ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3656 good I KOG3888 Lipid transport and metabolism Gamma-butyrobetaine,2-oxoglutarate dioxygenase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3656 1234322 1235581 420
(420 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL021c [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase 238 2e-62
7300613 [I] KOG3889 Predicted gamma-butyrobetaine2-oxoglutarate ... 91 2e-18
Hs4502369 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase 91 3e-18
CE03505 [I] KOG3889 Predicted gamma-butyrobetaine2-oxoglutarate ... 90 7e-18
Hs8922625 [I] KOG3889 Predicted gamma-butyrobetaine2-oxoglutarat... 84 4e-16
7292993 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase 78 2e-14
CE00940 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase 76 1e-13
7303323 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase 74 3e-13
7290115_2 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase 72 2e-12
>YHL021c [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 465
Score = 238 bits (606), Expect = 2e-62
Identities = 146/430 (33%), Positives = 218/430 (49%), Gaps = 62/430 (14%)
Query: 35 ITVKFQQQGQVKEQYLDHLLLRDASQSPKSVDPISSQKLFTTGSLLIDRGRPLEPIQPGI 94
IT ++ + +++ LRDAS S K L TTG L + + P
Sbjct: 49 ITFSMEESSKPVSVCFNNVFLRDASHSAK---------LVTTGELYHNE----KLTAPQD 95
Query: 95 ADIVDEGQGLYVHWNDGDQFTYTSEYLQSLVGESV-------SIKKYDPKVWNQEIVSKP 147
I ++G+ L V W DG + ++ G S +Y P++WN+ I+ K
Sbjct: 96 IQISEDGKSLVVKWKDGGHHQFPLQFFIDYKGSSFVSPATRKQESRYRPQLWNKRIL-KD 154
Query: 148 DFLSALSIDYHSYMDPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMICERI 207
+ LS+ Y+ ++DP D+S LF+T+ L ++GI ++ P ++ + +Q ICERI
Sbjct: 155 NVKDLLSVSYNEFIDPKDDSKLFQTLVNLQKFGIAFISGTPSSSSEGLT---IQKICERI 211
Query: 208 GHVRTTFYGE-LFDVKNQASQANNIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEGLE 266
G +R+T +GE FDV + + N Y K LPLH DL +LEN+PG+Q+L + +EG +
Sbjct: 212 GPIRSTVHGEGTFDVNASQATSVNAHYANKDLPLHTDLPFLENVPGFQILQSLPATEGED 271
Query: 267 ENGQ--NYFVDSLGALNYIKNKDPSVLKALETIPITXXXXXXXXXXXXXXPLVEHK---- 320
N + NYFVD+ A ++ D +AL+ +P+ PL+EH
Sbjct: 272 PNTRPMNYFVDAFYATRNVRESDFEAYEALQIVPVNYIYENGDKRYYQSKPLIEHHDINE 331
Query: 321 ------KYETVV---NYSPPFQGPFNL---------------------KDITDIPLLNQF 350
YE ++ NYSPP+Q PF +T+ L F
Sbjct: 332 DNTLLGNYEALIKCINYSPPYQAPFTFGIYDKPSDLNNNLDLNLITTPAKLTERFLFKSF 391
Query: 351 KKGLYMFEEYINDPKNQFQIKLPENSCVIFHNRRILHARR-QFDGERWLKGCYLDADTFS 409
+GL +FE +IND NQF+++LPEN CVIF+NRRILHA ++WLKGCY D+DTF
Sbjct: 392 IRGLNLFESHINDFNNQFRLQLPENCCVIFNNRRILHANSLTSSNQQWLKGCYFDSDTFK 451
Query: 410 SKLSYLYEKF 419
SKL +L EKF
Sbjct: 452 SKLKFLEEKF 461
>7300613 [I] KOG3889 Predicted gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 364
Score = 91.3 bits (225), Expect = 2e-18
Identities = 79/334 (23%), Positives = 136/334 (40%), Gaps = 39/334 (11%)
Query: 96 DIVDEGQGLYVHWNDGDQFTYTSEY-----LQSLVGESVSIKKYDPKVWNQEIVSKPDFL 150
D+ +G L V W+D + Y ++ L+ L+G P WN+ I+ + +
Sbjct: 52 DVKYDGMNLQVQWSDAHKSNYDLDFIFDSQLERLIGRRSKSTNLTP--WNRSIILQNERH 109
Query: 151 SALSIDYHSYMDPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMICERIGHV 210
+ D S ++ L +YGI + D+ A ++ R+ +
Sbjct: 110 LRFPLPQLVSSDNEVRS----LVESLVRYGIVFIDDVAPTAN------MTELALRRVFPL 159
Query: 211 RTTFYGELFDVKNQASQANNIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEGLEENGQ 270
TF+GE++ + A+ AYT L H D Y + G Q LHCI++S G+
Sbjct: 160 MKTFFGEMWTFSDNPDHADT-AYTKLYLGSHTDNTYFCDAAGLQALHCIEHSGS---GGE 215
Query: 271 NYFVDSLGALNYIKNKDPSVLKALETIPITXXXXXXXXXXXXXXPLVEHKKYETVVNYSP 330
N+FVD L ++ +K + P+ L ++ + P+++ P
Sbjct: 216 NFFVDGLHVVHELKRRYPAAYDVLCSVQVPGEYIEKGEHHYHTAPIIQ---------VDP 266
Query: 331 PFQGPFNLK-DITDIPLLN--------QFKKGLYMFEEYINDPKNQFQIKLPENSCVIFH 381
Q L+ ++ D + N +F L + D + Q+ +KL S V+F
Sbjct: 267 LTQEFVQLRLNVYDRAVFNTIPQAEMAEFYDSLRQLLLIVRDKQQQWALKLCPGSIVLFD 326
Query: 382 NRRILHARRQFDGERWLKGCYLDADTFSSKLSYL 415
N R+LH R + G R + G Y+ F SK L
Sbjct: 327 NWRVLHGREAYTGSRTMSGSYVQRTDFLSKARVL 360
>Hs4502369 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 387
Score = 90.9 bits (224), Expect = 3e-18
Identities = 84/346 (24%), Positives = 142/346 (40%), Gaps = 46/346 (13%)
Query: 93 GIADIVDEGQGLYVHWNDGDQFTYTSEYLQSLVGESVSIKKYDPKV-------WNQEI-V 144
GI ++ + + +Y+ W D + +++L+ + K ++ W E+ +
Sbjct: 63 GIKGLIFDRKKVYITWPDEHYSEFQADWLKKRCFSKQARAKLQRELFFPECQYWGSELQL 122
Query: 145 SKPDFLSALSIDYHSYMDPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMIC 204
DF L D H+Y T+KK+ + +D P E V +
Sbjct: 123 PTLDFEDVLRYDEHAY-------KWLSTLKKVGIVRLTGASDKPGE---------VSKLG 166
Query: 205 ERIGHVRTTFYGELFDVKNQASQANNIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEG 264
+R+G + TFYG + V+++ ANN+AYT L H D L + PG QLLHCIK +
Sbjct: 167 KRMGFLYLTFYGHTWQVQDKID-ANNVAYTTGKLSFHTDYPALHHPPGVQLLHCIKQT-- 223
Query: 265 LEENGQNYFVDSLGALNYIKNKDPSVLKALETIPITXXXXXX---XXXXXXXXPLVEHKK 321
G + VD +K +P + L + + ++E
Sbjct: 224 -VTGGDSEIVDGFNVCQKLKKNNPQAFQILSSTFVDFTDIGVDYCDFSVQSKHKIIELDD 282
Query: 322 YETVVNYSPPFQGPFN---LKDITDIPL--LNQFKKGLYMFEEYINDPKNQFQIKLPENS 376
VV + FN I D+P+ + F L F + +N +++F K+
Sbjct: 283 KGQVVRIN------FNNATRDTIFDVPVERVQPFYAALKEFVDLMNSKESKFTFKMNPGD 336
Query: 377 CVIFHNRRILHARRQFDG----ERWLKGCYLDADTFSSKLSYLYEK 418
+ F N R+LH RR ++ R L+G Y D D S+L L ++
Sbjct: 337 VITFDNWRLLHGRRSYEAGTEISRHLEGAYADWDVVMSRLRILRQR 382
>CE03505 [I] KOG3889 Predicted gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 385
Score = 89.7 bits (221), Expect = 7e-18
Identities = 74/332 (22%), Positives = 136/332 (40%), Gaps = 38/332 (11%)
Query: 97 IVDEG-QGLYVHWNDGDQ--FTYTSEYLQSLVGESVSIKKYDPKVWNQEIVSKPDFLSAL 153
I+DE L + W DG Q F + + V ++VS ++WN + + +S
Sbjct: 62 IIDEATNSLQIVWIDGHQSKFKIGNIIREGKVEKNVSNDNKIYELWNSKSLKDVPRISKS 121
Query: 154 SIDYHSYMDPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMICERIGHVRTT 213
++ S+ K L +YG+ +V + + + +C+ + V T
Sbjct: 122 TLSLQSFS------------KNLVKYGVIIVDGVEGTSEAT------EKLCQSLVPVHDT 163
Query: 214 FYGELFDVKNQASQAN----NIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEGLEENG 269
F+G+ + N A+ + AY + + H D Y + PG Q+ HC+ ++ G
Sbjct: 164 FFGQFWVFSNSATNDEPAYEDTAYGSDEIGPHTDGTYFDQTPGIQVFHCLTPAK---TGG 220
Query: 270 QNYFVDSLGALNYIKNKDPSVLKALETIPITXXXX------XXXXXXXXXXPLVEHKKYE 323
VDS ++N+ P + L I+ P++E +
Sbjct: 221 DTVLVDSFYCAEKLRNESPEDFEILCNTKISHHYLEGSPPGSSIHSVSLEKPVIERNSFG 280
Query: 324 TV--VNYSPPFQGPFNLKDITDIPLLN--QFKKGLYMFEEYINDPKNQFQIKLPENSCVI 379
+ + ++P + PF+ + ++ +F + F + ++P N +I L S +
Sbjct: 281 NITQIRFNPYDRAPFSCLNSSEASAAETIKFYEAYEKFSKICHNPDNSIEISLRPGSVIF 340
Query: 380 FHNRRILHARRQFDGERWLKGCYLDADTFSSK 411
N RILH+R F G R + GCYL D F +K
Sbjct: 341 IDNFRILHSRTSFQGYRQMCGCYLSRDNFMAK 372
>Hs8922625 [I] KOG3889 Predicted gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 421
Score = 84.0 bits (206), Expect = 4e-16
Identities = 85/373 (22%), Positives = 146/373 (38%), Gaps = 53/373 (14%)
Query: 51 DHLLLRDASQSPKSVDPISSQKLFTTGSLLIDRGRPLEPIQPGIADIVDEGQGLYVHWND 110
D++ LRD +S + + Q+ T S+ + I+P + + L+ W D
Sbjct: 72 DYVWLRDHCRSASCYNSKTHQRSLDTASVDLC-------IKPKTIRL--DETTLFFTWPD 122
Query: 111 GDQFTYTSEYLQSLVGESVSIKKYDPKV-WNQEIVSKPDFLSALSIDYHSYMDPHDESSL 169
G Y +L E K P++ WN EI + S+D S+++ ++
Sbjct: 123 GHVTKYDLNWLVKNSYEGQKQKVIQPRILWNAEIYQQAQ---VPSVDCQSFLETNEGLKK 179
Query: 170 FETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMICERIGHVRTTFYGELFDVKNQASQAN 229
F ++ YGI V ++P ++ + + ERI +R T YG ++ + S+ +
Sbjct: 180 F--LQNFLLYGIAFVENVPP------TQEHTEKLAERISLIRETIYGRMWYFTSDFSRGD 231
Query: 230 NIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEGLEENGQNYFVDSLGALNYIKNKDPS 289
AYT L H D Y + G Q+ HC+K+ EG G+ VD A + K P
Sbjct: 232 T-AYTKLALDRHTDTTYFQEPCGIQVFHCLKH-EG--TGGRTLLVDGFYAAEQVLQKAPE 287
Query: 290 VLKALETIPITXXXXXXXXX----------------XXXXXPLVEHKKYETVVNYSPPFQ 333
+ L +P+ L+ + Y+ V + P+
Sbjct: 288 EFELLSKVPLKHEYIEDVGECHNHMIGIGPVLNIYPWNKELYLIRYNNYDRAVINTVPYD 347
Query: 334 GPFNLKDITDIPLLNQFKKGLYMFEEYINDPKNQFQIKLPENSCVIFHNRRILHARRQFD 393
+++++ + P+N+F +KL + N R+LH R F
Sbjct: 348 ------------VVHRWYTAHRTLTIELRRPENEFWVKLKPGRVLFIDNWRVLHGRECFT 395
Query: 394 GERWLKGCYLDAD 406
G R L GCYL D
Sbjct: 396 GYRQLCGCYLTRD 408
>7292993 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 416
Score = 78.2 bits (191), Expect = 2e-14
Identities = 82/346 (23%), Positives = 137/346 (38%), Gaps = 43/346 (12%)
Query: 90 IQPGIADIVDEGQGLYVHWNDGDQFTYTSEYLQSLVGESVSIKKY-----DPKVWNQEIV 144
I+P ++ + + V W+D + ++ ++L+ E ++Y P +
Sbjct: 82 IRPVSLQTDEQSKEVLVKWSDAHESRFSLKWLKERCFEPDKQQEYLRDFYRPTTRHWSGA 141
Query: 145 SKPDFLSALSIDYHSYMDPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMIC 204
D S D D S L + ++ L YG+ L+ P E V+ +
Sbjct: 142 EFQDIAQHFSYDEVMSQD----SVLMQWLQALAIYGVALLRGAP------LDEGVVRRLA 191
Query: 205 ERIGHVRTTFYGELFDVKNQASQANNIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEG 264
+R+G +R T YGE F V+ + A N AY + LPLH DL Y E P +LHC+ ++
Sbjct: 192 DRVGFIRRTTYGEEFVVQAKPG-AQNFAYLSLTLPLHTDLPYYEYKPSVNILHCVVQTD- 249
Query: 265 LEENGQNYFVDSLGALNYIKNKDPSVLKALETIPI----TXXXXXXXXXXXXXXPLV--- 317
G N VD + ++ P + L I + P++
Sbjct: 250 -SPGGSNMLVDGFHVADLLRRDHPEDFERLSRIVVDWNDIGSEDGREFHNIWRAPVICLD 308
Query: 318 EHKKYETVVNYSPPFQGPFNLKDITDIPLLNQFKKGLYMFEEYINDPK----NQFQIKLP 373
E +Y + + P FN +PL ++ L +E Y + + K
Sbjct: 309 EEGRYTRINHSVPQRDSHFN------VPL----EEVLPWYESYALFVRLAIADSHAFKTR 358
Query: 374 ENSCVIFHNRRILHARRQFD----GERWLKGCYLDADTFSSKLSYL 415
+ F+N R+LH R +D R++ G YLD D S+L L
Sbjct: 359 PGDVLTFNNIRLLHGRTGYDDSEESPRYIVGAYLDWDIIYSRLRVL 404
>CE00940 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 421
Score = 75.9 bits (185), Expect = 1e-13
Identities = 61/247 (24%), Positives = 106/247 (42%), Gaps = 40/247 (16%)
Query: 200 VQMICERIGHVRTTFYGELFDVKNQASQANNIAYTAK-PLPLHMDLLYLENIPGWQLLHC 258
V+ I +RIG ++ T +G +F+V +A A+N+AY + LP H D L + P Q+LH
Sbjct: 181 VEAIGDRIGMIKRTHFGLVFEVSLKA-DASNMAYASNGGLPFHTDFPSLSHPPQLQMLHM 239
Query: 259 IKNSEGLEENGQNYFVDSLGALNYIKNKDPSVLKALETIPITXXXXXXXXXXXXXXPLVE 318
++++ EE G + FVD ++ + P + K L T +
Sbjct: 240 LQSA---EEGGHSLFVDGFHVAEQLRVEKPEIFKILTTQSMEYIEEGYDV---------- 286
Query: 319 HKKYETVVNYSPPFQGPFNLKDITDIPLLNQFKKGLYM---------------------F 357
H+ + + + + D +N+ + G M F
Sbjct: 287 HEINGKTIRFDYDMCARHKVIRLNDDGKVNKIQFGNAMRSWFYDCEPSKVQDVYRAMKTF 346
Query: 358 EEYINDPKNQFQIKLPENSCVIFHNRRILHARRQF----DGERWLKGCYLDADTFSSKLS 413
EY P+N + +L + V++ N+R+LH R F + R L GCY D D S++
Sbjct: 347 TEYCYQPRNMLKFRLEDGDTVLWANQRLLHTRDGFRNAPEKARTLTGCYFDWDIVKSRVR 406
Query: 414 YLYEKFN 420
+L +K +
Sbjct: 407 FLRDKLS 413
>7303323 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 402
Score = 74.3 bits (181), Expect = 3e-13
Identities = 53/198 (26%), Positives = 92/198 (45%), Gaps = 12/198 (6%)
Query: 104 LYVHWNDGDQFTYTSEYLQSLVGESVSIKKYDPKVWN--QEIVSKPDFLSALSIDYHSYM 161
L + W+DG Y +L+ + ++Y + Q++ S DF + Y+ +
Sbjct: 88 LCIEWSDGHTSQYPFSWLRERDFSYANRQRYLRDFYRPEQKLWSGLDFEKIRQVFYYQEL 147
Query: 162 DPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMICERIGHVRTTFYGELFDV 221
D S+L + ++ L YG+ +V + P + ++ + R+G +R T YGE F V
Sbjct: 148 LDCD-SALQQWLQHLAVYGVAMVKEAPLDMDV------LRRLSNRVGFMRRTTYGEEFSV 200
Query: 222 KNQASQANNIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEGLEENGQNYFVDSLGALN 281
+ Q +N AY A PLPLH D+ Y E +PG +LH ++ S G N D+
Sbjct: 201 RAQPG-GSNYAYLAAPLPLHTDMPYFEYLPGVTMLHTLEQS--ASPGGVNLLADAFYVAE 257
Query: 282 YIKNKDPSVLKALETIPI 299
++ + P + L P+
Sbjct: 258 VMRERYPEQFRVLCQTPV 275
>7290115_2 [I] KOG3888 Gamma-butyrobetaine2-oxoglutarate dioxygenase
Length = 368
Score = 72.0 bits (175), Expect = 2e-12
Identities = 70/332 (21%), Positives = 135/332 (40%), Gaps = 33/332 (9%)
Query: 100 EGQGLYVHWNDGDQFTYTSEYLQSLVGESVSIKKYDPKVWNQ--EIVSKPDFLSAL-SID 156
E + L + W D + T+ +L+ K Y +V+ ++ K +F
Sbjct: 47 ERKQLEIIWQDKHRSTFDLGWLRERDFGETGRKHYLEEVYKPPAQLWGKTEFEDVKREFQ 106
Query: 157 YHSYMDPHDESSLFETIKKLDQYGIGLVTDIPDEAATNYSEWFVQMICERIGHVRTTFYG 216
Y ++ +++L ++ L G ++ P++ + + ERIG+++ T YG
Sbjct: 107 YEDVLE--QDAALRVWLEALAVQGFAILKGAPNDINV------AKKLAERIGYIKRTTYG 158
Query: 217 ELFDVKNQASQANNIAYTAKPLPLHMDLLYLENIPGWQLLHCIKNSEGLEENGQNYFVDS 276
++F+VK++ + A N AY PLPLH D+ Y E G +LH + SE + G N D
Sbjct: 159 DVFEVKSKPN-ARNYAYLMTPLPLHTDMPYYEYKAGINILHTLVQSE--SKGGANTLTDG 215
Query: 277 LGALNYIKNKDPSVLKALETIPIT------XXXXXXXXXXXXXXPLV---EHKKYETVVN 327
+ ++ P + L+++P+ P++ ++ +
Sbjct: 216 FNVASQLQKLHPEDFEVLKSVPVNWFDIGHDGDDSKPFHSLWRAPVICLDVDGRFARINQ 275
Query: 328 YSPPFQGPFNLKDITDIPLLNQFKKGLYMFEEYINDPKNQFQIKLPENSCVIFHNRRILH 387
+ F++ + + K L + + + K +F+N R+LH
Sbjct: 276 NTTKRDSRFSVSLAQAVSWYKAYDKFLEIAQ------SEAVEFKTQAGDVFVFNNLRMLH 329
Query: 388 ARRQFD----GERWLKGCYLDADTFSSKLSYL 415
R ++ +R L G Y+D D SKL L
Sbjct: 330 GRTAYEDAPGNKRHLVGAYVDWDIIYSKLRTL 361
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.412
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,808,706
Number of Sequences: 60738
Number of extensions: 1139942
Number of successful extensions: 2476
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2453
Number of HSP's gapped (non-prelim): 13
length of query: 420
length of database: 30,389,216
effective HSP length: 109
effective length of query: 311
effective length of database: 23,768,774
effective search space: 7392088714
effective search space used: 7392088714
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)