ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3297 good R KOG2564 General function prediction only Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3297 1116828 1115644 -395
(395 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHR075c [R] KOG2564 Predicted acetyltransferases and hydrolases ... 384 e-106
SPBP4H10.17c [R] KOG2564 Predicted acetyltransferases and hydrol... 188 1e-47
CE26705 [R] KOG2564 Predicted acetyltransferases and hydrolases ... 141 1e-33
Hs7706645 [R] KOG2564 Predicted acetyltransferases and hydrolase... 124 3e-28
7295008 [R] KOG2564 Predicted acetyltransferases and hydrolases ... 78 3e-14
>YHR075c [R] KOG2564 Predicted acetyltransferases and hydrolases with the
alpha/beta hydrolase fold
Length = 400
Score = 384 bits (985), Expect = e-106
Identities = 204/400 (51%), Positives = 267/400 (66%), Gaps = 13/400 (3%)
Query: 1 MSGNLQREALLKEFENAGKMIHA-FDTAPEGNGEE-DTITELPVV--------KNPGLGV 50
MS +L+R+ L +FE A ++ A F A E + + D + LP K G
Sbjct: 1 MSDDLRRKIALSQFERAKNVLDATFQEAYEDDENDGDALGSLPSFNGQSNRNRKYTGKTG 60
Query: 51 SSETKAENMEDTGFPRWTEFFAKNETVSLPNRNFTFNTYFQVPEVNSSDLSSIPIFLAHH 110
S+ + + E + P W++FF E VSLP+R+ NTY+ +P S+ +SIPIF+ HH
Sbjct: 61 STTDRISSKEKSSLPTWSDFFDNKELVSLPDRDLDVNTYYTLPTSLLSNTTSIPIFIFHH 120
Query: 111 GAGSTGLTFAPLAKTLKEDLGTNFGFFSFDARGHGETK-PLDPTDVTYYLNDFVTDFVEL 169
GAGS+GL+FA LAK L L G F+FDARGH ETK + + + F+ DFV L
Sbjct: 121 GAGSSGLSFANLAKELNTKLEGRCGCFAFDARGHAETKFKKADAPICFDRDSFIKDFVSL 180
Query: 170 IVWFYENYLKACKQSKLSLILVGHSLGGSVCANLYEHLPEYIKRHTTGLAMLDIVEEMAK 229
+ +++++ + K+S+IL+GHSLGGS+C Y L +++ G+ MLDIVEE A
Sbjct: 181 LNYWFKSKISQEPLQKVSVILIGHSLGGSICTFAYPKLSTELQKKILGITMLDIVEEAAI 240
Query: 230 FVLTKVDHFLSVTPNVFGSAKEAVDWYQSHNYSKLKESAEISVPALFHKAKSGKVVRITN 289
L KV+HFL TPNVF S +AVDW+ H S+L+ SAEI++PALF KSGKVVRITN
Sbjct: 241 MALNKVEHFLQNTPNVFESINDAVDWHVQHALSRLRSSAEIAIPALFAPLKSGKVVRITN 300
Query: 290 LASFKPYWDSWFDGLSAKFVSLPTSKLLILAGNDNLDKELIVGQMQGKYQLIVFQDSGHF 349
L +F P+WD+WF LS FV LP SKLLILAGN+NLDKELIVGQMQGKYQL+VFQDSGHF
Sbjct: 301 LKTFSPFWDTWFTDLSHSFVGLPVSKLLILAGNENLDKELIVGQMQGKYQLVVFQDSGHF 360
Query: 350 IQEDVPKKAAISLVDFWKRSDNRNVTIKTNWGSSQNKTLE 389
IQED P K AI+L+DFWKR+D+RNV IKTNWG Q+KT++
Sbjct: 361 IQEDSPIKTAITLIDFWKRNDSRNVVIKTNWG--QHKTVQ 398
>SPBP4H10.17c [R] KOG2564 Predicted acetyltransferases and hydrolases with the
alpha/beta hydrolase fold
Length = 341
Score = 188 bits (477), Expect = 1e-47
Identities = 115/324 (35%), Positives = 177/324 (54%), Gaps = 23/324 (7%)
Query: 51 SSETKAENME-DTGFPRWTEFFAKNETVSLPNR----NFTFNTYFQVPEVNSSDLSSIPI 105
SSETK + + W +F +E +++P N T N Y +P+ + +
Sbjct: 20 SSETKELMLNNEESLSDWRNYF--DEKLTIPGESGALNGTINGYLTLPQPDGC------L 71
Query: 106 FLAHHGAGSTGLTFAPLAKTLKEDLGTNFGFFSFDARGHGETKPLDPTDVTYYLNDFVTD 165
+ HGAGS+ ++FAP+ + L + GF + D R HGET +D++ L D
Sbjct: 72 LVLQHGAGSSAMSFAPVTQELLSNSDNKVGFLALDLRAHGETTLEPESDMS--LETLSKD 129
Query: 166 FVELIVWFYENYLKACKQSKLSLILVGHSLGGSVCANLYEHLPEYIKRHTTGLAMLDIVE 225
F + +Y++ + +ILVGHSLGG++CA Y + I +T+GL ++D+VE
Sbjct: 130 FTHAV-----SYVQRMFELDEKIILVGHSLGGAICA--YCAFQKTIP-NTSGLVVIDVVE 181
Query: 226 EMAKFVLTKVDHFLSVTPNVFGSAKEAVDWYQSHNYSKLKESAEISVPALFHKAKSGKVV 285
A L + +LS P F S +A+ W+ ++ + SA I+VP+L + + G V
Sbjct: 182 GTAMEALGFMKTYLSNRPTSFKSIDDAISWHIKTLVTRNRLSACITVPSLLVQQEDGTFV 241
Query: 286 RITNLASFKPYWDSWFDGLSAKFVSLPTSKLLILAGNDNLDKELIVGQMQGKYQLIVFQD 345
T+L PYW WF GLS KF+ P ++LI+AG D LDK L +GQMQGKYQL + +
Sbjct: 242 WRTDLYKTSPYWMDWFKGLSDKFLRAPYGRMLIVAGTDRLDKTLTIGQMQGKYQLEILPE 301
Query: 346 SGHFIQEDVPKKAAISLVDFWKRS 369
+GHF+ EDVP K + L++FW R+
Sbjct: 302 TGHFVHEDVPAKISSLLLNFWHRN 325
>CE26705 [R] KOG2564 Predicted acetyltransferases and hydrolases with the
alpha/beta hydrolase fold
Length = 364
Score = 141 bits (356), Expect = 1e-33
Identities = 96/311 (30%), Positives = 152/311 (48%), Gaps = 28/311 (9%)
Query: 51 SSETKAENMEDTGFPRWTEFFAKNETVSLPNRNFTFNTYFQVPEVNSSDLSSIPIFLAHH 110
S+ T + E + P W++FF + + ++ FN Y + E PIF H
Sbjct: 43 STSTSGKKREMSELP-WSDFFDEKKDANIDGD--VFNVYIKGNEG--------PIFYLLH 91
Query: 111 GAGSTGLTFAPLAKTLKEDLGTNFGFFSFDARGHGETKPLDPTDVTY--YLNDFVTDFVE 168
G G +GLT+A AK L + + D RGHG+TK D D++ + D F
Sbjct: 92 GGGYSGLTWACFAKELATLISCRV--VAPDLRGHGDTKCSDEHDLSKETQIKDIGAIFKN 149
Query: 169 LIVWFYENYLKACKQSKLSLILVGHSLGGSVCANLYEHLPEYIKRHTTGLAMLDIVEEMA 228
+ F E+ C +VGHS+GG++ + + I L ++D+VE A
Sbjct: 150 I---FGEDDSPVC--------IVGHSMGGALAIHTLN--AKMISSKVAALIVIDVVEGSA 196
Query: 229 KFVLTKVDHFLSVTPNVFGSAKEAVDWYQSHNYSKLKESAEISVPALFHKAKSGKVVRIT 288
L + HFL P+ F S ++A+ W S ++ +A +S+P+ + +
Sbjct: 197 MEALGGMVHFLHSRPSSFPSIEKAIHWCLSSGTARNPTAARVSMPSQIREVSEHEYTWRI 256
Query: 289 NLASFKPYWDSWFDGLSAKFVSLPTSKLLILAGNDNLDKELIVGQMQGKYQLIVFQDSGH 348
+L + + YW WF+GLS +F+ K+L+LAG D LD++L +GQMQGK+Q V GH
Sbjct: 257 DLTTTEQYWKGWFEGLSKEFLGCSVPKMLVLAGVDRLDRDLTIGQMQGKFQTCVLPKVGH 316
Query: 349 FIQEDVPKKAA 359
+QED P+ A
Sbjct: 317 CVQEDSPQNLA 327
>Hs7706645 [R] KOG2564 Predicted acetyltransferases and hydrolases with the
alpha/beta hydrolase fold
Length = 386
Score = 124 bits (310), Expect = 3e-28
Identities = 95/343 (27%), Positives = 146/343 (41%), Gaps = 66/343 (19%)
Query: 67 WTEFFAKNETVSLPNRNFTFNTYFQVPEVNSSDLSSIPIFLAHHGAGSTGLTFAPLAKTL 126
W+++F E V + N T F+V + S P+ L HG G + L++A +
Sbjct: 46 WSQYFESMEDVEVENE--TGKDTFRVYKSGSEG----PVLLLLHGGGHSALSWAVFTAAI 99
Query: 127 KEDLGTNFGFFSFDARGHGETKPLDPTDVTYYLNDFVTDFVELIVWFYENYLKACKQSKL 186
+ + D R HGETK +P D++ D ++ Y +
Sbjct: 100 ISRVQCRI--VALDLRSHGETKVKNPEDLS--AETMAKDVGNVVEAMYGDLPPP------ 149
Query: 187 SLILVGHSLGGSVCANLYEH--LPEYIKRHTTGLAMLDIVEEMAKFVLTKVDHFLSVTPN 244
++L+GHS+GG++ + +P + GL M+D+VE A L + +FL P
Sbjct: 150 -IMLIGHSMGGAIAVHTASSNLVPSLL-----GLCMIDVVEGTAMDALNSMQNFLRGRPK 203
Query: 245 VFGSAKEAVDWYQSHNYSKLKESAEISVPALFHKAK-------SGKVVR----------- 286
F S + A++W + ESA +S+ + + S +V
Sbjct: 204 TFKSLENAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEEEEDEE 263
Query: 287 ------------------------ITNLASFKPYWDSWFDGLSAKFVSLPTSKLLILAGN 322
LA + YWD WF GLS F+S P KLL+LAG
Sbjct: 264 GSESISKRKKEDDMETKKDHPYTWRIELAKTEKYWDGWFRGLSNLFLSCPIPKLLLLAGV 323
Query: 323 DNLDKELIVGQMQGKYQLIVFQDSGHFIQEDVPKKAAISLVDF 365
D LDK+L +GQMQGK+Q+ V GH + ED P K A ++ F
Sbjct: 324 DRLDKDLTIGQMQGKFQMQVLPQCGHAVHEDAPDKVAEAVATF 366
>7295008 [R] KOG2564 Predicted acetyltransferases and hydrolases with the
alpha/beta hydrolase fold
Length = 402
Score = 77.8 bits (190), Expect = 3e-14
Identities = 62/245 (25%), Positives = 107/245 (43%), Gaps = 22/245 (8%)
Query: 41 PVVKNPGLGVSSETKAENMEDTGFPRWTEFFAKNETVSLPNRNFTFNTY-FQVPEVNSSD 99
P + +G + K + D W EFFA+ E V++ + TF Y + PE
Sbjct: 8 PTIPGGRIGRADSFKKSRIRDYKPGMWNEFFAEKEDVTVDEQR-TFRIYRTKQPEKPG-- 64
Query: 100 LSSIPIFLAHHGAGSTGLTFAPLAKTLKEDLGTNFGFFSFDARGHGETKPLDPTDVTYYL 159
P+ L HG G + LT+A + + D RGHG++K D D++
Sbjct: 65 ----PVLLLLHGGGYSALTWAHFCSEVTSMIHCQC--LCIDMRGHGDSKVDDEDDLS--A 116
Query: 160 NDFVTDFVELIVWFYENYLKACKQSKLSLILVGHSLGGSVCANLYEHLPEYIKRHTTGLA 219
+ D +LI+ Y + L +VGHS+GG++ + + H+ + + G+
Sbjct: 117 DTLAKDIGDLILKLYPEEVP-------QLFVVGHSMGGAIAVH-FAHMA--LVPNLIGIT 166
Query: 220 MLDIVEEMAKFVLTKVDHFLSVTPNVFGSAKEAVDWYQSHNYSKLKESAEISVPALFHKA 279
++D+VE A L + FL P F S A++W + +SA++S+P
Sbjct: 167 VIDVVEGTAMEALASMQSFLRSRPKYFQSIPNAIEWCIRSGQVRNVDSAKVSMPGQIINC 226
Query: 280 KSGKV 284
+ K+
Sbjct: 227 TTNKL 231
Score = 69.7 bits (169), Expect = 7e-12
Identities = 33/71 (46%), Positives = 45/71 (62%)
Query: 289 NLASFKPYWDSWFDGLSAKFVSLPTSKLLILAGNDNLDKELIVGQMQGKYQLIVFQDSGH 348
+L+ + YW WF GLS KF++L K L+LA D LD+ L VGQMQG++Q+ V GH
Sbjct: 306 DLSKSEKYWVGWFSGLSDKFLNLRLPKQLLLASIDGLDRTLTVGQMQGRFQMQVLARCGH 365
Query: 349 FIQEDVPKKAA 359
+ ED P + A
Sbjct: 366 AVHEDRPHEVA 376
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,626,197
Number of Sequences: 60738
Number of extensions: 1091980
Number of successful extensions: 2600
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2583
Number of HSP's gapped (non-prelim): 7
length of query: 395
length of database: 30,389,216
effective HSP length: 108
effective length of query: 287
effective length of database: 23,829,512
effective search space: 6839069944
effective search space used: 6839069944
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)