ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2742 good I KOG4569 Lipid transport and metabolism Predicted lipase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2742 932344 933471 376
(376 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR107w [I] KOG4569 Predicted lipase 362 e-100
At5g18630 [I] KOG4569 Predicted lipase 64 5e-10
At5g18640 [I] KOG4569 Predicted lipase 63 8e-10
At4g18550 [I] KOG4569 Predicted lipase 59 2e-08
At2g42450 [IOT] KOG2088 Predicted lipase/calmodulin-binding heat... 55 2e-07
CE22233 [I] KOG4569 Predicted lipase 53 8e-07
CE02240 [I] KOG4569 Predicted lipase 52 2e-06
CE01305 [IOT] KOG2088 Predicted lipase/calmodulin-binding heat-s... 52 2e-06
CE18012 [I] KOG4569 Predicted lipase 51 2e-06
At1g06800 [I] KOG4569 Predicted lipase 50 7e-06
>YJR107w [I] KOG4569 Predicted lipase
Length = 328
Score = 362 bits (929), Expect = e-100
Identities = 174/317 (54%), Positives = 227/317 (70%), Gaps = 7/317 (2%)
Query: 60 YEKMVYFSKICALTYCISTGRLEMDKTFFDGGCPADLDFCSNEELNPSIRRTRVELILEA 119
YE++VYF K +++ CIS L ++KTF DGGCP ++FC++E +NP+ +T VEL+L A
Sbjct: 17 YERLVYFIKASSISSCISDNLLLVNKTFNDGGCPPHINFCNDEIINPTAPQTVVELVLNA 76
Query: 120 DEQELGTGYVAVDHEREVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEI 179
+ ELG+GY+AVDH ++VV+LAFRGS+TRQDWFSDFEIYP Y P+ KEY+KL+E G+I
Sbjct: 77 KKGELGSGYLAVDHGKKVVILAFRGSTTRQDWFSDFEIYPVNYSPLCVKEYRKLIEEGKI 136
Query: 180 SACHNCMIHKGFYRFIETLSKDFLQRVERIFKRYPDYNLVVTGHSLGAALASICGIELKL 239
C C +H+GF RF ETL D +++E I + +P+Y +VVTGHSLGAALAS+ GIELK+
Sbjct: 137 RECEGCKMHRGFLRFTETLGMDVFKKMESILESFPEYRIVVTGHSLGAALASLAGIELKI 196
Query: 240 RGYNPLILTYATPKIFNEEMKQWVNDLFDTKAIHDECVETGEVNMLHGYFRVIHLQDYIP 299
RG++PL+LT+ATPKIFN EMKQWV++LF+T AI E + E+ GYFRV+H DYIP
Sbjct: 197 RGFDPLVLTFATPKIFNSEMKQWVDELFETDAIEKESILKDEIQFRKGYFRVVHTGDYIP 256
Query: 300 MVPPGYKAAGLEIFITKPELPHEIHDLEYRAVGSGATWKKVPMNKDSKYALMSG-IGHWL 358
MVPP Y AGLE+FI K LP D+EYR + T KD MSG + WL
Sbjct: 257 MVPPFYHPAGLEMFINKVGLPQNAEDIEYRGKNNRLTL------KDGFREGMSGLVEDWL 310
Query: 359 HMDEHRKYFILINSCSG 375
H+ EHR YFI + CSG
Sbjct: 311 HVYEHRAYFIDVVGCSG 327
>At5g18630 [I] KOG4569 Predicted lipase
Length = 348
Score = 63.5 bits (153), Expect = 5e-10
Identities = 50/185 (27%), Positives = 83/185 (44%), Gaps = 40/185 (21%)
Query: 128 YVAVDHEREVVMLAFRGSSTR--QDWFSDFEIYPTQYKPISTKEYKKLVERGEISACHNC 185
YV V + +++AFRG+ Q+W SD +K + Y + +
Sbjct: 81 YVGVAKDLNAIIIAFRGTQEHSIQNWVSDLF-----WKQLDLN-YPDMPD---------A 125
Query: 186 MIHKGFYRFIE--TLSKDFLQRVERIFKRY-PDYNLVVTGHSLGAALASICGIELKLR-- 240
M+H GFY TL L + R+ K Y + N++VTGHS+G A+AS CG++L +
Sbjct: 126 MVHHGFYSAYHNTTLRPAVLDAITRVKKVYGANINIIVTGHSMGGAMASFCGLDLVVNEG 185
Query: 241 GYNPLILTYATPKIFNEEMKQWVNDLFDTKAIHDECVETGEVNMLHGYFRVIHLQDYIPM 300
N ++T+ P++ N + + L + FR+ H +D +P
Sbjct: 186 EENVQVMTFGQPRVGNAAFASYYSLL------------------VPNTFRITHDRDMVPH 227
Query: 301 VPPGY 305
+PP Y
Sbjct: 228 LPPYY 232
>At5g18640 [I] KOG4569 Predicted lipase
Length = 369
Score = 62.8 bits (151), Expect = 8e-10
Identities = 53/200 (26%), Positives = 90/200 (44%), Gaps = 42/200 (21%)
Query: 113 VELILEADEQELGTGYVAVDHEREVVMLAFRGSSTR--QDWFSDFEIYPTQYKPISTKEY 170
+E+I+ D + YV V + +++AFRG+ Q+W SD +K + Y
Sbjct: 89 IEIIV--DVEHCLQAYVGVAKDLNAIIIAFRGTQEHSIQNWVSDLF-----WKQLDLN-Y 140
Query: 171 KKLVERGEISACHNCMIHKGFYRFIE--TLSKDFLQRVERIFKRY-PDYNLVVTGHSLGA 227
+ + M+H GFY T+ L V+R + Y + N++VTGHS+G
Sbjct: 141 PDMPD---------AMVHHGFYSAYHNTTVRPAVLDAVKRAKESYGANLNIMVTGHSMGG 191
Query: 228 ALASICGIELKLR--GYNPLILTYATPKIFNEEMKQWVNDLFDTKAIHDECVETGEVNML 285
A+AS C ++L + N ++T+ P++ N + N L +
Sbjct: 192 AMASFCALDLVVNEGEENVQVMTFGQPRVGNAAFASYFNLL------------------V 233
Query: 286 HGYFRVIHLQDYIPMVPPGY 305
FR+IH +D +P +PP Y
Sbjct: 234 PNTFRIIHDRDIVPHLPPYY 253
>At4g18550 [I] KOG4569 Predicted lipase
Length = 419
Score = 58.5 bits (140), Expect = 2e-08
Identities = 58/227 (25%), Positives = 96/227 (41%), Gaps = 61/227 (26%)
Query: 127 GYVAVDHE-------REVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEI 179
GYVAV + R +++++RGS +W DFE K ER +
Sbjct: 131 GYVAVTDDQGTALLGRRDIVVSWRGSVQPLEWVEDFEFG-------LVNAIKIFGERND- 182
Query: 180 SACHNCMIHKGFYR----------FIETLSKD-FLQRVERIFKRYPDYNLVVT--GHSLG 226
IH+G+Y F +T ++D L+ V R+ ++Y D + +T GHSLG
Sbjct: 183 ----QVQIHQGWYSIYMSQDERSPFTKTNARDQVLREVGRLLEKYKDEEVSITICGHSLG 238
Query: 227 AALASICGIELKLRGYN-----------PLILTYATPKIFNEEMKQWVNDLFDTKAIHDE 275
AALA++ ++ GYN +A+P++ + + ++ + L D + +
Sbjct: 239 AALATLSATDIVANGYNRPKSRPDKSCPVTAFVFASPRVGDSDFRKLFSGLEDIRVL--- 295
Query: 276 CVETGEVNMLHGYFRVIHLQDYIPMVPP-GYKAAGLEIFITKPELPH 321
R +L D IP+ PP GY G E I + P+
Sbjct: 296 --------------RTRNLPDVIPIYPPIGYSEVGDEFPIDTRKSPY 328
>At2g42450 [IOT] KOG2088 Predicted lipase/calmodulin-binding heat-shock
protein
Length = 508
Score = 55.1 bits (131), Expect = 2e-07
Identities = 42/158 (26%), Positives = 73/158 (45%), Gaps = 25/158 (15%)
Query: 128 YVAVDHEREVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEISACHNCMI 187
Y+ VDH R++V+ RG+ T D +D I + + ++ + Y G A +
Sbjct: 194 YIGVDHRRKLVVFGIRGTHTIYDLITD--IVSSSDEEVTFEGYS--THFGTAEAARWFLN 249
Query: 188 HKGFYRFIETLSKDFLQRVERIFKRYPDYNLVVTGHSLGAALASICGIELKLR-----GY 242
H+ LQ + R +Y Y L + GHSLG A+AS+ I LK G+
Sbjct: 250 HE-------------LQTIRRCLAKYEGYKLRLVGHSLGGAIASLMAIMLKKMPREELGF 296
Query: 243 NPLILT---YATPKIFNEEMKQWVNDLFDTKAIHDECV 277
+ I++ YATP ++E+ + ++ T + D+ +
Sbjct: 297 DAEIISAVGYATPPCVSKELAENCSEFVTTIVMQDDII 334
>CE22233 [I] KOG4569 Predicted lipase
Length = 238
Score = 52.8 bits (125), Expect = 8e-07
Identities = 48/180 (26%), Positives = 74/180 (40%), Gaps = 30/180 (16%)
Query: 127 GYVAVDHEREVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEISACHNCM 186
G+ + D ++VV+++FRG+ + + T KP A H
Sbjct: 22 GFTSFDTTQKVVVMSFRGTQGATQLTEEILDFFTGKKPFFN------------DAGH--- 66
Query: 187 IHKGFY-RFIETLSKDFLQRVERIFKRYPDYNLVVTGHSLGAALASICGIELKLRGYNPL 245
I FY F + Q + R+ +YP+Y L VTGHSLG A+ASI + G
Sbjct: 67 IFTYFYDAFFFLWNGGLQQEIRRLKYQYPEYELWVTGHSLGGAIASIAASYVVHSG---- 122
Query: 246 ILTYATPKIFNEEMKQWVNDLFDTKAIHDECVETGEVNMLHGYFRVIHLQDYIPMVPPGY 305
Y ++ M Q +D HD+ FR++H +D +P +PP Y
Sbjct: 123 --LYTGDQVKLVTMGQPRTGDYDYAVWHDKTFPYS--------FRIVHHRDIVPHIPPQY 172
>CE02240 [I] KOG4569 Predicted lipase
Length = 336
Score = 51.6 bits (122), Expect = 2e-06
Identities = 40/180 (22%), Positives = 78/180 (43%), Gaps = 34/180 (18%)
Query: 128 YVAVDHEREVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEISACHNCMI 187
++A+ V+++FRG+++ S+F + Y + EI +N +
Sbjct: 88 FIAISDSTNQVIISFRGTNSGGQLLSEFGVGLEDYAAYT-----------EIDGSNNTVS 136
Query: 188 --HKGFYRFIETLSKDFLQRVERIFKRYPDYNLVVTGHSLGAALASICGIELKLRGYNPL 245
H Y F++ +++ + V+ K +Y ++TGHSLG A+A++ + R ++
Sbjct: 137 VGHVNVY-FLDAMNQMWEDMVQPSIKNRQNYTFLITGHSLGGAMATLTAFRIAFRQFSSR 195
Query: 246 IL--TYATPKIFNEEMKQWVNDLFDTKAIHDECVETGEVNMLHGYFRVIHLQDYIPMVPP 303
I T+ P++ + + D M+ FRV+H D IP +PP
Sbjct: 196 IKVHTFGEPRVGDTVFASYFTD------------------MVPYAFRVVHNTDPIPHLPP 237
>CE01305 [IOT] KOG2088 Predicted lipase/calmodulin-binding heat-shock
protein
Length = 681
Score = 51.6 bits (122), Expect = 2e-06
Identities = 38/144 (26%), Positives = 70/144 (48%), Gaps = 12/144 (8%)
Query: 129 VAVDHEREVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEISACHNCMIH 188
V DH+++ +++ RGS + D +D + + + + L E EI + +H
Sbjct: 360 VIADHDKKSIVITIRGSCSLIDLVTDLSL-EDELMTVDVDQDATLREDEEIDKRGDVRVH 418
Query: 189 KGFYR----FIETLSKDFLQRVERIFKRYPDYNLVVTGHSLGAALASICGIELKLRGYNP 244
+G R +TL+K+ + + +F P Y LVV GHSLGA + S+ + LK +
Sbjct: 419 RGMLRSARYVFDTLNKNKI--LNDLFISNPSYQLVVCGHSLGAGVGSLLTMLLKQEYPSV 476
Query: 245 LILTYATP-----KIFNEEMKQWV 263
+ +A P + +EM+++V
Sbjct: 477 ICYAFAPPGCVISEFGQDEMEKYV 500
>CE18012 [I] KOG4569 Predicted lipase
Length = 265
Score = 51.2 bits (121), Expect = 2e-06
Identities = 50/182 (27%), Positives = 74/182 (40%), Gaps = 39/182 (21%)
Query: 130 AVDHEREVVMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYKKLVERGEISACHNCMIHK 189
AVD ++V++++FR ++T +F Y K Y I +
Sbjct: 40 AVDTTQKVLVMSFRATNTGTQLEEEFLNYFVAKKAFFDSGY----------------IFE 83
Query: 190 GFYRFIETLSKDFLQRVERIFK-RYPDYNLVVTGHSLGAALASI-CGIELKLRGYNP--- 244
FY L K L+ R K RYPDY + VTGHSLGAALAS+ +K + P
Sbjct: 84 FFYDAYLALWKGGLEAEMRNLKYRYPDYEVWVTGHSLGAALASVGASWVVKTGLFKPEQM 143
Query: 245 LILTYATPKIFNEEMKQWVNDLFDTKAIHDECVETGEVNMLHGYFRVIHLQDYIPMVPPG 304
+LT P+ + W + F FR++H D +P +P
Sbjct: 144 KLLTAGQPRTGDYAYSNWHQNTFAYS------------------FRIVHAHDMVPHLPFQ 185
Query: 305 YK 306
Y+
Sbjct: 186 YE 187
>At1g06800 [I] KOG4569 Predicted lipase
Length = 515
Score = 49.7 bits (117), Expect = 7e-06
Identities = 44/168 (26%), Positives = 73/168 (43%), Gaps = 33/168 (19%)
Query: 127 GYVAVDHEREV---------VMLAFRGSSTRQDWFSDFEIYPTQYKPISTKEYK-----K 172
GYVAV + E + +A+RG+ TR +W +D + + KP+S ++
Sbjct: 192 GYVAVSDDNEATRCRLGRRDIAIAWRGTVTRLEWIADLKDF---LKPVSGNGFRCPDPAV 248
Query: 173 LVERGEISACHNCMIHKGFYRFIETLSKDFLQRVERIFKRYPD-----YNLVVTGHSLGA 227
E G + + F +F + + L V+R+ +RY D ++ VTGHSLG
Sbjct: 249 KAESGFLDLYTDKDTSCNFSKF--SAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGG 306
Query: 228 ALASICGIELKLRGYN---------PLILTYATPKIFNEEMKQWVNDL 266
ALA + ++ G N TY P++ N K+ + L
Sbjct: 307 ALAVLSAYDVAEMGVNRTRKGKVIPVTAFTYGGPRVGNIRFKERIEKL 354
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.139 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,312,655
Number of Sequences: 60738
Number of extensions: 1082003
Number of successful extensions: 2356
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2343
Number of HSP's gapped (non-prelim): 10
length of query: 376
length of database: 30,389,216
effective HSP length: 108
effective length of query: 268
effective length of database: 23,829,512
effective search space: 6386309216
effective search space used: 6386309216
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)