ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV2949 good O KOG2817 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV2949 999198 1000385 396
(396 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR255c [O] KOG2817 Predicted E3 ubiquitin ligase 288 7e-78
SPBC29A3.03c [O] KOG2817 Predicted E3 ubiquitin ligase 96 7e-20
At4g37880 [O] KOG2817 Predicted E3 ubiquitin ligase 89 1e-17
At2g22690 [O] KOG2817 Predicted E3 ubiquitin ligase 87 6e-17
Hs21362098 [O] KOG2817 Predicted E3 ubiquitin ligase 82 1e-15
HsM12232433 [O] KOG2817 Predicted E3 ubiquitin ligase 81 2e-15
7291223 [O] KOG2817 Predicted E3 ubiquitin ligase 75 2e-13
At5g09630 [O] KOG2817 Predicted E3 ubiquitin ligase 74 4e-13
Hs12232469 [O] KOG2817 Predicted E3 ubiquitin ligase 72 1e-12
ECU04g1430 [O] KOG2817 Predicted E3 ubiquitin ligase 60 4e-09
CE29467 [O] KOG2817 Predicted E3 ubiquitin ligase 51 3e-06
>YDR255c [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 421
Score = 288 bits (738), Expect = 7e-78
Identities = 161/424 (37%), Positives = 229/424 (53%), Gaps = 32/424 (7%)
Query: 1 MSTLLETLDQQWVNSRLYVDKDERRTQLGKCVEEVHDFKIQXXXXXXXXXXX--GSSPQ- 57
MS LL++ + ++ ++ Y D + T L KC++ H+FK Q S P+
Sbjct: 1 MSELLDSFETEF--AKFYTDSNLEETNLQKCLDHTHEFKSQLKKLKAHLNKHIQESKPEV 58
Query: 58 --------------KRLVIREKLAKSHKNWDXXXXXXXXXXXXTYRKFSKACVKPLNEFQ 103
KR +I EKL+KS + WD +F+K+ + L EF
Sbjct: 59 YNKLSDKEKQKFKRKRELIIEKLSKSQRQWDHSVKKQIKYVSQQSNRFNKSTLNKLKEFD 118
Query: 104 LDSVYVNKINPQKLYYLDTAIFSHIARY-YTNMAVGTTSDEMLKYLHSTYRIPRSIAMDY 162
+DSVYVNK+ + + ++ AI HI RY NM +G +E +YL Y I + ++
Sbjct: 119 IDSVYVNKLPKETMENVNEAIGYHILRYSIDNMPLGN-KNEAFQYLKDVYGITNKESTEF 177
Query: 163 IEMANIVKEMRSGNLNTCIRWCQDNN---------LEVLHFQLHYLNAMSLLSENKPMEC 213
IEM IV +++ G+ +C++WC + L L F L+ L+AM ++ P+E
Sbjct: 178 IEMGQIVHDLKKGDTESCLKWCSNEMESLSSNHTALSSLKFDLYTLSAMQIVKHGNPVEL 237
Query: 214 YLYIQTNLASTVSKSSQLRMARPVSALLAKIVIGDTIPA--SKVDAYLETCVECFTKQYC 271
Y I N + + + + V LL K +IG I SKV+ L+ C F K+YC
Sbjct: 238 YYQITQNAPLDCFRHREKELMQNVVPLLTKSLIGQPIEDIDSKVNKELKECTSLFIKEYC 297
Query: 272 LQNKLPLHSALFLVVLSGVIGFQVFTKYEAIRKVSHVDWSTADELPFHVKLPPFLSNYHP 331
+ S LFL+VLSG+I FQ F KY+ IR+++HVDW+T DELPF VKLP FL+++HP
Sbjct: 298 AAKHIFFDSPLFLIVLSGLISFQFFIKYKTIRELAHVDWTTKDELPFDVKLPDFLTHFHP 357
Query: 332 IFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFKCPYCPVMSTKVKTKRVS 391
IFICPVLKEETT ENPPYSL CHH+ NGT FKCPYCPV ++ TK+V
Sbjct: 358 IFICPVLKEETTTENPPYSLACHHIISKKALDRLSKNGTITFKCPYCPVNTSMSSTKKVR 417
Query: 392 FMKL 395
F+ L
Sbjct: 418 FVML 421
>SPBC29A3.03c [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 398
Score = 96.3 bits (238), Expect = 7e-20
Identities = 46/127 (36%), Positives = 66/127 (51%), Gaps = 1/127 (0%)
Query: 266 FTKQYCLQNKLPLHSALFLVVLSGVIGFQVFTKYEAIRKVSHVDWSTADELPFHVKLPPF 325
F +++C + L S L +VV +G I + K +I K H +W++ ELP + LP
Sbjct: 273 FVREFCAALGMSLESPLDIVVNAGAIALPILLKMSSIMKKKHTEWTSQGELPVEIFLPSS 332
Query: 326 LSNYHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFKCPYCPVMSTKV 385
++H +F CPV KE+ T+ENPP + C HV NG+ FKCPYCP +
Sbjct: 333 Y-HFHSVFTCPVSKEQATEENPPMMMSCGHVIVKESLRQLSRNGSQRFKCPYCPNENVAA 391
Query: 386 KTKRVSF 392
RV F
Sbjct: 392 DAIRVYF 398
>At4g37880 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 388
Score = 89.0 bits (219), Expect = 1e-17
Identities = 63/233 (27%), Positives = 106/233 (45%), Gaps = 13/233 (5%)
Query: 157 SIAMDYIEMANIVKEMRSGNLNTCIRWCQDNNLEV------LHFQLHYLNAMSLLSENKP 210
S ++EM I++ M+ +L + W N+ ++ L +LH L+ + +
Sbjct: 146 STRQSFVEMYRILEAMKRRDLEPALNWAVSNSDKLKEARSDLEMKLHSLHFLEIARGKNS 205
Query: 211 MECYLYIQTNLASTVSKSSQLRMARPVSALLAKIVIGDTIPASKV--DAYLETCVECFTK 268
E Y + ++A T + S + + + +LL + D P S+ A V+ T+
Sbjct: 206 KEAIDYARKHIA-TFADSCLPEIQKLMCSLLWNRKL-DKSPYSEFLSPALWNNAVKELTR 263
Query: 269 QYCLQNKLPLHSALFLVVLSGVIGFQVFTKY-EAIRKVSHVDWSTADELPFHVKLPPFLS 327
QYC S L + V +G V KY + +DW T ++LP +L
Sbjct: 264 QYCNLLGESSESPLSITVTAGTQALPVLLKYMNVVMANKKLDWQTMEQLPVDAQLSEEFQ 323
Query: 328 NYHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGT-CNFKCPYCP 379
+H +F+CPV KE+++ +NPP + C HV NG+ +FKCPYCP
Sbjct: 324 -FHSVFVCPVSKEQSSDDNPPMMMSCGHVLCKQTINKMSKNGSKSSFKCPYCP 375
>At2g22690 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 381
Score = 86.7 bits (213), Expect = 6e-17
Identities = 60/244 (24%), Positives = 108/244 (43%), Gaps = 9/244 (3%)
Query: 157 SIAMDYIEMANIVKEMRSGNLNTCIRWCQDNNLEV------LHFQLHYLNAMSLLSENKP 210
S+ ++EM I++ M+ +L ++W N+ ++ L +LH L+ + + +
Sbjct: 139 SVTKAFMEMNMILEAMKERDLGPALKWVASNSDKLKEAKSDLELKLHSLHFLEIAKDKTS 198
Query: 211 MECYLYIQTNLASTVSKSSQLRMARPVSALLAKIVIGDTIPASKV--DAYLETCVECFTK 268
E Y + + A+ + S + + L I + P S+ + T+
Sbjct: 199 EEAINYARKHFATYSADSCCFPEIQKLMCSLLWIRNLNKSPYSEFLSPVLWTNAAKELTR 258
Query: 269 QYCLQNKLPLHSALFLVVLSGVIGFQVFTKYEAIRKVSHVDWSTADELPFHVKLPPFLSN 328
QYC+ S L + V +G F KY + +W T ++L V+L
Sbjct: 259 QYCILLGESPESPLSVTVAAGSQVLPTFLKYLNVLPEKRKEWQTMEQLLVPVELSEEY-R 317
Query: 329 YHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFKCPYCPVMSTKVKTK 388
++ +F+CPV KE ++++NPP L C HV NG+ +FKCPYCP + K
Sbjct: 318 FYSVFVCPVSKEHSSEDNPPMRLACGHVLCKQSINRMSRNGSRSFKCPYCPTDIDASQCK 377
Query: 389 RVSF 392
++ F
Sbjct: 378 QLYF 381
>Hs21362098 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 393
Score = 82.4 bits (202), Expect = 1e-15
Identities = 74/293 (25%), Positives = 127/293 (43%), Gaps = 29/293 (9%)
Query: 115 QKLYYLDTAIFSHIARYYTNMAVGTTSDEMLKYLHSTYRIPRSIAMDYIEMANIVKEMRS 174
Q+ L AI H+ Y M + ++E+ + ST + ++E+ I++ +
Sbjct: 115 QQQQILQMAIVEHL--YQQGML--SVAEELCQ--ESTLNVDLDFKQPFLELNRILEALHE 168
Query: 175 GNLNTCIRWCQDNNLEVLH------FQLHYLNAMSLLSEN--KPMECYLYIQ--TNLAST 224
+L + W + +L F+LH L+ + LL+ K +E Y + A
Sbjct: 169 QDLGPALEWAVSHRQRLLELNSSLEFKLHRLHFIRLLAGGPAKQLEALSYARHFQPFARL 228
Query: 225 VSKSSQLRMARPVSALLAKIVIGDTIPASKVDA--YLETCVECFTKQYCLQNKLPLHSAL 282
+ Q+ M S + ++ + + +D+ + E C E FT+ C L + S L
Sbjct: 229 HQREIQVMMG---SLVYLRLGLEKSPYCHLLDSSHWAEIC-ETFTRDACSLLGLSVESPL 284
Query: 283 FLVVLSGVIGFQVFTKYEAI---RKVSHVDWSTADELPFHVKLPPFLSNYHPIFICPVLK 339
+ SG + V +A+ R+ + V W+ DELP ++L YH +F CP+L+
Sbjct: 285 SVSFASGCVALPVLMNIKAVIEQRQCTGV-WNHKDELPIEIELG-MKCWYHSVFACPILR 342
Query: 340 EETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFKCPYCPVMSTKVKTKRVSF 392
++T+ NPP L C HV G KCPYCP+ KR+ F
Sbjct: 343 QQTSDSNPPIKLICGHVISRDALNKLINGG--KLKCPYCPMEQNPADGKRIIF 393
>HsM12232433 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 272
Score = 81.3 bits (199), Expect = 2e-15
Identities = 66/258 (25%), Positives = 112/258 (42%), Gaps = 23/258 (8%)
Query: 150 STYRIPRSIAMDYIEMANIVKEMRSGNLNTCIRWCQDNNLEVLH------FQLHYLNAMS 203
ST + ++E+ I++ + +L + W + +L F+LH L+ +
Sbjct: 23 STLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVSHRQRLLELNSSLEFKLHRLHFIR 82
Query: 204 LLSEN--KPMECYLYIQ--TNLASTVSKSSQLRMARPVSALLAKIVIGDTIPASKVDA-- 257
LL+ K +E Y + A + Q+ M S + ++ + + +D+
Sbjct: 83 LLAGGPAKQLEALSYARHFQPFARLHQREIQVMMG---SLVYLRLGLEKSPYCHLLDSSH 139
Query: 258 YLETCVECFTKQYCLQNKLPLHSALFLVVLSGVIGFQVFTKYEAI---RKVSHVDWSTAD 314
+ E C E FT+ C L + S L + SG + V +A+ R+ + V W+ D
Sbjct: 140 WAEIC-ETFTRDACSLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCTGV-WNHKD 197
Query: 315 ELPFHVKLPPFLSNYHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFK 374
ELP ++L YH +F CP+L+++T+ NPP L C HV G K
Sbjct: 198 ELPIEIELG-MKCWYHSVFACPILRQQTSDSNPPIKLICGHVISRDALNKLINGG--KLK 254
Query: 375 CPYCPVMSTKVKTKRVSF 392
CPYCP+ KR+ F
Sbjct: 255 CPYCPMEQNPADGKRIIF 272
>7291223 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 432
Score = 74.7 bits (182), Expect = 2e-13
Identities = 60/248 (24%), Positives = 106/248 (42%), Gaps = 24/248 (9%)
Query: 161 DYIEMANIVKEMRSGNLNTCIRWCQ------DNNLEVLHFQLHYLNAMSLLSE--NKPME 212
++ ++ I +++ +L ++W + + ++ F+LH + M L+S + E
Sbjct: 193 EFADIYGIWVKIQKRDLTDALKWAKMYSQQLSDRHSLIEFRLHRMRFMQLVSYGLDSQRE 252
Query: 213 CYLYIQTNLASTVSKSSQLRMARPVSALLAKIV-IGDTIPASKVDAYL--ETCVEC---F 266
Y + N K +R ++ L+A + + + S +L E E F
Sbjct: 253 AISYARLNF-----KKFAVRYEHEIANLMASFIYLPSGLENSPYKHFLGQEKWTELSFIF 307
Query: 267 TKQYCLQNKLPLHSALFLVVLSGVIGFQVFTKYEAIRKVSHV--DWSTADELPFHVKLPP 324
K C + +SAL +VV +G + + + V W+ DELP + L P
Sbjct: 308 LKDACQLLGISKNSALSVVVNAGCTALPALLNIKQVMQSRQVLGMWNGCDELPIEIDLQP 367
Query: 325 FLSNYHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFKCPYCPVMSTK 384
+H IF CP+L+++T+++NPP L C HV KCPYCPV
Sbjct: 368 EF-RFHSIFACPILRQQTSEDNPPKKLTCGHVISNDALHKLSVGHI--LKCPYCPVEQNA 424
Query: 385 VKTKRVSF 392
+ R+ F
Sbjct: 425 EEAVRIYF 432
>At5g09630 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 386
Score = 73.9 bits (180), Expect = 4e-13
Identities = 53/246 (21%), Positives = 104/246 (41%), Gaps = 17/246 (6%)
Query: 158 IAMDYIEMANIVKEMRSGNLNTCIRWCQDNNLEV------LHFQLHYLNAMSLLSENKPM 211
+ ++E IV ++ N+ +RW N ++ L F+L L +L E K
Sbjct: 147 VRSQFLEFHQIVDSLKLRNIEPAMRWIFANRGKLKQKSSKLEFKLLSLKYCDILREGKSD 206
Query: 212 ECYLYIQTNLASTVSKSSQLRMARPVSALLAKIVIGDTIPASKVDAYLETCVECFTKQYC 271
+ Y +T+ +++ + + IG+ + + +C + TK+
Sbjct: 207 DALEYARTHFTQYPLHFKEIQ-----KLITCLLWIGNFEKSPYAEIVSPSCWDKVTKELI 261
Query: 272 LQNK----LPLHSALFLVVLSGVIGFQVFTKYEAIRKVSHVDWSTADELPFHVKLPPFLS 327
++ P++S L + + +G K + ++ +W +LP ++L
Sbjct: 262 MEYHHLLDQPINSPLKVALSAGYESLPSLLKLVHLMALTKQEWQAMKQLPVPLELGNEYK 321
Query: 328 NYHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXN-GTCNFKCPYCPVMSTKVK 386
+H F+CPV ++++++ENPP LPC HV N FKCPYCP +
Sbjct: 322 -FHSAFVCPVSRDQSSEENPPMQLPCGHVISKQSMMRLSKNCAFRTFKCPYCPAETLASA 380
Query: 387 TKRVSF 392
+++ F
Sbjct: 381 CRQLYF 386
>Hs12232469 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 391
Score = 72.4 bits (176), Expect = 1e-12
Identities = 65/244 (26%), Positives = 103/244 (41%), Gaps = 19/244 (7%)
Query: 162 YIEMANIVKEMRSGNLNTCIRWCQDNNLEV------LHFQLHYLNAMSLL--SENKPMEC 213
++E+ I++ ++ L + W N + L F+LH L +SLL E
Sbjct: 154 FVELNRILEALKVRVLRPALEWAVSNREMLIAQNSSLEFKLHRLYFISLLMGGTTNQREA 213
Query: 214 YLYIQT--NLASTVSKSSQLRMARPVSALLAKIVIGDTIPASKVDAYLETCVECFTKQYC 271
Y + A K Q+ M V L I + + + + C + FT+ C
Sbjct: 214 LQYAKNFQPFALNHQKDIQVLMGSLVY-LRQGIENSPYVHLLDANQWADIC-DIFTRDAC 271
Query: 272 LQNKLPLHSALFLVVLSGVIGFQVFTKYEAI---RKVSHVDWSTADELPFHVKLPPFLSN 328
L + S L + +G + +A+ R+ + V W+ DELP V L
Sbjct: 272 ALLGLSVESPLSVSFSAGCVALPALINIKAVIEQRQCTGV-WNQKDELPIEVDLGKKCW- 329
Query: 329 YHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCNFKCPYCPVMSTKVKTK 388
YH IF CP+L+++TT NPP L C H+ NG+ KCPYCP+ + K
Sbjct: 330 YHSIFACPILRQQTTDNNPPMKLVCGHI-ISRDALNKMFNGS-KLKCPYCPMEQSPGDAK 387
Query: 389 RVSF 392
++ F
Sbjct: 388 QIFF 391
>ECU04g1430 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 336
Score = 60.5 bits (145), Expect = 4e-09
Identities = 42/121 (34%), Positives = 54/121 (43%), Gaps = 7/121 (5%)
Query: 263 VECFTKQYCLQNKLPLHSALFLVVLSGVIGFQVFTKYEAIRKVSHVDWSTADELPFHVKL 322
+E F +Y N +P L + +G F + ++ + D ST LP +K+
Sbjct: 212 IEKFKSKYIEMNGMPNRCYLRELFETGTSAFLQLSTSGSL--LFDKDDST---LPMEIKI 266
Query: 323 PPFLSNYHPIFICPVLKEETTKENPPYSLPCHHVXXXXXXXXXXXNGTCN-FKCPYCPVM 381
NYH +FICPVLK EN P L C HV G N FKCPYCP M
Sbjct: 267 EKG-RNYHSLFICPVLKTLCVDENIPVMLECGHVISLEAASVLSQEGVLNSFKCPYCPEM 325
Query: 382 S 382
S
Sbjct: 326 S 326
>CE29467 [O] KOG2817 Predicted E3 ubiquitin ligase
Length = 432
Score = 51.2 bits (121), Expect = 3e-06
Identities = 26/73 (35%), Positives = 32/73 (43%), Gaps = 8/73 (10%)
Query: 330 HPIFICPVLKEETTKENPPYSLPCHHV--------XXXXXXXXXXXNGTCNFKCPYCPVM 381
H F CP+LKE+ ENPP L C HV + FKCPYCP
Sbjct: 342 HSSFTCPILKEQCDAENPPMRLICGHVISKDAINRLTTSIRQQRNSSRLSKFKCPYCPRE 401
Query: 382 STKVKTKRVSFMK 394
T++V F+K
Sbjct: 402 QLLENTRQVDFLK 414
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.135 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,793,518
Number of Sequences: 60738
Number of extensions: 843097
Number of successful extensions: 1769
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1749
Number of HSP's gapped (non-prelim): 11
length of query: 396
length of database: 30,389,216
effective HSP length: 108
effective length of query: 288
effective length of database: 23,829,512
effective search space: 6862899456
effective search space used: 6862899456
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)