ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2541 good OU KOG2608 Intracellular trafficking, secretion, and vesicular transport Endoplasmic reticulum membrane-associated oxidoreductin involved in disulfide bond formation
r_klactIV2541 good OU KOG2608 Posttranslational modification, protein turnover, chaperones Endoplasmic reticulum membrane-associated oxidoreductin involved in disulfide bond formation
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2541 867365 865683 -561
(561 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML130c [OU] KOG2608 Endoplasmic reticulum membrane-associated o... 637 0.0
SPCC1450.14c [OU] KOG2608 Endoplasmic reticulum membrane-associa... 245 1e-64
SPBC4F6.16c [OU] KOG2608 Endoplasmic reticulum membrane-associat... 228 2e-59
At2g38960 [OU] KOG2608 Endoplasmic reticulum membrane-associated... 210 5e-54
Hs7657069 [OU] KOG2608 Endoplasmic reticulum membrane-associated... 208 2e-53
Hs9845249 [OU] KOG2608 Endoplasmic reticulum membrane-associated... 204 2e-52
7292447 [OU] KOG2608 Endoplasmic reticulum membrane-associated o... 203 6e-52
At1g72280 [OU] KOG2608 Endoplasmic reticulum membrane-associated... 202 1e-51
CE28787 [OU] KOG2608 Endoplasmic reticulum membrane-associated o... 175 1e-43
ECU04g0170 [OU] KOG2608 Endoplasmic reticulum membrane-associate... 56 2e-07
>YML130c [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 563
Score = 637 bits (1643), Expect = 0.0
Identities = 302/531 (56%), Positives = 400/531 (74%), Gaps = 14/531 (2%)
Query: 40 DSTNFCRMDKTEAIGATCDVTFHEINEVNNNIRPQLLSLVQSDFFKYFKLDLYKECPFWS 99
+ T+FC++D+ + + +C+VTF+E+N +N NIR L +L++SDFFKYF+LDLYK+C FW
Sbjct: 35 NDTHFCKVDRNDHVSPSCNVTFNELNAINENIRDDLSALLKSDFFKYFRLDLYKQCSFWD 94
Query: 100 DNNGYCVNRACAVDVVEDWDSLPEYWQPEILGNIENAT---TDITDDECSFLDELCD--K 154
N+G C+NRAC+VDVVEDWD+LPEYWQPEILG+ N T D +DDEC FLD+LC K
Sbjct: 95 ANDGLCLNRACSVDVVEDWDTLPEYWQPEILGSFNNDTMKEADDSDDECKFLDQLCQTSK 154
Query: 155 PRFEAEKDIEYCDTNDFNSQHSVLVDLTANPERFTGYGGEQSSQIWSSIYKENCFSLGDE 214
+ E I YCD NDFN +++VL+DLTANPERFTGYGG+Q+ QIWS+IY++NCF++G+
Sbjct: 155 KPVDIEDTINYCDVNDFNGKNAVLIDLTANPERFTGYGGKQAGQIWSTIYQDNCFTIGET 214
Query: 215 NQCLAKDAFYRLISGLHASIGTHLSNEHLNTETGKWEPNLELFMTRVGNFPDRVSNIYFN 274
+ LAKDAFYRL+SG HASIGTHLS E+LNT+TGKWEPNL+LFM R+GNFPDRV+N+YFN
Sbjct: 215 GESLAKDAFYRLVSGFHASIGTHLSKEYLNTKTGKWEPNLDLFMARIGNFPDRVTNMYFN 274
Query: 275 FAVVAKALWKIRPYMNELGFCNAYNEDVKGMIDGVVSQLNSKVFNEDLLFHDEVSGQLKD 334
+AVVAKALWKI+PY+ E FC+ N+++K +D V+SQL++K+FNEDL+F +++S LKD
Sbjct: 275 YAVVAKALWKIQPYLPEFSFCDLVNKEIKNKMDNVISQLDTKIFNEDLVFANDLSLTLKD 334
Query: 335 DFRIRFKNVTKIMDCVQCDRCRLWGKVQTTGYATSLKILFEMDNDDDVARQHVVDKLTKY 394
+FR RFKNVTKIMDCVQCDRCRLWGK+QTTGYAT+LKILFE+++ D+ +QH+V KLTKY
Sbjct: 335 EFRSRFKNVTKIMDCVQCDRCRLWGKIQTTGYATALKILFEINDADEFTKQHIVGKLTKY 394
Query: 395 ELIALFNTFDRLSKSVEAVNRFEEMYNYQLKSPGEKLASFFQLDNFFKVLNDKFNSANHS 454
ELIAL TF RLS+S+E+VN FE+MY +L +L+SFFQ +NFF +L + S ++
Sbjct: 395 ELIALLQTFGRLSESIESVNMFEKMYGKRLNGSENRLSSFFQ-NNFFNILKEAGKSIRYT 453
Query: 455 SQEKESVSNVEKKVNE-----FNDLKMPEKKKETHTEVREQASGVFKEAWDVEWKNFKQA 509
+ S +KK N F+DLKMP K E +K+AW+ E N +A
Sbjct: 454 IENINSTKEGKKKTNNSQSHVFDDLKMP--KAEIVPRPSNGTVNKWKKAWNTEVNNVLEA 511
Query: 510 LRFIVTSYTDLPSTVSKYTVLKLNKLWNKFIGVPNYLEEGEELEYPSYNYE 560
RFI SY DLP + + +++K+ K WNKFIGV +Y+ E E E SY +
Sbjct: 512 FRFIYRSYLDLPRNIWELSLMKVYKFWNKFIGVADYVSE-ETREPISYKLD 561
>SPCC1450.14c [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 571
Score = 245 bits (626), Expect = 1e-64
Identities = 148/430 (34%), Positives = 241/430 (55%), Gaps = 54/430 (12%)
Query: 48 DKTEAIGATCDVTFHEINEVNNNIRPQLLSLVQ-SDFFKYFKLDLY-KECPFWSDNNGYC 105
D I T + ++ +I +N+ + P L L + SD+ ++++L+L+ KEC + D+N C
Sbjct: 43 DGKSIIHDTLNSSYSDIENLNSRVSPLLFDLTEKSDYMRFYRLNLFNKECRYNLDDNVAC 102
Query: 106 VNRACAVDVVEDWDSLPEYWQPEILGNIENATTDI------TD----DECSFLDELCDKP 155
+ AC V V ++ D +PE W + LG +E ++ TD + + + C
Sbjct: 103 GSSACNVLVTDEQD-VPEVWSSKSLGKLEGFMPELSRQIVETDRSVMEHVDKISQSCLLE 161
Query: 156 RFEAEKDIEYCDTNDFNSQHSVLVDLTANPERFTGYGGEQSSQIWSSIY----------- 204
R + E +YC ++ V V L NPERFTGY G S++IW IY
Sbjct: 162 RLDDEAH-QYCYVDNELDSGCVYVSLLENPERFTGYSGPHSTRIWEMIYNQCLPDSSAPT 220
Query: 205 ------------------KENCFSLGDENQCLAKDAFYRLISGLHASIGTHLSNEHLNTE 246
+E + L + FYR++SG+H+SI THL + +LN
Sbjct: 221 IDFPALFMQGPLAPPPKPQEQLLKERMDAWTLEQRVFYRVLSGMHSSISTHLCHGYLNQR 280
Query: 247 TGKWEPNLELFMTRVGNFPDRVSNIYFNFAVVAKALWKIRPYMNELGFCN---AYNEDVK 303
G W PNL+ F +V N+P+R+ N+YF +A++ +A+ K+ +++ L FC+ + +V+
Sbjct: 281 NGVWGPNLQCFQEKVLNYPERLENLYFAYALMQRAIDKLYGHLDSLTFCHDSVLQDSEVR 340
Query: 304 GMIDGVVSQLNS--KVFNEDLLFHDE--VSGQLKDDFRIRFKNVTKIMDCVQCDRCRLWG 359
I G+VS +++ K+F+E +LF + +S LK+DFR FK V+ +MDCV C+RCRLWG
Sbjct: 341 QKIAGLVSLIHNSPKMFDETMLFAGDPSISTALKEDFREHFKTVSALMDCVGCERCRLWG 400
Query: 360 KVQTTGYATSLKILFEMDN-DDDVAR---QHVVDKLTKYELIALFNTFDRLSKSVEAVNR 415
K+QT G+ T+LKILFE+ N +D+V + +L + E++AL NTFDRLS+S+ V+
Sbjct: 401 KIQTNGFGTALKILFEVSNIEDEVTNFDSRSFSLRLRRSEIVALINTFDRLSRSINFVDD 460
Query: 416 FEEMYNYQLK 425
F+++Y+ Q K
Sbjct: 461 FKQIYSEQHK 470
>SPBC4F6.16c [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 467
Score = 228 bits (580), Expect = 2e-59
Identities = 141/447 (31%), Positives = 229/447 (50%), Gaps = 84/447 (18%)
Query: 51 EAIGATCDVTFHEINEVNNNIRPQLLSLVQ-SDFFKYFKLDLYKE-CPFWSDNNGYCVNR 108
E I + D + I ++ + +R + L + SD+F Y++++LY+ CP W ++N C N+
Sbjct: 22 EIIISQTDTSSTGIYQMQSKVRSLVPVLTERSDYFSYYRVNLYRSSCPLWENDNAMCSNQ 81
Query: 109 ACAVDVVEDWDSLPEYWQPEILGNIENATTDITDDECSFLDELCDKPRFEAEKDIEYCDT 168
CAV + + + +P+ W+ L + E + D +C++ + D++YC
Sbjct: 82 GCAVKSLNEIE-IPKVWKK--LSDFEPHSKK-NDTKCNW----------KYNPDLDYCYL 127
Query: 169 NDFNSQHS-VLVDLTANPERFTGYGGEQSSQIWSSIYKENCFSLGDENQ----------- 216
++ S V V L NPERFTGY G+ S+ IW SIY++NCF + D++
Sbjct: 128 DNSTSPDEYVYVSLVQNPERFTGYAGDHSAAIWRSIYEQNCFVVDDDDNPSEQPKSNALF 187
Query: 217 ------------------------CLAKDAFYRLISGLHASIGTHLSNEHLNTETGKWEP 252
CL K F R+ISG HASI TH+ + + E +W
Sbjct: 188 RPNQIPLNLFTENHDDTSLSPSVACLEKRMFNRIISGFHASISTHVCQNYYDVEEQRWTQ 247
Query: 253 NLELFMTRVGNFPDRVSNIYFNFAVVAKALWKIRPYMNELGFCNAY-----NEDVKGMID 307
NL+ + +VG+FPDR+ NI+FN+A++ +AL +I M + +++ ++DV
Sbjct: 248 NLDWWRAKVGSFPDRIENIFFNYALLHQALVQIATQMKNITSDSSFTFCPTDKDVDWRTH 307
Query: 308 GVVSQL------NSKVFNEDLLFHDEVSGQLKDDFRIRFKNVTKIMDCVQCDRCRLWGKV 361
QL + V N + F DE + + KD FR F+++++IMDCV CD+CRLWGKV
Sbjct: 308 TAFEQLVYHAYQDRHVINREQFFADEEAKRFKDSFRKHFRDISRIMDCVGCDKCRLWGKV 367
Query: 362 QTTGYATSLKILFEMDNDDDVARQHVVDKLTKYELIALFNTFDRLSKSVEA--------- 412
Q TGY T+LK+L E + + L E++AL NTFDR+S SVEA
Sbjct: 368 QITGYGTALKLLLEPETQ--------LSDLRPEEVVALVNTFDRISHSVEATFWFSLKAK 419
Query: 413 ----VNRFEEMYNYQLKSPGEKLASFF 435
++ + + +SP +++ +FF
Sbjct: 420 TNDTLDAIKALIYKTYRSPSKEILAFF 446
>At2g38960 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 519
Score = 210 bits (534), Expect = 5e-54
Identities = 130/374 (34%), Positives = 201/374 (52%), Gaps = 28/374 (7%)
Query: 57 CDVTFHEINEVNNNI-RPQLLSLVQSDFFKYFKLDLYKECPFWSDNNGYCVNRACAV--- 112
C + +N +N + P L LV++ F++YFK+ L+ +CPFW D+ G C R C+V
Sbjct: 72 CCCDYETVNRLNTEVLNPLLQDLVKTPFYRYFKVKLWCDCPFWPDD-GMCRLRDCSVCEC 130
Query: 113 ---DVVEDWDSLPEYWQPEILGNIENATTDITDDECSFLDELCDKPRFEAEKDIEYCDTN 169
+ E + + P AT D T D +F R D + +
Sbjct: 131 PESEFPEVFKKPLSQYNPVCQEGKPQATVDRTLDTRAF--------RGWTVTDNPWTSDD 182
Query: 170 DFNSQHSVLVDLTANPERFTGYGGEQSSQIWSSIYKENCFSLGDENQCLAKDAFYRLISG 229
+ ++ V+L NPER+TGY G + +IW +IY ENC E C + Y+L+SG
Sbjct: 183 ETDNDEMTYVNLRLNPERYTGYIGPSARRIWEAIYSENCPKHTSEGSCQEEKILYKLVSG 242
Query: 230 LHASIGTHLSNEHLNTE-TGKWEPNLELFMTRVGNFPDRVSNIYFNFAVVAKALWKIRPY 288
LH+SI H+++++L E T W NL L RV +PDRV N+YF F V +A+ K Y
Sbjct: 243 LHSSISVHIASDYLLDEATNLWGQNLTLLYDRVLRYPDRVQNLYFTFLFVLRAVTKAEDY 302
Query: 289 MNELGF-CNAYNEDV--KGMIDGVVSQLNSKV-----FNEDLLFHDEVSGQLKDDFRIRF 340
+ E + ED+ K ++ VVS +K F+E L+ + +LK +F
Sbjct: 303 LGEAEYETGNVIEDLKTKSLVKQVVSDPKTKAACPVPFDEAKLWKGQRGPELKQQLEKQF 362
Query: 341 KNVTKIMDCVQCDRCRLWGKVQTTGYATSLKILFEMDNDDDVARQHVVDKLTKYELIALF 400
+N++ IMDCV C++CRLWGK+Q G T+LKILF ++ +D++ +H ++ L + E+IAL
Sbjct: 363 RNISAIMDCVGCEKCRLWGKLQILGLGTALKILFTVNGEDNL--RHNLE-LQRNEVIALM 419
Query: 401 NTFDRLSKSVEAVN 414
N RLS+SV+ V+
Sbjct: 420 NLLHRLSESVKYVH 433
>Hs7657069 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 468
Score = 208 bits (529), Expect = 2e-53
Identities = 142/422 (33%), Positives = 219/422 (51%), Gaps = 63/422 (14%)
Query: 56 TCDVTFHEINEVNN-NIRPQLLSLVQSDFFKYFKLDLYKECPFWSDNNGYCVNRACAVDV 114
TCDV I+ NN + P+L L++SD+F+Y+K++L + CPFW+D + C R CAV
Sbjct: 47 TCDV--ETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDIS-QCGRRDCAVKP 103
Query: 115 VEDWDSLPEYWQPEILGNIENATTDITD-DECSFLDELCDKPRFEAEKDI---------- 163
+ D +P+ + E A I + ++ L + + E +K +
Sbjct: 104 CQS-DEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSS 162
Query: 164 -EYCDTNDFNSQHSVLVDLTANPERFTGYGGEQSSQIWSSIYKENCFS------------ 210
+C+ +D S + VDL NPER+TGY G + +IW+ IY+ENCF
Sbjct: 163 DNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLA 222
Query: 211 ----LGDENQ--------CLAKDAFYRLISGLHASIGTHLSNEHLNTETG---KWEPNLE 255
+EN C+ K AFYRLISGLHASI HLS +L ET KW N+
Sbjct: 223 SGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNIT 282
Query: 256 LFMTRV------GNFPDRVSNIYFNFAVVAKALWKIRPYMNELGFC-----NAYNEDVKG 304
F R G P R+ N+YF + + +AL K+ P+ F +E+ K
Sbjct: 283 EFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKM 342
Query: 305 MIDGVVSQLNSKV--FNEDLLFHDEV--SGQLKDDFRIRFKNVTKIMDCVQCDRCRLWGK 360
++ ++ ++ S F+E+ F + + +LK+DFR+ F+N+++IMDCV C +CRLWGK
Sbjct: 343 LLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGK 402
Query: 361 VQTTGYATSLKILFE---MDNDDDVARQHVVDKLTKYELIALFNTFDRLSKSVEAVNRFE 417
+QT G T+LKILF + N + + LT+ E+++LFN F R+S SV+ + F
Sbjct: 403 LQTQGLGTALKILFSEKLIANMPESGPSYEF-HLTRQEIVSLFNAFGRISTSVKELENFR 461
Query: 418 EM 419
+
Sbjct: 462 NL 463
>Hs9845249 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 467
Score = 204 bits (520), Expect = 2e-52
Identities = 142/444 (31%), Positives = 220/444 (48%), Gaps = 77/444 (17%)
Query: 43 NFCRMDKTEAIGATCDVTFHEINEVNN----NIRPQLLSLVQSDFFKYFKLDLYKECPFW 98
+F R + D +I+ ++N I P++ L + D+F+Y+K++L + CPFW
Sbjct: 25 SFLRSVVEAQVTGVLDDCLCDIDSIDNFNTYKIFPKIKKLQERDYFRYYKVNLKRPCPFW 84
Query: 99 SDNNGYCVNRACAVD---------------------VVEDWDSLPEYWQPEILGNIENAT 137
+++ G+C + C V+ + + L + Q LG I +
Sbjct: 85 AED-GHCSIKDCHVEPCPESKIPVGIKAGHSNKYLKMANNTKELEDCEQANKLGAINSTL 143
Query: 138 TDITDDECSFLDELCDKPRFEAEKDIEYCDTNDFNSQHSVLVDLTANPERFTGYGGEQSS 197
++ + + D R++ +D +C+ +D S + VDL NPER+TGY G +
Sbjct: 144 SNQSKEA------FIDWARYDDSRD-HFCELDDERSPAAQYVDLLLNPERYTGYKGTSAW 196
Query: 198 QIWSSIYKENCF--------------SLGD----------ENQCLAKDAFYRLISGLHAS 233
++W+SIY+ENCF S G+ E CL K FY+LISGLHAS
Sbjct: 197 RVWNSIYEENCFKPRSVYRPLNPLAPSRGEDDGESFYTWLEGLCLEKRVFYKLISGLHAS 256
Query: 234 IGTHLSNEHLNTET-GK--WEPNLELFMTRV------GNFPDRVSNIYFNFAVVAKALWK 284
I HL +L ET GK W PN++ F R G P R+ N+YF + + +AL K
Sbjct: 257 INLHLCANYLLEETWGKPSWGPNIKEFKHRFDPVETKGEGPRRLKNLYFLYLIELRALSK 316
Query: 285 IRPYMN----ELGFCNAYNE-DVKGMIDGVVSQLNS--KVFNEDLLFHDEVSG--QLKDD 335
+ PY +L NA + D K ++ + S F+E +F + G LK++
Sbjct: 317 VAPYFERSIVDLYTGNAEEDADTKTLLLNIFQDTKSFPMHFDEKSMFAGDKKGAKSLKEE 376
Query: 336 FRIRFKNVTKIMDCVQCDRCRLWGKVQTTGYATSLKILFEMDNDDDVARQHVVD--KLTK 393
FR+ FKN+++IMDCV CD+CRLWGK+QT G T+LKILF + +LT+
Sbjct: 377 FRLHFKNISRIMDCVGCDKCRLWGKLQTQGLGTALKILFSEKEIQKLPENSPSKGFQLTR 436
Query: 394 YELIALFNTFDRLSKSVEAVNRFE 417
E++AL N F RLS S+ + F+
Sbjct: 437 QEIVALLNAFGRLSTSIRDLQNFK 460
>7292447 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 416
Score = 203 bits (516), Expect = 6e-52
Identities = 140/415 (33%), Positives = 217/415 (51%), Gaps = 75/415 (18%)
Query: 71 IRPQLLSLVQSDFFKYFKLDLYKECPFWSDNNGYCVNRACAVDVVEDWDSLPE------- 123
I P+L SL+ +FF+++K++L +ECPFW D++ C R C V+ E+ ++P+
Sbjct: 3 IYPRLQSLLVKNFFRFYKVNLRQECPFWPDDSR-CAMRFCQVENCEE-QAIPQGIKDKGE 60
Query: 124 --------YWQPEILGNIENATTDITDDECSFLD-----------ELCDKPRFEAEKDIE 164
Y + +G + + D FLD EL K EAE+D
Sbjct: 61 HKEKAAFKYTREAQVGGSACSDGEDFDSSLGFLDTSISDQAHREFELWAK-HDEAEEDFC 119
Query: 165 YCDTNDFNSQHSVLVDLTANPERFTGYGGEQSSQIWSSIYKENCFSLGDE---------- 214
D ++ SQ+ VDL NPER+TGY GE + +IW SIY ENCF +E
Sbjct: 120 IVDDHEEGSQY---VDLLLNPERYTGYKGESAHRIWKSIYLENCFGGNNETANKFSNYVP 176
Query: 215 -----NQCLAKDAFYRLISGLHASIGTHLSNEHLNTET-------GKWEPNLELFMTRV- 261
N CL + AFYR+ISGLH+SI HL +++L +E+ G W PN++ F R
Sbjct: 177 HLDLRNVCLEQRAFYRIISGLHSSINIHLCSKYLLSESKDFLDPQGIWGPNVKEFKRRFS 236
Query: 262 -----GNFPDRVSNIYFNFAVVAKALWKIRPYMNELGFCNAYNED---VKGMIDGVVSQL 313
G P + N+YF + + +AL K PY+ + E+ VK I+ ++S +
Sbjct: 237 PETTSGEGPHWLRNLYFIYLIELRALAKAAPYLRREDYYTGIAEEDDEVKLAINDMLSVI 296
Query: 314 NS--KVFNEDLLFHDEVSG-QLKDDFRIRFKNVTKIMDCVQCDRCRLWGKVQTTGYATSL 370
+ F+E+ LF + ++ + K D++ +F+N+++IM CV CD+C+LWGK+QT G T+L
Sbjct: 297 ENFQSHFDENALFSNGIASIKFKHDYKEKFRNISRIMSCVGCDKCKLWGKLQTQGLGTAL 356
Query: 371 KILF-EMDN--------DDDVARQHVVDKLTKYELIALFNTFDRLSKSVEAVNRF 416
KIL+ E N D + +L++ E++ALFN F RLS S+ + F
Sbjct: 357 KILYSEKLNLATESGLWDKPHIEADPIFRLSRTEIVALFNAFGRLSNSIYEMENF 411
>At1g72280 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 469
Score = 202 bits (514), Expect = 1e-51
Identities = 128/400 (32%), Positives = 210/400 (52%), Gaps = 23/400 (5%)
Query: 32 DINTVFDNDSTNFCRMDKT---EAIGATCDVTFHEINEVNNNI-RPQLLSLVQSDFFKYF 87
++ F + ++ C + KT + + C + ++ +N + P L LV + FF+Y+
Sbjct: 41 NVGFFFSDRNSCSCSLQKTGKYKGMIEDCCCDYETVDNLNTEVLNPLLQDLVTTPFFRYY 100
Query: 88 KLDLYKECPFWSDNNGYCVNRACAVDVVEDWDSLPEYWQPEILGNIENATTDITDDECSF 147
K+ L+ +CPFW D+ G C R C+V + + + +P + G +
Sbjct: 101 KVKLWCDCPFWPDD-GMCRLRDCSVCECPENEFPEPFKKPFVPGLPSDDLKCQEGKPQGA 159
Query: 148 LDELCDKPRFEA--EKDIEYCDTNDFNSQHSVLVDLTANPERFTGYGGEQSSQIWSSIYK 205
+D D F E + +D +S V+L NPER+TGY G + +IW SIY
Sbjct: 160 VDRTIDNRAFRGWVETKNPWTHDDDTDSGEMSYVNLQLNPERYTGYTGPSARRIWDSIYS 219
Query: 206 ENCFSLGDENQCLAKDAFYRLISGLHASIGTHLSNEHLNTET-GKWEPNLELFMTRVGNF 264
ENC C K Y+LISGLH+SI H++ ++L E+ +W N+EL R+
Sbjct: 220 ENCPKYSSGETCPEKKVLYKLISGLHSSISMHIAADYLLDESRNQWGQNIELMYDRILRH 279
Query: 265 PDRVSNIYFNFAVVAKALWKIRPYMNELGF-CNAYNEDVKGMIDGVVSQL--NSKV---- 317
PDRV N+YF + V +A+ K Y+ + + + ED+K ++ QL + K+
Sbjct: 280 PDRVRNMYFTYLFVLRAVTKATAYLEQAEYDTGNHAEDLK--TQSLIKQLLYSPKLQTAC 337
Query: 318 ---FNEDLLFHDEVSGQLKDDFRIRFKNVTKIMDCVQCDRCRLWGKVQTTGYATSLKILF 374
F+E L+ + +LK + +F+N++ +MDCV C++CRLWGK+Q G T+LKILF
Sbjct: 338 PVPFDEAKLWQGQSGPELKQQIQKQFRNISALMDCVGCEKCRLWGKLQVQGLGTALKILF 397
Query: 375 EMDNDDDVARQHVVDKLTKYELIALFNTFDRLSKSVEAVN 414
+ N D+ Q + +L + E+IAL N +RLS+SV+ V+
Sbjct: 398 SVGN-QDIGDQTL--QLQRNEVIALVNLLNRLSESVKMVH 434
>CE28787 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 513
Score = 175 bits (444), Expect = 1e-43
Identities = 132/407 (32%), Positives = 197/407 (47%), Gaps = 71/407 (17%)
Query: 62 HEINEVNNNIRPQLLSLVQSDFFKYFKLDLYKECPFWSDNN--GYCVNRACAVDVVEDWD 119
HEI+E +NN R Q W++ G V A AV ED D
Sbjct: 124 HEISENHNNNRQQR----------------------WNETTKIGVIVQEAAAVKEEEDDD 161
Query: 120 SLPEYWQPEILGNIENATTDITDDECSFLDELCDKPRFEAEKDIEYCDTNDFNSQHSVLV 179
+ + NI+ + DDE LD + + + ++C+ D S V
Sbjct: 162 AEK---CADAGNNIDPMDRTLHDDEKRQLDAM---DHHDDGLEDKFCEIEDDESDGMHYV 215
Query: 180 DLTANPERFTGYGGEQSSQIWSSIYKENCFSLG---DEN---------QCLAKDAFYRLI 227
DL+ NPER+TGY G+ ++W SIY+ENCF D+N CL K FYRLI
Sbjct: 216 DLSKNPERYTGYAGKSPQRVWKSIYEENCFKPDPKFDKNFLTNPSNFGMCLEKRVFYRLI 275
Query: 228 SGLHASIGTHL----------SNEHLNTETGKWEPNLELFMTRVGNF-----PDRVSNIY 272
SGLH++I + S ++ G W N E+F R G P R+ N+Y
Sbjct: 276 SGLHSAITISIAAYNYKPPPPSLGQFGSQMGTWFRNTEMFAGRFGTKWSWEGPQRLRNVY 335
Query: 273 FNFAVVAKALWKIRPYM-NELGFCNAYNEDV--KGMIDGVVSQLNS--KVFNEDLLFH-- 325
F + + +AL K PY+ NEL + ED + ++ ++ ++ + F+E +F
Sbjct: 336 FIYLLELRALLKAAPYLQNELFYTGNDVEDAETRKAVEDLLEEIRAYPNHFDESEMFTGV 395
Query: 326 DEVSGQLKDDFRIRFKNVTKIMDCVQCDRCRLWGKVQTTGYATSLKILFEMDNDDDVARQ 385
+ + L+++FR F N+++IMDCV+CD+CRLWGKVQT G T+LKILF +
Sbjct: 396 ESHARALREEFRSHFVNISRIMDCVECDKCRLWGKVQTHGMGTALKILFSDLPHSHYKQD 455
Query: 386 HVVDKLTKYELIALFNTFDRLSKSVEAVNRF-EEMYNYQLKSPGEKL 431
+LT+ E++AL +F R S S+ V+ F E+MY PGE +
Sbjct: 456 SSKFQLTRNEVVALLQSFGRYSSSILEVDNFREDMY------PGESV 496
>ECU04g0170 [OU] KOG2608 Endoplasmic reticulum membrane-associated
oxidoreductin involved in disulfide bond formation
Length = 325
Score = 55.8 bits (133), Expect = 2e-07
Identities = 61/241 (25%), Positives = 103/241 (42%), Gaps = 44/241 (18%)
Query: 171 FNSQHSVLVDLTANPERFTGYGGEQ-SSQIWSSIYKENCFSLGDENQCLAKDAFYRLISG 229
FN ++ ++L E +TGY S++IW I++ + E+ L L+SG
Sbjct: 74 FNDEY---INLLETREAYTGYKTNTGSAEIWRKIWE-----ISREDTLLPT-----LVSG 120
Query: 230 LHASIGTHLSNEHLNTETGKWEPNLELFMTRVGNFPDRVSNIYFNFAVVAKALWKIRPYM 289
L SI THLS+ H N G + PN LF R + + N Y + +V + I
Sbjct: 121 LQFSILTHLSSFHRNF-FGTYLPNPVLFRKRFRD--NHRLNFYLTYFLVRSCVGNIDVDQ 177
Query: 290 NELGFCNAYNEDVKGMIDGVVSQLNSKVFNEDLLFHDEVSGQLKDDFRIRFKNVTKIMDC 349
+EL G + ++ + ++ ++ ++ ++ R + +++
Sbjct: 178 SELS----------GGLSSIIHAIRAQGSTNWVIQPVDLEKSIQ-----RVDEMIRLLKY 222
Query: 350 VQCDRCRLWGKVQTTGYATSLKILFEMDNDDDVARQHVVDKLTKYELIALFNTFDRLSKS 409
V C++C+LWG +Q G +LK+ F D L E L N F RLS S
Sbjct: 223 VNCEKCQLWGTIQLKGLRAALKV-FSGSTD-----------LESLERFFLVNFFMRLSVS 270
Query: 410 V 410
V
Sbjct: 271 V 271
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,522,993
Number of Sequences: 60738
Number of extensions: 1599369
Number of successful extensions: 4843
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4792
Number of HSP's gapped (non-prelim): 13
length of query: 561
length of database: 30,389,216
effective HSP length: 112
effective length of query: 449
effective length of database: 23,586,560
effective search space: 10590365440
effective search space used: 10590365440
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)