ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3062 good E KOG0564 Amino acid transport and metabolism 5,10-methylenetetrahydrofolate reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3062 1037537 1035591 -649
(649 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL023c [E] KOG0564 510-methylenetetrahydrofolate reductase 770 0.0
SPAC343.10 [E] KOG0564 510-methylenetetrahydrofolate reductase 474 e-133
SPAC56F8.10 [E] KOG0564 510-methylenetetrahydrofolate reductase 397 e-110
Hs6631098 [E] KOG0564 510-methylenetetrahydrofolate reductase 198 2e-50
YGL125w [E] KOG0564 510-methylenetetrahydrofolate reductase 193 6e-49
At2g44160 [E] KOG0564 510-methylenetetrahydrofolate reductase 179 2e-44
At3g59970 [E] KOG0564 510-methylenetetrahydrofolate reductase 177 6e-44
CE02451 [E] KOG0564 510-methylenetetrahydrofolate reductase 151 3e-36
>YPL023c [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 657
Score = 770 bits (1988), Expect = 0.0
Identities = 386/655 (58%), Positives = 465/655 (70%), Gaps = 19/655 (2%)
Query: 12 VTVKQRFDELKGPCVSLEFFPPKTEPGKRNLLARMERMCALNPLFIXXXXXXXXXXXXXX 71
++++ + P +SLEFFPPKTE G RNL+ RM RM AL+PLFI
Sbjct: 1 MSIRDLYHARASPFISLEFFPPKTELGTRNLMERMHRMTALDPLFITVTWGAGGTTAEKT 60
Query: 72 XXXXXIAQRELNVPVCMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEW--S 129
+AQ+ LN+PVCMHLTCTN K IID+AL +A IRNILALRGDP IGE+W S
Sbjct: 61 LTLASLAQQTLNIPVCMHLTCTNTEKAIIDDALDRCYNAGIRNILALRGDPPIGEDWLDS 120
Query: 130 PDADTEFKYAVDLVRYIKKNYGDDFCVGVAAYPEGHCEGEADESLQDPMRDLPYLKEKVD 189
++ FKYAVDLVRYIK++YGD FCVGVAAYPEGHCEGEA+ QDP++DL YLKEKV+
Sbjct: 121 QSNESPFKYAVDLVRYIKQSYGDKFCVGVAAYPEGHCEGEAEGHEQDPLKDLVYLKEKVE 180
Query: 190 AGADFIITQLFYDVDKFLSFEKLVREKVSSDIPMFPGLMPINSHLLFNRAAKLSHASIPQ 249
AGADF+ITQLFYDV+KFL+FE L RE++S D+P+FPGLMPINS+LLF+RAAKLSHASIP
Sbjct: 181 AGADFVITQLFYDVEKFLTFEMLFRERISQDLPLFPGLMPINSYLLFHRAAKLSHASIPP 240
Query: 250 YILDRFPASVQSDDNRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIVAK 309
IL RFP +QSDDN VK +T+GRIK FHFYTLNLE+AIAQIV++
Sbjct: 241 AILSRFPPEIQSDDNAVKSIGVDILIELIQEIYQRTSGRIKGFHFYTLNLEKAIAQIVSQ 300
Query: 310 SPTLSKILXXXXXXXXXXXXXXXXXXXX---------------XXXXXXXNIKKRKRQSS 354
SP LS I+ N K+R+ S
Sbjct: 301 SPVLSHIVNESSEEEGEDETSGEIGSIENVPIEDADGDIVLDDSNEETVANRKRRRHSSL 360
Query: 355 ASTDFPLNTSIINKLNGMSHGNRVPSVPSRKVMISISQGTGVLGRNVTWDEFPNGRFGDS 414
S N +I+ + G+ + N S+PS+K +ISIS+G G LGR+ TWDEFPNGRFGDS
Sbjct: 361 DSAKLIFNRAIVTE-KGLRYNNENGSMPSKKALISISKGHGTLGRDATWDEFPNGRFGDS 419
Query: 415 RSPAYGEIDGYGPSLKVCNSTAYELWDHPVSLEDIKNIFIRYLEGSMKALPWSDLGLSAE 474
RSPAYGEIDGYGPS+KV S A ELW P ++ D+K+IFI+YLEGS A+PWSDLGLSAE
Sbjct: 420 RSPAYGEIDGYGPSIKVSKSKALELWGIPKTIGDLKDIFIKYLEGSTDAIPWSDLGLSAE 479
Query: 475 TAFIQEELIELNQRGYLSLASQPATNGSQSTDNIFGWGPANGLIYQKAFVEIFVRKEEWN 534
TA IQEELI+LN RGYL+LASQPATN + S+D IFGWGPA G +YQKAFVE+F+ +++W
Sbjct: 480 TALIQEELIQLNYRGYLTLASQPATNATLSSDKIFGWGPAKGRLYQKAFVEMFIHRQQWE 539
Query: 535 QVLKPKIES-KNGEFSYYLGDYTGYFETNLQPKSSNAVTWGVFPNSEVVQTTIVEEESFK 593
LKPK++ +FSYY GD +G FETNL P SS+ VTWGVFPNS V QTTI+EEESFK
Sbjct: 540 TTLKPKLDHYGRRKFSYYAGDSSGSFETNLDPHSSSVVTWGVFPNSPVKQTTIIEEESFK 599
Query: 594 AWRDEAFSIWIEWSKLFPKNTPENTFLRKMHRDYYLVSIVHHDFINPDGLWDLLL 648
AWRDEAFSIW EW+KLFP+NTP N LR +H+DY LVSIVHHDF D LW++LL
Sbjct: 600 AWRDEAFSIWSEWAKLFPRNTPANILLRLVHKDYCLVSIVHHDFKETDELWEMLL 654
>SPAC343.10 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 641
Score = 474 bits (1220), Expect = e-133
Identities = 250/629 (39%), Positives = 362/629 (56%), Gaps = 17/629 (2%)
Query: 27 SLEFFPPKTEPGKRNLLARMERMCA-LNPLFIXXXXXXXXXXXXXXXXXXXIAQRELNVP 85
S E+FPPKT G NL +R+ RM + PLF + ++ +P
Sbjct: 18 SFEYFPPKTTVGLENLSSRITRMSRNMMPLFSSVTWGTAGSTAEVSIYLAKMLEQHHKIP 77
Query: 86 VCMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEWSPDADTEFKYAVDLVRY 145
C+HLTCTN++K IID+AL+ A++ IRNILALRGDP + + +EF++AVDLVRY
Sbjct: 78 ACLHLTCTNVDKAIIDKALETAKEYGIRNILALRGDPPLNSDHWEGKVSEFEHAVDLVRY 137
Query: 146 IKKNYGDDFCVGVAAYPEGHCEGEADESLQDPMRDLPYLKEKVDAGADFIITQLFYDVDK 205
I++ YGD FC+GVAAYPEGH + E +DP+RD+P+L EKV+AGADFIITQ+FY+ +
Sbjct: 138 IREKYGDYFCIGVAAYPEGHVDSNVPELSKDPLRDIPFLIEKVEAGADFIITQIFYEPEA 197
Query: 206 FLSFEKLVREKVSS---DIPMFPGLMPINSHLLFNRAAKLSHASIPQYILDRFPASVQSD 262
F+ FE VR S+ +IP+ P +MPI S+ R +L S+P ++ R A+ + D
Sbjct: 198 FIKFENFVRNHSSNALRNIPIIPAIMPIQSYGSLKRMTRLCGCSVPSSLMQRLNAA-KPD 256
Query: 263 DNRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIVAKSPTLSKI---LXX 319
D +K GR+ FHF TLNLER++A I+ S L+K +
Sbjct: 257 DEAIKNIGVEHIVDMIKKIMDNVQGRVHGFHFCTLNLERSVALILKNSGLLTKRWKQVES 316
Query: 320 XXXXXXXXXXXXXXXXXXXXXXXXXNIKKRKRQSSASTDFPLNTSIINKLNGMS-HGNRV 378
+ +Q A D N + +K + +S H + +
Sbjct: 317 EMEDEKLLRTTRKRLSLDEPAELHNQVVVPSQQPVA--DKSSNLFVTSKQSSVSGHKDNL 374
Query: 379 PSVPSRKVMISISQGTGVLGRNVTWDEFPNGRFGDSRSPAYGEIDGYGPSLKVCNSTAYE 438
+ + S+S+G+GVLGR WD+F NGRFGD RSPAYGEIDGYGP+L S A +
Sbjct: 375 ----TEEAPFSVSEGSGVLGRQANWDDFTNGRFGDPRSPAYGEIDGYGPTLHFPPSEALK 430
Query: 439 LWDHPVSLEDIKNIFIRYLEGSMKALPWSDLGLSAETAFIQEELIELNQRGYLSLASQPA 498
LW +PV DI ++F +++ + A+PW D + ET I + L++LN + ++ SQPA
Sbjct: 431 LWGYPVDESDITSLFQKHIMSDISAIPWIDEPVEVETKTIAKYLLKLNGNSWWTVGSQPA 490
Query: 499 TNGSQSTDNIFGWGPANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEFSYYLGDYTGY 558
NG+ S D +FGWGP G ++QKAFVE FV ++ + + N + +YY G+
Sbjct: 491 VNGAPSADPVFGWGPKGGRVFQKAFVECFVNGKDLKDFITKWHD--NPQVTYYAGNNKSE 548
Query: 559 FETNLQPKSSNAVTWGVFPNSEVVQTTIVEEESFKAWRDEAFSIWIEWSKLFPKNTPENT 618
F TN ++AVTWGV+P E++Q+TI+ E SFKAW E+F +W EW+ L+ KNTP
Sbjct: 549 FLTNAPKDGASAVTWGVYPGREIIQSTIIAEVSFKAWLSESFQVWGEWANLYSKNTPSRK 608
Query: 619 FLRKMHRDYYLVSIVHHDFINPDGLWDLL 647
L D +LV+++HHDF++ +GLW +L
Sbjct: 609 LLENCINDRWLVTVIHHDFMDKNGLWKVL 637
>SPAC56F8.10 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 603
Score = 397 bits (1021), Expect = e-110
Identities = 228/622 (36%), Positives = 336/622 (53%), Gaps = 59/622 (9%)
Query: 27 SLEFFPPKTEPGKRNLLARMERMCALN-PLFIXXXXXXXXXXXXXXXXXXXIAQRELNVP 85
S EFFPPKT G +NL R++RM P+F+ + Q + V
Sbjct: 19 SYEFFPPKTSTGVQNLYNRIDRMKTWGRPMFVDVTWGAGGTSSELTPGIVNVIQTDFEVD 78
Query: 86 VCMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEWSPDADTEFKYAVDLVRY 145
CMHLTCTNM+ ++ID ALK A + RNILALRGDP +W+ + ++ F+YA DLVRY
Sbjct: 79 TCMHLTCTNMSTEMIDAALKRAHETGCRNILALRGDPVKDTDWT-EGESGFRYASDLVRY 137
Query: 146 IKKNYGDDFCVGVAAYPEGHC-EGEADESLQDPMRDLPYLKEKVDAGADFIITQLFYDVD 204
I+ +Y D+FC+GVA YPEG+ + + DES++ +LK KVD GADFI+TQ+FYDVD
Sbjct: 138 IRTHYNDEFCIGVAGYPEGYSPDDDIDESIK-------HLKLKVDEGADFIVTQMFYDVD 190
Query: 205 KFLSFEKLVREKVSSDIPMFPGLMPINSHLLFNRAAKLSHASIPQYILDRFPASVQSDDN 264
F+++ VR +IP+FPG+MPI + F R AK S IPQ+ +D V+ DD
Sbjct: 191 NFIAWVDKVRA-AGINIPIFPGIMPIQAWDSFIRRAKWSGVKIPQHFMDTL-VPVKDDDE 248
Query: 265 RVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIVAKSPTLSKILXXXXXXX 324
V+ R+ HFYT+NLE+A+ I+ + L + L
Sbjct: 249 GVRERGVELIVEMCRKLIASGITRL---HFYTMNLEKAVKMIIERLGLLDENLAPI---- 301
Query: 325 XXXXXXXXXXXXXXXXXXXXNIKKRKRQSSASTDFPLNTSIINKLNGMSHGNRVPSVPSR 384
++T+ + N S R+
Sbjct: 302 ------------------------------------VDTNNVELTNASSQDRRINE--GV 323
Query: 385 KVMISISQGTGVLGRNVTWDEFPNGRFGDSRSPAYGEIDGYGPSLKVCNSTAYELWDHPV 444
+ + ++ + R WDE P+GR+GDSRSPA+GE D L++ W P
Sbjct: 324 RPIFWRTRNESYVSRTDQWDELPHGRWGDSRSPAFGEFDAIRYGLRMSPKEITTSWGSPK 383
Query: 445 SLEDIKNIFIRYLEGSMKALPWSDLGLSAETAFIQEELIELNQRGYLSLASQPATNGSQS 504
S +I ++F RY E + +LPWSDL +S E I+++L+ +N+ +L++ SQPA NG +S
Sbjct: 384 SYSEIGDLFARYCEKKISSLPWSDLPISDEADLIRDQLLSMNRNAFLTINSQPALNGEKS 443
Query: 505 TDNIFGWGPANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEFSYYLGDYTGYFETNLQ 564
+ +FGWGP NG ++QK +VE FV N+ LK ++ N SY++ + G +TN Q
Sbjct: 444 SHPVFGWGPPNGYVFQKPYVEFFVHPSLLNE-LKETVKKLN-SVSYFVTNKNGDLDTNSQ 501
Query: 565 PKSSNAVTWGVFPNSEVVQTTIVEEESFKAWRDEAFSIWIEWSKLFPKNTPENTFLRKMH 624
+ NAVTWGVFPN E++Q TIVE SF AW+DEA+S+ +EW+ + ++ L M
Sbjct: 502 YEIPNAVTWGVFPNREIIQPTIVESTSFLAWKDEAYSLGMEWANAYSPDSISRKLLVSMM 561
Query: 625 RDYYLVSIVHHDFINPDGLWDL 646
++++L IV +DF N L+D+
Sbjct: 562 KEWFLCVIVDNDFQNGQSLFDV 583
>Hs6631098 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 656
Score = 198 bits (503), Expect = 2e-50
Identities = 101/261 (38%), Positives = 158/261 (59%), Gaps = 13/261 (4%)
Query: 399 RNVTWDEFPNGRFGDSRSPAYGEIDGYGPSLKVCNSTAYEL---WDHPVSLE-DIKNIFI 454
R WDEFPNGR+G+S SPA+GE+ Y S EL W ++ E + +F+
Sbjct: 377 RTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEASVFEVFV 436
Query: 455 RYLEGS-------MKALPWSDLGLSAETAFIQEELIELNQRGYLSLASQPATNGSQSTDN 507
YL G + LPW+D L+AET+ ++EEL+ +N++G L++ SQP NG S+D
Sbjct: 437 LYLSGEPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDP 496
Query: 508 IFGWGPANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEFSYYLGDYTGYFETNLQPKS 567
I GWGP+ G ++QKA++E F +E +L+ ++ +Y+L + G TN
Sbjct: 497 IVGWGPSGGYVFQKAYLEFFTSRETAEALLQ-VLKKYELRVNYHLVNVKGENITNAPELQ 555
Query: 568 SNAVTWGVFPNSEVVQTTIVEEESFKAWRDEAFSIWIE-WSKLFPKNTPENTFLRKMHRD 626
NAVTWG+FP E++Q T+V+ SF W+DEAF++WIE W KL+ + +P T ++ +H +
Sbjct: 556 PNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDN 615
Query: 627 YYLVSIVHHDFINPDGLWDLL 647
Y+LV++V +DF + LW ++
Sbjct: 616 YFLVNLVDNDFPLDNCLWQVV 636
Score = 161 bits (407), Expect = 3e-39
Identities = 102/287 (35%), Positives = 144/287 (49%), Gaps = 16/287 (5%)
Query: 27 SLEFFPPKTEPGKRNLLARMERMCALNPLFIXXXXXXXXX-XXXXXXXXXXIAQRELN-- 83
SLEFFPP+T G NL++R +RM A PL+I IA +N
Sbjct: 61 SLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSMMIASTAVNYC 120
Query: 84 -VPVCMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEWSPDADTEFKYAVDL 142
+ +H+TC + I L A+ ++NI+ALRGDP IG++W + + F YAVDL
Sbjct: 121 GLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDP-IGDQWEEE-EGGFNYAVDL 178
Query: 143 VRYIKKNYGDDFCVGVAAYPEGHCEGEADESLQDPMRDLPYLKEKVDAGADFIITQLFYD 202
V++I+ +GD F + VA YP+GH E + E+ DL +LKEKV AGADFIITQLF++
Sbjct: 179 VKHIRSEFGDYFDICVAGYPKGHPEAGSFEA------DLKHLKEKVSAGADFIITQLFFE 232
Query: 203 VDKFLSFEKLVREKVSSDIPMFPGLMPINSHLLFNRAAKLSHASIPQYILDRFPASVQSD 262
D F F K + + P+ PG+ PI + + KLS +PQ I D
Sbjct: 233 ADTFFRFVKACTD-MGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKD---VIEPIK 288
Query: 263 DNRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIVAK 309
DN +G + HFYTLN E A +++ +
Sbjct: 289 DNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKR 335
>YGL125w [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 599
Score = 193 bits (491), Expect = 6e-49
Identities = 109/261 (41%), Positives = 158/261 (59%), Gaps = 11/261 (4%)
Query: 397 LGRNVTW--DEFPNGRFGDSRSPAYGEIDGYGPSL-KVCNSTAYELWDHPVSLEDIKNIF 453
+ R W DEFPNGRFGDS SPA+G++D G L + + ELW P S+ D+ +
Sbjct: 341 VARTSQWAVDEFPNGRFGDSSSPAFGDLDLCGSDLIRQSANKCLELWSTPTSINDVAFLV 400
Query: 454 IRYLEGSMKALPWSDLGLSAETAFIQEELIELNQRGYLSLASQPATNGSQSTDNIFGWGP 513
I YL G++K LPWSD+ ++ E I+ LIELNQ +++ SQP NG +S D I GWGP
Sbjct: 401 INYLNGNLKCLPWSDIPINDEINPIKAHLIELNQHSIITINSQPQVNGIRSNDKIHGWGP 460
Query: 514 ANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEF-SYYLGDYTGYFETNLQPKS-SNAV 571
+G +YQK ++E + K + +++ KN EF +Y+ D G +N S SNAV
Sbjct: 461 KDGYVYQKQYLEFMLPKTKLPKLID---TLKNNEFLTYFAIDSQGDLLSNHPDNSKSNAV 517
Query: 572 TWGVFPNSEVVQTTIVEEESFKAWRDEAFSIWIEWSKLFPKNTPENT--FLRKMHRDYYL 629
TWG+FP E++Q TIVE+ SF AW++E + I EW K ++ F++ + DY L
Sbjct: 518 TWGIFPGREILQPTIVEKISFLAWKEEFYHILNEWKLNMNKYDKPHSAQFIQSLIDDYCL 577
Query: 630 VSIVHHDFINPDG-LWDLLLS 649
V+IV +D+I+PD + +LLS
Sbjct: 578 VNIVDNDYISPDDQIHSILLS 598
Score = 173 bits (438), Expect = 8e-43
Identities = 112/289 (38%), Positives = 149/289 (50%), Gaps = 13/289 (4%)
Query: 24 PCVSLEFFPPKTEPGKRNLLARMERMCALN-PLFIXXX-XXXXXXXXXXXXXXXXIAQRE 81
P S E+F PKT G +NL RM+RM + P FI AQ
Sbjct: 17 PTYSFEYFVPKTTQGVQNLYDRMDRMYEASLPQFIDITWNAGGGRLSHLSTDLVATAQSV 76
Query: 82 LNVPVCMHLTCTNMNKDIIDEALKAARDADIRNILALRGD-PAIGEEWSPDADTEFKYAV 140
L + CMHLTCTNM +ID+AL+ A + +NILALRGD P E W+P + F+YA
Sbjct: 77 LGLETCMHLTCTNMPISMIDDALENAYHSGCQNILALRGDPPRDAENWTP-VEGGFQYAK 135
Query: 141 DLVRYIKKNYGDDFCVGVAAYPEGHCEGEADESLQDPMRDLPYLKEKVDAGADFIITQLF 200
DL++YIK YGD F +GVA YPE H E +D DL YL + G DFIITQ+F
Sbjct: 136 DLIKYIKSKYGDHFAIGVAGYPECHPE----LPNKDVKLDLEYLSRR-STGGDFIITQMF 190
Query: 201 YDVDKFLSFEKLVREKVSSDIPMFPGLMPINSHLLFNRAAKLSHASIPQYILDRFPASVQ 260
YDVD L++ VR D+P+ PG+MPI ++ F R + SIPQ+ R ++
Sbjct: 191 YDVDNLLNWCSQVR-AAGMDVPIIPGIMPITTYAAFLRRIQWGQISIPQHFSSRLD-PIK 248
Query: 261 SDDNRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIVAK 309
DD V+ +G + H YT+NLE+A I+ +
Sbjct: 249 DDDELVRDIGTNLIVEMCQKLL--DSGYVSHLHIYTMNLEKAPLMILER 295
>At2g44160 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 606
Score = 179 bits (453), Expect = 2e-44
Identities = 96/249 (38%), Positives = 147/249 (58%), Gaps = 6/249 (2%)
Query: 397 LGRNVTWDEFPNGRFGDSRSPAYGEIDGYGPSL-KVCNSTAYELWDHPV-SLEDIKNIFI 454
+ R W++FP GR+GDSRS +YG + + S + + + W P+ S+EDI+ F
Sbjct: 352 ISRTKGWEDFPQGRWGDSRSASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFK 411
Query: 455 RYLEGSMKALPWSDL-GLSAETAFIQEELIELNQRGYLSLASQPATNGSQSTDNIFGWGP 513
G++K+ PWS+L GL ET I E+LI++N +G+L++ SQP+ N +S GWG
Sbjct: 412 ELCLGNLKSSPWSELDGLQPETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGG 471
Query: 514 ANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEFSYYLGDYTGYFETNLQPKSSNAVTW 573
G +YQKA++E F KE+ + V++ K ++ +Y + + +N NAVTW
Sbjct: 472 PVGYVYQKAYLEFFCSKEKLDAVVE-KCKAL-PSITYMAVNKGEQWVSNTAQADVNAVTW 529
Query: 574 GVFPNSEVVQTTIVEEESFKAWRDEAFSIWI-EWSKLFPKNTPENTFLRKMHRDYYLVSI 632
GVFP E++Q TIV+ SF W+DEAF W W+ L+P+ P L ++ YYLVS+
Sbjct: 530 GVFPAKEIIQPTIVDPASFNVWKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSL 589
Query: 633 VHHDFINPD 641
V +D+IN D
Sbjct: 590 VENDYINGD 598
Score = 164 bits (414), Expect = 5e-40
Identities = 107/296 (36%), Positives = 141/296 (47%), Gaps = 20/296 (6%)
Query: 27 SLEFFPPKTEPGKRNLLARMERMCALNPLFIXXXXXXXXXXXXXXXXXXXIAQRELNVPV 86
S EFFPPKTE G NL RM+RM A P F Q + V
Sbjct: 19 SFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGSTADLTLDIASRMQNVVCVES 78
Query: 87 CMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEWSPDADTEFKYAVDLVRYI 146
MHLTCTNM + ID AL+ R I+N+LALRGDP G++ + F A+DLV +I
Sbjct: 79 MMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQDKFVQVEGGFDCALDLVNHI 138
Query: 147 KKNYGDDFCVGVAAYP------------EGHCEGEADESL---QDPMRDLPYLKEKVDAG 191
+ YGD F + VA YP E H + + L + DL YLK+K+DAG
Sbjct: 139 RSKYGDYFGITVAGYPAKDWIFMCFDISEAHPDVIGENGLASNEAYQSDLEYLKKKIDAG 198
Query: 192 ADFIITQLFYDVDKFLSFEKLVREKVSSDIPMFPGLMPINSHLLFNRAAKLSHASIPQYI 251
AD I+TQLFYD D FL F R+ + P+ PG+MPIN++ F R IP +
Sbjct: 199 ADLIVTQLFYDTDIFLKFVNDCRQ-IGISCPIVPGIMPINNYRGFLRMTGFCKTKIPVEV 257
Query: 252 LDRFPASVQSDDNRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIV 307
+ A DN +G +K+ H YTLN+E++ I+
Sbjct: 258 M---AALEPIKDNEEAVKAYGIHLGTEMCKKMLAHG-VKSLHLYTLNMEKSALAIL 309
>At3g59970 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 592
Score = 177 bits (448), Expect = 6e-44
Identities = 93/249 (37%), Positives = 146/249 (58%), Gaps = 6/249 (2%)
Query: 397 LGRNVTWDEFPNGRFGDSRSPAYGEIDGYGPSL-KVCNSTAYELWDHPV-SLEDIKNIFI 454
+ R W++FP+GR+GDS S AY + Y + K + + W P+ S+ED++ F
Sbjct: 340 ISRTKGWNDFPHGRWGDSHSAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFK 399
Query: 455 RYLEGSMKALPWSDL-GLSAETAFIQEELIELNQRGYLSLASQPATNGSQSTDNIFGWGP 513
G++K+ PWS+L GL ET I E+L ++N G+L++ SQP+ N ++S GWG
Sbjct: 400 ELCIGNLKSSPWSELDGLQPETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGG 459
Query: 514 ANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEFSYYLGDYTGYFETNLQPKSSNAVTW 573
G +YQKA++E F K++ + +++ +Y + + + +N NAVTW
Sbjct: 460 PGGYVYQKAYLEFFCSKDKLDTLVEK--SKAFPSITYMAVNKSENWVSNTGESDVNAVTW 517
Query: 574 GVFPNSEVVQTTIVEEESFKAWRDEAFSIWI-EWSKLFPKNTPENTFLRKMHRDYYLVSI 632
GVFP EV+Q TIV+ SFK W+DEAF IW W+ L+P++ P L ++ YYLVS+
Sbjct: 518 GVFPAKEVIQPTIVDPASFKVWKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSL 577
Query: 633 VHHDFINPD 641
V +++IN D
Sbjct: 578 VDNNYINGD 586
Score = 171 bits (432), Expect = 4e-42
Identities = 105/284 (36%), Positives = 142/284 (49%), Gaps = 8/284 (2%)
Query: 27 SLEFFPPKTEPGKRNLLARMERMCALNPLFIXXXXXXXXXXXXXXXXXXXIAQRELNVPV 86
S EFFPPKTE G NL RM+R+ + P F Q + V
Sbjct: 19 SFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGSTADLTLEIASRMQNVICVET 78
Query: 87 CMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEWSPDADTEFKYAVDLVRYI 146
MHLTCTNM + ID AL+ R I+N+LALRGDP G++ + F A+DLV +I
Sbjct: 79 MMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQDKFVQVEGGFACALDLVNHI 138
Query: 147 KKNYGDDFCVGVAAYPEGHCEGEADESLQDP---MRDLPYLKEKVDAGADFIITQLFYDV 203
+ YGD F + VA YPE H + + L P DL YLK+KVDAGAD I+TQLFYD
Sbjct: 139 RSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAYLKKKVDAGADLIVTQLFYDT 198
Query: 204 DKFLSFEKLVREKVSSDIPMFPGLMPINSHLLFNRAAKLSHASIPQYILDRFPASVQSDD 263
D FL F R+ + + P+ PG+MPI+++ F R A IP + ++ +D
Sbjct: 199 DIFLKFVNDCRQ-IGINCPIVPGIMPISNYKGFLRMAGFCKTKIPAELTAAL-EPIKDND 256
Query: 264 NRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIV 307
VK I + H YTLN++++ I+
Sbjct: 257 EAVKAYGIHFATEMCKKILAH---GITSLHLYTLNVDKSAIGIL 297
>CE02451 [E] KOG0564 510-methylenetetrahydrofolate reductase
Length = 615
Score = 151 bits (381), Expect = 3e-36
Identities = 78/214 (36%), Positives = 128/214 (59%), Gaps = 11/214 (5%)
Query: 445 SLEDIKNIFIRYLEGSMKA-------LPWSDL--GLSAETAFIQEELIELNQRGYLSLAS 495
S ED+K +FI Y+ + A LPW++ G+ ET+ I E+L+ N+ G L++ S
Sbjct: 393 SFEDVKRVFINYITQAPNADGVKVTVLPWTEAETGVQPETSLISEQLVWCNENGILTVNS 452
Query: 496 QPATNGSQSTDNIFGWGPANGLIYQKAFVEIFVRKEEWNQVLKPKIESKNGEFSYYLGDY 555
QP+ NG+ STD + GWG G YQKA++E F+ E +++++ +Y+ +
Sbjct: 453 QPSVNGAPSTDPLVGWGKPGGYCYQKAYLECFMTAELSDKLIQIIEREFPVRVNYHAINK 512
Query: 556 TGYFE-TNLQPKSSNAVTWGVFPNSEVVQTTIVEEESFKAWRDEAFSIWI-EWSKLFPKN 613
F+ TN + + AVTWGVFP SE+ Q T+V+ SF+AWRDEA+ +W+ +W +PK
Sbjct: 513 DSTFDKTNSEETTPIAVTWGVFPGSEIAQPTVVDPLSFRAWRDEAYQMWMAQWGDFYPKE 572
Query: 614 TPENTFLRKMHRDYYLVSIVHHDFINPDGLWDLL 647
+ ++ +H ++ LV++V +DF P L+D+L
Sbjct: 573 SKSYGVIKAVHDEFRLVTLVDNDFQKPSVLFDVL 606
Score = 144 bits (364), Expect = 3e-34
Identities = 102/299 (34%), Positives = 151/299 (50%), Gaps = 22/299 (7%)
Query: 16 QRFDELKGPCVSLEFFPPKTEPGKRNLLARMERMCALNPLFIXXXXXXXX--XXXXXXXX 73
+R + K SLEFFPP+ G N L R+ER+ +F+
Sbjct: 63 ERLIDEKQQFFSLEFFPPRFVNGVPNFLERVERLSEGGSVFVDMTWHMGSDPANVDKVTS 122
Query: 74 XXXIAQRELN---VPVCMHLTCTNMNKDIIDEALKAARDADIRNILALRGDPAIGEEWSP 130
IA L+ V +H+TC NK + L+ A+ +R+ILALRGD G E
Sbjct: 123 SSSIAASMLDYCGVDTMLHMTCVQYNKADTLKHLEQAKAMGLRSILALRGDLPPGTELE- 181
Query: 131 DADTEFKYAVDLVRYIKKNYGDDFCVGVAAYPEGHCEGEADESLQDPMRDLPYLKEKVDA 190
DT A+D++R+I++ YG+ F +G A YP GH + + ++ DL YLK K DA
Sbjct: 182 --DTHQFRALDMIRWIREEYGNYFSIGCAGYPLGHPQAPSYKA------DLMYLKAKCDA 233
Query: 191 GADFIITQLFYDVDKFLSFEKLVRE--KVSSDIPMFPGLMPINSHLLFNRAAKLSHASIP 248
GA+F+ITQLF++ + +FEK VR+ ++ P+ PG+MPI + R AKLS IP
Sbjct: 234 GANFVITQLFFEAE---TFEKFVRDCREIGITQPIIPGIMPIMGYESIKRIAKLSQLEIP 290
Query: 249 QYILDRFPASVQSDDNRVKXXXXXXXXXXXXXXXXKTNGRIKAFHFYTLNLERAIAQIV 307
Q+ILD ++ DD+ V+ NG + H YT+N E +I +I+
Sbjct: 291 QHILDDL-EPIKHDDDAVQKYGTERCIEMCRRLL--DNGTAPSIHLYTMNREGSIREIL 346
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,402,079
Number of Sequences: 60738
Number of extensions: 1583855
Number of successful extensions: 3417
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3348
Number of HSP's gapped (non-prelim): 17
length of query: 649
length of database: 30,389,216
effective HSP length: 113
effective length of query: 536
effective length of database: 23,525,822
effective search space: 12609840592
effective search space used: 12609840592
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)