ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3580 good E KOG2367 Amino acid transport and metabolism Alpha-isopropylmalate synthase/homocitrate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3580 1213327 1215075 583
(583 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL104c [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate s... 694 0.0
YOR108w [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate s... 672 0.0
SPBC3E7.16c [E] KOG2367 Alpha-isopropylmalate synthase/homocitra... 485 e-137
At5g23010 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 105 1e-22
At1g18500 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 101 3e-21
At1g74040 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 101 4e-21
At5g23020 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate... 97 5e-20
>YNL104c [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 619
Score = 694 bits (1791), Expect = 0.0
Identities = 353/585 (60%), Positives = 433/585 (73%), Gaps = 18/585 (3%)
Query: 5 KDPSVKYKPFVSNVKLQDRKWPSKTLNKAPRWLATDLRDGNQSLPDPMNLEEKKLMLDKL 64
KDPS KYKPF + KL +RKWP + +APRWL+TDLRDGNQSLPDPM++E+KK KL
Sbjct: 33 KDPSSKYKPF-NAPKLSNRKWPDNRITRAPRWLSTDLRDGNQSLPDPMSVEQKKEYFHKL 91
Query: 65 CELGFKEIEVAFPSASNIDFQFTQYAVKNVPEDVSIQVLSPCREPLIERTVESLVGAKRA 124
+GFKEIEV+FPSAS DF FT+YAV+N P+DVSIQ L RE LI+RTVE+L GAK+A
Sbjct: 92 VNIGFKEIEVSFPSASQTDFDFTRYAVENAPDDVSIQCLVQSREHLIKRTVEALTGAKKA 151
Query: 125 IVHIYLATSPCFREIVFNNMSHEESIEKAVKCAKLVRSLTKDHPDRQDTHWSFEFSPETF 184
+H YLATS FREIVFN MS EE+I KAV+ KLVR LTKD P +Q T WS+EFSPE F
Sbjct: 152 TIHTYLATSDMFREIVFN-MSREEAISKAVEATKLVRKLTKDDPSQQATRWSYEFSPECF 210
Query: 185 SDSEPDFVLEICEAVKAAWGPTEDNPIIFNLPATVEMATPNVYADQIEYFAQSISEREKV 244
SD+ +F +EICEAVK AW PTE+NPIIFNLPATVE+A+PNVYADQIEYFA I+EREKV
Sbjct: 211 SDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATVEVASPNVYADQIEYFATHITEREKV 270
Query: 245 CISLHPHNDRGCAVAAAELAQMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVSPNL 304
CIS H HNDRGC VAA EL +AGADRVEGCLFGNGERTGNVDLVT+A+N+YTQGVSPNL
Sbjct: 271 CISTHCHNDRGCGVAATELGMLAGADRVEGCLFGNGERTGNVDLVTVAMNMYTQGVSPNL 330
Query: 305 DFSDMASIIEVVEKCNKIPVHARAPYGGQLVVCAFSGSHQDAIKKGFEKYDNKVKALQEK 364
DFSD+ S+++VVE+CNKIPV RAPYGG LVVCAFSGSHQDAIKKGF LQ K
Sbjct: 331 DFSDLTSVLDVVERCNKIPVSQRAPYGGDLVVCAFSGSHQDAIKKGFN--------LQNK 382
Query: 365 EGPDAVVPWKMPYLPLDPQDIGRTYEAIIRVNSQSGKGGSSWVILKNLELDLPRDLQIAY 424
+ W++PYLPLDP+DIGR YEA+IRVNSQSGKGG++WVIL++L LDLPR++QI +
Sbjct: 383 KRAQGETQWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVILRSLGLDLPRNMQIEF 442
Query: 425 SKIVQNETEIVGRELKSDELISLFEKSYFVGSHSTTGKFKFIDYKYDK-SPENFTLSVQL 483
S VQ+ + +GRELKSDE+ LF+++Y ++Y +K E + Q+
Sbjct: 443 SSAVQDHADSLGRELKSDEISKLFKEAYNYNDEQYQA-ISLVNYNVEKFGTERRVFTGQV 501
Query: 484 SDGTTQWDLEGTGNGPISSFIDAVNKNFKTNLDVKNYHEHSLGKSSDSRAATYISVSH-- 541
G D+EGTGNGPISS +DA++ V NY EHSLG S ++AA+YI +S+
Sbjct: 502 KVGDQIVDIEGTGNGPISSLVDALSNLLNVRFAVANYTEHSLGSGSSTQAASYIHLSYRR 561
Query: 542 ----EGFVQWGVGIHEDTTLASFLALLSCINGLDRAKNFTVNSAA 582
E +WGVG+ ED +S A+ + IN + + + ++ S A
Sbjct: 562 NADNEKAYKWGVGVSEDVGDSSVRAIFATINNIIHSGDVSIPSLA 606
>YOR108w [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 604
Score = 672 bits (1734), Expect = 0.0
Identities = 341/574 (59%), Positives = 425/574 (73%), Gaps = 19/574 (3%)
Query: 5 KDPSVKYKPFVSNVKLQDRKWPSKTLNKAPRWLATDLRDGNQSLPDPMNLEEKKLMLDKL 64
+DPSVKY+ F K+ R WP KT+ KAPRWL+TDLRDGNQSLPDPM++ +KK KL
Sbjct: 32 RDPSVKYRAFAPP-KMVKRIWPDKTIQKAPRWLSTDLRDGNQSLPDPMSVAQKKEYFHKL 90
Query: 65 CELGFKEIEVAFPSASNIDFQFTQYAVKNVPEDVSIQVLSPCREPLIERTVESLVGAKRA 124
+GFKEIEV+FPSAS DF FT+YAV+N P+DV IQ L RE LI+RTVE+L GAKRA
Sbjct: 91 INIGFKEIEVSFPSASQTDFDFTRYAVENAPDDVGIQCLVQSREHLIKRTVEALTGAKRA 150
Query: 125 IVHIYLATSPCFREIVFNNMSHEESIEKAVKCAKLVRSLTKDHPDRQDTHWSFEFSPETF 184
+H YLATS FREIVFN MS EE+I KAV+ KLVR LTKD P +Q T WS+EFSPE F
Sbjct: 151 TIHTYLATSDMFREIVFN-MSREEAISKAVEATKLVRKLTKDDPSQQATRWSYEFSPECF 209
Query: 185 SDSEPDFVLEICEAVKAAWGPTEDNPIIFNLPATVEMATPNVYADQIEYFAQSISEREKV 244
SD+ +F +EICEAVK AW PTE+NPIIFNLPATVE+A+PNVYADQIEYF+ I+EREKV
Sbjct: 210 SDTPGEFAVEICEAVKKAWEPTEENPIIFNLPATVEVASPNVYADQIEYFSTHITEREKV 269
Query: 245 CISLHPHNDRGCAVAAAELAQMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVSPNL 304
CIS H HNDRGC VAA EL +AGADRVEGCLFGNGERTGNVDLVT+A+N+YTQGVSPNL
Sbjct: 270 CISTHCHNDRGCGVAATELGMLAGADRVEGCLFGNGERTGNVDLVTVAMNMYTQGVSPNL 329
Query: 305 DFSDMASIIEVVEKCNKIPVHARAPYGGQLVVCAFSGSHQDAIKKGFEKYDNKVKALQEK 364
DFSD+ SI E+V +CNKIP+ RAPYGG+LVV AFSGSHQDAIKKGF A+Q K
Sbjct: 330 DFSDLTSISEIVHRCNKIPIPPRAPYGGELVVSAFSGSHQDAIKKGF--------AIQNK 381
Query: 365 EGPDAVVPWKMPYLPLDPQDIGRTYEAIIRVNSQSGKGGSSWVILKNLELDLPRDLQIAY 424
+ W++PYLPLDP+DIGR YEA+IRVNSQSGKGG++WVI+++L LD+PR +Q+ +
Sbjct: 382 KQAQGETRWRIPYLPLDPKDIGRDYEAVIRVNSQSGKGGAAWVIMRSLGLDVPRPMQVDF 441
Query: 425 SKIVQNETEIVGRELKSDELISLFEKSYFVGSHSTTGKFKFIDYKYDK-SPENFTLSVQL 483
S +Q + +GRELKS+E+ LF+++Y ++ ++Y+ K +PE L Q+
Sbjct: 442 SNTLQKNADALGRELKSEEITKLFKETYNYNNNEHI-YVTLLNYEVKKLNPERRALVGQV 500
Query: 484 SDGTTQWDLEGTGNGPISSFIDAVNKNFKTNLDVKNYHEHSLGKSSDSRAATYISVS--- 540
++EG GNGPISS +DA++ L V+NY EHSLG S ++AA++I++S
Sbjct: 501 EINDKVVNIEGYGNGPISSLVDALSNLLNVKLSVQNYSEHSLGSGSATQAASFINLSYIK 560
Query: 541 ----HEGFVQWGVGIHEDTTLASFLALLSCINGL 570
H WGVG+ EDT AS A+ + +N +
Sbjct: 561 DINNHATSNMWGVGVSEDTGDASIKAVFATVNNI 594
>SPBC3E7.16c [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 460
Score = 485 bits (1248), Expect = e-137
Identities = 247/470 (52%), Positives = 323/470 (68%), Gaps = 20/470 (4%)
Query: 111 IERTVESLVGAKRAIVHIYLATSPCFREIVFNNMSHEESIEKAVKCAKLVRSLTKDHPDR 170
I RT+E+L GAK A VH+Y A SP FRE+VF N S +E+++ A+K +K+V + TK+ +
Sbjct: 1 ILRTIEALRGAKNATVHLYNACSPLFREVVFRN-SKQETLDLAIKGSKIVTAATKNALES 59
Query: 171 QDTHWSFEFSPETFSDSEPDFVLEICEAVKAAWGPTEDNPIIFNLPATVEMATPNVYADQ 230
++T+W FE+SPETFSD+EPDF LE+CEAVK W P+ PIIFNLPATVEM+TPN YAD
Sbjct: 60 KETNWGFEYSPETFSDTEPDFALEVCEAVKGMWKPSAAQPIIFNLPATVEMSTPNTYADL 119
Query: 231 IEYFAQSISEREKVCISLHPHNDRGCAVAAAELAQMAGADRVEGCLFGNGERTGNVDLVT 290
IEYF+ +ISEREKVC+SLHPHNDRG AVAAAEL Q+AG DR+EGCLFGNGERTGNVDLVT
Sbjct: 120 IEYFSTNISEREKVCVSLHPHNDRGTAVAAAELGQLAGGDRIEGCLFGNGERTGNVDLVT 179
Query: 291 LALNLYTQGVSPNLDFSDMASIIEVVEKCNKIPVHARAPYGGQLVVCAFSGSHQDAIKKG 350
LA NLYTQGVSPNLDFS + II + E CNKI VH R PY G LV AFSGSHQDAI KG
Sbjct: 180 LAFNLYTQGVSPNLDFSKLDEIIRITEDCNKINVHPRHPYAGNLVFTAFSGSHQDAISKG 239
Query: 351 FEKYDNKVKALQEKEGPDAVVPWKMPYLPLDPQDIGRTYEAIIRVNSQSGKGGSSWVILK 410
+ YD E++ D V WK+PYLPLDP D+ Y AIIRVNSQSGKGG ++++
Sbjct: 240 LKAYD-------ERKAVDPV--WKVPYLPLDPHDVNSEYAAIIRVNSQSGKGGVAYLLKT 290
Query: 411 NLELDLPRDLQIAYSKIVQNETEIVGRELKSDELISLFEKSYFVGSHSTTGKFKFIDYKY 470
N LDLPR LQ+ + IV++ ++ G+EL E+ LF +Y++ G+F DY
Sbjct: 291 NCGLDLPRALQVEFGSIVKDYSDTKGKELSIGEISDLFYTTYYL---EFPGRFSVNDYTL 347
Query: 471 DKS-PEN------FTLSVQLSDGTTQWDLEGTGNGPISSFIDAVNKNFKTNLDVKNYHEH 523
+ P++ + + D ++ +EG GNGP+S+ +DA+ + F + D+ Y EH
Sbjct: 348 SSNGPQSKCIKCVVDIKGEKKDTPSRVVIEGVGNGPLSALVDALRRQFNISFDIGQYSEH 407
Query: 524 SLGKSSDSRAATYISVSHEGFVQWGVGIHEDTTLASFLALLSCINGLDRA 573
++G + +AA+Y+ + WGVGI D T A A++S ++ RA
Sbjct: 408 AIGSGNGVKAASYVEIIFNNTSFWGVGIDADVTSAGLKAVMSGVSRASRA 457
>At5g23010 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 506
Score = 105 bits (263), Expect = 1e-22
Identities = 100/352 (28%), Positives = 155/352 (43%), Gaps = 37/352 (10%)
Query: 24 KWPSKTLNKAP-----RWLATDLRDGNQSLPDPMNLEEKKLMLDKLCELGFKEIEVAFPS 78
+WP NK P R T LRDG QS + +K + +L +L +EV FP
Sbjct: 70 RWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQKLEIARQLAKLRVDIMEVGFPG 129
Query: 79 ASNIDFQFTQYAVKNVPEDVS--------IQVLSPCREPLIERTVESLVGAKRAIVHIYL 130
+S + + + K V +V I ++ C+ IE T E+L AKR + ++
Sbjct: 130 SSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKHRDIEATWEALKYAKRPRILVFT 189
Query: 131 ATSPCFREIVFNNMSHEESIEKAVKCAKLVRSLTKDHPDRQDTHWSFEFSPETFSDSEPD 190
+TS + + EE IE AV + +SL + +F E S+ D
Sbjct: 190 STSDIHMKYKLKK-TQEEVIEMAVSSIRFAKSLGFN---------DIQFGCEDGGRSDKD 239
Query: 191 FVLEIC-EAVKAAWGPTEDNPIIFNLPATVEMATPNVYADQIEYFAQSISEREKVCISLH 249
F+ +I EA+KA G T + + TV + P+ Y + + Y + + V +++H
Sbjct: 240 FLCKILGEAIKA--GVT-----VVTIGDTVGINMPHEYGELVTYLKANTPGIDDVVVAVH 292
Query: 250 PHNDRGCAVAAAELAQMAGADRVEGCLFGNGERTGNVDL--VTLALN----LYTQGVSPN 303
HND G A A + AGA +VE + G GER+GN L V +AL GV
Sbjct: 293 CHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVINGVYTK 352
Query: 304 LDFSDMASIIEVVEKCNKIPVHARAPYGGQLVVCAFSGSHQDAIKKGFEKYD 355
+D + + ++V++ + V A P G SG HQD I K Y+
Sbjct: 353 IDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYE 404
>At1g18500 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 660
Score = 101 bits (252), Expect = 3e-21
Identities = 97/334 (29%), Positives = 140/334 (41%), Gaps = 37/334 (11%)
Query: 39 TDLRDGNQSLPDPMNLEEKKLMLDKLCELGFKEIEVAFPSASNIDFQFTQYAVKNVPEDV 98
T LRDG QS + +EK + +L +LG IE FP+AS DF+ AVK + E V
Sbjct: 94 TTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFE----AVKTIAETV 149
Query: 99 SIQV-----------LSPCREPLIERTVESLVGAKRAIVHIYLATSPCFREIVFNNMSHE 147
V LS C + IER +++ AKR +H ++ATS E +
Sbjct: 150 GNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKK-TKA 208
Query: 148 ESIEKAVKCAKLVRSLTKDHPDRQDTHWSFEFSPETFSDSEPDFVLEICEAVKAAWGPTE 207
E IE A + RSL + EFSPE SE +++ EI V A T
Sbjct: 209 EVIEIARSMVRFARSLGCE---------DVEFSPEDAGRSEREYLYEILGEVIKAGATT- 258
Query: 208 DNPIIFNLPATVEMATPNVYADQIEYFAQSISEREKVCISLHPHNDRGCAVAAAELAQMA 267
N+P TV + P+ + I + E V IS H ND G + A A
Sbjct: 259 -----LNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHA 313
Query: 268 GADRVEGCLFGNGERTGNVDLVTLALNLYTQ------GVSPNLDFSDMASIIEVVEKCNK 321
GA ++E + G GER GN L + + + + G+ +D + ++VE+
Sbjct: 314 GARQMEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTG 373
Query: 322 IPVHARAPYGGQLVVCAFSGSHQDAIKKGFEKYD 355
+ G SG HQD + K Y+
Sbjct: 374 MQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYE 407
>At1g74040 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 631
Score = 101 bits (251), Expect = 4e-21
Identities = 97/334 (29%), Positives = 140/334 (41%), Gaps = 37/334 (11%)
Query: 39 TDLRDGNQSLPDPMNLEEKKLMLDKLCELGFKEIEVAFPSASNIDFQFTQYAVKNVPEDV 98
T LRDG QS + +EK + +L +LG IE FP+AS DF+ AVK + E V
Sbjct: 92 TTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFE----AVKTIAETV 147
Query: 99 SIQV-----------LSPCREPLIERTVESLVGAKRAIVHIYLATSPCFREIVFNNMSHE 147
V LS C + IE E++ AKR +H ++ATS + S E
Sbjct: 148 GNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKLKK-SKE 206
Query: 148 ESIEKAVKCAKLVRSLTKDHPDRQDTHWSFEFSPETFSDSEPDFVLEICEAVKAAWGPTE 207
E IE A + RSL + EFSPE SE +++ EI V A T
Sbjct: 207 EVIEIARNMVRFARSLGCE---------DVEFSPEDAGRSEREYLYEILGEVIKAGATT- 256
Query: 208 DNPIIFNLPATVEMATPNVYADQIEYFAQSISEREKVCISLHPHNDRGCAVAAAELAQMA 267
N+P TV + P+ + I + + V IS H ND G + A +
Sbjct: 257 -----LNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHS 311
Query: 268 GADRVEGCLFGNGERTGNVDLVTLALNLYTQ------GVSPNLDFSDMASIIEVVEKCNK 321
GA +VE + G GER GN L + + + + G+ +D + ++VE+
Sbjct: 312 GARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTG 371
Query: 322 IPVHARAPYGGQLVVCAFSGSHQDAIKKGFEKYD 355
+ G SG HQD + K Y+
Sbjct: 372 MQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYE 405
>At5g23020 [E] KOG2367 Alpha-isopropylmalate synthase/homocitrate synthase
Length = 503
Score = 97.4 bits (241), Expect = 5e-20
Identities = 98/351 (27%), Positives = 151/351 (42%), Gaps = 39/351 (11%)
Query: 26 PSKTLNKAP-----RWLATDLRDGNQSLPDPMNLEEKKLMLDKLCELGFKEIEVAFPSAS 80
P NK P R L T LRDG QS + +K + +L +L +EV FP +S
Sbjct: 72 PEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPPQKLEIARQLAKLRVDIMEVGFPVSS 131
Query: 81 NIDFQFTQYAVKNVPEDVSIQV--------LSPCREPLIERTVESLVGAKRAIVHIYLAT 132
+F+ + K V +V + ++ C++ IE T E+L AKR V ++ +T
Sbjct: 132 EEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCKKRDIEATWEALKYAKRPRVMLFTST 191
Query: 133 SPCFREIVFNNMSHEESIEKAVKCAKLVRSLT-KDHPDRQDTHWSFEFSPETFSDSEPDF 191
S + + EE IE AV K +SL KD +F E +E DF
Sbjct: 192 SEIHMKYKLKK-TKEEVIEMAVNSVKYAKSLGFKD----------IQFGCEDGGRTEKDF 240
Query: 192 VLEIC-EAVKAAWGPTEDNPIIFNLPATVEMATPNVYADQIEYFAQSISEREKVCISLHP 250
+ +I E++KA G T TV + P + + + Y ++ + + ++H
Sbjct: 241 ICKILGESIKA--GATT-----VGFADTVGINMPQEFGELVAYVIENTPGADDIVFAIHC 293
Query: 251 HNDRGCAVAAAELAQMAGADRVEGCLFGNGERTGNVDLVTLALNL------YTQGVSPNL 304
HND G A A AGA +VE + G GER+GN L + + L GV +
Sbjct: 294 HNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLMDGVYTKI 353
Query: 305 DFSDMASIIEVVEKCNKIPVHARAPYGGQLVVCAFSGSHQDAIKKGFEKYD 355
D + + ++V++ + V P G SG HQD I K Y+
Sbjct: 354 DSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYE 404
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,641,478
Number of Sequences: 60738
Number of extensions: 1654585
Number of successful extensions: 3911
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3883
Number of HSP's gapped (non-prelim): 7
length of query: 583
length of database: 30,389,216
effective HSP length: 112
effective length of query: 471
effective length of database: 23,586,560
effective search space: 11109269760
effective search space used: 11109269760
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)