ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3507 good O KOG2754 Posttranslational modification, protein turnover, chaperones Oligosaccharyltransferase, beta subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3507 1194407 1195702 432
(432 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YEL002c [O] KOG2754 Oligosaccharyltransferase beta subunit 412 e-115
SPCC338.15 [O] KOG2754 Oligosaccharyltransferase beta subunit 158 2e-38
Hs20070197 [O] KOG2754 Oligosaccharyltransferase beta subunit 144 3e-34
7291015 [O] KOG2754 Oligosaccharyltransferase beta subunit 135 1e-31
At5g66680 [O] KOG2754 Oligosaccharyltransferase beta subunit 122 8e-28
CE01081 [O] KOG2754 Oligosaccharyltransferase beta subunit 115 1e-25
>YEL002c [O] KOG2754 Oligosaccharyltransferase beta subunit
Length = 430
Score = 412 bits (1059), Expect = e-115
Identities = 202/413 (48%), Positives = 285/413 (68%), Gaps = 6/413 (1%)
Query: 12 FFLALGAVNALSVHGLKTLVIYDNRVTDLDNYSKFLDSLKQRSYDIQLASVNEDAEVLSL 71
F + L A+ + +TLV+YD L+ YS +L L+QR+Y ++ +N + + L
Sbjct: 9 FCILLQAIFVVGTQTSRTLVLYDQSTEPLEEYSVYLKDLEQRNYKLEYLDINSTSTTVDL 68
Query: 72 YNNDERLYDNLIIFPLKT-RQISKQISTSKLVQFHTEGGDIMAITSPEGLVDPVRLFLNE 130
Y+ ++RL+DN+I+FP K + +++QI +L++F G+I+ ++SP + + +RLFLNE
Sbjct: 69 YDKEQRLFDNIIVFPTKGGKNLARQIPVKQLIKFFENEGNILCMSSPGAVPNTIRLFLNE 128
Query: 131 LGIYPSPKNFLLKNFNENGPSGSHEISTSN-ILNRYVYDGSREFELNY-EGSAALLDNGE 188
LGIYPSPK +++++ PS + +SN +LN+YVY+ + + + E SAALL+N E
Sbjct: 129 LGIYPSPKGHVIRDYFS--PSSEELVVSSNHLLNKYVYNARKSEDFVFGESSAALLENRE 186
Query: 189 YIVEILRAPRESVCKDINNKEEDWSVGSQGHLIAGFQTLNSARTTWIGSSQFFDDENFST 248
IV IL APR S + K W+ GSQG L+ GFQ LN+AR WIGSS F ++N +
Sbjct: 187 QIVPILNAPRTSFTES-KGKCNSWTSGSQGFLVVGFQNLNNARLVWIGSSDFLKNKNQDS 245
Query: 249 NGPFIEELTKWTFREKSVIKSTGAVHNHASGVSFEEVPYKIKDEIVYEIGLSVWDGAKWS 308
N F +EL KWTF EKSVIKS AVH+HA G S++E PYKIKD+++Y +G S W+G +W
Sbjct: 246 NQEFAKELLKWTFNEKSVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGEEWL 305
Query: 309 PFTTNDVQFELRLVDPYYRLTLNASRRTEDTQYYSTGNFKLPDHHGMFTFLTDYKRSGLS 368
P +D+QFELR VDPYYRLTL+ S +TQYY+TG F LPD HG+FTFLTDY++ GLS
Sbjct: 306 PHIADDIQFELRQVDPYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLS 365
Query: 369 FITESDTKAIRHLANDEYARSYEITNSWVYLTAIGSVIISFILFVAFFIASSS 421
F T+ D KAIRHLANDEY RS+EI+NSWVY++AI VI+++I FV F+ +SS
Sbjct: 366 FTTDKDVKAIRHLANDEYPRSWEISNSWVYISAICGVIVAWIFFVVSFVTTSS 418
>SPCC338.15 [O] KOG2754 Oligosaccharyltransferase beta subunit
Length = 437
Score = 158 bits (399), Expect = 2e-38
Identities = 113/412 (27%), Positives = 197/412 (47%), Gaps = 25/412 (6%)
Query: 39 DLDNYSKFLDSLKQRSYDIQLASVNEDAEVLSLYNNDERLYDNLIIFPLKTRQISKQIST 98
D+ YS FL SL R +D++ + + +++ L Y ERLYDNLI+ +++ + S
Sbjct: 31 DIGGYSTFLQSLTDRDFDVKTSYIKDESAKLFEYG--ERLYDNLILLSSQSKSLGPVFSP 88
Query: 99 SKLVQFHTEGGDIMAITSPEGLVDPVRLFLNELGIYPSPKNFLLKNFNENGPSGSHEIST 158
L++F GG++ + + L + +R +L ++ + + ++ + + N GS +I
Sbjct: 89 KSLLEFVQSGGNLFVVAGSQ-LPEGIRELGRQLDMFLAERQSVVVD-HFNHAEGSDDIIL 146
Query: 159 SNILNRYVYDGSREFE----LNYEGSAALLDNGEYIVEILRAPRESVCKDINNKEE---- 210
+ ++ Y S E + Y+G L ILR S + + E
Sbjct: 147 LDGISENPYIISDETRAAGPILYKGIGHYLGPNPQTQPILRGNPTSYIYNTKTEAEVSKN 206
Query: 211 DWSVGSQGHLIAGFQTLNSARTTWIGSSQFFDDENFSTNGP--------FIEELTKWTFR 262
W+ G+Q L++ Q+ R GS DE S P F +LT W F+
Sbjct: 207 PWAAGTQLFLVSVLQSSTGERVGLSGSIDMLKDEYLSPQSPSFSKSNFLFARDLTNWVFQ 266
Query: 263 EKSVIKSTGAVHNHASGV--SFEEVPYKIKDEIVYEIGLSVWDGAKWSPFTTNDVQFELR 320
K V+++T + + S Y+IKDE+++ I +S+ + + +P+ +DVQ EL
Sbjct: 267 RKGVLQATNMTYGKVAEPLESRNASCYRIKDEMIFSIDISLLEDGQQTPYVADDVQLELI 326
Query: 321 LVDPYYRLTLNASRRTEDTQYYSTGNFKLPDHHGMFTFLTDYKRSGLSFITESDTKAIRH 380
++DPYYR+ L T + PDH+G FTF +YKR GL+ I E T +R
Sbjct: 327 MLDPYYRVNLVPVPSDSQTSQHYEAVLVAPDHYGDFTFKIEYKRPGLTPIEEKSTFTLRQ 386
Query: 381 LANDEYARSYEITNSWVYLTAIGSVIISFILFVAFFIASSSSLP-VDSVKKQ 431
++E+ R + +++ Y + SV+ +F+LF ++ + P V S KKQ
Sbjct: 387 FFHNEFPRF--LPHAYPYYASCFSVLGAFLLFCGIWLLQKPAKPVVPSAKKQ 436
>Hs20070197 [O] KOG2754 Oligosaccharyltransferase beta subunit
Length = 456
Score = 144 bits (363), Expect = 3e-34
Identities = 118/433 (27%), Positives = 194/433 (44%), Gaps = 38/433 (8%)
Query: 10 AIFFLALGAVNALSVHGLKTLVIYDNRVTDLDNYSKFLDSLKQRSYDIQLASVNEDAEVL 69
A+F+L L + A+ G +TLV+ DN + + +S F SLK R +++ + ++ + L
Sbjct: 28 ALFWLLLPLLGAVCASGPRTLVLLDN-LNVRETHSLFFRSLKDRGFELTFKTADDPS--L 84
Query: 70 SLYNNDERLYDNLIIFPLKTRQISKQISTSKLVQFHTEGGDIMAITSPEGLVDPVRLFLN 129
SL E LYDNLIIF I+ + F GG ++ S + + DP+R +
Sbjct: 85 SLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSD-IGDPLRELGS 143
Query: 130 ELGI-YPSPKNFLLKNFNEN-GPSGSHEI---STSNILNRYVYDGSREFE-LNYEGSAAL 183
E GI + K ++ + N + G H + T N+L G + + G +
Sbjct: 144 ECGIEFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMV 203
Query: 184 LD-NGEYIVEILRAPRESVC--KDINNKEEDWSVGSQGHLIAGFQTLNSARTTWIGSSQF 240
D + +++IL S D + +VG LIAG Q N+AR + GS F
Sbjct: 204 ADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDF 263
Query: 241 FDDENFST----------------NGPFIEELTKWTFREKSVIKSTGAVHNHASGVSFEE 284
F D F++ N L++W F+E+ V++ G V +H G +
Sbjct: 264 FSDSFFNSAVQKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLR-VGPVSHHRVGETAPP 322
Query: 285 VPYKIKDEIVYEIGLSVWDGAKWSPFTTNDVQFELRLVDPYYRLTLNASRRTEDTQYYST 344
Y + D + Y I + KW PF +D+Q E +DP+ R L Q
Sbjct: 323 NAYTVTDLVEYSIVIQQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQ---- 378
Query: 345 GNFKLPDHHGMFTFLTDYKRSGLSFITESDTKAIRHLANDEYARSYEITNSWVYLTAIGS 404
FKLPD +G+F F DY R G + + S ++R L + +Y R I +++ Y + S
Sbjct: 379 --FKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERF--IPSAYPYYASAFS 434
Query: 405 VIISFILFVAFFI 417
+++ +F F+
Sbjct: 435 MMLGLFIFSIVFL 447
>7291015 [O] KOG2754 Oligosaccharyltransferase beta subunit
Length = 449
Score = 135 bits (340), Expect = 1e-31
Identities = 112/435 (25%), Positives = 194/435 (43%), Gaps = 39/435 (8%)
Query: 11 IFFLALGAVNALSVHGLKTLVIYDNRVTDLDNYSKFLDSLKQRSYDIQLASVNEDAEVLS 70
I LA+ A+ TLV+ DN + + +S F SL+ R + + ++ + +LS
Sbjct: 8 IAVLAIAHCQAVLETDANTLVLLDN-LAIRETHSIFFKSLQDRGFKLTYKLADDSSLLLS 66
Query: 71 LYNNDERLYDNLIIFPLKTRQISKQISTSKLVQFHTEGGDIMAITSPEGLVDPVRLFLNE 130
Y E LY N+IIF + +S +L QF +GG+++ + E D +R F +E
Sbjct: 67 KYG--EYLYKNVIIFAPSVEEFGGDVSVERLAQFVDDGGNVL-VAGSEKSGDALREFASE 123
Query: 131 LGIYPSPKN-----FLLKNFNENGPSGSHEISTSNILNRYVYDGSREFE-----LNYEGS 180
G +N L + ++ G + S N++ G + L Y G+
Sbjct: 124 CGFELDEENAAVIDHLHYDVSDAGEHTTILTSAKNLIQADTIVGKANRQADAAPLLYRGT 183
Query: 181 AALLDN-GEYIVEILRAPRESVCKDINNKEEDW--SVGSQGHLIAGFQTLNSARTTWIGS 237
+ D ++++L A + + D+ +VG LIA Q N+AR + GS
Sbjct: 184 GLIADKENPLVLKLLTAESTAYSYNPEASVSDYPHAVGRGTLLIAALQARNNARVVFSGS 243
Query: 238 SQFFDDENFST---------------NGPFIEELTKWTFREKSVIKSTGAVHNHASGVSF 282
FF DE+F+T N E ++KW F E ++ H+ +
Sbjct: 244 LLFFSDESFTTAVQYAQSGVFHKLAGNRDVAESISKWVFGETGRLRVASVQHHKEGELLP 303
Query: 283 EEVPYKIKDEIVYEIGLSVWDGAKWSPFTTNDVQFELRLVDPYYRLTLNASRRTEDTQYY 342
+ Y I D +VY IG+ +W F +D+Q E +DP+ R L ++T Y
Sbjct: 304 PDQAYTITDPVVYTIGIEELVQGEWRAFKASDIQLEFVRIDPFVRTYL---KQTNTGAYQ 360
Query: 343 STGNFKLPDHHGMFTFLTDYKRSGLSFITESDTKAIRHLANDEYARSYEITNSWVYLTAI 402
+ FK+PD +G++ F DY R G + + + ++R L + +Y R I +++ Y T+
Sbjct: 361 A--KFKIPDVYGVYQFKVDYNRVGYTHLYSTTQVSVRPLEHTQYERF--IPSAFPYYTSA 416
Query: 403 GSVIISFILFVAFFI 417
S++I +F F+
Sbjct: 417 FSMMIGVFVFSFVFL 431
>At5g66680 [O] KOG2754 Oligosaccharyltransferase beta subunit
Length = 442
Score = 122 bits (307), Expect = 8e-28
Identities = 105/415 (25%), Positives = 195/415 (46%), Gaps = 42/415 (10%)
Query: 28 KTLVIYDNRVTDLDNYSKFLDSLKQRSYDIQLASVNEDAEVLSLYNNDERLYDNLIIFPL 87
+ LV+ D+ ++ ++S F ++LK R +D+ + ED++ L+L + LYD LIIF
Sbjct: 32 RVLVLLDD-LSLKSSHSIFFNTLKSRGFDLDF-KLAEDSK-LALQRYGQYLYDGLIIFAP 88
Query: 88 KTRQISKQISTSKLVQFHTEGGDIMAITSPEGLVDPVRLFLNELGI-YPSPKNFLL---K 143
T + + + + F G D++ +++ D +R E G+ + + ++
Sbjct: 89 STERFGGSLDSKSIADFVDSGRDLI-LSADTAASDLIRGIATECGVDFDEDSSAMVIDHT 147
Query: 144 NFNENGPSGSHE-ISTSNILNRYVYDGSREFE--LNYEGSAALLD-NGEYIVEILRAPRE 199
+F+ + G H I+ +++ V G + E + + G A L+ ++++L A
Sbjct: 148 SFSVSDVDGDHTLIAADDLVKSDVILGKTKIEAPVLFRGVAHSLNPTNNLVLKVLSASPS 207
Query: 200 SVCKDINNK--EEDWSVGSQGHLIAGFQTL-----NSARTTWIGSSQFFDDENF------ 246
+ + ++K GS L++ Q N+AR GS Q F D
Sbjct: 208 AYSANPSSKLSSPPQLTGSSISLVSVMQVCLSSARNNARVVISGSVQLFSDRLIRSGVQK 267
Query: 247 ---------STNGPFIEELTKWTFREKSVIKSTGAVHNHASGVSFEEVPYKIKDEIVYEI 297
S N F+ EL+KW F E+ +K+ VH H G + E Y+IKD++ + +
Sbjct: 268 AGSPNQYEKSGNEQFVTELSKWVFHERGHLKAGNLVH-HRVGETDEPAIYRIKDDLEFSV 326
Query: 298 GLSVWDGAKWSPFTTNDVQFELRLVDPYYRLTLNASRRTEDTQYYSTGNFKLPDHHGMFT 357
+ W G W P+ NDVQ + ++ PY TL+ T+ + T +FK+PD +G+F
Sbjct: 327 EIYEWSGKSWEPYVANDVQVQFYMMSPYVLKTLS----TDKKGLFHT-SFKVPDVYGVFQ 381
Query: 358 FLTDYKRSGLSFITESDTKAIRHLANDEYARSYEITNSWVYLTAIGSVIISFILF 412
F +Y++ G + ++ S +R ++EY R I ++ Y A + + F +F
Sbjct: 382 FKVEYEKLGYTTLSLSKQIPVRPYRHNEYERF--IPTAYPYYGACFTTMAGFFVF 434
>CE01081 [O] KOG2754 Oligosaccharyltransferase beta subunit
Length = 445
Score = 115 bits (288), Expect = 1e-25
Identities = 99/416 (23%), Positives = 188/416 (44%), Gaps = 40/416 (9%)
Query: 41 DNYSKFLDSLKQRSYDIQLASVNEDAEVLSLYNNDERLYDNLIIFPLKTRQISKQISTSK 100
D +S FL+S+K+R +++ + + ++ L+L+ + + ++D+L I + +S S+
Sbjct: 34 DTHSVFLNSVKERGHELTVRAADDSQ--LALFKHGQLIFDHLFILAPGVQVFGGSLSPSE 91
Query: 101 LVQFHTEGGDIMAITSPEGLVDPVRLFLNELGIYPSPKNFLL---KNFNENGPSGSHE-- 155
+ +F GG+++ + + + D +R E G + N+++ SG H
Sbjct: 92 ISKFVDAGGNVL-VAAGSNIGDALREIAAEHGFEFEEAGTSVIDHHNYDQTLDSGDHTTL 150
Query: 156 -ISTSNILNRYVYDGS--REFELNYEGSAALLDNGEYI-VEILRAPRESVCKDIN--NKE 209
+ +++ + G+ + + ++G + + + I+RA + D
Sbjct: 151 VVGKDQLISAELIVGNSAKLHPVLFKGIGLVAGKTNNLALSIVRASGTAYSYDPKAVRAT 210
Query: 210 EDWSVGSQGHLIAGFQTLNSARTTWIGSSQFFDDENFST---------------NGPFIE 254
GS+ L+ G Q+ N+AR + GSS+ F + FS N F
Sbjct: 211 NPSIAGSRTLLVGGLQSRNNARIVFTGSSELFSNTFFSAKTNSVNPSVQGAQSGNADFAT 270
Query: 255 ELTKWTFREKSVIKSTGAVHNHASGVSFEEVP-YKIKDEIVYEIGLSVWDGAKWSPFTTN 313
+T+W +E V++ V++H G + V Y I +++VY I + KW PF
Sbjct: 271 AITRWVMKESGVLR-VKTVNHHKKGETVPPVEGYFITEDVVYTIEIEELKNGKWVPFQGK 329
Query: 314 DVQFELRLVDPYYRLTLNASRRTEDTQYYSTGNFKLPDHHGMFTFLTDYKRSGLSFITES 373
DVQ E +DP+ R TL S FKLPD G+F FL DY+R G + + +
Sbjct: 330 DVQLEFVRIDPFVRATLKNSNGRLSVA------FKLPDVLGVFKFLVDYRRVGYTHLYDV 383
Query: 374 DTKAIRHLANDEYARSYEITNSWVYLTAIGSVIISFILFVAFFIASSSSLPVDSVK 429
++R L + +Y R I +++ Y + S++ +LF ++ + PV K
Sbjct: 384 QQVSVRPLWHTQYERF--IRSAYPYYASSFSMMAGLVLFSIVYLYHKDT-PVKGAK 436
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,859,504
Number of Sequences: 60738
Number of extensions: 1147870
Number of successful extensions: 2774
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2747
Number of HSP's gapped (non-prelim): 7
length of query: 432
length of database: 30,389,216
effective HSP length: 109
effective length of query: 323
effective length of database: 23,768,774
effective search space: 7677314002
effective search space used: 7677314002
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)