ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2868 good O KOG3151 Posttranslational modification, protein turnover, chaperones 26S proteasome regulatory complex, subunit RPN12/PSMD8
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2868 976003 975209 -265
(265 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFR052w [O] KOG3151 26S proteasome regulatory complex subunit RP... 230 1e-60
Hs4506233 [O] KOG3151 26S proteasome regulatory complex subunit ... 103 3e-22
CE06608 [O] KOG3151 26S proteasome regulatory complex subunit RP... 89 6e-18
7294091 [O] KOG3151 26S proteasome regulatory complex subunit RP... 89 8e-18
At1g64520 [O] KOG3151 26S proteasome regulatory complex subunit ... 86 5e-17
7294333 [O] KOG3151 26S proteasome regulatory complex subunit RP... 65 1e-10
At5g42040 [O] KOG3151 26S proteasome regulatory complex subunit ... 60 3e-09
>YFR052w [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 274
Score = 230 bits (587), Expect = 1e-60
Identities = 125/276 (45%), Positives = 169/276 (60%), Gaps = 13/276 (4%)
Query: 1 MPSLPELTKGLKTAFEGKQYETCXXXXXXXXXXXXXXXXXXPDIKSAKENQSYLQDLNIA 60
MPSL ELTK L AFE Y C PD+ +N YL DL I
Sbjct: 1 MPSLAELTKSLSIAFENGDYAACEKLLPPIKIELIKNNLLIPDLSI--QNDIYLNDLMIT 58
Query: 61 KKILEIGALSSIYCEDFETFMNYYSQLSAFYFSDVKELSDSDSKFKXXXXXXXXXXAQND 120
K+ILE+GAL+SI +F++F NY++QL +YFS+ +LS+SD K K +QN+
Sbjct: 59 KRILEVGALASIQTFNFDSFENYFNQLKPYYFSNNHKLSESDKKSKLISLYLLNLLSQNN 118
Query: 121 YTKLHSEFEFLSRYVTNLEESSYLSYPVKLERLLMEGSYQKAWDLLQTGSK-VEEFNVFT 179
TK HSE ++L +++ NLE+ S LSYP+KL+R LMEGSYQKAWDLLQ+GS+ + EF+ FT
Sbjct: 119 TTKFHSELQYLDKHIKNLEDDSLLSYPIKLDRWLMEGSYQKAWDLLQSGSQNISEFDSFT 178
Query: 180 KTLKEAIREEIALNSEQAYSSLPLFNAKTLLFFENEKEMEAFAKERDWSVNHGVITFGND 239
LK AIR+EIA N+E +Y LPL N K LLFF NEKE E FA ER+W + + + F N
Sbjct: 179 DILKSAIRDEIAKNTELSYDFLPLSNIKALLFFNNEKETEKFALERNWPIVNSKVYFNNQ 238
Query: 240 STP----------DRIESGDTVDMAIDYAVHLESIV 265
S + + + ++ A+DYA+ +E+IV
Sbjct: 239 SKEKADYEDEMMHEEDQKTNIIEKAMDYAISIENIV 274
>Hs4506233 [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 257
Score = 103 bits (257), Expect = 3e-22
Identities = 71/215 (33%), Positives = 107/215 (49%), Gaps = 7/215 (3%)
Query: 55 QDLNIAKKILEIGALSSIYCEDFETFMNYYSQLSAFYFSDVKELSDSDSKFKXXXXXXXX 114
Q L +A+ ILEIGA SI +D +F Y +QL +YF ++L +S +
Sbjct: 46 QQLILARDILEIGAQWSILRKDIPSFERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLF 105
Query: 115 XXAQNDYTKLHSEFEFLSRYVTNLEESSYLSYPVKLERLLMEGSYQKAWDLLQTGSKVEE 174
+QN + H+E E L +++ + Y+ +PV LE+ LMEGSY K + L + E
Sbjct: 106 LLSQNRVAEFHTELERLP--AKDIQTNVYIKHPVSLEQYLMEGSYNKVF-LAKGNIPAES 162
Query: 175 FNVFTKTLKEAIREEIALNSEQAYSSLPLFNAKTLLFFENEKEMEAFAKERDWSV--NHG 232
+ F L + IR+EIA E+AY + A +LFF K+M +AK+R W + N+
Sbjct: 163 YTFFIDILLDTIRDEIAGCIEKAYEKILFTEATRILFFNTPKKMTDYAKKRGWVLGPNNY 222
Query: 233 VITFGNDSTPD--RIESGDTVDMAIDYAVHLESIV 265
P+ I S + I+YA LE IV
Sbjct: 223 YSFASQQQKPEDTTIPSTELAKQVIEYARQLEMIV 257
>CE06608 [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 250
Score = 89.0 bits (219), Expect = 6e-18
Identities = 65/227 (28%), Positives = 113/227 (49%), Gaps = 13/227 (5%)
Query: 44 IKSAKENQSYLQDLNIAKKILEIGALSSIYCEDFETFMNYYSQLSAFYFSDVKELSDSDS 103
+ SA + + Q +K + EI L +I DFETF +Y +Q+ +Y +S++
Sbjct: 32 VLSASSDLNDKQSALASKDLYEISVLLAILKHDFETFDDYINQMHTYY---TMAPENSEN 88
Query: 104 KFKXXXXXXXXXXAQNDYTKLHSEFEFLSRYVTNLEESS--YLSYPVKLERLLMEGSYQK 161
K A N S+F L + E++S Y+S PV++E+ LMEG+Y K
Sbjct: 89 KHLMTGLHLMFLLAANRL----SDFHMLLEQIPQKEQTSNAYISTPVRIEQSLMEGAYNK 144
Query: 162 AWDLLQTGSKVEEFNVFTKTLKEAIREEIALNSEQAYSSLPLFNAKTLLFFENEKEMEAF 221
L + + +F + + + IR EIA + E+++ L +A +L F+N+++M+ F
Sbjct: 145 VV-LTEKNIPSPFYTIFIRIMLDTIRREIATSIEKSFKVLTAKDATVMLLFDNDEQMKKF 203
Query: 222 AKERDWSVNHGVITFGNDSTPDRIESGDTVDMA---IDYAVHLESIV 265
+ER W ++ F + ++ + DTV +A + YA LE IV
Sbjct: 204 GQERKWHLDGERYVFEIEVAQEKPVNLDTVRVATQTLFYAKQLEQIV 250
>7294091 [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 264
Score = 88.6 bits (218), Expect = 8e-18
Identities = 60/218 (27%), Positives = 105/218 (47%), Gaps = 6/218 (2%)
Query: 51 QSYLQDLNIAKKILEIGALSSIYCEDFETFMNYYSQLSAFYFSDVKELSDSDSKFKXXXX 110
QS + L +A+ +LE+ S+ +D F Y +QL +Y+ K + +S+SK+K
Sbjct: 50 QSSKKQLILARSVLEVAVEHSVLSKDLLAFERYMAQLKCYYYDYAKIIGESESKYKLLGL 109
Query: 111 XXXXXXAQNDYTKLHSEFEFLSRYVTNLEESSYLSYPVKLERLLMEGSYQKAWDLLQTGS 170
+ N + H+E E LS V ++ + ++ + LE+ +MEG Y K + T
Sbjct: 110 NLLYLLSGNRVSDFHTELELLS--VDVIQHNQFIRPILALEQYIMEGRYNKIFQAKST-V 166
Query: 171 KVEEFNVFTKTLKEAIREEIALNSEQAYSSLPLFNAKTLLFFENEKEMEAFAKERDWSVN 230
VE ++ F L E +R+EI E++Y + +A L E++AF +R W +
Sbjct: 167 PVEVYSYFMDLLLETVRDEIGACIEKSYDKISAKDAAKRLNLRVPDEIKAFGAKRQWKLE 226
Query: 231 -HGVITFGNDSTPDR--IESGDTVDMAIDYAVHLESIV 265
G +F + S + + S + + + YA LE IV
Sbjct: 227 ASGDYSFTDRSVKPKELLPSEELAEQVLSYARDLEMIV 264
>At1g64520 [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 267
Score = 85.9 bits (211), Expect = 5e-17
Identities = 69/272 (25%), Positives = 116/272 (42%), Gaps = 15/272 (5%)
Query: 2 PSLPELTKGL---KTAFEGKQYETCXXXXXXXXXXXXXXXXXXPDIKSAKENQSYLQDLN 58
P L E+++ K AF K Y TC P +++ + ++L
Sbjct: 3 PQLTEVSQQFERFKAAFARKDYNTCSDLLSQLKVLLTKFTSLPPLFENSP---NAAKELT 59
Query: 59 IAKKILEIGALSSIYCEDFETFMNYYSQLSAFYFSDVKELSDSDSKFKXXXXXXXXXXAQ 118
IA+ I E + S+ ED + F + QL +Y + S + Q
Sbjct: 60 IARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYVDARNRIPQSPQENLILGLNLLRLLVQ 119
Query: 119 NDYTKLHSEFEFLSRYVTNLEESSYLSYPVKLERLLMEGSYQKAWDLLQTGSKVEEFNVF 178
N + H+E E LS E + + V+LE+ MEG+Y + QT + F
Sbjct: 120 NRIAEFHTELELLSSATL---EDPCIKHAVELEQSFMEGAYNRVLSARQTAPDAT-YVYF 175
Query: 179 TKTLKEAIREEIALNSEQAYSSLPLFNAKTLLFFENEKEMEAFAKER--DWSVNHGVITF 236
L + IR+EIA SE+AY + + +A+ +L F +++E+ + + +W V G + F
Sbjct: 176 MDLLAKTIRDEIAGCSEKAYDYVSISDARQMLLFSSDQELLTYVTDEHPEWEVKEGFVVF 235
Query: 237 --GNDSTP-DRIESGDTVDMAIDYAVHLESIV 265
++ P I S ++ + YA LE IV
Sbjct: 236 QKAKETAPCKEIPSLQLINQTLSYARELERIV 267
>7294333 [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 262
Score = 65.1 bits (157), Expect = 1e-10
Identities = 59/212 (27%), Positives = 92/212 (42%), Gaps = 11/212 (5%)
Query: 60 AKKILEIGALSSIYCEDFETFMNYYSQLSAFYFSDVKELSDSDSKFKXXXXXXXXXXAQN 119
++++LEI SI +D+ F Y +QL+ +Y+ K L S +K A N
Sbjct: 56 SREMLEIAVEHSITIKDYAAFERYMAQLNTYYYDYDKYLESSKHMYKFMGLNLLYMLATN 115
Query: 120 DYTKLHSEFEFLSRYVTNLEESSYLSYPVKLERLLMEGSYQKAWDLLQTGSKV--EEFNV 177
H E E L + L S++ + LE MEG Y K +LQ + E ++
Sbjct: 116 RLADFHIELERLPTAL--LLHDSFIQPVLALENYYMEGRYNK---ILQAKKSMPSEIYSN 170
Query: 178 FTKTLKEAIREEIALNSEQAYSSL-PLFNAKTLLFFENEKEMEAFAKERDWSVN-HGVIT 235
F L REEIA E++Y + P A+ L KE+ A +R W ++ G
Sbjct: 171 FMDILVNTAREEIASCMEKSYLKMAPKLAAQRLGLRPGSKELFELATKRQWCLDEEGNYD 230
Query: 236 FGNDST--PDRIESGDTVDMAIDYAVHLESIV 265
+ T +++ + D + YA LE IV
Sbjct: 231 YAGLHTRPMEKVPAKDIATHNLTYAQELEKIV 262
>At5g42040 [O] KOG3151 26S proteasome regulatory complex subunit RPN12/PSMD8
Length = 159
Score = 60.1 bits (144), Expect = 3e-09
Identities = 43/153 (28%), Positives = 73/153 (47%), Gaps = 9/153 (5%)
Query: 118 QNDYTKLHSEFEFLSRYVTNLEESSYLSYPVKLERLLMEGSYQKAWDLLQTGSKVEEFNV 177
QN + H+E LS E+ + + V+LE+ MEG+Y + QT E +
Sbjct: 11 QNRIAEFHTELGLLSSATL---ENPCIKHAVELEQSFMEGAYNRVLSARQTAPD-ETYVY 66
Query: 178 FTKTLKEAIREEIALNSEQAYSSLPLFNAKTLLFFENEKEMEAFAKER--DWSVNHGVIT 235
F L + IR+EIA SE+AY L + +L F +++++ + E +W V G++
Sbjct: 67 FMDLLAKTIRDEIAGCSEKAYDHLSISEGCKMLLFSSDQQLLTYVNEEHPEWEVKDGLVV 126
Query: 236 F--GNDSTP-DRIESGDTVDMAIDYAVHLESIV 265
F ++ P I S ++ + Y LE I+
Sbjct: 127 FQKTRETAPCKEIPSLQLINQTLSYTRELERIL 159
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,618,809
Number of Sequences: 60738
Number of extensions: 503161
Number of successful extensions: 932
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 922
Number of HSP's gapped (non-prelim): 7
length of query: 265
length of database: 30,389,216
effective HSP length: 104
effective length of query: 161
effective length of database: 24,072,464
effective search space: 3875666704
effective search space used: 3875666704
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)