ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3001 good K KOG2240 Transcription RNA polymerase II general transcription factor BTF3 and related proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3001 1016927 1016454 -158
(158 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL037c [K] KOG2240 RNA polymerase II general transcription fact... 173 8e-44
YDR252w [K] KOG2240 RNA polymerase II general transcription fact... 101 5e-22
Hs16159637 [K] KOG2240 RNA polymerase II general transcription f... 71 7e-13
7303391 [K] KOG2240 RNA polymerase II general transcription fact... 67 1e-11
Hs20070130 [K] KOG2240 RNA polymerase II general transcription f... 63 1e-10
Hs22051950 [K] KOG2240 RNA polymerase II general transcription f... 62 3e-10
At1g73230 [K] KOG2240 RNA polymerase II general transcription fa... 61 7e-10
At1g17880 [K] KOG2240 RNA polymerase II general transcription fa... 60 9e-10
SPAC4F10.14c [K] KOG2240 RNA polymerase II general transcription... 59 4e-09
7296189_1 [K] KOG2240 RNA polymerase II general transcription fa... 58 6e-09
Hs17463721 [K] KOG2240 RNA polymerase II general transcription f... 57 8e-09
CE02573 [K] KOG2240 RNA polymerase II general transcription fact... 57 1e-08
Hs22044784 [K] KOG2240 RNA polymerase II general transcription f... 54 9e-08
Hs22054463 [K] KOG2240 RNA polymerase II general transcription f... 52 3e-07
>YPL037c [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 157
Score = 173 bits (439), Expect = 8e-44
Identities = 95/161 (59%), Positives = 106/161 (65%), Gaps = 7/161 (4%)
Query: 1 MPIDQEKLAKLQKLSANNKVGGTRRKF---AXXXXXXXXXXXXXXXXXXXXRAVTVDNVQ 57
MPIDQEKLAKLQKLSANNKVGGTRRK A AVT+DNV
Sbjct: 1 MPIDQEKLAKLQKLSANNKVGGTRRKLNKKAGSSAGANKDDTKLQSQLAKLHAVTIDNVA 60
Query: 58 QANFFKDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQ 117
+ANFFKDDG V+HFNKVGVQVA QHNTSVFYG+PQEK+LQ+LFP II Q+G +AI AL+Q
Sbjct: 61 EANFFKDDGKVMHFNKVGVQVAAQHNTSVFYGLPQEKNLQDLFPGIISQLGPEAIQALSQ 120
Query: 118 MXXXXXXXXXXXXXXXXXXXXXKDIDIPELVEGQTFEADVE 158
+ KD IPELVEGQTF+ADVE
Sbjct: 121 L----AAQMEKHEAKAPADAEKKDEAIPELVEGQTFDADVE 157
>YDR252w [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 149
Score = 101 bits (251), Expect = 5e-22
Identities = 53/119 (44%), Positives = 70/119 (58%), Gaps = 3/119 (2%)
Query: 1 MPIDQEKLAKLQKLSANNKVGGTRRKF---AXXXXXXXXXXXXXXXXXXXXRAVTVDNVQ 57
MP+DQEKLAKL KLSA NKVGGTRRK +T++NV
Sbjct: 1 MPVDQEKLAKLHKLSAANKVGGTRRKINKKGNLYNNNDKDNTKLQAELHKLHPMTIENVA 60
Query: 58 QANFFKDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALT 116
+ANFFK +G VLHFN VQ+APQ N ++ +G P+E +L L+P + Q+G+ + LT
Sbjct: 61 EANFFKKNGKVLHFNSAVVQIAPQCNLTMIHGQPKENTLNGLYPSVASQLGSQELEYLT 119
>Hs16159637 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 158
Score = 70.9 bits (172), Expect = 7e-13
Identities = 42/147 (28%), Positives = 63/147 (42%)
Query: 3 IDQEKLAKLQKLSANNKVGGTRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFF 62
++QEKLAKLQ G RRK + +++ N
Sbjct: 1 MNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQSSLKKLAVNNIAGIEEVNMI 60
Query: 63 KDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXX 122
KDDGTV+HFN VQ + NT G + K + E+ P I+ Q+GAD++ +L ++
Sbjct: 61 KDDGTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTSLRKLAEQF 120
Query: 123 XXXXXXXXXXXXXXXXXKDIDIPELVE 149
+D D+P+LVE
Sbjct: 121 PRQVLDSKAPKPEDIDEEDDDVPDLVE 147
>7303391 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 169
Score = 67.0 bits (162), Expect = 1e-11
Identities = 39/143 (27%), Positives = 59/143 (40%), Gaps = 3/143 (2%)
Query: 10 KLQKLSANNKVGGT---RRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFFKDDG 66
KL+KL A ++GG RRK T+ +++ N K+DG
Sbjct: 5 KLKKLQAQVRIGGKGTPRRKKKIVHSTPATDDKKLQSSLKKLSVNTIPGIEEVNIIKNDG 64
Query: 67 TVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXXXXXX 126
TV+HFN Q + NT G + K++ E+ P I+ Q+G IN L ++
Sbjct: 65 TVIHFNNPKAQASLPTNTFAITGHGENKTITEMVPGILTQLGPQDINQLKKLATEIASKS 124
Query: 127 XXXXXXXXXXXXXKDIDIPELVE 149
D D+P+LVE
Sbjct: 125 GAGGAAGSSAADAGDDDVPDLVE 147
>Hs20070130 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 162
Score = 63.2 bits (152), Expect = 1e-10
Identities = 40/147 (27%), Positives = 61/147 (41%), Gaps = 1/147 (0%)
Query: 3 IDQEKLAKLQKLSANNKVGGTRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFF 62
++QEKLAKLQ G RRK + +++ N F
Sbjct: 6 MNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMF 65
Query: 63 KDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXX 122
+ GTV+HFN VQ + NT G + K L E+ P I+ Q+GAD++ +L ++
Sbjct: 66 TNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEAL 125
Query: 123 XXXXXXXXXXXXXXXXXKDIDIPELVE 149
D ++P+LVE
Sbjct: 126 PKQSVDGKAPLATGEDDDD-EVPDLVE 151
>Hs22051950 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 156
Score = 62.0 bits (149), Expect = 3e-10
Identities = 35/116 (30%), Positives = 53/116 (45%)
Query: 3 IDQEKLAKLQKLSANNKVGGTRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFF 62
++QEKLAKLQ G RRK + +++ N F
Sbjct: 6 MNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMF 65
Query: 63 KDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQM 118
+ GTV+HFN VQ + NT G + K L E+ P I+ Q+GAD++ +L ++
Sbjct: 66 TNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRL 121
>At1g73230 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 165
Score = 60.8 bits (146), Expect = 7e-10
Identities = 42/153 (27%), Positives = 63/153 (40%), Gaps = 2/153 (1%)
Query: 3 IDQEKLAKLQKLSANNKVGGTRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFF 62
+++EKL K+ G RRK ++ +++ N F
Sbjct: 1 MNREKLMKMANTVRTGGKGTVRRKKKAVHKTTTTDDKRLQSTLKRVGVNSIPAIEEVNIF 60
Query: 63 KDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXX 122
KDD V+ F VQ + NT V G PQ K LQ++ P II Q+G D ++ L ++
Sbjct: 61 KDD-VVIQFINPKVQASIAANTWVVSGTPQTKKLQDILPQIISQLGPDNLDNLKKLAEQF 119
Query: 123 XXXX-XXXXXXXXXXXXXKDIDIPELVEGQTFE 154
D D+P+LV G+TFE
Sbjct: 120 QKQAPGAGDVPATIQEEDDDDDVPDLVVGETFE 152
>At1g17880 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 165
Score = 60.5 bits (145), Expect = 9e-10
Identities = 44/157 (28%), Positives = 65/157 (41%), Gaps = 3/157 (1%)
Query: 3 IDQEKLAKLQKLSANNKVGGTRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFF 62
+++EKL K+ G RRK ++ +++ N F
Sbjct: 1 MNREKLMKMANTVRTGGKGTVRRKKKAVHKTNTTDDKRLQSTLKRIGVNSIPAIEEVNIF 60
Query: 63 KDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXX 122
KDD V+ F VQ + NT V G PQ K LQ++ P II Q+G D ++ L ++
Sbjct: 61 KDD-VVIQFINPKVQASIAANTWVVSGSPQTKKLQDILPQIISQLGPDNMDNLKKLAEQF 119
Query: 123 XXXXXXXXXXXXXXXXXK-DIDIPELVEGQTFEADVE 158
+ D D+PELV G+TFE E
Sbjct: 120 QKQASGEGNAASATIQEEDDDDVPELV-GETFETAAE 155
>SPAC4F10.14c [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 151
Score = 58.5 bits (140), Expect = 4e-09
Identities = 42/152 (27%), Positives = 67/152 (43%), Gaps = 12/152 (7%)
Query: 3 IDQEKLAKLQKLSANNKVGG--TRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDN---VQ 57
+D KLAKLQ A ++GG T R+ + + + N +Q
Sbjct: 1 MDPSKLAKLQ---AGARIGGKGTPRRKVKKPSKSAMSAADDKKVQGALKKLNMQNLAGIQ 57
Query: 58 QANFFKDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQ 117
+ N FK+DG V++F V + + T+ YG +EK+L E+ P I+ +G +++ AL Q
Sbjct: 58 EVNMFKEDGGVINFRAPTVHSSLPNETTAIYGKAEEKTLSEILPGILNNLGPESLTALRQ 117
Query: 118 MXXXXXXXXXXXXXXXXXXXXXKDIDIPELVE 149
M D +IP+LVE
Sbjct: 118 M----AEQLKVSEGEKGADAQADDGEIPDLVE 145
>7296189_1 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 286
Score = 57.8 bits (138), Expect = 6e-09
Identities = 36/142 (25%), Positives = 56/142 (39%), Gaps = 3/142 (2%)
Query: 10 KLQKLSANNKVGGT---RRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFFKDDG 66
KL++L A ++GG RRK T+ +++ N KDD
Sbjct: 5 KLKRLQAQVRIGGKGTPRRKKKVMHQTAATDDKKLQSSLKKLSVSTIPGIEEVNIIKDDL 64
Query: 67 TVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXXXXXX 126
TV+HFN Q + NT G + + + E+ PDI+PQ+G + + L
Sbjct: 65 TVIHFNNPKAQASLSANTFAVTGHGETRKVVEMLPDILPQLGQETVVQLRMYANAMNSQK 124
Query: 127 XXXXXXXXXXXXXKDIDIPELV 148
+D D+P LV
Sbjct: 125 GAPGSGDGPLPAEEDDDVPLLV 146
>Hs17463721 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 162
Score = 57.4 bits (137), Expect = 8e-09
Identities = 38/146 (26%), Positives = 58/146 (39%), Gaps = 1/146 (0%)
Query: 4 DQEKLAKLQKLSANNKVGGTRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFFK 63
+QEKLAKLQ G RK + +++ N F
Sbjct: 7 NQEKLAKLQAQVCIGGKGPACRKKKVVHRTATAEDKNFQFSLKKLGVNNISGIEEVNMFT 66
Query: 64 DDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXXX 123
+ GTV+HFN VQ + NT G + K L E+ P I+ Q+G D++ +L ++
Sbjct: 67 NQGTVIHFNNPEVQTSLAGNTFTITGHAKTKQLTEMLPSILIQLGVDSLASLRRLAEALP 126
Query: 124 XXXXXXXXXXXXXXXXKDIDIPELVE 149
D ++P+LVE
Sbjct: 127 KQSVDGTAPFATGEDDDD-EVPDLVE 151
>CE02573 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 161
Score = 57.0 bits (136), Expect = 1e-08
Identities = 37/146 (25%), Positives = 59/146 (40%), Gaps = 11/146 (7%)
Query: 6 EKLAKLQKLSANNKVGGT---RRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFF 62
E++ KLQ + ++GG RRK + +++ N
Sbjct: 8 ERIKKLQAQQEHVRIGGKGTPRRKKKVIHKTAAADDKKLQSNLKKLSVTNIPGIEEVNMI 67
Query: 63 KDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQMXXXX 122
KDDGTV+HFN VQ + NT G K + E+ P I+ Q+G +++ L ++
Sbjct: 68 KDDGTVIHFNNPKVQTSVPANTFSVTGSADNKQITEMLPGILNQLGPESLTHLKKL---- 123
Query: 123 XXXXXXXXXXXXXXXXXKDIDIPELV 148
+D D+PELV
Sbjct: 124 ----ANNVTKLGPDGKGEDEDVPELV 145
>Hs22044784 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 440
Score = 53.9 bits (128), Expect = 9e-08
Identities = 29/112 (25%), Positives = 50/112 (43%), Gaps = 3/112 (2%)
Query: 10 KLQKLSANNKVGG---TRRKFAXXXXXXXXXXXXXXXXXXXXRAVTVDNVQQANFFKDDG 66
KL KL A ++GG RK + + +++ N F + G
Sbjct: 266 KLAKLQAQVRIGGKGTAHRKKKVFHRTATADDKKLQFSLKKLQVNNISGIEKVNMFTNQG 325
Query: 67 TVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINALTQM 118
TV+HFN Q + NT G + K + E+ P ++ Q+GAD++ +L ++
Sbjct: 326 TVIHFNNPKFQASLAVNTFTITGHAEAKQVTEMLPSVLSQLGADSLTSLRRL 377
>Hs22054463 [K] KOG2240 RNA polymerase II general transcription factor BTF3 and
related proteins
Length = 366
Score = 52.4 bits (124), Expect = 3e-07
Identities = 23/63 (36%), Positives = 37/63 (58%)
Query: 56 VQQANFFKDDGTVLHFNKVGVQVAPQHNTSVFYGIPQEKSLQELFPDIIPQMGADAINAL 115
+++ N KDD TV+HFN VQ + NT G + K + E+ P I+ Q+GAD++ L
Sbjct: 262 IEEVNMIKDDRTVIHFNNPKVQASLSANTFAITGHAEAKPITEMLPGILSQLGADSLTIL 321
Query: 116 TQM 118
++
Sbjct: 322 KKL 324
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,420,181
Number of Sequences: 60738
Number of extensions: 172340
Number of successful extensions: 347
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 328
Number of HSP's gapped (non-prelim): 14
length of query: 158
length of database: 30,389,216
effective HSP length: 97
effective length of query: 61
effective length of database: 24,497,630
effective search space: 1494355430
effective search space used: 1494355430
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)