ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3327 good R KOG1842 General function prediction only FYVE finger-containing protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3327 1130129 1128558 -524
(524 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR323c [R] KOG1842 FYVE finger-containing protein 407 e-113
SPAC17G6.08 [R] KOG1842 FYVE finger-containing protein 184 4e-46
Hs11641241 [R] KOG1842 FYVE finger-containing protein 84 7e-16
7297349 [R] KOG1842 FYVE finger-containing protein 84 7e-16
CE29111 [R] KOG1842 FYVE finger-containing protein 82 2e-15
7302711 [T] KOG0230 Phosphatidylinositol-4-phosphate 5-kinase an... 55 3e-07
Hs20543659 [TU] KOG1818 Membrane trafficking and cell signaling ... 54 4e-07
7301558_1 [T] KOG4381 RUN domain-containing protein 52 2e-06
Hs4758528 [TU] KOG1818 Membrane trafficking and cell signaling p... 52 2e-06
7291295 [V] KOG1841 Smad anchor for receptor activation 50 6e-06
>YDR323c [R] KOG1842 FYVE finger-containing protein
Length = 515
Score = 407 bits (1047), Expect = e-113
Identities = 209/519 (40%), Positives = 306/519 (58%), Gaps = 21/519 (4%)
Query: 21 NVEAVTCPVCGNELKSLRKLNVHLDNVHGFNXXXXXXXXXXXXXXXIGLNTLVQERTHER 80
++E V+CP+C + +L+ LN HLD HGFN L Q++
Sbjct: 2 DLENVSCPICLRKFDNLQALNAHLDVEHGFNDNEDSLGSNDSR-----LVNGKQKKARSV 56
Query: 81 QSKGKLKMNTSHWKKPVEGKSVCFECGCKLNARNGMINCRKCGELFCLRHCRNAIKLNEN 140
S + K+ SHW+K +GKS C CG LN G INCRKCG+L+C RH N IKLN +
Sbjct: 57 DSSAQ-KLKRSHWEKFKKGKSCCHTCGRTLNNNIGAINCRKCGKLYCRRHLPNMIKLNLS 115
Query: 141 GEYDPASNVWCKCCHKCYINRKGYNDYGLTRNITQAFAKIRKLKNEDGELITLQLENRLL 200
+YDP + W CCH C++ + GYNDYG ++T F K+R +K ED L LQLENR +
Sbjct: 116 AQYDPRNGKWYNCCHDCFVTKPGYNDYGEVIDLTPEFFKVRNIKREDKNLRLLQLENRFV 175
Query: 201 RLINGIIQLHVRHQSSVFATFRIRAGIIALERSIVEWKDDQMATQCGICSQPFGLLMRKH 260
RL++G+I L+ + S+ ++ + + LER++ W+DD+ C ICS+PFGLL+RKH
Sbjct: 176 RLVDGLITLYNTYSRSIIHNLKMNSEMSKLERTVTPWRDDRSVLFCNICSEPFGLLLRKH 235
Query: 261 HCRLCGQIVCDRQETRCSSDIPVLNLMSAAPDLSFENVGNLKAISDLSTGVRICSSCLRY 320
HCRLCG +VCD CS++I + LMSAA DL FE + + +R+CS C+
Sbjct: 236 HCRLCGMVVCDDANRNCSNEISIGYLMSAASDLPFEYNIQKDDLLHIPISIRLCSHCIDM 295
Query: 321 VFAKRKLQFSKALKLPEILQQYENLHNLETLVVNLLPRFQKALDSIHNAPASDE------ 374
+F RK + L I +Y+++ N+ ++ +LLP F+ +L+S+ A D
Sbjct: 296 LFIGRKFNKDVRMPLSGIFAKYDSMQNISKVIDSLLPIFEDSLNSLKVETAKDSENTLDP 355
Query: 375 TAIQNLAQLRRKLLQNLTLYDKLSKSIVQLDTKSTAEKKIQQSIALHASAFIEEKMLPLK 434
+ +LA+LR KLL + LY+ L++ ++ ++ +S E+++Q SI + ++A+I EK+LPLK
Sbjct: 356 KNLNDLARLRHKLLNSFNLYNTLTRQLLSVEPQSHLERQLQNSIKIASAAYINEKILPLK 415
Query: 435 KIPEILNPVSQKV---------EVTTSSDLLFNNLTIAEVKKYREELMVLNEQRYLVENM 485
+P ILNP K EV S L+ NLTI EVK+ REELMVL EQ YL+E+
Sbjct: 416 SLPAILNPEGHKTNEDGQKAEPEVKKLSQLMIENLTIKEVKELREELMVLKEQSYLIEST 475
Query: 486 LNSAAKERRFEEAISLKTNLSEIDQNIVHLESKLGDEGF 524
+ K+RR EE ++L NL E+ I ++SKLGD GF
Sbjct: 476 IQDYKKQRRLEEIVTLNKNLEELHSRIHTVQSKLGDHGF 514
>SPAC17G6.08 [R] KOG1842 FYVE finger-containing protein
Length = 536
Score = 184 bits (466), Expect = 4e-46
Identities = 149/542 (27%), Positives = 233/542 (42%), Gaps = 67/542 (12%)
Query: 11 SASSTGQHDRNVEAVTCPVCGNELKSLRKLNVHLDNVH------------GFNXXXXXXX 58
S S+ V+A CP+CG EL +L LN HLD H N
Sbjct: 25 STSAQSDSSNIVKATECPICGLELPNLSALNDHLDVTHFNDNEKIHKRQDSINSWLTRTL 84
Query: 59 XXXXXXXXIGLNTLVQERTHERQ--SKGKLKMNTS----------HWKKPVEGKSVCFE- 105
L + +E+ S G + + + HW+ V VC +
Sbjct: 85 NGASALQMKAAQRLWRMEPYEQNGDSSGAVGLEATKLTDSLVVKNHWQPEVPDM-VCHDP 143
Query: 106 -CGCKLNARNGMINCRKCGELFCLRHCRNAIKLNENGEYDPASNVWCKCCHKCYINRKGY 164
C LN NG I+CRKCG +FC H IKL+ + YD + WC+ C +CY R GY
Sbjct: 144 MCDKLLNFINGHIHCRKCGYIFCNFHSMYQIKLSIHATYDSENGFWCRVCRECYEGRPGY 203
Query: 165 ND-YGLTRNITQAFAKIRKLKNEDGELITLQLENRLLRLINGIIQLHVRHQSSVFATFRI 223
ND GL R+ Q F RK + + L+L R+ +L +L S+ +
Sbjct: 204 NDSNGLIRSRFQTFETFRKPLADKRRIEFLRLSKRMKKL----EELWTSENVSMLDALLL 259
Query: 224 RAGIIALERSIVEWKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDRQETRCSSDIPV 283
LE+SIV W+DD + C C+ F L R+ HCRLCG+++C C +I
Sbjct: 260 NKA-KRLEQSIVHWQDDSVVQICPECNNSFTLTRRRRHCRLCGRVIC----RFCVLEI-- 312
Query: 284 LNLMSAAPDLSFENVGNLKAISDLSTGVRICSSCLRYVFAKRKLQFSKALKLPEILQQYE 343
++ + IC SC + F Q ++ L I + E
Sbjct: 313 -------------------SLPQHPQPLLICMSCNQNYFRNVLYQTERSKSLGYI-RHIE 352
Query: 344 NLHNLETLVVNLLPRFQKALDSIHNAPASDETAIQNLAQLRRKLLQNLTLYDKLSKSIVQ 403
+L +VN ++ +L + + E ++ + R+K L+ YD K I
Sbjct: 353 HLQVFRQAMVNYYRLYEDSLSELLSGEIITEATLKIVKDRRKKFLELCVKYDGTMKKIAN 412
Query: 404 LDTKSTAEKKIQQSIALHASAFIEEKMLPLKKIPEILNPVSQKVEVTTSSDLLFNNLTIA 463
+ + AE++ +Q++ A +++E +L L+ IP L Q + TS
Sbjct: 413 HPSSNDAEEQFKQNVVNEAKRYLQETILRLQAIPYHL----QVGQAWTSESERELEKKKE 468
Query: 464 EVKKYREELM----VLNEQRYLVENMLNSAAKERRFEEAISLKTNLSEIDQNIVHLESKL 519
+V+K +EELM VL EQ +LVENM+ A +R+F E +L ++L+ + + I + K+
Sbjct: 469 QVEKKQEELMQTRIVLEEQVFLVENMIEDAKAKRKFSEVETLLSSLAPLHEEIHSITEKI 528
Query: 520 GD 521
D
Sbjct: 529 HD 530
>Hs11641241 [R] KOG1842 FYVE finger-containing protein
Length = 784
Score = 83.6 bits (205), Expect = 7e-16
Identities = 66/242 (27%), Positives = 117/242 (48%), Gaps = 35/242 (14%)
Query: 225 AGIIALERSIVEWKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDRQETRCSSDIPVL 284
A I A+E+S+V W +DQ C C F + R+HHCRLCG I+C + S +P+
Sbjct: 142 AKIRAIEKSVVPWVNDQDVPFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELIS--LPLA 199
Query: 285 NLMSAA----------PDLSFENV-----GNLKAISDLST--------GVRICSSCLRYV 321
N +++A P S +V G++ ++S +S+ +R C+ C +
Sbjct: 200 NKLTSASKESLSTHTSPSQSPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHC-KDT 258
Query: 322 FAKRKLQFSKALKLPEILQQYENLHNLETLVVNLLPRFQKALDSIHNAPASDET-AIQNL 380
KR+ Q + P+I++ YE L V P + + S++ A + T ++++
Sbjct: 259 LLKREQQIDEKEHTPDIVKLYEKLRLCMEKVDQKAPEYIRMAASLN---AGETTYSLEHA 315
Query: 381 AQLRRKLLQNLTLYDKLSKSIVQLDTKS-----TAEKKIQQSIALHASAFIEEKMLPLKK 435
+ LR ++ + L D LSK I+ L + ++Q+ I A+ F++EK+L L
Sbjct: 316 SDLRVEVQKVYELIDALSKKILTLGLNQDPPPHPSNLRLQRMIRYSATLFVQEKLLGLMS 375
Query: 436 IP 437
+P
Sbjct: 376 LP 377
>7297349 [R] KOG1842 FYVE finger-containing protein
Length = 505
Score = 83.6 bits (205), Expect = 7e-16
Identities = 66/264 (25%), Positives = 117/264 (44%), Gaps = 32/264 (12%)
Query: 231 ERSIVEWKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDRQETRCSSDIPVLNLMSAA 290
E+ V W D C C++ F + R+HHCRLCG I+C+ CS +P+ + M A
Sbjct: 171 EQQTVAWLDGSSVKLCPSCAKSFHIARRQHHCRLCGGIMCN----DCSKFLPLEDAMQLA 226
Query: 291 PDLSFENVGNLKAISDLSTGVRICSSCLRYVFAKRKLQFSKALKLPEILQQYENLHNLET 350
LS L+ + +R+C CL + ++ + S+ + P ++Q YE + L+
Sbjct: 227 -SLSTTRSEPLQQLHQHENAIRLCEHCLWLLDTRKDMHESRTCR-PLLVQVYEQIRQLQK 284
Query: 351 LVVNLLPRFQKALDSIHNAPASDETAIQNLAQLRRKLLQNLTLYDKLSKSIVQLDTK-ST 409
V L + K ++S++ + + LR K+ Q D SK I+ + + +
Sbjct: 285 EVTPDLDMYLKIINSLNEGDTI--FTLADAGALRGKIGQVAEAMDMRSKRILAIFCEPGS 342
Query: 410 AEKKIQQSIALHASAFIEEKMLPLKKIPEILNPVSQKVEVTTSSDLLFNNLTIAEVKKYR 469
E+ ++++I L I+E+ML L +PE + +++ +
Sbjct: 343 REEALKKAIRLGCIQAIKERMLSLPPLPE-----------------------ESHIRQMQ 379
Query: 470 EELMVLNEQRYLVENMLNSAAKER 493
E + EQR L E + A ER
Sbjct: 380 ERRRMETEQRILTEQRMAMEAYER 403
>CE29111 [R] KOG1842 FYVE finger-containing protein
Length = 563
Score = 82.4 bits (202), Expect = 2e-15
Identities = 101/417 (24%), Positives = 172/417 (41%), Gaps = 86/417 (20%)
Query: 168 GLTRNITQAFAKIRKLKNEDGELITLQLENRLLRLINGIIQLHVRHQSSVFATFRIRAGI 227
G R+ T+ F R+L + + T L RL RLING + + F
Sbjct: 119 GTRRSKTREFLSTRELNINEVAVRTNMLIIRLDRLINGGPKDKDPVKRKEF--------- 169
Query: 228 IALERSIVEWKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVC---------DRQETRCS 278
ER +V W DD A C +C+ FGL R+HHCRLCG+++C D +T S
Sbjct: 170 ---EREVVPWLDDAEAVCCPLCASRFGLTRRRHHCRLCGRVLCHSCSKFLSFDTAKTLTS 226
Query: 279 SDIPV--LNLMSAAPD---------------------LSFENVGNLKAI----------- 304
S P+ +++ P+ LS +++ +K
Sbjct: 227 SSNPLGDTHIIVEEPNTVENEQELLQPEENGRRSFFSLSQKSMDKMKRAMAGAVQKVHSA 286
Query: 305 -------------SDLSTGVRICSSCLRYVFAKRKLQFSKALKLPEILQQYENLHNLETL 351
DLS +R+C+ CLR + + K + PE +QQY+ + +
Sbjct: 287 ATGEEIIAGALNEQDLSEHMRVCNLCLRDLHFRESQMDKK--EPPEFVQQYDLMKVMIEE 344
Query: 352 VVNLLPRFQKALDSIHNAPASDETAIQNLAQLRRKLLQNLTLYDKLSKSIVQ---LDTKS 408
V NL+P + + + S+ N + ++ +LR K L+ D +SK I + S
Sbjct: 345 VKNLVPTYVRVVTSLKN--GESVYSHKHGEELRSKCLEIQKSIDYVSKKIAEGANDKPNS 402
Query: 409 TAEKKIQQSIALHASAFIEEKMLPLKKIP---EILNPVSQKVEVTTSSDLLFNNLTIAEV 465
E++++++I L+A ++ + ++ +P E N + ++ E + D N T V
Sbjct: 403 AKEQQLRKNIRLYAVQALQGLIGQMESLPTAEECSNLIIKRKE-RRAKDFARVNRT---V 458
Query: 466 KKYREELMVLNEQRYLVENMLNSAAKERRFEEAISLKTNLSEIDQNIVHLESKLGDE 522
K + L EQ + LNS + F S+ S D ++V S + D+
Sbjct: 459 MKMSSSMPQLQEQ----HSHLNSTSSVSSFGGPSSIDDGWSAEDNDLVFEPSSIEDD 511
>7302711 [T] KOG0230 Phosphatidylinositol-4-phosphate 5-kinase and related
FYVE finger-containing proteins
Length = 1809
Score = 54.7 bits (130), Expect = 3e-07
Identities = 26/60 (43%), Positives = 29/60 (48%), Gaps = 8/60 (13%)
Query: 237 WKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDR--------QETRCSSDIPVLNLMS 288
W D A +C CSQ F RKHHCRLCGQI C + RC D+ V N S
Sbjct: 183 WMPDSKAKECYDCSQKFSTFRRKHHCRLCGQIFCSKCCNQVVPGMIIRCDGDLKVCNYCS 242
>Hs20543659 [TU] KOG1818 Membrane trafficking and cell signaling protein HRS
contains VHS and FYVE domains
Length = 777
Score = 54.3 bits (129), Expect = 4e-07
Identities = 43/178 (24%), Positives = 65/178 (36%), Gaps = 30/178 (16%)
Query: 103 CFECGCKLNARNGMINCRKCGELFCLRHCRNAIKLNENGEYDPASNVWCKCCHKCYINRK 162
C +C + +CR CGE FC + E G + PA + C CY
Sbjct: 604 CNKCATSFKDNDTKHHCRACGEGFCDSCSSKTRPVPERG-WGPAP---VRVCDNCYE--- 656
Query: 163 GYNDYGLTRNITQAFAKIRKLKNEDGELITLQLENRLLRLINGIIQLHVRHQSSVFATFR 222
RN+ A + + + +E G LI ++ + + ++
Sbjct: 657 -------ARNVQLAVTEAQ-VDDEGGTLIARKVGEAVQNTLGAVV-----------TAID 697
Query: 223 IRAGIIALERSIVEWKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDRQETRCSSD 280
I G++ W D C C + F + + KHHCR CGQ CD CS D
Sbjct: 698 IPLGLVKDAARPAYWVPDHEILHCHNCRKEFSIKLSKHHCRACGQGFCD----ECSHD 751
>7301558_1 [T] KOG4381 RUN domain-containing protein
Length = 951
Score = 52.4 bits (124), Expect = 2e-06
Identities = 30/89 (33%), Positives = 42/89 (46%), Gaps = 7/89 (7%)
Query: 237 WKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDRQETRCSS-DIPVLNLMSAAPDLSF 295
W D +AT C C + F L RKHHCR CG+I C CS +P+LN
Sbjct: 860 WAPDSIATHCTACEREFNLTRRKHHCRSCGEIFC----KACSEHTLPLLNAQGQPGKPVR 915
Query: 296 ENVGNL--KAISDLSTGVRICSSCLRYVF 322
+G+L S +G +I SC+ ++
Sbjct: 916 VLLGSLGRSCCSSAKSGFKIQPSCISKIW 944
>Hs4758528 [TU] KOG1818 Membrane trafficking and cell signaling protein HRS
contains VHS and FYVE domains
Length = 777
Score = 52.0 bits (123), Expect = 2e-06
Identities = 37/123 (30%), Positives = 52/123 (42%), Gaps = 26/123 (21%)
Query: 214 QSSVFATFRIRAGIIALERSIVEWKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCDRQ 273
+ VF F+ + A ER+ +W D A +C C FG++ RKHHCR CGQI C
Sbjct: 138 EGHVFPEFKESDAMFAAERA-PDWVD---AEECHRCRVQFGVMTRKHHCRACGQIFCG-- 191
Query: 274 ETRCSSDIPVLNLMSAAPDLSFENVGNLKAISDLSTGVRICSSCLRYVFAKRKLQFSKAL 333
+CSS S P E VR+C C + K + + +
Sbjct: 192 --KCSSK------YSTIPKFGIEK------------EVRVCEPCYEQLNRKAEGKATSTT 231
Query: 334 KLP 336
+LP
Sbjct: 232 ELP 234
>7291295 [V] KOG1841 Smad anchor for receptor activation
Length = 1291
Score = 50.4 bits (119), Expect = 6e-06
Identities = 27/73 (36%), Positives = 34/73 (45%), Gaps = 7/73 (9%)
Query: 237 WKDDQMATQCGICSQPFGLLMRKHHCRLCGQIVCD-------RQETRCSSDIPVLNLMSA 289
W D MA QC C Q F ++ R+HHCR CG+++C R E + V N A
Sbjct: 528 WVPDNMAGQCMQCQQKFTMIKRRHHCRACGKVLCSVCCSQRFRLEFATEPESRVANSPGA 587
Query: 290 APDLSFENVGNLK 302
P VG LK
Sbjct: 588 PPPSVIVPVGVLK 600
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.135 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,238,968
Number of Sequences: 60738
Number of extensions: 1194406
Number of successful extensions: 4836
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 4799
Number of HSP's gapped (non-prelim): 28
length of query: 524
length of database: 30,389,216
effective HSP length: 111
effective length of query: 413
effective length of database: 23,647,298
effective search space: 9766334074
effective search space used: 9766334074
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)