKLKYLYPIVGSNIPCK-IHVNFGTYGFVY-IE 349
+ T F+T NG L G LYP VG P + + NFG + FV+ IE
Sbjct: 57 NLINNTCFYTKNGHSL------GIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIE 110
Query: 350 ANVKKW 355
+++W
Sbjct: 111 DYMREW 116
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.136 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,672,913
Number of Sequences: 60738
Number of extensions: 1478887
Number of successful extensions: 4705
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4689
Number of HSP's gapped (non-prelim): 8
length of query: 466
length of database: 30,389,216
effective HSP length: 110
effective length of query: 356
effective length of database: 23,708,036
effective search space: 8440060816
effective search space used: 8440060816
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)