ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3246 good S KOG2632 Function unknown Rhomboid family proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV3246 1094184 1093372 -271 
         (271 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YPL246c [S] KOG2632 Rhomboid family proteins                          254   7e-68
SPCC790.03 [S] KOG2632 Rhomboid family proteins                        72   6e-13
At3g59520 [S] KOG2632 Rhomboid family proteins                         49   6e-06

>YPL246c [S] KOG2632 Rhomboid family proteins
          Length = 262

 Score =  254 bits (650), Expect = 7e-68
 Identities = 124/257 (48%), Positives = 178/257 (69%), Gaps = 3/257 (1%)

Query: 1   MNIKQFFVPAGKPLSGLAVGLSIFLTALFLVNNLVYPINEHLLLKPDSLFKFDLNRISLY 60
           MN K +  P G P + L  GL +FLTA++L++  ++ + E L L P+SLFK  ++R+SLY
Sbjct: 1   MNWKSYVFPGGHPPAALTTGLVVFLTAIYLLS-FIFALREDLSLAPESLFKLQMSRLSLY 59

Query: 61  PLAHLSFFHLFFNVISTFSMIVMFEESHGTLYTGVILNLLAVFTAIPYCLIGSLLFPNVE 120
           PL HLS  HL FNV++ ++ + +FEE+HGT+YTGV LNL A+F  I YCL+G LL+P   
Sbjct: 60  PLIHLSLPHLLFNVLAIWAPLNLFEETHGTVYTGVFLNLSALFAGILYCLLGKLLYPEAL 119

Query: 121 IGGASGWFFSFLGYFAVKESRVRNSVMITST-FSFPTLYFPVALLFVTALLAPGSSLPGH 179
           + GASGW F+   Y++ KES++R    I  T +S PTLY P+ LL   A++ PGSS  GH
Sbjct: 120 VAGASGWCFTLFAYYSFKESQIRPRTRIFRTDYSIPTLYTPLVLLVAIAVVIPGSSFWGH 179

Query: 180 AIGLLLGYFMGLKENWVAKITPPSFVLKKIETWVDPLINLIPFGIKYYREVEVDRSLEYT 239
             GL +GY +G KE+W  KITPP +++ KIE  +D LI LIP+GIKYYR+ ++DR+ +Y 
Sbjct: 180 FFGLCVGYAIGYKESWFNKITPPGWIITKIEKSLDGLIRLIPWGIKYYRDEDIDRTKDYE 239

Query: 240 SVYLGSESRLPLHNTDT 256
            + + +E+ LPLHN ++
Sbjct: 240 PL-MSTETPLPLHNDNS 255


>SPCC790.03 [S] KOG2632 Rhomboid family proteins
          Length = 248

 Score = 72.4 bits (176), Expect = 6e-13
 Identities = 47/149 (31%), Positives = 66/149 (43%), Gaps = 19/149 (12%)

Query: 49  LFKFDLNRISLYPLAHLSFFHLFFNVISTFSMIVMFEESHGTLYTGVILNLLAVFTAIPY 108
           L K  L  I  Y   HLS  H+ FN +S    +  FE+  GTL   ++       T IPY
Sbjct: 48  LQKRQLYEIITYVTLHLSMLHIVFNFVSLLPAMSQFEKKQGTLACILV-------TVIPY 100

Query: 109 CLIGSLLF----------PNVEIGGASGWFFSFLGYFAVKESRVRNSVMITSTFSFPTLY 158
            L   ++             V I G SGW F+F+    V   +    +   + FS P   
Sbjct: 101 TLFPGIMHLIVYHFFLRKDYVSIAGLSGWAFAFISASCVHSPQ--RLISFFNLFSIPAYC 158

Query: 159 FPVALLFVTALLAPGSSLPGHAIGLLLGY 187
           FP+  L +T +L P +S  GHA G ++GY
Sbjct: 159 FPIIYLIMTTILVPKASFIGHASGAVMGY 187


>At3g59520 [S] KOG2632 Rhomboid family proteins
          Length = 269

 Score = 49.3 bits (116), Expect = 6e-06
 Identities = 39/137 (28%), Positives = 65/137 (46%), Gaps = 13/137 (9%)

Query: 62  LAHLSFFHLFFNVISTFSMIVMFEESHGTLYTGVILN---LLAVFTAIPYCLIGSLL--- 115
           L+H+S  HL FN+ + +S+ V+ +  H  L T   L+   +L VF+ +    I  LL   
Sbjct: 59  LSHISVLHLVFNMSALWSLGVVEQLGHVGLGTAYYLHYTLVLVVFSGVLVIGIYHLLIAR 118

Query: 116 -----FPNVEIGGASGWFFSFLGYFAVKESRVRNSVMITSTFSFPTLYFPVALLFVTALL 170
                F  V   G S   F ++   +VK+    + + +    S P  + P   L  T+++
Sbjct: 119 FKIDYFRRVTAVGYSCVVFGWMTILSVKQPS--SKLNLFGLLSLPISFAPFESLIFTSII 176

Query: 171 APGSSLPGHAIGLLLGY 187
            P +S  GH  G+L+GY
Sbjct: 177 VPQASFLGHLSGILVGY 193


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.326    0.144    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,404,713
Number of Sequences: 60738
Number of extensions: 684018
Number of successful extensions: 1828
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1821
Number of HSP's gapped (non-prelim): 4
length of query: 271
length of database: 30,389,216
effective HSP length: 105
effective length of query: 166
effective length of database: 24,011,726
effective search space: 3985946516
effective search space used: 3985946516
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)