ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2877 good U KOG2635 Intracellular trafficking, secretion, and vesicular transport Medium subunit of clathrin adaptor complex
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2877 978018 976411 -536
(536 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFR051c [U] KOG2635 Medium subunit of clathrin adaptor complex 436 e-122
Hs11863154 [U] KOG2635 Medium subunit of clathrin adaptor complex 202 8e-52
7290211 [U] KOG2635 Medium subunit of clathrin adaptor complex 194 4e-49
CE01778 [U] KOG2635 Medium subunit of clathrin adaptor complex 191 2e-48
SPCC285.08 [U] KOG2635 Medium subunit of clathrin adaptor complex 166 8e-41
At5g05010 [U] KOG2635 Medium subunit of clathrin adaptor complex 104 3e-22
YPL259c [U] KOG0937 Adaptor complexes medium subunit family 53 1e-06
>YFR051c [U] KOG2635 Medium subunit of clathrin adaptor complex
Length = 546
Score = 436 bits (1120), Expect = e-122
Identities = 252/549 (45%), Positives = 337/549 (60%), Gaps = 18/549 (3%)
Query: 1 MVVLAVSITTRKGKPLLSRQFRDITKDRVMELLSNFQTLVANSSKQHTFVEDEHVRYVYK 60
MVVLA SITTR+GKPLLSRQF+D++KDRV+ELLSNFQ LV+ S HTFVED+HVRYVY+
Sbjct: 1 MVVLAASITTRQGKPLLSRQFKDLSKDRVLELLSNFQNLVSEISSDHTFVEDKHVRYVYR 60
Query: 61 PFDDYYIILITNLHSNIIQDMDTLNIFVQTVDSTLKGLSEEDVFDSAFDILNSFDEIITM 120
PFD+YYIILITN SNII+D+ TLN+F QT++S L ++++F +AF+IL+SFDEI++M
Sbjct: 61 PFDNYYIILITNRQSNIIKDLATLNLFSQTINSYLSSFQDQEIFHNAFEILSSFDEIVSM 120
Query: 121 G-YKENLSLTQIKTFLAMESHEERIQEIIXXXXXXXXXXXXXXXXXXXXXXXQARKLGIP 179
G YKENLS TQ++T+L+MESHEERIQEII RK G
Sbjct: 121 GGYKENLSFTQVQTYLSMESHEERIQEIIERNKEIEATEERKRRAKEIARKEHERKHGFM 180
Query: 180 DTQ-EFTGIHGGVMNKA-----XXXXXXXXXXXXXXXXXXXXXNXXXXXXXXXXXXXXXX 233
+ ++ G + + +K +
Sbjct: 181 SSNGDYDGANRFMGSKDPNVTNAINSYYSHASPAAQQSYLQSSHAAAAEVAPVASPMATS 240
Query: 234 XYANREPLGGKGLQVNGPRRGVERPIKNVERQSLSVQTATLPEEEKPINNGILISVKETI 293
A GG L RR P + S S T PEE+ P NNGILIS+KE I
Sbjct: 241 QRAGHSATGGMKLGGGAGRRAGAAPRPSAI-SSASSGTPPPPEEDVPENNGILISIKEVI 299
Query: 294 NAEISRDGAIMGSELKGVLELRVNNPDLAHAKLCLGTDVNVKDKTYQFKTHPNVDKAGFL 353
NAE SRDG I SELKGVLELR+N+ DL+H+ L L ++V+DK++QFKTHPN+DK FL
Sbjct: 300 NAEFSRDGTIHSSELKGVLELRINDHDLSHSNLKLADSIDVRDKSFQFKTHPNIDKQSFL 359
Query: 354 TNKTIGLRDPNKPFPSNDQSLGVLRWRKVKGADEHDLIPLEISTWLSPSSETDNAIDVTF 413
+ K I LRD +K FP+NDQSLGVLRWRKV A++ LIPL ++TW+SP SE+ DV
Sbjct: 360 STKLISLRDKSKAFPANDQSLGVLRWRKVAPAEDDSLIPLTLTTWVSP-SESQQGFDVII 418
Query: 414 EYEVNSDYEGTIDELRFLIPVF-TDTVFIKDDLNE-ANAIVEAIDESQGIIIKVDPIESG 471
EYE S E + ++ F IPVF + V I + + ++A V +D+ G IK+ I +
Sbjct: 419 EYE--SVLETELADVIFTIPVFPQEPVDINTESSTCSDAEVVNMDQEMGTSIKISKIAAN 476
Query: 472 QSSVFSVTVQAAYEDALFPIEVALKNSN----AKPLSNLNIASVVSAAD-DTELPFDTVS 526
+ + T++A YEDAL+P+ V+ + S AK + + I SVV A D D ELP+D ++
Sbjct: 477 DAGALAFTIEAPYEDALYPMTVSFQESTRDKLAKSFTGMAIQSVVMANDHDQELPYDVIT 536
Query: 527 TLKSEQYVV 535
+LKS++Y+V
Sbjct: 537 SLKSDEYLV 545
>Hs11863154 [U] KOG2635 Medium subunit of clathrin adaptor complex
Length = 511
Score = 202 bits (515), Expect = 8e-52
Identities = 147/539 (27%), Positives = 253/539 (46%), Gaps = 31/539 (5%)
Query: 1 MVVLAVSITTRKGKPLLSRQFRDITKDRVMELLSNFQTLVANSSKQHTFVEDEHVRYVYK 60
MV+LA ++ T+ GK ++SRQF ++T+ R+ LL+ F L+ N+ KQHTFVE E VRYVY+
Sbjct: 1 MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLM-NTGKQHTFVETESVRYVYQ 59
Query: 61 PFDDYYIILITNLHSNIIQDMDTLNIFVQTVDSTLKGLSEEDVFDSAFDILNSFDEIITM 120
P + Y++LIT +SNI++D++TL +F + + + L E ++ + FD++ +FDEI+ +
Sbjct: 60 PMEKLYMVLITTKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVAL 119
Query: 121 GYKENLSLTQIKTFLAMESHEERIQEIIXXXXXXXXXXXXXXXXXXXXXXXQARKLGIPD 180
GY+EN++L QI+TF M+SHE +++ QAR+
Sbjct: 120 GYRENVNLAQIRTFTEMDSHE---EKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQ 176
Query: 181 TQEFTGIHGGVMNKAXXXXXXXXXXXXXXXXXXXXXNXXXXXXXXXXXXXXXXXYANREP 240
++ G GG + A
Sbjct: 177 GKKAPGF-GGFGSSAVSGGSTAAMITETIIETDKPKVAPAPARPSGPSKALK-------- 227
Query: 241 LGGKGLQVNGPRRGVERPIKNVERQSLSVQTATLPEEEKPINN--GILISVKETINAEIS 298
LG KG +V+ ++ + + S+ +T+ + P N + + ++E I
Sbjct: 228 LGAKGKEVDNFVDKLKSEGETIMSSSMGKRTSEATKMHAPPINMESVHMKIEEKITLTCG 287
Query: 299 RDGAIMGSELKGVLELRVNNPDLAHAKLCLGTDVNVKDKTYQFKTHPNVDKAGFLTNKTI 358
RDG + EL G++ LR+++ +L + N K Q +THPNVDK F I
Sbjct: 288 RDGGLQNMELHGMIMLRISDDKYGRIRLHVE---NEDKKGVQLQTHPNVDKKLFTAESLI 344
Query: 359 GLRDPNKPFPSNDQSLGVLRWRKVKGADEHDLIPLEISTWLSPSSETDNAIDVTFEYEVN 418
GL++P K FP N +GVL+WR E IPL I+ W SE+ N DV EYE+
Sbjct: 345 GLKNPEKSFPVN-SDVGVLKWR--LQTTEESFIPLTINCW---PSESGNGCDVNIEYELQ 398
Query: 419 SDYEGTIDELRFLIPVFTDT-VFIKDDLNEANAIVEAIDESQGIIIKVDPIESGQSSVFS 477
D ++++ IP+ + + +++ + + + +D S FS
Sbjct: 399 ED-NLELNDVVITIPLPSGVGAPVIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFS 457
Query: 478 VTVQAAYEDALFPIEVALKNSNAKPLSNLNIASVVSAADDTELPFDTVSTLKSEQYVVV 536
+ A + FP++V+ + K N+ + V ++ + F T +T ++Y ++
Sbjct: 458 I---AGQPNDFFPVQVSFVSK--KNYCNIQVTKVTQVDGNSPVRFSTETTFLVDKYEIL 511
>7290211 [U] KOG2635 Medium subunit of clathrin adaptor complex
Length = 532
Score = 194 bits (492), Expect = 4e-49
Identities = 148/551 (26%), Positives = 257/551 (45%), Gaps = 34/551 (6%)
Query: 1 MVVLAVSITTRKGKPLLSRQFRDITKDRVMELLSNFQTLVANSSKQHTFVEDEHVRYVYK 60
MV++A ++ T+ GK +LSRQF ++TK R+ LL+ F L+ + KQHT+VE + VRYVY+
Sbjct: 1 MVLIAAAVCTKNGKVILSRQFVEMTKARIEGLLAAFPKLMT-AGKQHTYVETDSVRYVYQ 59
Query: 61 PFDDYYIILITNLHSNIIQDMDTLNIFVQTVDSTLKGLSEEDVFDSAFDILNSFDEIITM 120
P + Y++LIT SNI++D++TL +F + + L E+++ ++AF+++ +FDEI+ +
Sbjct: 60 PMEKLYMLLITTKASNILEDLETLRLFSKVIPEYSHSLDEKEIVENAFNLIFAFDEIVAL 119
Query: 121 GYKENLSLTQIKTFLAMESHEERI-QEIIXXXXXXXXXXXXXXXXXXXXXXXQARKLGIP 179
GY+E+++L QIKTF+ M+SHEE++ Q + +A K G P
Sbjct: 120 GYRESVNLAQIKTFVEMDSHEEKVYQAVRQTQERDARQKMREKAKELQRQRMEASKRGGP 179
Query: 180 DTQEFTGIHGGVMNKAXXXXXXXXXXXXXXXXXXXXXNXXXXXXXXXXXXXXXXXYANRE 239
GG + N
Sbjct: 180 SLGGIGSRSGGF---SADGIGSSGVSSSSGASSANTGITSIDVDTKSKAAASKPASRNAL 236
Query: 240 PLGGKGLQVNG---PRRGVERPIKNVERQSLSVQTATLPEEEKPINNGILISV-KETINA 295
LGGK V+ + I N+ + + ++ I + KE+++
Sbjct: 237 KLGGKSKDVDSFVDQLKNEGEKIANLAPAAPAGGSSAAASASAAAKAAIASDIHKESVHL 296
Query: 296 EI--------SRDGAIMGSELKGVLELRVNNPDLAHAKLCLGTDVNVKDKTYQFKTHPNV 347
+I RDG + E G+L LR+ D A+ ++ L N + Q +THPNV
Sbjct: 297 KIEDKLVVRLGRDGGVQQFENSGLLTLRIT--DEAYGRILLKLSPN-HTQGLQLQTHPNV 353
Query: 348 DKAGFLTNKTIGLRDPNKPFPSNDQSLGVLRWRKVKGADEHDLIPLEISTWLSPSSETDN 407
DK F + TIGL++ KPFP N +GVL+WR V + + +PL I+ W PS +
Sbjct: 354 DKELFKSRTTIGLKNLGKPFPLN-TDVGVLKWRFV--SQDESAVPLTINCW--PSDNGEG 408
Query: 408 AIDVTFEYEVNSDYEGTIDELRFLIPVFTDTVFIKDDLNEANAIVEAIDESQGIIIKVDP 467
DV EYE+ + + + ++ +IP+ + ++ + E + D + ++ P
Sbjct: 409 GCDVNIEYELEAQ-QLELQDVAIVIPL---PMNVQPSVAEYDGTYN-YDSRKHVLQWHIP 463
Query: 468 I--ESGQSSVFSVTVQAAYEDALFPIEVALKNSNAKPLSNLNIASVVSAADDTELPFDTV 525
I + +S + A+ FP++V+ + P + + VV + + + +
Sbjct: 464 IIDAANKSGSMEFSCSASIPGDFFPLQVSFVSKT--PYAGVVAQDVVQVDSEAAVKYSSE 521
Query: 526 STLKSEQYVVV 536
S L E+Y +V
Sbjct: 522 SILFVEKYEIV 532
>CE01778 [U] KOG2635 Medium subunit of clathrin adaptor complex
Length = 515
Score = 191 bits (486), Expect = 2e-48
Identities = 139/562 (24%), Positives = 258/562 (45%), Gaps = 73/562 (12%)
Query: 1 MVVLAVSITTRKGKPLLSRQF-RDITKDRVMELLSNFQTLVAN----SSKQHTFVEDEHV 55
MV++A I ++ GK L++RQF D+ + R+ L+ F L+ N +++QHTFVE + V
Sbjct: 1 MVLIAACILSKTGKLLVARQFVNDMMRSRLEGLVDAFPKLIGNEKEAATRQHTFVETDSV 60
Query: 56 RYVYKPFDDYYIILITNLHSNIIQDMDTLNIFVQTVDSTLKGLSEEDVFDSAFDILNSFD 115
RYVY P D+ Y++L+T +SNI++D++TL +FV+ + + E+++ FD++ +FD
Sbjct: 61 RYVYHPLDNIYLVLVTTKNSNILEDLETLRLFVRVIPEYCRSNEEKEILAHDFDLIFAFD 120
Query: 116 EIITMGYKENLSLTQIKTFLAMESHEERI-QEIIXXXXXXXXXXXXXXXXXXXXXXXQAR 174
E++T+GY+E+++L QI+TF M+SHEER+ +I +A
Sbjct: 121 EVVTLGYRESVNLAQIRTFTEMDSHEERVFMQIKEAQEKAAKQAMAEKAKELKRAQKEAL 180
Query: 175 KLGI-PDTQEFTGIHGGVMNKAXXXXXXXXXXXXXXXXXXXXXNXXXXXXXXXXXXXXXX 233
G+ P Q TGI A
Sbjct: 181 SRGLKPSYQSSTGISSSSTPNAAAVSEPAAPRPS-------------------------- 214
Query: 234 XYANREPL-GGKGLQVNGPRRGVERPIKNVERQ-----------------SLSVQTATLP 275
A + P+ GGK L++ G + + + +Q SL+ +T P
Sbjct: 215 --APKGPIGGGKALKLGGKTNNEDDFLDTLRQQGQSIAPVQKASLSGGVSSLAAPISTAP 272
Query: 276 EEEKPINNGILISVKETINAEISRDGAIMGSELKGVLELRVNNPDLAHAKLCLGTDVNVK 335
++ + + + +E IN +SRDG + E++ + L + +P+ + + N
Sbjct: 273 RVKREV---VHVRTEEKINTRVSRDGGLESGEVQATVTLSIGSPEFIPISIKMN---NGS 326
Query: 336 DKTYQFKTHPNVDKAGFLTNKTIGLRDPNKPFPSNDQSLGVLRWRKVKGADEHDLIPLEI 395
Q + HPN+DK + ++ T+ ++ KP+P N +G+L+W+ E + +P+
Sbjct: 327 AAGTQLQVHPNLDKKEWQSSSTLKIKPNGKPYPVN-SDVGILKWK--MALSEEEQLPISF 383
Query: 396 STWLSPSSETDNAIDVTFEYEVNSDYEGTIDELRFLIPVFTDTVFIKDDLNEANAIVEAI 455
+ W E+ + + V EY + + + T++ +R ++P+ T T + E + E
Sbjct: 384 NCW---PQESSDGVQVNIEYTLQRE-DITLNNVRIIVPLPTATA---PSVGECDGEYEYH 436
Query: 456 DESQGIIIKVDPIESGQSS-VFSVTVQAAYEDALFPIEVALKNSNAKPLSNLNIASVVSA 514
I+ + I+S SS +V + D FP+ V + N + + VV
Sbjct: 437 KTKNVIVWSLAVIDSSNSSGTLEFSVPNGHCDHFFPVSVGFTSENL--FVPITVQKVVK- 493
Query: 515 ADDTELPFDTVSTLKSEQYVVV 536
D + + + +T SE + +V
Sbjct: 494 NDGSPVTYSVETTFNSENFEIV 515
>SPCC285.08 [U] KOG2635 Medium subunit of clathrin adaptor complex
Length = 240
Score = 166 bits (420), Expect = 8e-41
Identities = 82/148 (55%), Positives = 114/148 (76%), Gaps = 1/148 (0%)
Query: 1 MVVLAVSITTRKGKPLLSRQFRDITKDRVMELLSNFQTLVANSSKQHTFVEDEHVRYVYK 60
MVVLAVSI R GK ++SRQFR++++ RV LLS+F LV+ S Q+T VE ++VR+VY+
Sbjct: 1 MVVLAVSIVNRGGKAIISRQFREMSRVRVESLLSSFPALVSEKS-QNTTVESDNVRFVYQ 59
Query: 61 PFDDYYIILITNLHSNIIQDMDTLNIFVQTVDSTLKGLSEEDVFDSAFDILNSFDEIITM 120
P D+ YI+LITNL SNI+QD+DTL++ Q V S L E ++ + AF+I +FDE ++
Sbjct: 60 PLDELYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSL 119
Query: 121 GYKENLSLTQIKTFLAMESHEERIQEII 148
GY++N+SLTQIKT+L MESHEE+IQEI+
Sbjct: 120 GYRDNVSLTQIKTYLEMESHEEKIQEIV 147
>At5g05010 [U] KOG2635 Medium subunit of clathrin adaptor complex
Length = 504
Score = 104 bits (260), Expect = 3e-22
Identities = 46/125 (36%), Positives = 87/125 (68%), Gaps = 1/125 (0%)
Query: 24 ITKDRVMELLSNFQTLVANSSKQHTFVEDEHVRYVYKPFDDYYIILITNLHSNIIQDMDT 83
+++ R+ LL+ F LV KQHT++E E+VRYVY+P + +++L+T SNI++D+ T
Sbjct: 1 MSRIRIEGLLAAFPKLVG-MGKQHTYIETENVRYVYQPIEALFLLLVTTKQSNILEDLAT 59
Query: 84 LNIFVQTVDSTLKGLSEEDVFDSAFDILNSFDEIITMGYKENLSLTQIKTFLAMESHEER 143
L + + V L EE + ++F+++ +FDE+I++G+KE++++ Q+K + MESHEE+
Sbjct: 60 LTLLSKLVPEYSMSLDEEGISRASFELIFAFDEVISLGHKESVTVAQVKQYCEMESHEEK 119
Query: 144 IQEII 148
+ +++
Sbjct: 120 LHKLV 124
Score = 81.6 bits (200), Expect = 3e-15
Identities = 68/248 (27%), Positives = 120/248 (47%), Gaps = 34/248 (13%)
Query: 260 KNVERQSLSVQTATLPEEEKPINNG----------ILISVKETINAEISRDGAIMGSELK 309
KN +SL + + E+ KP ++V+E +N + RDG + +++
Sbjct: 227 KNQLMESLKAEGEDVIEDVKPTGQSKAAAPPPTDPFTLTVEEKLNVALRRDGGLSSFDMQ 286
Query: 310 GVLELRVNNPDLAHAKLCLGTDVNVKDKTYQFKTHPNVDKAGFLTNKTIGLRDPNKPFPS 369
G L L++ N + ++ + T N + FKTHPN+++ F +GL+ P++PFP+
Sbjct: 287 GTLSLQILNQEDGFVQVQIATGENPE---ILFKTHPNINRDMFNNENILGLKRPDQPFPT 343
Query: 370 ND--QSLGVLRWRKVKGADEHDLIPLEISTWLSPSSETDNAIDVTFEYEVNSDYEGTIDE 427
+G+LRWR ++ ADE ++PL I+ W S S N V+ EYE +S ++ T
Sbjct: 344 GQGGDGVGLLRWR-MQRADE-SMVPLTINCWPSVSG---NETYVSLEYEASSMFDLTNVI 398
Query: 428 LRFLIPVFTDTVFIKDDLNE-----ANAIVE----AIDESQ---GIIIKVDPIESGQSSV 475
+ +P + ++ E N+++E ID S + V P++S S
Sbjct: 399 ISVPLPALREAPSVRQCDGEWRYDPRNSVLEWSILLIDNSNRSGSMEFVVPPVDS--SVF 456
Query: 476 FSVTVQAA 483
F ++VQ A
Sbjct: 457 FPISVQFA 464
>YPL259c [U] KOG0937 Adaptor complexes medium subunit family
Length = 475
Score = 52.8 bits (125), Expect = 1e-06
Identities = 34/140 (24%), Positives = 66/140 (46%), Gaps = 8/140 (5%)
Query: 5 AVSITTRKGKPLLSRQFRDITKDRVMELLSNFQTLVANSSKQHTFV----EDEHVRYVYK 60
AV GKPLLSR++RD D + + F L+++ +Q + + Y++
Sbjct: 4 AVYFCDHNGKPLLSRRYRD---DIPLSAIDKFPILLSDLEEQSNLIPPCLNHNGLEYLFI 60
Query: 61 PFDDYYII-LITNLHSNIIQDMDTLNIFVQTVDSTLKGLSEEDVFDSAFDILNSFDEIIT 119
+D Y++ ++T+L +N L+ V+ + LK + EE + D+ I DE++
Sbjct: 61 QHNDLYVVAIVTSLSANAAAIFTFLHKLVEVLSDYLKTVEEESIRDNFVIIYELLDEVMD 120
Query: 120 MGYKENLSLTQIKTFLAMES 139
G + +K ++ +S
Sbjct: 121 YGIPQITETKMLKQYITQKS 140
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.134 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,730,710
Number of Sequences: 60738
Number of extensions: 1078423
Number of successful extensions: 2259
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2238
Number of HSP's gapped (non-prelim): 13
length of query: 536
length of database: 30,389,216
effective HSP length: 111
effective length of query: 425
effective length of database: 23,647,298
effective search space: 10050101650
effective search space used: 10050101650
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)