ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3445 good J KOG2523 Translation, ribosomal structure and biogenesis Predicted RNA-binding protein with PUA domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3445 Highly similar to YER007ca singleton [INTRON]
(158 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER007c-a [J] KOG2523 Predicted RNA-binding protein with PUA domain 261 2e-70
SPBC31F10.12 [J] KOG2523 Predicted RNA-binding protein with PUA ... 176 2e-44
Hs7662502 [J] KOG2523 Predicted RNA-binding protein with PUA domain 154 6e-38
7290666 [J] KOG2523 Predicted RNA-binding protein with PUA domain 147 8e-36
CE16845_2 [J] KOG2523 Predicted RNA-binding protein with PUA domain 145 3e-35
At1g09150 [J] KOG2523 Predicted RNA-binding protein with PUA domain 134 7e-32
Hs11386191 [J] KOG2522 Filamentous baseplate protein Ligatin con... 64 1e-10
ECU05g1350 [J] KOG2523 Predicted RNA-binding protein with PUA do... 63 1e-10
At1g71350 [J] KOG2522 Filamentous baseplate protein Ligatin cont... 45 4e-05
CE27093 [J] KOG2522 Filamentous baseplate protein Ligatin contai... 35 0.042
ECU11g2000 [BDL] KOG0979 Structural maintenance of chromosome pr... 31 0.80
SPCC584.05 [U] KOG1300 Vesicle trafficking protein Sec1 28 4.0
Hs4505285 [WV] KOG1216 von Willebrand factor and related coagula... 28 4.0
YHR128w [TZ] KOG4203 Armadillo/beta-Catenin/plakoglobin 28 6.8
CE13842 [I] KOG1176 Acyl-CoA synthetase 28 6.8
At5g39040 [U] KOG0058 Peptide exporter ABC superfamily 27 8.8
7293415 [Z] KOG3595 Dyneins heavy chain 27 8.8
7299131_1 [T] KOG4625 Notch signaling protein Neuralized Nuez do... 27 8.8
>YER007c-a [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 181
Score = 261 bits (668), Expect = 2e-70
Identities = 122/158 (77%), Positives = 142/158 (89%)
Query: 1 MKAKLLAQYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVNGEVLFFQNFDELIPSLRFV 60
+KAKL+ QYPK+E+VIDE+IPKK+QIEL KCE KIQLY V+GEVLFFQ FDELIPSL+ V
Sbjct: 24 LKAKLVKQYPKIEDVIDELIPKKSQIELIKCEDKIQLYSVDGEVLFFQKFDELIPSLKLV 83
Query: 61 HKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKETA 120
HKFPEA+PTVQVDRGAIKFVL+G+NIMCPGLTS GA LP APG+EK TIVVINAENKE A
Sbjct: 84 HKFPEAYPTVQVDRGAIKFVLSGANIMCPGLTSAGADLPPAPGYEKGTIVVINAENKENA 143
Query: 121 MAVGKLLMSTEEIKEINKGHGVEMIHHLGDPLWTFSAD 158
+A+G+L+M TEEIK +NKGH +E+IHHLGDPLW FS +
Sbjct: 144 LAIGELMMGTEEIKSVNKGHSIELIHHLGDPLWNFSVE 181
>SPBC31F10.12 [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 184
Score = 176 bits (445), Expect = 2e-44
Identities = 86/154 (55%), Positives = 114/154 (73%), Gaps = 3/154 (1%)
Query: 1 MKAKLLAQYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVNGEVLFFQNFD-ELIPSLRF 59
+KAKL+ YP L++VIDE+IPKK+Q+ KCE ++ LY +NGE++ FQ+FD +IPSLR
Sbjct: 28 IKAKLVQAYPNLKQVIDELIPKKSQLTQIKCEDRLFLYTLNGEIILFQHFDGPIIPSLRL 87
Query: 60 VHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKET 119
VHK P+AF V+VDRGAIKF+L+G+NIM PGL S G LP+ EKD V++ AE KE
Sbjct: 88 VHKCPDAFTQVRVDRGAIKFLLSGANIMIPGLVSKGGNLPD--DIEKDQYVIVTAEGKEA 145
Query: 120 AMAVGKLLMSTEEIKEINKGHGVEMIHHLGDPLW 153
A+G MS +E+KE NKG G+E +H+LGD LW
Sbjct: 146 PAAIGLTKMSAKEMKETNKGIGIENVHYLGDNLW 179
>Hs7662502 [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 181
Score = 154 bits (388), Expect = 6e-38
Identities = 78/154 (50%), Positives = 109/154 (70%), Gaps = 4/154 (2%)
Query: 1 MKAKLLAQYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVNGEVLFF-QNFDELIPSLRF 59
+K +L+ Q+P +E +++I+PKK +++ +C I++ VNGE+LFF Q P+LR
Sbjct: 25 IKNQLIEQFPGIEPWLNQIMPKKDPVKIVRCHEHIEILTVNGELLFFRQREGPFYPTLRL 84
Query: 60 VHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKET 119
+HK+P P QVD+GAIKFVL+G+NIMCPGLTSPGAKL A DTIV I AE K+
Sbjct: 85 LHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPA---AVDTIVAIMAEGKQH 141
Query: 120 AMAVGKLLMSTEEIKEINKGHGVEMIHHLGDPLW 153
A+ VG + MS E+I+++NKG G+E IH+L D LW
Sbjct: 142 ALCVGVMKMSAEDIEKVNKGIGIENIHYLNDGLW 175
>7290666 [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 182
Score = 147 bits (370), Expect = 8e-36
Identities = 81/155 (52%), Positives = 105/155 (67%), Gaps = 5/155 (3%)
Query: 1 MKAKLLAQYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVN-GEVLFFQNFD-ELIPSLR 58
++AKLL YPKLE ID I+PKK + KC I+L L G+ +FF++ D +P+LR
Sbjct: 25 IRAKLLEAYPKLESHIDLILPKKDSYRIAKCHDHIELLLNGAGDQVFFRHRDGPWMPTLR 84
Query: 59 FVHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKE 118
+HKFP QVD+GAI+FVL+G+N+MCPGLTSPGA + A +KDT+V I AE KE
Sbjct: 85 LLHKFPYFVTMQQVDKGAIRFVLSGANVMCPGLTSPGACMTPA---DKDTVVAIMAEGKE 141
Query: 119 TAMAVGKLLMSTEEIKEINKGHGVEMIHHLGDPLW 153
A+AVG L +ST+EI NKG G+E H L D LW
Sbjct: 142 HALAVGLLTLSTQEILAKNKGIGIETYHFLNDGLW 176
>CE16845_2 [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 180
Score = 145 bits (365), Expect = 3e-35
Identities = 74/155 (47%), Positives = 109/155 (69%), Gaps = 4/155 (2%)
Query: 1 MKAKLLAQYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVN-GEVLFFQNFD-ELIPSLR 58
++ KL+ +P LE ++EI+PKK ++ KC+ I+L + G V F + + + IP+LR
Sbjct: 20 IRKKLIENFPYLEPHLEEILPKKENFKVIKCKDHIELLADHLGVVRFVKTRNTDYIPTLR 79
Query: 59 FVHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKE 118
+HK+P P QVD+GAIKF+L GS+IMCPGLTSPGAKL P KD++V + AE K+
Sbjct: 80 TLHKYPFILPHQQVDKGAIKFILNGSSIMCPGLTSPGAKL--TPLVPKDSVVAVMAEGKQ 137
Query: 119 TAMAVGKLLMSTEEIKEINKGHGVEMIHHLGDPLW 153
A+A+G + MS+EEI+ +NKG+G+E +H+L D LW
Sbjct: 138 HALAIGLMSMSSEEIQSVNKGNGIENLHYLNDGLW 172
>At1g09150 [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 181
Score = 134 bits (336), Expect = 7e-32
Identities = 70/147 (47%), Positives = 97/147 (65%), Gaps = 3/147 (2%)
Query: 8 QYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVNGEVLFFQNFD-ELIPSLRFVHKFPEA 66
+YP LE V+++++PKK + + KC + L +VN LFF D +P+LR +H++P
Sbjct: 31 EYPGLESVMEDLLPKKIPLIVVKCPNHLTLVVVNNVPLFFCIRDGPYMPTLRLLHQYPNI 90
Query: 67 FPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKETAMAVGKL 126
QVDRGAIKFVL+G+NIMCPGLTSPG L + E + V I AE K+ A+A+G
Sbjct: 91 MKRFQVDRGAIKFVLSGANIMCPGLTSPGGVLDQE--VEAERPVAIYAEGKQHALAIGFT 148
Query: 127 LMSTEEIKEINKGHGVEMIHHLGDPLW 153
MS ++IK INKG GV+ +H+L D LW
Sbjct: 149 KMSAKDIKSINKGIGVDNMHYLNDGLW 175
>Hs11386191 [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 584
Score = 63.5 bits (153), Expect = 1e-10
Identities = 41/159 (25%), Positives = 79/159 (48%), Gaps = 9/159 (5%)
Query: 1 MKAKLLAQYPKL-EEVIDEIIPKKAQ---IELYKCEGK-IQLYLVNGEVLFFQNFDELIP 55
++A + +P L + + E++P K + ++LY +G + +Y+ G + F+ L P
Sbjct: 22 LRADVTTAFPTLGTDQVSELVPGKEELNIVKLYAHKGDAVTVYVSGGNPILFELEKNLYP 81
Query: 56 SLRFVHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAE 115
++ + +P+ PT ++ ++ G+++M PGL P A LP+ +K + I+
Sbjct: 82 TVYTLWSYPDLLPTFTTWPLVLEKLVGGADLMLPGLVMPPAGLPQV---QKGDLCAISLV 138
Query: 116 NKETAMAVGKLLMSTEEIKEIN-KGHGVEMIHHLGDPLW 153
+A+G MST E+ KG G ++H D LW
Sbjct: 139 GNRAPVAIGVAAMSTAEMLTSGLKGRGFSVLHTYQDHLW 177
>ECU05g1350 [J] KOG2523 Predicted RNA-binding protein with PUA domain
Length = 203
Score = 63.2 bits (152), Expect = 1e-10
Identities = 34/137 (24%), Positives = 74/137 (53%), Gaps = 3/137 (2%)
Query: 18 EIIPKKAQIELYKCEGKIQLYLVNGEVLFFQNFDELIPSLRFVHKFPEAFPTVQVDRGAI 77
E++ K +++C+ K L + G + F + P++R++ F V +D GA+
Sbjct: 66 EVLEKNETYMVHRCKNKASLISLGGSAILFCLNRKYFPTIRYLEGHGGGFYEVFLDEGAL 125
Query: 78 KFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKETAMAVGKLLMSTEEIKEIN 137
+ + G+++M PG+ + + GFE +V+ K + +AVG+ ++ ++++ +
Sbjct: 126 EPLCRGADVMIPGVLKYKDLIKK--GFEAGDTIVVRIIGK-SIVAVGEAIVGLKDMEASS 182
Query: 138 KGHGVEMIHHLGDPLWT 154
+G G+E+ H +GD L+T
Sbjct: 183 RGVGIEVYHRVGDGLYT 199
>At1g71350 [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 566
Score = 45.1 bits (105), Expect = 4e-05
Identities = 37/142 (26%), Positives = 59/142 (41%), Gaps = 22/142 (15%)
Query: 13 EEVIDEIIPKKAQIELYKCEGKIQLYLVNGEVLFFQNFDELIPSLRFVHKFPEAFPTVQV 72
+E++D I+P K +I L K + ++ +Y + G F + D + E FPTV
Sbjct: 35 DELLDAILPPKVEITLSKFQNRVIVYSIEGGCPMFFDIDG---------RGTEIFPTV-- 83
Query: 73 DRGAIKFVLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKETAMAVGKLLMSTEE 132
F L + M P G P F I + +AVG MS+EE
Sbjct: 84 ------FALWEAPEMLPSFMLKGGY----PSFSAGEIWAVKVPGNLAPIAVGCTTMSSEE 133
Query: 133 IKEIN-KGHGVEMIHHLGDPLW 153
++ +G + + H+ D LW
Sbjct: 134 ARKAGLRGKALRITHYYRDFLW 155
>CE27093 [J] KOG2522 Filamentous baseplate protein Ligatin contains PUA
domain
Length = 485
Score = 35.0 bits (79), Expect = 0.042
Identities = 32/137 (23%), Positives = 60/137 (43%), Gaps = 35/137 (25%)
Query: 24 AQIELYKCEG-KIQLYLVNGEVLFFQNFDE---LIPSLRFVHKFPEAFPTVQVDRGAIKF 79
AQ++L EG ++ +Y ++ + F+ F E + P++ ++ + FP + +
Sbjct: 43 AQVKLTTFEGTQMNVYTIDKVPMLFE-FSENGNIYPTVYYMWNNQKTFPVLICHEPVFSY 101
Query: 80 VLAGSNIMCPGLTSPGAKLPEAPGFEKDTIVVINAENKETAMAVGKLLMSTEEIKEIN-K 138
+ G+++M PG + AVG +MS+EE+ K
Sbjct: 102 LENGADLMLPGPS-----------------------------AVGCSMMSSEEMVVCGFK 132
Query: 139 GHGVEMIHHLGDPLWTF 155
G GV+++H D LW F
Sbjct: 133 GKGVQVLHVFRDQLWDF 149
>ECU11g2000 [BDL] KOG0979 Structural maintenance of chromosome protein
SMC5/Spr18 SMC superfamily
Length = 1025
Score = 30.8 bits (68), Expect = 0.80
Identities = 19/50 (38%), Positives = 32/50 (64%), Gaps = 2/50 (4%)
Query: 4 KLLAQYPK-LEEVIDEIIPKKAQIELYKCE-GKIQLYLVNGEVLFFQNFD 51
K+LAQ P ++E+ DEII ++AQ++LY + G ++ + V + L N D
Sbjct: 792 KILAQLPDTIDELDDEIIKERAQLKLYNVDRGAVEQFEVREQDLRSLNKD 841
>SPCC584.05 [U] KOG1300 Vesicle trafficking protein Sec1
Length = 693
Score = 28.5 bits (62), Expect = 4.0
Identities = 25/106 (23%), Positives = 44/106 (40%), Gaps = 6/106 (5%)
Query: 33 GKIQLYLVNGEVLFFQNFDELIPSLRFVHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGLT 92
G Y N + FF F + +L+F P P ++ R + + NI P
Sbjct: 574 GSTVCYSPNEWLDFFSKFQQPREALKFPEDKPRHIPCLEA-RPSPNRIQEQPNIDVPSTR 632
Query: 93 SPGAKLPEAPGFEKDTIVVINAENKETAMAVGKLLMSTEEIKEINK 138
+A KD ++K + +GKL+MS+++ K+ +K
Sbjct: 633 YENTSSSDAVSIHKD-----EKKSKGKSHKLGKLMMSSKKDKDKSK 673
>Hs4505285 [WV] KOG1216 von Willebrand factor and related coagulation proteins
Length = 5179
Score = 28.5 bits (62), Expect = 4.0
Identities = 14/35 (40%), Positives = 21/35 (60%)
Query: 69 TVQVDRGAIKFVLAGSNIMCPGLTSPGAKLPEAPG 103
T +VD + V+A +++ C SPG +L EAPG
Sbjct: 4950 TDKVDNNTLLNVIACTHVPCNTSCSPGFELMEAPG 4984
>YHR128w [TZ] KOG4203 Armadillo/beta-Catenin/plakoglobin
Length = 251
Score = 27.7 bits (60), Expect = 6.8
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Query: 40 VNGEVLFFQNFDELIPSLRFVHKFPEAFPTVQVDRGAIKFVLAGSNIMCPGL 91
V E ++F N LI S + K+ AFP V++ GA+ L + + PGL
Sbjct: 193 VKPERIYFLN---LICSKEGIEKYHAAFPEVRIVTGALDRGLDENKYLVPGL 241
>CE13842 [I] KOG1176 Acyl-CoA synthetase
Length = 554
Score = 27.7 bits (60), Expect = 6.8
Identities = 17/75 (22%), Positives = 33/75 (43%)
Query: 6 LAQYPKLEEVIDEIIPKKAQIELYKCEGKIQLYLVNGEVLFFQNFDELIPSLRFVHKFPE 65
L Y ++E + +PK A++ + E ++ L ++ +N DEL+ H E
Sbjct: 461 LQSYNFVQENVILTLPKVAEVAVVGIEDELCGQLPKAYIVLEKNADELLFLKHLEHTMKE 520
Query: 66 AFPTVQVDRGAIKFV 80
V+ RG + +
Sbjct: 521 KLSAVKQLRGGVSII 535
>At5g39040 [U] KOG0058 Peptide exporter ABC superfamily
Length = 639
Score = 27.3 bits (59), Expect = 8.8
Identities = 21/83 (25%), Positives = 39/83 (46%), Gaps = 8/83 (9%)
Query: 1 MKAKLLAQYPKLEEVIDEIIPKKAQIE-----LYKCEGKIQL-YLVNGEVLF--FQNFDE 52
+K K+L L E+ + + K+ I L+ C + + Y +GE F +N +
Sbjct: 446 LKGKILLNGVSLMEISHQYLHKQISIVSQEPILFNCSVEENIAYGFDGEASFTDIENAAK 505
Query: 53 LIPSLRFVHKFPEAFPTVQVDRG 75
+ + F+ FP+ + TV +RG
Sbjct: 506 MANAHEFIEAFPDKYNTVVGERG 528
>7293415 [Z] KOG3595 Dyneins heavy chain
Length = 4081
Score = 27.3 bits (59), Expect = 8.8
Identities = 32/124 (25%), Positives = 55/124 (43%), Gaps = 8/124 (6%)
Query: 9 YPKLEEVIDEIIPKKA----QIELYKCEGKIQLYLVNGEVLFFQNFDELIPSLRFVHKFP 64
YP L E I+E I Q E Y+ EG+ L L + + + NF +L + + + P
Sbjct: 3055 YPVLLEEINETIDPSLRPILQRETYRFEGRTYLKLGDMVIDYDDNF-KLYMTTKLPN--P 3111
Query: 65 EAFPTVQVDRGAIKFVLAGSNIMCPGLTSPGA-KLPEAPGFEKDTIVVINAENKETAMAV 123
P V ++ + F++ S + L A +LP D +V IN++ ++
Sbjct: 3112 HYLPEVCINVTLVNFLVTESGLEDQLLADIVAIELPAMEIQRNDLVVKINSDKQQLLALE 3171
Query: 124 GKLL 127
K+L
Sbjct: 3172 DKVL 3175
>7299131_1 [T] KOG4625 Notch signaling protein Neuralized Nuez domain
Length = 700
Score = 27.3 bits (59), Expect = 8.8
Identities = 13/53 (24%), Positives = 25/53 (46%), Gaps = 2/53 (3%)
Query: 97 KLPEAPGFEKDTIVVINAENKET--AMAVGKLLMSTEEIKEINKGHGVEMIHH 147
+LP + V +NA + A + ++L TE + +N G +++HH
Sbjct: 524 QLPNMVAYPSQPQVNVNASSSSACNAASTSRMLPMTESMSSLNAGATAKLLHH 576
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.139 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,732,244
Number of Sequences: 60738
Number of extensions: 390282
Number of successful extensions: 986
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 960
Number of HSP's gapped (non-prelim): 22
length of query: 158
length of database: 30,389,216
effective HSP length: 97
effective length of query: 61
effective length of database: 24,497,630
effective search space: 1494355430
effective search space used: 1494355430
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)