ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3658 good L KOG2795 Replication, recombination and repair Catalytic subunit of the meiotic double strand break transesterase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3658 1235601 1236803 401
(401 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL022c [L] KOG2795 Catalytic subunit of the meiotic double stra... 135 1e-31
SPAC17A5.11 [L] KOG2795 Catalytic subunit of the meiotic double ... 85 2e-16
Hs6912680 [L] KOG2795 Catalytic subunit of the meiotic double st... 77 5e-14
At3g13170 [L] KOG2795 Catalytic subunit of the meiotic double st... 70 7e-12
7302468 [L] KOG2795 Catalytic subunit of the meiotic double stra... 70 7e-12
At5g02820 [L] KOG2795 Catalytic subunit of the meiotic double st... 66 8e-11
CE06363 [L] KOG2795 Catalytic subunit of the meiotic double stra... 60 7e-09
ECU04g1110 [L] KOG2795 Catalytic subunit of the meiotic double s... 52 2e-06
>YHL022c [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 398
Score = 135 bits (339), Expect = 1e-31
Identities = 102/343 (29%), Positives = 169/343 (48%), Gaps = 42/343 (12%)
Query: 75 ALKEHAIPITVVFNGSITKRKRCEEGSGGKLLRWRYP----EFANTKKKCSKVPQLLQLL 130
+L++H PI++VF K+K+ + S +P + K + LL LL
Sbjct: 59 SLEQHQQPISIVFQN---KKKKGDTSSPDIHTTLDFPLNGPHLCTHQFKLKRCAILLNLL 115
Query: 131 HILNERIKTKKNTTTVRDIYYGNIELFESQQMVEMLFQRIEAMFNVE-KLFFNVVSTQKG 189
++ E++ KNTT VRDI+Y N+ELF+ Q V I F + + N++ QKG
Sbjct: 116 KVVMEKLPLGKNTT-VRDIFYSNVELFQRQANVVQWLDVIRFNFKLSPRKSLNIIPAQKG 174
Query: 190 LIYS---------------GPRLQIGSQLFHAECQLVPYDQGEMIVQ--QNQQLKVIVLE 232
L+YS P++Q +F + L+P+ Q + +++ ++++E
Sbjct: 175 LVYSPFPIDIYDNILTCENEPKMQ-KQTIFPGKPCLIPFFQDDAVIKLGTTSMCNIVIVE 233
Query: 233 KDAIFQQLI-QCQEMIKDWIIVTGKGYPDHLSRELLHKLDLSLQGNV-QFHIFTDSDPYG 290
K+A+F +L+ ++ + +++TGKG+PD L+R L KL+ + IFTD+DPYG
Sbjct: 234 KEAVFTKLVNNYHKLSTNTMLITGKGFPDFLTRLFLKKLEQYCSKLISDCSIFTDADPYG 293
Query: 291 IDIVTKYMVK-------CPRLIHKGVLLNQLITRNTE-SNSMLVLLPLSLRDATFAMGLI 342
I I Y C +KG+ + Q++ +N E N + LL L+ RD + A LI
Sbjct: 294 ISIALNYTHSNERNAYICTMANYKGIRITQVLAQNNEVHNKSIQLLSLNQRDYSLAKNLI 353
Query: 343 RRLCKXXXXXXGSVRKHTMRQNLKYEVQRQLFFQRKGEMNVVN 385
L S K N+ E QR++FFQ+K EMN ++
Sbjct: 354 ASLTANSWDIATSPLK-----NVIIECQREIFFQKKAEMNEID 391
>SPAC17A5.11 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 345
Score = 84.7 bits (208), Expect = 2e-16
Identities = 76/275 (27%), Positives = 130/275 (46%), Gaps = 36/275 (13%)
Query: 123 VPQLLQLLHILNERIKTKKNTTTVRDIYYGNIELFESQQMVEMLFQRIEAMFNVEKLFFN 182
+ QL ++L ++E + T RDIYY +++LF+ Q +V+ L I + N
Sbjct: 71 IAQLFRVLDCVHEAV-ISDTVITKRDIYYRDVDLFKRQTVVDELLGDISNTIGCSRSDLN 129
Query: 183 VVSTQKGLIYSGPR--LQIGSQLFHAECQLVPYDQGEMIVQQNQQLKVIVLEKDAIFQQL 240
V ++ KGL++ L+ G+ + + L+ + + I + V+V+EK+A+FQ L
Sbjct: 130 VEASAKGLVFGSIHIALENGTVITATKPLLISHHRISSITSTAKW--VLVIEKEAVFQTL 187
Query: 241 IQCQEMIKDWIIVTGKGYPDHLSRELLHKLDLSLQGNVQFHIFTDSDPYGIDIVTKYMVK 300
+E + D IIVT KG+PD ++R+ L KL +L F IF D DP+G+ I + +
Sbjct: 188 --TEEALADTIIVTAKGFPDLMTRKFLVKLAKALPDAKFFGIF-DWDPHGLCIYSCFKYG 244
Query: 301 CPRLIHK-----------GVLLNQLITRNTESNSMLVLLPLSLRDATFAMGLIRRLCKXX 349
H+ G L + +N E + L L+ RD L++
Sbjct: 245 SNAYSHEPHSQLRNLQLLGPLYEDIFNKNQEFS-----LKLNKRDIKMITTLLQ------ 293
Query: 350 XXXXGSVRKHTMRQNLKYEVQRQLFFQRKGEMNVV 384
G ++ +R+ L QR LF Q+K E+ +
Sbjct: 294 --FEGFQKEPVVREQL----QRMLFIQKKAEIQAI 322
>Hs6912680 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 396
Score = 77.0 bits (188), Expect = 5e-14
Identities = 86/307 (28%), Positives = 147/307 (47%), Gaps = 39/307 (12%)
Query: 65 ITNNMRFIRLALKEHAIPITVVFNGSITKRKRCEEGSGGKLLRWRYPEFANTKKKCSKVP 124
I N ++ I +L + P + N S + + E+ G +++ T+K S P
Sbjct: 51 IENIIQDIITSLARNEAPAFTIDNRSSWENIKFEDSVGLQMV-----SHCTTRKIKSDSP 105
Query: 125 QLLQ----LLHILNERIKTKKNTT--TVRDIYYGNIELFESQQMVEMLFQRIEAMFNVEK 178
+ Q +L IL+ K ++ T T RDIYY + +LF +Q +V+ + I M V +
Sbjct: 106 KSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLKVSR 165
Query: 179 LFFNVVSTQKGLIYSGPRL--QIGSQL---FHAECQLVPYD-QGEMIVQQNQQLKVIVLE 232
+++ST KGLI R + G+++ A VP + QG + + + V+++E
Sbjct: 166 RSLHILSTSKGLIAGNLRYIEEDGTKVNCTCGATAVAVPSNIQGIRNLVTDAKF-VLIVE 224
Query: 233 KDAIFQQLIQ---CQEMIKDWIIVTGKGYPDHLSRELLHKLDLSLQGNVQFHIFTDSDPY 289
KDA FQ+L+ C + + I++TGKG PD +R L+ KL + +V D+DP+
Sbjct: 225 KDATFQRLLDDNFCNK-LSPCIMITGKGVPDLNTRLLVKKLWDTF--HVPVFTLVDADPH 281
Query: 290 GIDIVTKYM------------VKCPRLIHKGVLLNQLITRNTESNSMLVLLPLSLRDATF 337
GI+I+ Y + P + G+L + L N +S L+PL+ RD
Sbjct: 282 GIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDLKRLNVPKDS---LIPLTKRDQMK 338
Query: 338 AMGLIRR 344
++RR
Sbjct: 339 LDSILRR 345
>At3g13170 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 362
Score = 69.7 bits (169), Expect = 7e-12
Identities = 49/184 (26%), Positives = 92/184 (49%), Gaps = 13/184 (7%)
Query: 122 KVPQLLQLLHILNERIKTKKNTTTVRDIYYGNIELFESQQMVEMLFQRIEAMFNVEKLFF 181
++ LL++L I+ + ++ ++ + RDIYY + F++Q +V+ I +F +
Sbjct: 75 RIDMLLRVLLIVQQLLQENRHASK-RDIYYMHPSAFKAQSIVDRAIGDICILFQCSRYNL 133
Query: 182 NVVSTQKGLIYSGPRLQIGSQLFHAECQLVPYDQGEMIVQQNQQL-----KVIVLEKDAI 236
NVVS GL+ + + + F L ++V++ + + ++V+EK+ +
Sbjct: 134 NVVSVGNGLVMGWLKFREAGRKFDCLNSLNTAYPVPVLVEEVEDIVSLAEYILVVEKETV 193
Query: 237 FQQLIQ---CQEMIKDWIIVTGKGYPDHLSRELLHKLDLSLQGNVQFHIFTDSDPYGIDI 293
FQ+L C+ I++TG+GYPD +R L L L ++ H D DPYG +I
Sbjct: 194 FQRLANDMFCK--TNRCIVITGRGYPDVSTRRFLRLLMEKL--HLPVHCLVDCDPYGFEI 249
Query: 294 VTKY 297
+ Y
Sbjct: 250 LATY 253
>7302468 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 331
Score = 69.7 bits (169), Expect = 7e-12
Identities = 51/206 (24%), Positives = 98/206 (46%), Gaps = 14/206 (6%)
Query: 101 SGGKLLRWRYPEFANTKKKCSKVPQLLQLLHILNERIKTKKNTTTVRDIYYGNIELFESQ 160
SG + +R + N + S + L + R++ + + TVR +YY N L SQ
Sbjct: 33 SGNVISEYRRVSYNNRGSRHSFCVLIYMLSRV--HRLQVRGGSFTVRGLYYDNPLLVRSQ 90
Query: 161 QMVEMLFQRIEAMFNVEKLFFNVVSTQKGLIYSGPRLQ------IGSQLFHAECQLVPYD 214
+ + M L +++ KGL+ RL + S L+ L P D
Sbjct: 91 SRIAEARLDVCRMLRTSPLSLGILAASKGLVAGDLRLLMTNGDVLDSSLYGGPLTL-PTD 149
Query: 215 QGEMIVQQNQQLKVIVLEKDAIFQQLIQCQ---EMIKDWIIVTGKGYPDHLSRELLHKLD 271
++ + V+++EK+++F+ L+ + +I++TGKGYPD +R ++H+
Sbjct: 150 PEKIDRIETLAEFVLIVEKESVFESLLSRNVFGTFERRFILITGKGYPDCCTRRIVHR-- 207
Query: 272 LSLQGNVQFHIFTDSDPYGIDIVTKY 297
L+ + + +I D+DP+G++I+ Y
Sbjct: 208 LTEENQLAAYILVDADPFGVEIMLVY 233
>At5g02820 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 427
Score = 66.2 bits (160), Expect = 8e-11
Identities = 47/186 (25%), Positives = 92/186 (49%), Gaps = 12/186 (6%)
Query: 118 KKCSKVPQLLQLLHILNERIKTKKNTTTVRDIYYGNIELFESQQMVEMLFQRIEAMFNVE 177
+K + ++L L+H ++ + T RD++Y +++LF+ Q + + + M
Sbjct: 141 RKTTITTRILALIH----QLCLRNIHVTKRDLFYTDVKLFQDQTQSDAVLDDVSCMLGCT 196
Query: 178 KLFFNVVSTQKG-----LIYSGPRLQIGSQLFHAECQLVPYDQGEMIVQQNQQLKVIVLE 232
+ NV++ +KG LI+S I + +P + + Q+ + ++++E
Sbjct: 197 RSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGKAIPPNIDRVGDMQSDAMFILLVE 256
Query: 233 KDAIFQQLIQCQEMIK-DWIIVTGKGYPDHLSRELLHKLDLSLQGNVQFHIFTDSDPYGI 291
KDA + +L + + + IIVT KG PD +R L K+ + L+ + DSDPYG+
Sbjct: 257 KDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMKMELK--LPVLALVDSDPYGL 314
Query: 292 DIVTKY 297
I++ Y
Sbjct: 315 KILSVY 320
>CE06363 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 425
Score = 59.7 bits (143), Expect = 7e-09
Identities = 48/186 (25%), Positives = 90/186 (47%), Gaps = 7/186 (3%)
Query: 116 TKKKCSKVPQLLQLLHILNERIKTKKNTTTVRDIYYGNIELFESQQMVEMLFQRIEAMFN 175
T K+ K+ + L LH + + ++ K +T R++YY + ++ +Q+ ++ + I + N
Sbjct: 85 TAKRKGKLDRDLHCLHQVYDLLENDKRSTK-RELYYEHKAVYGNQKYLDSSIKSICELLN 143
Query: 176 VEKLFFNVVSTQKGLIYSGPRLQIGSQ-LFHAECQLVPYDQGEMIVQQNQQLK-VIVLEK 233
+ N++S +G+I + + + A Q V + + ++V+EK
Sbjct: 144 ESRANLNILSCGRGIIRGAITFLVENVGVIDARVQEVLITDALLFSNIISEADFILVVEK 203
Query: 234 DAIFQQLIQ--CQEMIKDWIIVTGKGYPDHLSRELLHKLDLSLQGNVQFHIFTDSDPYGI 291
D FQ+L+ Q M I+ T KGYPD +R +L LS + + D+DP+GI
Sbjct: 204 DTTFQKLMDENFQAMFPRGILATSKGYPDIATRNVLKM--LSEKRKFPIYGLFDADPHGI 261
Query: 292 DIVTKY 297
+I Y
Sbjct: 262 EIYLTY 267
>ECU04g1110 [L] KOG2795 Catalytic subunit of the meiotic double strand break
transesterase
Length = 303
Score = 52.0 bits (123), Expect = 2e-06
Identities = 52/223 (23%), Positives = 97/223 (43%), Gaps = 34/223 (15%)
Query: 143 TTTVRDIYYGNIELFESQQMVEMLFQRIEAMFNVEKLFFNVVSTQKGLIYSGPRLQIGSQ 202
T R+I+Y ++ +F +Q +V L I + + K V +T KG+ ++G
Sbjct: 49 TRNEREIFYMDVNVFRTQSVVRRLVSSIASELQISKHDLGVRNTLKGIFIG----RLGFV 104
Query: 203 LFHA----------EC-QLVPYDQGEMIVQQNQQLKVIVLEKDAIFQQL---IQCQEMIK 248
H C QL+P D ++ K +V+EKD + Q++ I+ ++ ++
Sbjct: 105 RHHGLGMVEMSSKGGCPQLIP-DMSDIAEVLCDYKKTVVVEKDTVLQRIASEIEREKCLE 163
Query: 249 DWIIVTGKGYPDHLSRELLHKLDLSLQGNVQFHIFTDSDPYGIDIVTKYM--------VK 300
+ + V GKGYP + LL ++ F D DP+GI I Y ++
Sbjct: 164 EILFVCGKGYPCKNTVLLLKMIEHKTAVAGLF----DLDPFGIHIFCIYKYGSKETPDIR 219
Query: 301 CPRLIHKGVLLNQLITRNTESNSMLVLLPLSLRDATFAMGLIR 343
++ GV + ++ +N + V + L++ D L+R
Sbjct: 220 VETIMRIGVCMEDVLEKNAYKD---VFVKLNVHDLKMINRLVR 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,591,863
Number of Sequences: 60738
Number of extensions: 835661
Number of successful extensions: 2362
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2340
Number of HSP's gapped (non-prelim): 8
length of query: 401
length of database: 30,389,216
effective HSP length: 109
effective length of query: 292
effective length of database: 23,768,774
effective search space: 6940482008
effective search space used: 6940482008
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)