ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2922 good O KOG0867 Posttranslational modification, protein turnover, chaperones Glutathione S-transferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2922 993485 994141 219
(219 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL048w_1 [O] KOG0867 Glutathione S-transferase 210 1e-54
YKL081w_1 [O] KOG0867 Glutathione S-transferase 147 1e-35
YGR201c [O] KOG0867 Glutathione S-transferase 145 6e-35
SPAC29A4.02c_1 [O] KOG0867 Glutathione S-transferase 87 2e-17
Hs4503481_1 [O] KOG0867 Glutathione S-transferase 75 5e-14
SPCC1183.02 [O] KOG0867 Glutathione S-transferase 75 7e-14
Hs20540877_1 [O] KOG0867 Glutathione S-transferase 74 1e-13
7301765_1 [O] KOG0867 Glutathione S-transferase 70 2e-12
At1g09640_1 [O] KOG0867 Glutathione S-transferase 58 9e-09
At2g30870 [O] KOG0867 Glutathione S-transferase 55 7e-08
CE05656_1 [O] KOG0867 Glutathione S-transferase 54 2e-07
At1g02920 [O] KOG0867 Glutathione S-transferase 50 2e-06
At2g30860 [O] KOG0867 Glutathione S-transferase 49 4e-06
>YPL048w_1 [O] KOG0867 Glutathione S-transferase
Length = 255
Score = 210 bits (535), Expect = 1e-54
Identities = 101/221 (45%), Positives = 149/221 (66%), Gaps = 3/221 (1%)
Query: 1 MSQGTLFANFRIRTWLPRAIVKSLSLDVKILEPESNVEYWSKLFPLKKVPCYVGSDGFKL 60
MSQGTL+ANFRIRTW+PR +VK+L LDVK++ P++ E +++ FPLKKVP +VG G+KL
Sbjct: 1 MSQGTLYANFRIRTWVPRGLVKALKLDVKVVTPDAAAEQFARDFPLKKVPAFVGPKGYKL 60
Query: 61 TQQLAILVYLVNLSDNNAEIQRLLGKGN--KEKAEVLQWLSLANTDLCLAFTRGAFPLNG 118
T+ +AI YLV LS ++ +LLG + +A++++W SLAN+DLC+ PL G
Sbjct: 61 TEAMAINYYLVKLSQDDKMKTQLLGADDDLNAQAQIIRWQSLANSDLCIQIANTIVPLKG 120
Query: 119 RSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADYLSAIIFTRIFETLYGKK 178
+PYNKK VD A +D++V +E RL ++ YL + SLAD ++A IFTR FE+L+G +
Sbjct: 121 GAPYNKKSVDSAMDAVDKIVDIFENRLKNYTYLATENISLADLVAASIFTRYFESLFGTE 180
Query: 179 WREEHAKLTQWFTNITEDPFVSFLWEGITLCDEPLPNPPTK 219
WR +H + +WF + PF+ ++ D+PL +PP K
Sbjct: 181 WRAQHPAIVRWFNTVRASPFLKDEYKDFKFADKPL-SPPQK 220
>YKL081w_1 [O] KOG0867 Glutathione S-transferase
Length = 253
Score = 147 bits (371), Expect = 1e-35
Identities = 80/219 (36%), Positives = 119/219 (53%), Gaps = 1/219 (0%)
Query: 1 MSQGTLFANFRIRTWLPRAIVKSLSLDVKILEPESNVEYWSKLFPLKKVPCYVGSDGFKL 60
MSQGTL+ N R + A++ LDVKI++ E + E+ S LFPLK+ P ++G G KL
Sbjct: 1 MSQGTLYINRSPRNYASEALISYFKLDVKIVDLEQSSEFAS-LFPLKQAPAFLGPKGLKL 59
Query: 61 TQQLAILVYLVNLSDNNAEIQRLLGKGNKEKAEVLQWLSLANTDLCLAFTRGAFPLNGRS 120
T+ LAI YL N + E RLLG EK+++L+W SLAN+D+ R G
Sbjct: 60 TEALAIQFYLANQVADEKERARLLGSDVIEKSQILRWASLANSDVMSNIARPFLSFKGLI 119
Query: 121 PYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADYLSAIIFTRIFETLYGKKWR 180
PYNKK VD + ++D L ++ RL D+ ++ + SL D +A + T+ G +WR
Sbjct: 120 PYNKKDVDACFVKIDNLAAVFDARLRDYTFVATENISLGDLHAAGSWAFGLATILGPEWR 179
Query: 181 EEHAKLTQWFTNITEDPFVSFLWEGITLCDEPLPNPPTK 219
+H L +WF + P V + + L ++ L P K
Sbjct: 180 AKHPHLMRWFNTVAASPIVKTPFAEVKLAEKALTYTPPK 218
>YGR201c [O] KOG0867 Glutathione S-transferase
Length = 274
Score = 145 bits (365), Expect = 6e-35
Identities = 80/218 (36%), Positives = 127/218 (57%), Gaps = 8/218 (3%)
Query: 1 MSQGTLFANFR----IRTWLPRAIVKSLSLDVKILEPESNVEYWSKLFPLKKVPCYVGS- 55
MS GTLF + + IRT +PR +V+SL LDVK+ +P + + + FPL+K P +VG
Sbjct: 50 MSDGTLFTDLKERKLIRTIVPRGLVRSLKLDVKLADPSDAQQLYEREFPLRKYPTFVGPH 109
Query: 56 DGFKLTQQLAILVYLVNLSDNNAEIQRLLGKGN--KEKAEVLQWLSLANTDLCLAFTRGA 113
D + LT+ +AI YL++LS + +++LLG K +A++L+W SL+N+D
Sbjct: 110 DEWTLTEAMAIDYYLIHLSSDKEAVRQLLGPEGDFKTRADILRWESLSNSDFLNEVCEVF 169
Query: 114 FPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLV-GDRASLADYLSAIIFTRIFE 172
FPL G PYN + A +D +V YE+RL +YLV D +LAD +SA F+ F
Sbjct: 170 FPLIGVKPYNATEFKAARENVDTIVSLYEKRLKKQQYLVCDDHETLADLISAAAFSLGFI 229
Query: 173 TLYGKKWREEHAKLTQWFTNITEDPFVSFLWEGITLCD 210
+ + + WR +H ++T+WF + + F +E +C+
Sbjct: 230 SFFDETWRSKHPEVTRWFNRVIKSRFFEGEFESFKMCE 267
>SPAC29A4.02c_1 [O] KOG0867 Glutathione S-transferase
Length = 253
Score = 87.0 bits (214), Expect = 2e-17
Identities = 60/217 (27%), Positives = 106/217 (48%), Gaps = 7/217 (3%)
Query: 1 MSQGTLFANF---RIRTWLPRAIVKSLSLDVKILEPESNVEYWSKLFPLKKVPCYVGSDG 57
MS GT++ R+ + A V + ++ ++P + + FPL+K+P +VG DG
Sbjct: 1 MSVGTVYGKIGSPRVLFCVSVAAVAGVEVEHVDVQPHNFPADLAAKFPLQKMPVFVGKDG 60
Query: 58 FKLTQQLAILVYLVNLSDNNAEIQRLLGKGNKEKAEVLQWLSLANTDLCLAFTRGAFPLN 117
F L++ LAI YL +L+ A L G +EKA+VLQ+ S N++L AF P
Sbjct: 61 FPLSETLAIAFYLASLNKTRA----LNGTTAEEKAKVLQYCSFTNSELPGAFRPIIAPRV 116
Query: 118 GRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADYLSAIIFTRIFETLYGK 177
+PY+++ +A + + R+++ L YLVG R +LAD + K
Sbjct: 117 FGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLTLADIFFTCFLKFGATYVLTK 176
Query: 178 KWREEHAKLTQWFTNITEDPFVSFLWEGITLCDEPLP 214
+ ++ + +++ I + + E + D+PLP
Sbjct: 177 SYLAKYTHIYRYYQTIYHQAKLDAITEPLKFIDQPLP 213
>Hs4503481_1 [O] KOG0867 Glutathione S-transferase
Length = 258
Score = 75.5 bits (184), Expect = 5e-14
Identities = 44/168 (26%), Positives = 79/168 (46%), Gaps = 8/168 (4%)
Query: 44 FPLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLLGKGNKEKAEVLQWLSLANT 103
FP KVP + G DGF + + AI Y+ N + L G + A+V+QW+S A++
Sbjct: 53 FPAGKVPAFEGDDGFCVFESNAIAYYVSN--------EELRGSTPEAAAQVVQWVSFADS 104
Query: 104 DLCLAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADYLS 163
D+ + FP G +NK+ ++A E+ R++ + L +LVG+R +LAD
Sbjct: 105 DIVPPASTWVFPTLGIMHHNKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITV 164
Query: 164 AIIFTRIFETLYGKKWREEHAKLTQWFTNITEDPFVSFLWEGITLCDE 211
+++ + +R+ +WF P + + LC++
Sbjct: 165 VCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEK 212
>SPCC1183.02 [O] KOG0867 Glutathione S-transferase
Length = 220
Score = 75.1 bits (183), Expect = 7e-14
Identities = 56/196 (28%), Positives = 97/196 (48%), Gaps = 8/196 (4%)
Query: 1 MSQGTLFA-NFRIRTWLPRAIVKSLSLDVKILE--PESNVEYWSKLFPLKKVPCYVGSDG 57
M GTL++ RT + K L L V ++E P + FPL+K+P ++G+DG
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPHKFSADLAAKFPLQKLPVFIGADG 60
Query: 58 FKLTQQLAILVYLVNLSDNNAEIQRLLGKGNK-EKAEVLQWLSLANTDLCLAFTRGAF-- 114
F+L++ +AI+ Y +N + LG N+ E+AE+L+W+ N D+ +
Sbjct: 61 FELSEVIAIVKYFYEKGKHND--KEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWVG 118
Query: 115 PLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADYLSAIIFTRIFETL 174
G PY +K ++ ++ + + D YLVGDR +LAD + F ++
Sbjct: 119 MFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNSI 178
Query: 175 YGKKWREEHAKLTQWF 190
+K R+E LT+++
Sbjct: 179 IDEKTRKELPHLTRYY 194
>Hs20540877_1 [O] KOG0867 Glutathione S-transferase
Length = 258
Score = 74.3 bits (181), Expect = 1e-13
Identities = 44/168 (26%), Positives = 78/168 (46%), Gaps = 8/168 (4%)
Query: 44 FPLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLLGKGNKEKAEVLQWLSLANT 103
FP KVP + G DGF + + AI Y+ N + L G + A+V+QW+S A++
Sbjct: 53 FPAGKVPAFEGDDGFCVFESNAIAYYVSN--------EELRGSTPEAAAQVVQWVSFADS 104
Query: 104 DLCLAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADYLS 163
D+ + FP G +NK+ ++A E+ R++ + L +LVG+R +LAD
Sbjct: 105 DIVPPASTWVFPTLGIMHHNKQATENAKEEVRRILGLLDAYLKTRTFLVGERVTLADITV 164
Query: 164 AIIFTRIFETLYGKKWREEHAKLTQWFTNITEDPFVSFLWEGITLCDE 211
+++ + +R +WF P + + LC++
Sbjct: 165 VCTLLWLYKQVLEPSFRRAFRNTNRWFLTCINQPQFRAVLGELKLCEK 212
>7301765_1 [O] KOG0867 Glutathione S-transferase
Length = 269
Score = 70.5 bits (171), Expect = 2e-12
Identities = 54/191 (28%), Positives = 85/191 (44%), Gaps = 10/191 (5%)
Query: 29 KILEPESNVEYWSKLFPLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLLGKGN 88
K E + E+ K FP KVP + ++G L++ AI L N Q GK
Sbjct: 35 KFGETNKSAEFLKK-FPGGKVPAFETAEGQYLSESNAIAYLLANE-------QLRGGKCP 86
Query: 89 KEKAEVLQWLSLANTDLCLAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDH 148
+A+V QW+S A+ ++ A FPL G P K A E + ++Q+ Q+L D
Sbjct: 87 FVQAQVQQWISFADNEIVPASCAWVFPLLGILPQQKNST--AKQEAEAVLQQLNQKLQDA 144
Query: 149 KYLVGDRASLADYLSAIIFTRIFETLYGKKWREEHAKLTQWFTNITEDPFVSFLWEGITL 208
+L G+R +LAD + ++E + R + +WF I V + + L
Sbjct: 145 TFLAGERITLADIVVFSSLLHLYEYVLEPSVRSAFGNVNRWFVTILNQKQVQAVVKDYKL 204
Query: 209 CDEPLPNPPTK 219
C++ L P K
Sbjct: 205 CEKALVFDPKK 215
>At1g09640_1 [O] KOG0867 Glutathione S-transferase
Length = 255
Score = 58.2 bits (139), Expect = 9e-09
Identities = 40/156 (25%), Positives = 71/156 (44%), Gaps = 5/156 (3%)
Query: 42 KLFPLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLLGKGNKEKAEVLQWLSLA 101
K+ P+ KVP +G + + AI Y+ L+ +N+ L G E A++ QW+ +
Sbjct: 47 KMNPIGKVPVLETPEG-SVFESNAIARYVSRLNGDNS----LNGSSLIEYAQIEQWIDFS 101
Query: 102 NTDLCLAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLADY 161
+ ++ + R P G PY+ + A + L R + L + YLVG +LAD
Sbjct: 102 SLEIYASILRWFGPRMGFMPYSAPAEEGAISTLKRALDALNTHLTSNTYLVGHSITLADI 161
Query: 162 LSAIIFTRIFETLYGKKWREEHAKLTQWFTNITEDP 197
++ F T+ KK+ E + ++F + P
Sbjct: 162 ITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQP 197
>At2g30870 [O] KOG0867 Glutathione S-transferase
Length = 215
Score = 55.1 bits (131), Expect = 7e-08
Identities = 47/176 (26%), Positives = 87/176 (48%), Gaps = 11/176 (6%)
Query: 25 SLDVKILEPESNVEYWSKLFPLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLL 84
+++V +++ E + + P K+P V D +K+ + AI+ Y+ ++ LL
Sbjct: 29 TVNVDLMKGEQRQPEYLAIQPFGKIPVLVDGD-YKIFESRAIMRYIAE--KYRSQGPDLL 85
Query: 85 GKGNKEKAEVLQWLSLANTD-----LCLAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQ 139
GK +E+ +V QWL + T L L PL G P ++K + ++ +L ++
Sbjct: 86 GKTIEERGQVEQWLDVEATSYHPPLLALTLNIVFAPLMG-FPADEKVIKESEEKLAEVLD 144
Query: 140 RYEQRLNDHKYLVGDRASLADYLSAIIFTRIFETLYGKKWR-EEHAKLTQWFTNIT 194
YE +L+ ++YL GD SLAD L+ + FT GK ++ ++ W+ I+
Sbjct: 145 VYEAQLSKNEYLAGDFVSLAD-LAHLPFTEYLVGPIGKAHLIKDRKHVSAWWDKIS 199
>CE05656_1 [O] KOG0867 Glutathione S-transferase
Length = 240
Score = 53.9 bits (128), Expect = 2e-07
Identities = 34/113 (30%), Positives = 51/113 (45%)
Query: 87 GNKEKAEVLQWLSLANTDLCLAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLN 146
G AE +QWL A L A P + ++KK V+ EL+ +Q ++ L
Sbjct: 66 GTSANAETVQWLQFAEGYLLPAVLGYVLPSVSAANFDKKTVEQYKNELNGQLQVLDRVLV 125
Query: 147 DHKYLVGDRASLADYLSAIIFTRIFETLYGKKWREEHAKLTQWFTNITEDPFV 199
YLVG+R SLAD A+ F+ + R+ +T+WF + P V
Sbjct: 126 KKTYLVGERLSLADVSVALDLLPAFQYVLDANARKSIVNVTRWFRTVVNQPAV 178
>At1g02920 [O] KOG0867 Glutathione S-transferase
Length = 209
Score = 50.4 bits (119), Expect = 2e-06
Identities = 41/158 (25%), Positives = 75/158 (46%), Gaps = 10/158 (6%)
Query: 45 PLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLLGKGNKEKAEVLQWLSLANTD 104
P KVP + D FKL + AI Y+ + + +L+ G+K+ A + + + + +
Sbjct: 51 PFGKVPAFEDGD-FKLFESRAITQYIAHFYSDKGN--QLVSLGSKDIAGIAMGIEIESHE 107
Query: 105 LC-----LAFTRGAFPLNGRSPYNKKQVDDAYAELDRLVQRYEQRLNDHKYLVGDRASLA 159
L + + PL G + +K V++ A+L +++ YE RL + KYL D+ +L
Sbjct: 108 FDPVGSKLVWEQVLKPLYGMTT-DKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLV 166
Query: 160 DYLSAIIFTRIFETLYGKKWREEHAKLTQWFTNITEDP 197
D + + + T KK +E ++ W +IT P
Sbjct: 167 DLHTIPVIQYLLGT-PTKKLFDERPHVSAWVADITSRP 203
>At2g30860 [O] KOG0867 Glutathione S-transferase
Length = 215
Score = 49.3 bits (116), Expect = 4e-06
Identities = 44/178 (24%), Positives = 83/178 (45%), Gaps = 9/178 (5%)
Query: 25 SLDVKILEPESNVEYWSKLFPLKKVPCYVGSDGFKLTQQLAILVYLVNLSDNNAEIQRLL 84
++ V +++ E + L P VP V D +K+ + A++ Y+ ++ LL
Sbjct: 29 TIPVDLMKGEHKQPAYLALQPFGTVPAVVDGD-YKIFESRAVMRYVAE--KYRSQGPDLL 85
Query: 85 GKGNKEKAEVLQWLSLANTDLCLAFTRGAFPLNGRS----PYNKKQVDDAYAELDRLVQR 140
GK +++ +V QWL + T + S P ++K + ++ +L ++
Sbjct: 86 GKTVEDRGQVEQWLDVEATTYHPPLLNLTLHIMFASVMGFPSDEKLIKESEEKLAGVLDV 145
Query: 141 YEQRLNDHKYLVGDRASLADYLSAIIFTRIFETLYGKKWR-EEHAKLTQWFTNITEDP 197
YE L+ KYL GD SLAD L+ + FT GK + ++ ++ W+ +I+ P
Sbjct: 146 YEAHLSKSKYLAGDFVSLAD-LAHLPFTDYLVGPIGKAYMIKDRKHVSAWWDDISSRP 202
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,854,863
Number of Sequences: 60738
Number of extensions: 562664
Number of successful extensions: 1537
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1517
Number of HSP's gapped (non-prelim): 13
length of query: 219
length of database: 30,389,216
effective HSP length: 102
effective length of query: 117
effective length of database: 24,193,940
effective search space: 2830690980
effective search space used: 2830690980
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)