ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3260 good U KOG2460 Intracellular trafficking, secretion, and vesicular transport Signal recognition particle, subunit Srp68
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3260 1097784 1099535 584
(584 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL243w [U] KOG2460 Signal recognition particle subunit Srp68 382 e-105
Hs7657617 [U] KOG2460 Signal recognition particle subunit Srp68 72 2e-12
Hs22062290 [U] KOG2460 Signal recognition particle subunit Srp68 71 4e-12
SPCC1682.05c [U] KOG2460 Signal recognition particle subunit Srp68 57 8e-08
>YPL243w [U] KOG2460 Signal recognition particle subunit Srp68
Length = 599
Score = 382 bits (980), Expect = e-105
Identities = 218/600 (36%), Positives = 346/600 (57%), Gaps = 22/600 (3%)
Query: 1 MVYSPLGVTYGFRSNLLLESFADYHKYHKKVNHKLQQLRGRLKLISKDSKNYKGQEKLDK 60
+ YSP+ TYG R+ LE+ +D+ KYH K+N KLQ LR R L++KD+K Y + K +
Sbjct: 2 VAYSPIIATYGNRAEQFLETDSDFAKYHAKLNKKLQHLRSRCHLVTKDTKKYSSKNKYGE 61
Query: 61 ITHEDY-SKNRLYGFLMLLLAERDVAYVESIKATGRSRGHLRSLELKLMRTRIKKANRYC 119
I EDY +K +L G L+LL AERD+A E++K R RG L+ E K++ TR+KKA +
Sbjct: 62 INSEDYDNKTKLIGVLILLHAERDLALAETLKLRARQRGKLKKSEEKVLSTRLKKACKTA 121
Query: 120 DRLISLSQGEDKWQIQAEIMIYEKLIRTEYELYGKKQVKFSDAEKISTHXXXXXXXVNFL 179
D+L++++Q E +W +A+ + + KL+ +EY + GK+ K D KIS + + L
Sbjct: 122 DKLVNVTQNEQQWITRAQYLAFAKLVHSEYLINGKR-FKRKDNAKISNNLALVFAALEHL 180
Query: 180 VDSKLLKEGVADAVKSKYLYIFKEKA-NAFSVRDQQTYISRVIQA--NPEDQLLQLLQSH 236
+ LL E V D + +KY Y K+ A N + + +I +Q+ N +D+L++LL +
Sbjct: 181 KNLSLLAEEVVDNIVNKYQYSLKQYAGNLITTPEINNFIVERVQSDENKDDELVKLLLDN 240
Query: 237 GFSFSVPETDASKSQ-SREISWRSYSATIEDSKVFEVYSELAELHNSSKLSNLNDKLFKW 295
GF+ T + + I+WRS++A I D++V + + +H ++++ +L K
Sbjct: 241 GFNMKKITTSTEDQKVTTNINWRSFNAKIIDAEVAQFLEQGLSIH-PTQITQYTQRLSKL 299
Query: 296 NRAIDLQKEYMTTCSEDD--------TDENNQILLTYLQYNAMVTGVERDSSLFESLLKQ 347
+A+D + ++ + D + ENNQI+L Y++YN ++T + R+ LF L Q
Sbjct: 300 EKALDRHEFFIANHDDQDDIDEMVENSSENNQIILAYIKYNILLTSISRERDLFTHLWNQ 359
Query: 348 WERFDNKSFTSKLNKYKEVDHIIKNLKSLLSQIMDLPGVYSDSDLTNQVFLLKTYYETCF 407
W + N S SKL KYKE++ I+KNL LS IM+LPGVYSD +L +Q+ L K Y++
Sbjct: 360 WLKL-NTSLPSKLTKYKEMERIVKNLTKYLSDIMELPGVYSDDELLSQLDLCKLYFQLFL 418
Query: 408 TSKLLANIYQSKKKYVESLALYVDCLQRIENAEESLINWDEIYLPSQLLSKGKITDLKKY 467
+ L+ +YQSK +Y+E+LALYVD +R+EN + + DEI LP+ LLS + L+K
Sbjct: 419 NTGCLSVLYQSKGRYMEALALYVDAYRRLENKLSEIESLDEILLPANLLSLNSVRSLQKR 478
Query: 468 ITTGWRSVVALAEHKKSMEKNKKTRQFEPSLVEILES-KVRPSDVSLDNLYPLAPRLLPI 526
I G SV+ LAE++K +++ +++E L+S K+ P+D+ L NL+PL P++LPI
Sbjct: 479 IENGGNSVITLAEYEK-RNHGGSLGKYDLTVIEKLDSKKILPTDIQLKNLFPLKPKMLPI 537
Query: 527 PPKPFLYDLAYNYFNIPDXXXXXXXXXXXXXAEANQDDEPVK----ESKAPSKKGLFGLF 582
P KP L+DLA+NY + P+ E + K+G GLF
Sbjct: 538 PSKPTLFDLAFNYITYDKQEPSASQVKDSVTETESISQTPISNEQTEGEPKKKRGFLGLF 597
>Hs7657617 [U] KOG2460 Signal recognition particle subunit Srp68
Length = 619
Score = 72.0 bits (175), Expect = 2e-12
Identities = 122/559 (21%), Positives = 223/559 (39%), Gaps = 74/559 (13%)
Query: 23 DYHKYHKKVNHKLQQLRGRLKLISKDSKNYKGQEKLDKITHEDYSKNRLYGFLMLLLAER 82
D+ +Y + + ++LR L + + G+ K+T E + NR Y L+L+ AER
Sbjct: 71 DFQRYRGYCSRRQRRLRKTLNFKMGNRHKFTGK----KVTEELLTDNR-YLLLVLMDAER 125
Query: 83 DVAYVESIKATGRSRGHLRSLELKLMRTRIKKANRYCDRLISLSQGEDKWQIQAEIMIYE 142
+Y +K + R L +R +K A +RL ++ + K +++A+
Sbjct: 126 AWSYAMQLKQEANTEPRKRFHLLSRLRKAVKHAEEL-ERLCKSNRVDAKTKLEAQA---- 180
Query: 143 KLIRTEYELYGKKQVKFSDAEKISTHXXXXXXXVNFLVDSKLLKEGVADAVKSKYLYIFK 202
Y Y ++F E + K + E +A A + ++
Sbjct: 181 ------YTAYLSGMLRFEHQE--------WKAAIEAFNKCKTIYEKLASAFTEEQAVLYN 226
Query: 203 EKAN---------AFSVRDQQTYISRVIQANPEDQLLQLLQSHGFSFSVPETDASKSQSR 253
++ A+++ DQ I+ ++Q + L + + +T A ++ +
Sbjct: 227 QRVEEISPNIRYCAYNIGDQSA-INELMQMRLRSGGTEGLLAEKLEALITQTRAKQAATM 285
Query: 254 -EISWRSYSATIE------------DSKVFEVYSELAELHN---SSKLSNLNDKLFKWNR 297
E+ WR + ++ D++ V +E E S LS D +
Sbjct: 286 SEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDAIQVVRE 345
Query: 298 AI---DLQKEYMTTCSEDDTDENNQILLTYLQYNAMVTGVERDSSLFE----SLLKQWER 350
+ Q++Y+ E N Q L +YL Y + T ++R+ ++ + +LL+Q
Sbjct: 346 ELKPDQKQRDYILE-GEPGKVSNLQYLHSYLTYIKLSTAIKRNENMAKGLQRALLQQQPE 404
Query: 351 FDNKSFTSKLNKYKEVDHIIKNLKSLLSQIMDLPGVYSDSDLTNQVFLLKTYYETCFTSK 410
D+K + + D I++NL LL LPG+ D ++ L KT +
Sbjct: 405 DDSKRSPRPQDLIRLYDIILQNLVELLQ----LPGLEEDKAFQKEIGL-KTLVFKAYRCF 459
Query: 411 LLANIYQSKKKYVESLALYVDCLQRIENAEESLINWDEIYLPSQLLSKGKITDLKKYITT 470
+A Y KK+ E+L LY L+ +N D + L + +L + +
Sbjct: 460 FIAQSYVLVKKWSEALVLYDRVLKYANE-----VNSDAGAFKNSLKDLPDVQELITQVRS 514
Query: 471 GWRSVVALA--EHKKSMEKNKKTRQFEPS--LVEILESK-VRPSDVSLD-NLYPLAPRLL 524
S+ A A E + + + Q + + LVE E+ + PS V+ NL P
Sbjct: 515 EKCSLQAAAILESNDAHQTETSSSQVKDNKPLVERFETFCLDPSLVTKQANLVHFPPGFQ 574
Query: 525 PIPPKPFLYDLAYNYFNIP 543
PIP KP +DLA N+ P
Sbjct: 575 PIPCKPLFFDLALNHVAFP 593
>Hs22062290 [U] KOG2460 Signal recognition particle subunit Srp68
Length = 619
Score = 71.2 bits (173), Expect = 4e-12
Identities = 121/560 (21%), Positives = 224/560 (39%), Gaps = 76/560 (13%)
Query: 23 DYHKYHKKVNHKLQQLRGRLKLISKDSKNYKGQEKLDKITHEDYSKNRLYGFLMLLLAER 82
D+ +Y + + ++LR L + + G+ K+T E + NR Y L+L+ AER
Sbjct: 71 DFQRYRGYCSRRQRRLRKTLNFKMGNRHKFTGK----KVTEELLTDNR-YLLLVLMDAER 125
Query: 83 DVAYVESIKATGRSRGHLRSLELKLMRTRIKKANRYCDRLISLSQGEDKWQIQAEIMIYE 142
+Y +K + R L +R +K A +RL ++ + K +++A+
Sbjct: 126 AWSYAMQLKQEANTEPRKRFHLLSRLRKAVKHAEEL-ERLCESNRVDAKTKLEAQA---- 180
Query: 143 KLIRTEYELYGKKQVKFSDAEKISTHXXXXXXXVNFLVDSKLLKEGVADAVKSKYLYIFK 202
Y Y ++F E + K + E +A A + ++
Sbjct: 181 ------YTAYLSGMLRFEHQE--------WKAAIEAFNKCKTIYEKLASAFTEEQAVLYN 226
Query: 203 EKAN---------AFSVRDQQTYISRVIQANPEDQLLQLLQSHGFSFSVPETDASKSQSR 253
++ A+++ DQ I+ ++Q + L + + +T A ++ +
Sbjct: 227 QRVEEISPNIRYCAYNIGDQSA-INELMQMRLRSGGTEGLLAEKLEALITQTRAKQAATM 285
Query: 254 -EISWRSYSATIE------------DSKVFEVYSELAELHN---SSKLSNLNDKLFKWNR 297
E+ WR + ++ D++ V +E E S LS D +
Sbjct: 286 SEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDAIQVVRE 345
Query: 298 AI---DLQKEYMTTCSEDDTDENNQILLTYLQYNAMVTGVERDSSLFE----SLLKQWER 350
+ Q++Y+ E N Q L +YL Y + T ++R+ ++ + +LL+Q
Sbjct: 346 ELKPDQKQRDYILE-GEPGKVSNLQYLHSYLTYIKLSTAIKRNENMAKGLQRALLQQQPE 404
Query: 351 FDNKSFTSKLNKYKEVDHIIKNLKSLLSQIMDLPGVYSDSDLTNQVFLLKTYYETCFTSK 410
D+K + + D I++NL LL LPG+ D ++ L KT +
Sbjct: 405 DDSKRSPRPQDLIRLYDIILQNLVELLQ----LPGLEEDKAFQKEIGL-KTLVFKAYRCF 459
Query: 411 LLANIYQSKKKYVESLALYVDCLQRIENAEESLINWDEIYLPSQLLSKGKITDLKKYITT 470
+A Y KK+ E+L LY L+ +N D + L + +L + +
Sbjct: 460 FIAQSYVLVKKWSEALVLYDRVLKYANE-----VNSDAGAFKNSLKDLPDVQELITQVRS 514
Query: 471 GWRSVVALA-----EHKKSMEKNKKTRQFEPSLVEILESK-VRPSDVSLD-NLYPLAPRL 523
S+ A A + ++ + + + +P LVE E+ + PS V+ NL P
Sbjct: 515 EKCSLQAAAILDANDAHQTETSSSQVKDNKP-LVERFETFCLDPSLVTKQANLVHFPPGF 573
Query: 524 LPIPPKPFLYDLAYNYFNIP 543
PIP KP +DLA N+ P
Sbjct: 574 QPIPCKPLFFDLALNHVAFP 593
>SPCC1682.05c [U] KOG2460 Signal recognition particle subunit Srp68
Length = 597
Score = 57.0 bits (136), Expect = 8e-08
Identities = 117/551 (21%), Positives = 232/551 (41%), Gaps = 83/551 (15%)
Query: 16 LLLESFADY----HKYHKKVNHKLQQLRGRLKLISKDSKNYKGQEKLDKITHEDYSKNRL 71
LLLE+ +D+ ++Y K ++H++ LR L + + K +K
Sbjct: 64 LLLEARSDHFYEGNEYIKYLSHRIHGLRKSLHITQRGGKPKSSVDKR------------- 110
Query: 72 YGFLMLLLAERDVAYVESIKATGRSRGHLRSLELKLMRTRIKKANRYCDRLISLSQGEDK 131
+ +LL D A+ + + LRS + + R+K+A+++ L+S + D
Sbjct: 111 --YAEILLFNADRAFQQFV--------FLRSSQRRHALRRLKRADQFGKELVSFTNAFD- 159
Query: 132 WQIQAEIMIYEKLIRTEYELYGKKQVKFSDAEKISTHXXXXXXXVNFLVDSKLL------ 185
I E + +Y + G + D E + ++FLV +
Sbjct: 160 --CNDHIFYLEAIAFAKY-IEGTLNYEKRDWE--GSLSAFSISRLSFLVLQNKIDTLAEH 214
Query: 186 KEGVADAVKSKYLYIFKEKANAFSVRDQQTYISRVIQAN-PED----QLLQLLQSHGFSF 240
++ V ++++ + A +++Q + ++ ++ P+D Q + + S
Sbjct: 215 EKSVLGELQNQIDSNLRYVAQRTGLQNQTKSLDILMLSSIPKDEEVIQHVNSVDSEILQM 274
Query: 241 SVPETDASKSQSREISWRSYSATIEDSKVFEVYSELAELHNS-SKLSNLNDKLFK-WNRA 298
+ E D+ ++ + I WR IE V + ++ NS + + D+L W RA
Sbjct: 275 TGDEQDSLQTIT-VIEWRDQRVKIEHPDVVLALYAIHDVKNSPGTIDSKRDRLLAAWARA 333
Query: 299 IDLQKEYMTTCSEDDTD--ENNQILLTYLQYNAMVTGVERDSSLFESLLKQWERFDNKSF 356
++ K + +D + I TYL YN ++ ++RD ++ D++
Sbjct: 334 EEITKSVLDNTGLEDEQKFQTLSICYTYLAYNVVLLRIQRDLAV---------ENDSELV 384
Query: 357 TSKL---NKYKEVDHIIKNLKSLLSQIMDLPGVYSDSDLT----NQVFLLKTYYETCFTS 409
S+ ++ D IIKN++ +LPG+ D+ +T Q+ L K+ + C
Sbjct: 385 ASQAQLRSRQSLYDSIIKNIEI----AKELPGIARDTGMTAQLEAQISLAKS--KRC--- 435
Query: 410 KLLANIYQSKKKYVESLALYVDCLQRIENAEESLINWDEIYLPSQLLSKGKITDLKKYIT 469
+ +A+ YQ++ K + SLA+ V ++ + L N++E L++ I +LKK
Sbjct: 436 QAIADAYQAQDK-LASLAMCVRAASYLQQVNDILRNFEE---KPHLIAFDIIPELKKDEK 491
Query: 470 TGWRSVVALAEHKKSMEKNKKTRQFEPSLVEILESKVRPSDVSLDNLYPLAPR-LLPIPP 528
+ ++ L N+ + SLVE L+S ++ L+ + L + IP
Sbjct: 492 ELKKKILLLQSLASMGNINQPPKNL--SLVETLDSYQTLAE--LEPSWNLTDADIRAIPA 547
Query: 529 KPFLYDLAYNY 539
KP +DLA Y
Sbjct: 548 KPLFFDLAITY 558
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,280,664
Number of Sequences: 60738
Number of extensions: 1324733
Number of successful extensions: 4596
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4585
Number of HSP's gapped (non-prelim): 4
length of query: 584
length of database: 30,389,216
effective HSP length: 112
effective length of query: 472
effective length of database: 23,586,560
effective search space: 11132856320
effective search space used: 11132856320
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)