ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2549 good E KOG0573 Amino acid transport and metabolism Asparagine synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2549 869754 871298 515
(515 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML096w [E] KOG0573 Asparagine synthase 558 e-159
SPBC4F6.11c [E] KOG0573 Asparagine synthase 206 9e-53
ECU04g0840 [E] KOG0573 Asparagine synthase 130 5e-30
Hs9506697 [E] KOG0573 Asparagine synthase 124 3e-28
7289856 [E] KOG0573 Asparagine synthase 118 2e-26
Hs19718772 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 99 2e-20
CE06195 [E] KOG0573 Asparagine synthase 97 4e-20
CE09642 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 87 6e-17
At3g47340 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 81 3e-15
At5g65010 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 78 3e-14
CE04760 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 78 4e-14
At5g10240 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 76 1e-13
SPBC119.10 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing) 74 7e-13
>YML096w [E] KOG0573 Asparagine synthase
Length = 525
Score = 558 bits (1438), Expect = e-159
Identities = 299/523 (57%), Positives = 377/523 (71%), Gaps = 12/523 (2%)
Query: 1 MCGILAYFGKSFGSTLTDEFQELYEKDLTGPIQSCNSNTLFNELIPYIVRRGPNYASLRS 60
MCGIL ++ + + L DE E E G N +++FN++IPYI RGPNY+SLR+
Sbjct: 1 MCGILLHYCPN-NNYLNDELIEFPEGTEFGDTTCTNESSIFNKIIPYIAARGPNYSSLRA 59
Query: 61 LSEINAMLFSSVLSLRTPFTKQSVMVQDRYLLQYNGELYNDDTVENCEHSDTEYISSLL- 119
+ FSSVLSLR PFTKQS+ V DRY LQ+NGELYN + + +D+ YI+S+L
Sbjct: 60 VKAYRISWFSSVLSLRQPFTKQSINVDDRYFLQFNGELYNKEISQG--DNDSLYIASMLQ 117
Query: 120 ---EKHSVEEVVRKCYGEFAYAITDLQERKVYFGRDSIGKRSLSY-ILLDDELYITSVSG 175
E V +V++ GE+AY I D+ K+YFGRD IG+RSLSY + D+ELY+ SV+G
Sbjct: 118 NLKEGMGVIDVIKSLEGEYAYTIYDVNSSKLYFGRDPIGRRSLSYSVTPDNELYVASVTG 177
Query: 176 RCDGFQNCLAGVIYIFDINTKQLTGSVKIHDEPYIVSDQIDNDFQLLEEYKIKLYECLRR 235
FQ+C+ GVIY +D TK L + + H PY V+ +ID +F L E LY LR
Sbjct: 178 SAGSFQDCIGGVIYEYDTRTKLLNSNQRSH-LPYEVTSEIDLNFTSLSEVSKNLYAVLRD 236
Query: 236 SVYQRIVTIHPMHLENGNISILFSGGLDCSVITALLCEQLIKIN-RNSETIIELLNVGFE 294
SV +R+ +IHP H+EN I++LFSGG+DCSVI AL+CE L + + + + +IELLNV FE
Sbjct: 237 SVKKRVESIHPRHIENSPIAVLFSGGIDCSVIVALICEVLQENDYKCGKPVIELLNVSFE 296
Query: 295 NPRTGKMPSDSPDRILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKDTVIDLMYPKN 354
NPRTG PSD+PDR L+I SA+ L LYP V+IKL+EVDVPY +YL K VI+LMYPK
Sbjct: 297 NPRTGLFPSDTPDRKLSINSAKTLQNLYPNVDIKLVEVDVPYDEYLKWKPFVINLMYPKQ 356
Query: 355 TEMDLSIAIAFYFASRGRGKVTE-NGESKPYNRSGIVMFSGLGADELYGGYHKFSNKSNE 413
TEMDLSIAIAF+FASRGRG +T NGE PY R GIV+FSGLGADELYGGYHKF+NK
Sbjct: 357 TEMDLSIAIAFFFASRGRGFLTSLNGERTPYQRHGIVLFSGLGADELYGGYHKFANKPPH 416
Query: 414 ELVIELQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFS-VDLPLNYKVNKHIL 472
ELV EL +QI I+DRNLNRDDKVI+++G+EVRYPFLDE V++ S ++P+N+KVNK IL
Sbjct: 417 ELVEELTRQINNIYDRNLNRDDKVIAHNGVEVRYPFLDEYVIKLSTAEIPINFKVNKLIL 476
Query: 473 RVMAKDILRLDFISEEPKRAIQFGARSAKMTKDSNKRGTDLLK 515
R +A L+LD IS EPKRAIQFGA+SAKMTKD NK GTDLLK
Sbjct: 477 RKVASQYLKLDGISSEPKRAIQFGAKSAKMTKDGNKHGTDLLK 519
>SPBC4F6.11c [E] KOG0573 Asparagine synthase
Length = 548
Score = 206 bits (523), Expect = 9e-53
Identities = 170/530 (32%), Positives = 259/530 (48%), Gaps = 75/530 (14%)
Query: 41 FNELIPYIVRRGPNYASLRSLSE--INAMLFSSVLSLRTP---FTKQSVMVQDRYLLQYN 95
F+ L I RGP++ L+ N SSVL LR P T Q + +L +N
Sbjct: 21 FSALRKRIRARGPDFFGKHKLNSGPWNLHFESSVLHLRGPSDHLTPQPHVDSFGNVLCWN 80
Query: 96 GELYNDDTVE------NCEHSDTEYISSLLEKH--SVEEVVRKCYGEFAYAITDLQERKV 147
GE++ + + N +D + LL + +E+++ G FA+ ++ +
Sbjct: 81 GEIWQINHSDHHKFTLNRNENDGAKLFELLNNNPGDIEKILGSIQGPFAFVYYQVRTNTL 140
Query: 148 YFGRDSIGKRSLSYILLDDELYITSVSGRCD------GFQNCLAGVI--YIFDINTKQL- 198
++GRD +G+RSL Y L D ++SV D GF + +I F + QL
Sbjct: 141 WWGRDRLGRRSLLYSLRDSNFVLSSVGNSSDFREVEPGFHSVQVDLIPKINFSKSLMQLE 200
Query: 199 -TGSVKIHDEPYIVSDQIDNDFQLLEEYKIKLYECLRRSVYQRIVTIHPMHLEN------ 251
T + DE +++ + L Y IK ++ R++TI + N
Sbjct: 201 KTNPLPPVDESFLMGSISSDTVDTLHTYLIK-------ALQDRVLTIPKLCSSNLDCSHY 253
Query: 252 GNISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNVGFENPR---------TGKMP 302
+ +L+SGG+DC V+ L+ + I N+E+I +L+NV FENPR TG +P
Sbjct: 254 SRVCVLYSGGVDCGVLARLMHD----IVPNNESI-DLINVAFENPRFTETYRDRETGLLP 308
Query: 303 SD------SPDRILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKDTVIDLMYPKNTE 356
+ PDR ++ Q L + P + ++VPYT+ ++ V L+YP ++
Sbjct: 309 DNFDAYDVCPDRQTGLQGWQELISVCPARKWNFVAINVPYTEVCEAQEIVKTLIYPNDSV 368
Query: 357 MDLSIAIAFYFASRGRGKVTENGES-------KPYNRSGIVMFSGLGADELYGGYHK--- 406
MDLSI +AFYFAS+GRG + +N + Y V+ SGLGADE GGY +
Sbjct: 369 MDLSIGLAFYFASQGRGVLLQNVDDIKLKHQLPSYTIKAKVLISGLGADEQLGGYMRHLR 428
Query: 407 -FSNKSNEELVIELQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFSVDLPLNY 465
F K L ELQ I +I RNL RDD+V+SN G EVRYPFLDER++ +
Sbjct: 429 AFERKGMAGLEEELQLDIDRIPHRNLGRDDRVMSNQGKEVRYPFLDERLMALFSKMKTTD 488
Query: 466 KV-------NKHILRVMAKDILRLDFISEEPKRAIQFGARSAKMTKDSNK 508
K+ +K ILR + + L S+E KRAIQFG+++AKMT + +
Sbjct: 489 KMRLDLVGGDKLILRELGRR-LGCPLASQEKKRAIQFGSKAAKMTPGTGR 537
>ECU04g0840 [E] KOG0573 Asparagine synthase
Length = 474
Score = 130 bits (327), Expect = 5e-30
Identities = 137/513 (26%), Positives = 228/513 (43%), Gaps = 111/513 (21%)
Query: 43 ELIPYIVRRGPNYASLRSLSEINAMLFSSVLSLRTPFTKQSVMVQDRYLLQYNGELYNDD 102
E+ I RG +++S + A+ SSVLS+R + ++ YL YNGE+YN
Sbjct: 16 EMALLIGNRGGDHSSKVCTDGVVAI--SSVLSIRGSVGQP--VLGAGYLFLYNGEIYNG- 70
Query: 103 TVENCEHSDTEYISSLLEKHSVEEVVRKCYG-------------------EFAYAITDLQ 143
E SDT +I KH+V+ ++++C G E A+ +
Sbjct: 71 -----EPSDTLFI-----KHTVDRLLQECSGLGESEGDIVDRIYEEINRHENEMAVVLML 120
Query: 144 ERKVYFGRDSIGKRSLSYILLDDELYITSVSGRCDGFQNCLAGVIYIFDINTKQLTGSVK 203
+VYF +D IG+RSL Y L D Y++SV G + L IY ++ T++L K
Sbjct: 121 GDRVYFFKDDIGRRSLGYGL--DPFYVSSV-GYTEEVNPML---IYSYNKATRRLDEWPK 174
Query: 204 --------IHDEPYIVSDQIDNDFQLLEEYKIKLYEC----------------------- 232
I I+S D + +Y + C
Sbjct: 175 NSRIVKRYISLGGAILSRLCDAKYSKKYQYLRRHLVCGKVEGSRTELNDVNAVISGFSRL 234
Query: 233 LRRSVYQRIVTIHPMHLENGNISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNVG 292
RRSV +RI+ GN+ + FSGG+D ++ L + + I L+N
Sbjct: 235 FRRSVERRIL--------EGNVCVFFSGGVDSMLVAVFL-----HYVASPQQKIYLINTS 281
Query: 293 FENPRTGKMPSDSPDRILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKDTVIDLMYP 352
F S DR + + L + + + + DV + + K+ + L+YP
Sbjct: 282 F---------GPSWDRDAGRRGFEELCSRFGKRSFVFVPNDVTIEEVRAAKEHIYRLIYP 332
Query: 353 KNTEMDLSIAIAFYFASRGRGKVTENGESKPYNRSGIVMFSGLGADELYGGYHKFSNKSN 412
K+ MD +I +F +R ES+ Y R V + G GADE++GGYHK+ +
Sbjct: 333 KSGPMDFNIGATLFFTAR---------ESRKYGR---VAYLGSGADEMFGGYHKYKDSCF 380
Query: 413 EELVIELQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFSVDLPLNYKVNKHIL 472
E ++ + I NL RDD+VIS+ +E R+PFLD +V++S+++ + +
Sbjct: 381 RE---DMMFDLFTISHHNLCRDDRVISDSQVECRFPFLDSELVEYSLEIGSSVIMMNGER 437
Query: 473 RVMAKDILR---LDFISEEPKRAIQFGARSAKM 502
+ + +++LR L S PK+A+Q+G+ +K+
Sbjct: 438 KFVIREVLRRNGLGSASTIPKKAMQYGSGMSKI 470
>Hs9506697 [E] KOG0573 Asparagine synthase
Length = 643
Score = 124 bits (311), Expect = 3e-28
Identities = 100/305 (32%), Positives = 148/305 (47%), Gaps = 55/305 (18%)
Query: 253 NISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNVGF----------------ENP 296
N++ILFSGG+D S++ A L ++ I ++ I+LLNV F +
Sbjct: 321 NVAILFSGGID-SMVIATLADRHIPLDEP----IDLLNVAFIAEEKTMPTTFNREGNKQK 375
Query: 297 RTGKMPSDS-------------------PDRILAIKSAQLLSELYPQVNIKLIEVDVPYT 337
++PS+ PDRI + L + P +E++V
Sbjct: 376 NKCEIPSEEFSKDVAAAAADSPNKHVSVPDRITGRAGLKELQAVSPSRIWNFVEINVSME 435
Query: 338 DYLSHKDT-VIDLMYPKNTEMDLSIAIAFYFASRGRGKVTENGESKPYNRSGIVMFSGLG 396
+ + T + L+ P +T +D SI A +FASRG G + K Y + V+ +G+G
Sbjct: 436 ELQKLRRTRICHLIRPLDTVLDDSIGCAVWFASRGIGWLVAQEGVKSYQSNAKVVLTGIG 495
Query: 397 ADELYGGYHK----FSNKSNEELVIELQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDE 452
ADE GY + F + E L E+ ++ +I RNL RDD+VI +HG E R+PFLDE
Sbjct: 496 ADEQLAGYSRHRVRFQSHGLEGLNKEIMMELGRISSRNLGRDDRVIGDHGKEARFPFLDE 555
Query: 453 RVVQFSVDLPLNYKVN---------KHILRVMAKDILRLDFISEEPKRAIQFGARSAKMT 503
VV F LP+ K N K +LR+ A + L L + PKRA+QFG+R AKM
Sbjct: 556 NVVSFLNSLPIWEKANLTLPRGIGEKLLLRLAAVE-LGLTASALLPKRAMQFGSRIAKME 614
Query: 504 KDSNK 508
K + K
Sbjct: 615 KINEK 619
Score = 53.1 bits (126), Expect = 1e-06
Identities = 44/154 (28%), Positives = 75/154 (48%), Gaps = 9/154 (5%)
Query: 37 SNTLFNELIPYIVRRGPNYASLRSLSEINAM-LFSS-VLSLRTPFTKQSVMVQDRYLLQY 94
S L +L+ + +RGPN + S++N LFS+ VL LR T Q V + + +
Sbjct: 16 SQDLKEDLLYNLKQRGPNSSKQLLKSDVNYQCLFSAHVLHLRGVLTTQPVEDERGNVFLW 75
Query: 95 NGELYNDDTVENCEHSDTE----YISSLLEKHSVEEVVRKCYGEFAYAITDLQERKVYFG 150
NGE+++ VE E +DT+ Y+SS + + + + G +++ ++FG
Sbjct: 76 NGEIFSGIKVE-AEENDTQILFNYLSSCKNESEILSLFSEVQGPWSFIYYQASSHYLWFG 134
Query: 151 RDSIGKRSL--SYILLDDELYITSVSGRCDGFQN 182
RD G+RSL + L ++SV + G N
Sbjct: 135 RDFFGRRSLLWHFSNLGKSFCLSSVGTQTSGLAN 168
>7289856 [E] KOG0573 Asparagine synthase
Length = 564
Score = 118 bits (295), Expect = 2e-26
Identities = 92/286 (32%), Positives = 145/286 (50%), Gaps = 41/286 (14%)
Query: 251 NGNISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNVGFENPRTGKMPS---DSPD 307
+ + ILFSGGLDC+++ ALL Q + +N IEL+NV FE+ + + D PD
Sbjct: 297 HAKLCILFSGGLDCTIL-ALLANQFVPVNEP----IELINVAFESVKGQNISEKLFDVPD 351
Query: 308 RILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKDTVID-LMYPKNTEMDLSIAIAFY 366
R ++ S L +L P+ L++V+V + H + I L+YP +T +D S+ AF+
Sbjct: 352 RKTSLVSVNELKQLCPERCWNLLKVNVTRLELQQHLTSRIKHLIYPLDTVLDESLGCAFW 411
Query: 367 FASRGRGKVTENGESKPYNRSGIVMFSGLGADELYGGYHKFSNKSNEELVIELQKQIA-- 424
FAS V G GADEL+GGY ++ N + L +L++Q+A
Sbjct: 412 FASHCTHSTAR------------VALIGSGADELFGGYTRYRNSYSRCLGNDLERQLAVQ 459
Query: 425 --------QIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFSVDLPLNYKV--------- 467
+I RNL RDD++I++ G R PF++E V+F L + K
Sbjct: 460 NELERDWQRISARNLARDDRIIADTGKTARSPFIEENFVKFIRSLEVYQKCCFGFPEGVG 519
Query: 468 NKHILRVMAKDILRLDFISEEPKRAIQFGARSAKMTKDSNKRGTDL 513
+K +LR+ I D + + KRAIQFG+R A ++ + + +L
Sbjct: 520 DKLLLRLYGYQIGLRDVVFLK-KRAIQFGSRIANKKQNGSHQSDNL 564
>Hs19718772 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 561
Score = 99.0 bits (245), Expect = 2e-20
Identities = 115/447 (25%), Positives = 188/447 (41%), Gaps = 77/447 (17%)
Query: 92 LQYNGELYNDDTVENCEHSDTEY--------ISSLLEKHSVEEVVRKCYGEFAYAITDLQ 143
L YNGE+YN ++ +H + EY I L +K +E+ + G FA+ + D
Sbjct: 72 LCYNGEIYNHKKMQ--QHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTA 129
Query: 144 ERKVYFGRDSIGKRSLSYILLDDELYITSVSGRCDGF-------------QNCLAGVIYI 190
+KV+ GRD+ G R L + +D +V G + L G +
Sbjct: 130 NKKVFLGRDTYGVRPLFKAMTEDG--FLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEV 187
Query: 191 FDINTKQLTGSVKI------HDEP-YIVSDQIDNDFQ--LLEEYKIKLYECLRRSVYQRI 241
D+ SV++ DEP + + D ++ F +E K L +V +R+
Sbjct: 188 LDLKPNGKVASVEMVKYHHCRDEPLHALYDNVEKLFPGFEIETVKNNLRILFNNAVKKRL 247
Query: 242 VTIHPMHLENGNISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNVGFENPRTGKM 301
+T + I L SGGLD S++ A L +QL + + ++ +G E
Sbjct: 248 MT-------DRRIGCLLSGGLDSSLVAATLLKQLKE--AQVQYPLQTFAIGME------- 291
Query: 302 PSDSPDRILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKDTVIDLMYPKNTEMDLSI 361
DSPD + A K A + + +V E + + +T Y T +
Sbjct: 292 --DSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLET-----YDITT---VRA 341
Query: 362 AIAFYFASRGRGKVTENGESKPYNRSGIVMFSGLGADELYGGYHKFSN-KSNEELVIELQ 420
++ Y S+ K N +V+FSG G+DEL GY F S E+ E +
Sbjct: 342 SVGMYLISKYIRK----------NTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESE 391
Query: 421 KQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFSVDLPLNYK-----VNKHILRVM 475
+ + +++ ++ R D+ + HG+E+R PFLD R + + LP + + KH+LR
Sbjct: 392 RLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRET 451
Query: 476 AKDI-LRLDFISEEPKRAIQFGARSAK 501
+D L I PK A G S K
Sbjct: 452 FEDSNLIPKEILWRPKEAFSDGITSVK 478
>CE06195 [E] KOG0573 Asparagine synthase
Length = 475
Score = 97.4 bits (241), Expect = 4e-20
Identities = 81/295 (27%), Positives = 149/295 (50%), Gaps = 26/295 (8%)
Query: 235 RSVYQRIVTIHPMHLEN---GNISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNV 291
+ ++Q+++ + L+N ++++ SGG+D + + ++ + N + I+L+NV
Sbjct: 179 KDLFQKVIDATKILLKNYHESHVAVCLSGGVDSTFVAHVVHSS---VPENMQ--IDLINV 233
Query: 292 GFENPRTGKMPSDSPDRILAIKSAQLLSELYPQVNIKLIEVDVP-YTDYLSHKDTVIDLM 350
F N K +PDR A K+ + YP +LI V+V T ++ K+++ D
Sbjct: 234 AFGNSE--KECEQAPDRKRARKALESFRTAYPTRQFRLILVNVDSQTLEVNRKESISDAA 291
Query: 351 YPKNTEMDLSIAIAFYFASRGRGKVTENGESKPYNRSGIVMFSGLGADELYGGYHKFSNK 410
P ++ +D S++ +FA R G +EN +S G GADEL GY + +
Sbjct: 292 QPASSVLDDSLSCVLWFAVRAEGVDSENQQS--VRSPATTCLLGSGADELLAGYARHRTR 349
Query: 411 SNEELVIE-----LQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFSVDLPLNY 465
+E + E + ++ ++ RN RD +V + G + P L++ VV + LP++
Sbjct: 350 FEKEQIPENVAEECENELRRLGSRNGGRDARVAAQLGKTILSPLLEDTVVTWLNALPVDS 409
Query: 466 KVNKHILR-VMAKDILR-----LDFISEEPKRAIQFGARSAKMTK--DSNKRGTD 512
K + + R V K +LR L + PK+A+QFG+R AKM+ D++ +G+D
Sbjct: 410 KWDLSLPRGVGEKQLLRETVKMLGSPYDAPKQAMQFGSRMAKMSNAGDNSIKGSD 464
>CE09642 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 551
Score = 87.0 bits (214), Expect = 6e-17
Identities = 129/500 (25%), Positives = 206/500 (40%), Gaps = 105/500 (21%)
Query: 1 MCGILAYFGKSFGSTLTDEFQELYEKDLTGPIQSCNSNTLFNELIPYIVRRGPNYASLRS 60
MCGI A G+ +T + Q+ + K IV RGP+ L
Sbjct: 1 MCGIWAIIGEQ----VTPQQQDAFMK---------------------IVGRGPDLTVLEE 35
Query: 61 LSEINAMLFSSVLSLRTPFTKQSV--MVQDRYLLQYNGELYNDDTVE-NCEH------SD 111
+ N L L++ P S +V + NGE+YN +++ +C SD
Sbjct: 36 VQP-NVHLGFHRLAIVMPGDTPSAQPIVGGGLSVVCNGEIYNHQSLKIDCPFTLKNGGSD 94
Query: 112 TEYISSLLEKHS--VEEVVRKCYGEFAYAITDLQERKVYFGRDSIGKRSLSY-------I 162
I + KH+ ++E G FA+ + D ++ VY GRD IG R L Y +
Sbjct: 95 CAAIITSFIKHNKDLKEACASLDGVFAFIMAD--DKNVYIGRDPIGVRPLFYGYNSNGSL 152
Query: 163 LLDDELY-ITSVSGRCDGFQNCLAGVIYIFDINTKQLTGSVKIHDEPYIVSDQIDNDFQL 221
L+ E+ I + R + F I +F + + + Y I + F
Sbjct: 153 LIGSEVKCIEELCERVEYFPPGCCATISLF-------APTRPVQPQQYYSVPTIADRFLS 205
Query: 222 LEEYKIKLYECLRRSVYQRIVTIHPMHLENGNISILFSGGLDCSVITALLCEQLIKINRN 281
++ K + + L +SV +R+ + N N + SGGLD S+I ++ L
Sbjct: 206 IDCTKTLVRDVLVKSVEKRL-------MGNRNFGFMLSGGLDSSLIASIATRFL-----K 253
Query: 282 SETIIELLNVGFENPRTGKMPSDSPDRILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLS 341
+ I +VGFE DSPD A K A L I +V
Sbjct: 254 QKPIA--FSVGFE---------DSPDLENAKKVADYLK----------IPHEVLVITPQQ 292
Query: 342 HKDTVIDLMYPKNTEMDLSI--AIAFYFASRGRGKVTENGESKPYNRSGIVMFSGLGADE 399
D + ++++ T L I IA Y + ++++ + K V+ SG GADE
Sbjct: 293 CIDIIPEVVFALETFDPLIIRCGIAHYLLCQ---HISKSSDVK-------VLLSGEGADE 342
Query: 400 LYGGYHKFSNKSNE-ELVIELQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFS 458
L+G Y N L E+ +++ +H ++ R D+ S HG+E+R PFLD+R +
Sbjct: 343 LFGSYAYMQRAPNALHLHKEILRRMHHLHQYDVLRCDRSTSCHGLEIRVPFLDKRFIDLV 402
Query: 459 VDLPLNY-----KVNKHILR 473
LP +Y K+ KH+LR
Sbjct: 403 SRLPPSYKLMPMKLEKHVLR 422
>At3g47340 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 584
Score = 81.3 bits (199), Expect = 3e-15
Identities = 122/508 (24%), Positives = 206/508 (40%), Gaps = 91/508 (17%)
Query: 1 MCGILAYFGKSFGSTLTDEFQELYEKDLTGPIQSCNSNTLFNELIPYIVRRGPNYASLRS 60
MCGILA G S D+ Q + L EL + RGP+++ L
Sbjct: 1 MCGILAVLGCS------DDSQAKRVRVL--------------ELSRRLRHRGPDWSGLYQ 40
Query: 61 LSEINAMLFSSVLSLRTPFTKQSVMVQDRYLL-QYNGELYNDDTVENC-------EHSDT 112
+ N + + + Q + +D+ ++ NGE+YN + + SD
Sbjct: 41 NGD-NYLAHQRLAVIDPASGDQPLFNEDKTIVVTVNGEIYNHEELRKRLKNHKFRTGSDC 99
Query: 113 EYISSLLEKHSVEEVVRKCYGEFAYAITDLQERKVYFGRDSIGKRSLSYIL--LDDELYI 170
E I+ L E++ V+ V G F++ + D ++ RD+IG SL YI LD ++I
Sbjct: 100 EVIAHLYEEYGVD-FVDMLDGIFSFVLLDTRDNSFMVARDAIGVTSL-YIGWGLDGSVWI 157
Query: 171 TS----VSGRCDGFQNCLAGVIYIFDINTKQLTGSVKIHDEPYIVSDQIDNDFQLLEEYK 226
+S ++ C+ F+ G Y + +L G + ++ P+ ++ L
Sbjct: 158 SSEMKGLNDDCEHFETFPPGHFY-----SSKLGGFKQWYNPPWFNESVPSTPYEPLA--- 209
Query: 227 IKLYECLRRSVYQRIVTIHPMHLENGNISILFSGGLDCSVITALLCEQLI--KINRNSET 284
+ +V +R++T P +L SGGLD S++ ++ L K +
Sbjct: 210 --IRRAFENAVIKRLMTDVPF-------GVLLSGGLDSSLVASITARHLAGTKAAKQWGP 260
Query: 285 IIELLNVGFENPRTGKMPSDSPDRILAIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKD 344
+ VG E SPD + A+ L ++ + + + + D + H +
Sbjct: 261 QLHSFCVGLEG---------SPDLKAGKEVAEYLGTVHHEFHFSVQDGIDAIEDVIYHVE 311
Query: 345 TVIDLMYPKNTEMDLSIAIAFYFASRGRGKVTENGESKPYNRSGIVM-FSGLGADELYGG 403
T +T M L S+ G+ M SG GADE++GG
Sbjct: 312 TYDVTTIRASTPMFLM--------------------SRKIKSLGVKMVLSGEGADEIFGG 351
Query: 404 Y---HKFSNKSNEELVIELQKQIAQIHDRNLNRDDKVISNHGIEVRYPFLDERVVQFSVD 460
Y HK NK +E E ++I +H + R +K S G+E R PFLD+ + ++
Sbjct: 352 YLYFHKAPNK--KEFHQETCRKIKALHKYDCLRANKSTSAFGLEARVPFLDKDFINTAMS 409
Query: 461 LPLNYKVNKHILRVMAKDILRLDFISEE 488
L K+ K + K +LR F EE
Sbjct: 410 LDPESKMIKPEEGRIEKWVLRRAFDDEE 437
>At5g65010 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 578
Score = 78.2 bits (191), Expect = 3e-14
Identities = 115/467 (24%), Positives = 195/467 (41%), Gaps = 73/467 (15%)
Query: 43 ELIPYIVRRGPNYASLRSLSEINAMLFSSVLSLRTPFT-KQSVMVQDRYL-LQYNGELYN 100
EL + RGP+++ L + L L++ P + Q + +D+ + + NGE+YN
Sbjct: 23 ELSRRLRHRGPDWSGLHCYED--CYLAHERLAIIDPTSGDQPLYNEDKTVAVTVNGEIYN 80
Query: 101 DDTVENC-------EHSDTEYISSLLEKHSVEEVVRKCYGEFAYAITDLQERKVYFGRDS 153
+ SD E I+ L E+H EE + G FA+ + D +++ RD+
Sbjct: 81 HKILREKLKSHQFRTGSDCEVIAHLYEEHG-EEFIDMLDGMFAFVLLDTRDKSFIAARDA 139
Query: 154 IGKRSLSYIL--LDDELYITS----VSGRCDGFQNCLAGVIYIFDINTKQLTGSVKIHDE 207
IG L YI LD ++ S +S C+ F + G IY ++KQ G ++
Sbjct: 140 IGITPL-YIGWGLDGSVWFASEMKALSDDCEQFMSFPPGHIY----SSKQ--GGLRRWYN 192
Query: 208 PYIVSDQIDNDFQLLEEYKIKLYECLRRSVYQRIVTIHPMHLENGNISILFSGGLDCSVI 267
P ++Q+ + + L ++V +R++T P +L SGGLD S++
Sbjct: 193 PPWYNEQVPST----PYDPLVLRNAFEKAVIKRLMTDVPF-------GVLLSGGLDSSLV 241
Query: 268 TALLCEQLIKIN--RNSETIIELLNVGFENPRTGKMPSDSPDRILAIKSAQLLSELYPQV 325
A+ L K R + + +G + SPD + A L + +
Sbjct: 242 AAVALRHLEKSEAARQWGSQLHTFCIGLQG---------SPDLKAGREVADYLGTRHHEF 292
Query: 326 NIKLIEVDVPYTDYLSHKDTVIDLMYPKNTEMDLSIAIAFYFASRGRGKVTENGESKPYN 385
+ + + + H +T +T M L S+
Sbjct: 293 QFTVQDGIDAIEEVIYHIETYDVTTIRASTPMFLM--------------------SRKIK 332
Query: 386 RSGIVM-FSGLGADELYGGY---HKFSNKSNEELVIELQKQIAQIHDRNLNRDDKVISNH 441
G+ M SG G+DE+ GGY HK NK +E E ++I +H + R +K S
Sbjct: 333 SLGVKMVLSGEGSDEILGGYLYFHKAPNK--KEFHEETCRKIKALHQFDCLRANKSTSAW 390
Query: 442 GIEVRYPFLDERVVQFSVDLPLNYKVNKHILRVMAKDILRLDFISEE 488
G+E R PFLD+ + ++ + +K+ K L + K +LR F EE
Sbjct: 391 GVEARVPFLDKEFLNVAMSIDPEWKLIKPDLGRIEKWVLRNAFDDEE 437
>CE04760 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 567
Score = 77.8 bits (190), Expect = 4e-14
Identities = 92/386 (23%), Positives = 164/386 (41%), Gaps = 61/386 (15%)
Query: 94 YNGELYNDDTVENCE--------HSDTEYISSLLEKHSVEEVVRKCYGEFAYAITDLQER 145
+NGE+YN + + E H D+E I L EK+ + G FA+ + +
Sbjct: 77 HNGEIYNHQELRDTELKKYNLKTHCDSEVIIFLYEKYRDGHICNLLDGVFAFVLCC--DG 134
Query: 146 KVYFGRDSIGKRSLSYILLDDELYITSVSGRCDGFQNCLAGVIYIFDINTKQL--TGSVK 203
RD +G + + Y + DD Y S +C +C I F TG V+
Sbjct: 135 DFLAARDPLGVKQMYYGIDDDGRYFFSNEMKCLE-DSCGDNKIESFPPGHYYTPKTGFVR 193
Query: 204 IHDEPYIVSDQIDNDFQ-LLEEYKIKLYECLRRSVYQRIVTIHPMHLENGNISILFSGGL 262
+ + DF+ + +KL L++++ I ++H + + I +L SGGL
Sbjct: 194 YFNPEWF-------DFRKAIHPLDLKL---LQKTM---IASVHKRLMSDAPIGVLLSGGL 240
Query: 263 DCSVITALLCEQLIKINRNSETIIELLNVGFENPRTGKMPSDSPDRILAIKSAQLLSELY 322
D S+++++ ++ + + ++G ++ +SPD + A K A+ + +
Sbjct: 241 DSSLVSSIASREM----KRRGMAVHSFSIGVDH--------NSPDVVAARKVAKFIGTTH 288
Query: 323 PQVNIKLIEVDVPYTDYLSHKDTVIDLMYPKNTEMDLSIAIAFYFASRGRGKVTENGESK 382
+ + E + H ++ +T M YF S+
Sbjct: 289 HEFYFSIEEGIKNLRKLIWHLESYDVTSIRASTPM--------YFL------------SE 328
Query: 383 PYNRSGI-VMFSGLGADELYGGYHKFSN-KSNEELVIELQKQIAQIHDRNLNRDDKVISN 440
+ GI V+ SG GADE++GGY F N S+E+ E ++ ++ + R DK
Sbjct: 329 EIRKLGIKVVLSGEGADEIFGGYLYFHNAPSDEDFQKETIDRVLHLYTSDCLRADKSSMA 388
Query: 441 HGIEVRYPFLDERVVQFSVDLPLNYK 466
H +EVR PFLD+ V+ +V L +K
Sbjct: 389 HSVEVRVPFLDKAFVEAAVSLDPAFK 414
>At5g10240 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 578
Score = 75.9 bits (185), Expect = 1e-13
Identities = 113/467 (24%), Positives = 199/467 (42%), Gaps = 73/467 (15%)
Query: 43 ELIPYIVRRGPNYASLRSLSEINAMLFSSVLSLRTPFT-KQSVMVQDRYL-LQYNGELYN 100
EL + RGP+++ L + L L++ P + Q + +D+ + + NGE+YN
Sbjct: 23 ELSRRLRHRGPDWSGLHCYED--CYLAHERLAIVDPTSGDQPLYNEDKTIAVTVNGEIYN 80
Query: 101 DDTV-ENCEH------SDTEYISSLLEKHSVEEVVRKCYGEFAYAITDLQERKVYFGRDS 153
+ EN + SD E I+ L E+H EE V G FA+ + D +++ RD+
Sbjct: 81 HKALRENLKSHQFRTGSDCEVIAHLYEEHG-EEFVDMLDGMFAFVLLDTRDKSFIAARDA 139
Query: 154 IGKRSLSYIL--LDDELYITS----VSGRCDGFQNCLAGVIYIFDINTKQLTGSVKIHDE 207
IG L YI LD ++ S +S C+ F G IY ++KQ G ++
Sbjct: 140 IGITPL-YIGWGLDGSVWFASEMKALSDDCEQFMCFPPGHIY----SSKQ--GGLRRWYN 192
Query: 208 PYIVSDQIDNDFQLLEEYKIKLYECLRRSVYQRIVTIHPMHLENGNISILFSGGLDCSVI 267
P S+ + + + + ++V +R++T P +L SGGLD S++
Sbjct: 193 PPWFSEVVPST----PYDPLVVRNTFEKAVIKRLMTDVPF-------GVLLSGGLDSSLV 241
Query: 268 TALLCEQLIKINRNSE--TIIELLNVGFENPRTGKMPSDSPDRILAIKSAQLLSELYPQV 325
++ L K + + + +G + SPD + A L + ++
Sbjct: 242 ASVALRHLEKSEAACQWGSKLHTFCIGLKG---------SPDLKAGREVADYLGTRHHEL 292
Query: 326 NIKLIEVDVPYTDYLSHKDTVIDLMYPKNTEMDLSIAIAFYFASRGRGKVTENGESKPYN 385
+ + + + + H +T +T M L S+
Sbjct: 293 HFTVQDGIDAIEEVIYHVETYDVTTIRASTPMFLM--------------------SRKIK 332
Query: 386 RSGIVM-FSGLGADELYGGY---HKFSNKSNEELVIELQKQIAQIHDRNLNRDDKVISNH 441
G+ M SG G+DE++GGY HK NK +E E ++I +H + R +K S
Sbjct: 333 SLGVKMVLSGEGSDEIFGGYLYFHKAPNK--KEFHEETCRKIKALHQYDCLRANKSTSAW 390
Query: 442 GIEVRYPFLDERVVQFSVDLPLNYKVNKHILRVMAKDILRLDFISEE 488
G+E R PFLD+ + ++ + +K+ + L + K +LR F E+
Sbjct: 391 GVEARVPFLDKEFINVAMSIDPEWKMIRPDLGRIEKWVLRNAFDDEK 437
>SPBC119.10 [E] KOG0571 Asparagine synthase (glutamine-hydrolyzing)
Length = 557
Score = 73.6 bits (179), Expect = 7e-13
Identities = 104/410 (25%), Positives = 164/410 (39%), Gaps = 62/410 (15%)
Query: 89 RYLLQYNGELYNDDTV-ENCE-------HSDTEYISSLLEKHSVEEVVRKCYGEFAYAIT 140
+ +L NGE+YN + EN + +SD E I L +H G F++ +
Sbjct: 69 KLVLTVNGEIYNHLKLRENLKGNYKFKTYSDCEVILYLYREHG-PACANMLDGMFSWVLY 127
Query: 141 DLQERKVYFGRDSIGKRSLSYILLDDELYITSVSGRCDGFQNCLAGVI-----YIFDINT 195
D + KV RD IG +L D + +I + +D T
Sbjct: 128 DQDKDKVVAARDPIGITTLYQGFSSDSPDTAYFASELKALHPVCDKIIAFPPGHYYDSET 187
Query: 196 KQLTGSVKIH--DEPYIVSDQIDNDFQLLEEYKIKLYECLRRSVYQRIVTIHPMHLENGN 253
KQ K DE I S+ +D YK+ L E L SV +R++ P
Sbjct: 188 KQTVRYFKPSWWDENKIPSNPVD--------YKL-LRETLEASVRKRLMAEVPY------ 232
Query: 254 ISILFSGGLDCSVITALLCEQLIKINRNSETIIELLNVGFENPRTGKMP---SDSPDRIL 310
+L SGGLD S+I ++ + K+ NS + E P+ SPD +
Sbjct: 233 -GVLLSGGLDSSLIASIAARETEKL-ANSTSQSEEARTITAWPKLHSFAIGLPGSPDLLA 290
Query: 311 AIKSAQLLSELYPQVNIKLIEVDVPYTDYLSHKDTVIDLMYPKNTEMDLSIAIAFYFASR 370
A K A L + + + E D + H +T +T M Y SR
Sbjct: 291 ARKVADFLHTFHHEHTFTIDEGLDALRDVIYHLETYDVTTIRASTPM--------YLLSR 342
Query: 371 GRGKVTENGESKPYNRSGIVMFSGLGADELYGGYHKFSN-KSNEELVIELQKQIAQIHDR 429
K+ G ++ SG G+DE++GGY F N S E E +++ +H
Sbjct: 343 ---KIKAQGVK--------MVLSGEGSDEIFGGYLYFGNAPSREAFHSECVRRVKNLHLS 391
Query: 430 NLNRDDKVISNHGIEVRYPFLDERVVQFSVDLP------LNYKVNKHILR 473
+ R +K G+E R PFLD+ ++ ++++ +N + K+ILR
Sbjct: 392 DCLRANKSTMAWGLEARVPFLDKDFLEVALNIDPEEKMYINGRKEKYILR 441
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,461,988
Number of Sequences: 60738
Number of extensions: 1322018
Number of successful extensions: 3463
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 3413
Number of HSP's gapped (non-prelim): 21
length of query: 515
length of database: 30,389,216
effective HSP length: 111
effective length of query: 404
effective length of database: 23,647,298
effective search space: 9553508392
effective search space used: 9553508392
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)