ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV3816 good T KOG2980 Signal transduction mechanisms Integral membrane protease of the rhomboid family involved in different forms of regulated intramembrane proteolysis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV3816 1294983 1296011 343
(343 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR101w [T] KOG2980 Integral membrane protease of the rhomboid f... 343 3e-94
Hs20127652_2 [T] KOG2980 Integral membrane protease of the rhomb... 82 9e-16
SPBC13E7.11 [T] KOG2980 Integral membrane protease of the rhombo... 73 7e-13
7303544 [T] KOG2980 Integral membrane protease of the rhomboid f... 70 3e-12
>YGR101w [T] KOG2980 Integral membrane protease of the rhomboid family
involved in different forms of regulated intramembrane
proteolysis
Length = 346
Score = 343 bits (879), Expect = 3e-94
Identities = 161/276 (58%), Positives = 204/276 (73%), Gaps = 2/276 (0%)
Query: 62 RFFTTTSNAKINLRDMFNG-SQSRYTRLNRFQQYNRGSGNSSELIKLTLYGVLFMGGTLM 120
RFF+ TS K + +FN + +RY RLNRFQQY + + L +T+ G+ M G
Sbjct: 64 RFFSQTSILKSRWKPIFNEETTNRYVRLNRFQQYQQQRSGGNPLGSMTILGLSLMAGIYF 123
Query: 121 LAPYLFQ-IPPFSYFKRHPTHLPYAIIGINLAVFGLWQLPRFWGPLQRYALLQKDHIYSK 179
+PYLF+ +PPF+YFK HP +L YA++GIN+AVFGLWQLP+ W LQ+Y LLQKD++ SK
Sbjct: 124 GSPYLFEHVPPFTYFKTHPKNLVYALLGINVAVFGLWQLPKCWRFLQKYMLLQKDYVTSK 183
Query: 180 WSLLGSAFSHQEFWHLGMNMICLFSFGTSLVTMLGPGNFTSLYLNSAIAGSLFSLWYPRF 239
S++GSAFSHQEFWHLGMNM+ L+SFGTSL TMLG NF SLY+NSAIAGSLFSLWYP+
Sbjct: 184 ISIIGSAFSHQEFWHLGMNMLALWSFGTSLATMLGASNFFSLYMNSAIAGSLFSLWYPKL 243
Query: 240 AKIAMMGPXXXXXXXXXXXXXXXXYLIPNAKMMFFIFPVPFGAWFTFLGLVTWNVVGCAL 299
A++A++GP YL P+AK++ F+FPVP GAW FL V WN GCAL
Sbjct: 244 ARLAIVGPSLGASGALFGVLGCFSYLFPHAKILLFVFPVPGGAWVAFLASVAWNAAGCAL 303
Query: 300 RWGSFDYAAHLGGSAMGVLYGWLVAKSIQKQREKRL 335
RWGSFDYAAHLGGS MGVLYGW ++K+++KQR++RL
Sbjct: 304 RWGSFDYAAHLGGSMMGVLYGWYISKAVEKQRQRRL 339
>Hs20127652_2 [T] KOG2980 Integral membrane protease of the rhomboid family
involved in different forms of regulated intramembrane
proteolysis
Length = 208
Score = 82.4 bits (202), Expect = 9e-16
Identities = 54/189 (28%), Positives = 81/189 (42%), Gaps = 3/189 (1%)
Query: 145 IIGINLAVFGLWQLPRFWGPLQRYALLQKDHIYSKWSLLGSAFSHQEFWHLGMNMICLFS 204
II N+ VF LW++P + RY +L S FSH +H+ NM L+S
Sbjct: 1 IIAANVLVFCLWRVPSLQRTMIRYFTSNPASKVLCSPMLLSTFSHFSLFHMAANMYVLWS 60
Query: 205 FGTSLVTMLGPGNFTSLYLNSAIAGSLFSLWYPRFAKIAMMGPXXXXXXXXXXXXXXXXY 264
F +S+V +LG F ++YL++ + + S Y GP
Sbjct: 61 FSSSIVNILGQEQFMAVYLSAGVISNFVS--YVGKVATGRYGPSLGASGAIMTVLAAVCT 118
Query: 265 LIPNAKMMFFIFPV-PFGAWFTFLGLVTWNVVGCALRWGSFDYAAHLGGSAMGVLYGWLV 323
IP ++ P+ F A ++ + G L W FD+AAHLGG+ G+ Y
Sbjct: 119 KIPEGRLAIIFLPMFTFTAGNALKAIIAMDTAGMILGWKFFDHAAHLGGALFGIWYVTYG 178
Query: 324 AKSIQKQRE 332
+ I K RE
Sbjct: 179 HELIWKNRE 187
>SPBC13E7.11 [T] KOG2980 Integral membrane protease of the rhomboid family
involved in different forms of regulated intramembrane
proteolysis
Length = 180
Score = 72.8 bits (177), Expect = 7e-13
Identities = 45/118 (38%), Positives = 65/118 (54%), Gaps = 9/118 (7%)
Query: 124 YLFQIPPFSYFKRHPTHLPYAIIGINLAVFGLWQLPRFWGP---LQRYALLQKDHIYSKW 180
Y F IP S L +IIGIN+ VF LW+ PRF LQ+YA++ I
Sbjct: 67 YGFPIPQTS-----SRSLVLSIIGINVGVFALWRAPRFSHLNRFLQKYAVMNPIFINMP- 120
Query: 181 SLLGSAFSHQEFWHLGMNMICLFSFGTSLVTMLGPGNFTSLYLNSAIAGSLFSLWYPR 238
S++ SAFSHQ WHL NM+ +SF ++V + G F + Y++S + ++ SL + R
Sbjct: 121 SMIVSAFSHQSGWHLLFNMVAFYSFAPAIVDVFGNNQFVAFYISSILFSNVASLLHHR 178
>7303544 [T] KOG2980 Integral membrane protease of the rhomboid family
involved in different forms of regulated intramembrane
proteolysis
Length = 351
Score = 70.5 bits (171), Expect = 3e-12
Identities = 46/176 (26%), Positives = 71/176 (40%), Gaps = 3/176 (1%)
Query: 145 IIGINLAVFGLWQLPRFWGPLQRYALLQKDHIYSKWSLLGSAFSHQEFWHLGMNMICLFS 204
I+ NL F +W++P + Y W + S FSH HL NM + S
Sbjct: 151 ILLCNLVAFAMWRVPALKSTMITYFTSNPAAKVVCWPMFLSTFSHYSAMHLFANMYVMHS 210
Query: 205 FGTSLVTMLGPGNFTSLYLNSAIAGSLFSLWYPRFAKIAMMGPXXXXXXXXXXXXXXXXY 264
F + LG F ++YL++ + SL S+ Y A M Y
Sbjct: 211 FANAAAVSLGKEQFLAVYLSAGVFSSLMSVLYKAATSQAGMSLGASGAIMTLLAYVCTQY 270
Query: 265 LIPNAKMMFFIFP-VPFGAWFTFLGLVTWNVVGCALRWGSFDYAAHLGGSAMGVLY 319
P+ ++ P + F A L+ + G + W FD+AAHLGG+ G+ +
Sbjct: 271 --PDTQLSILFLPALTFSAGAGIKVLMGIDFAGVVMGWKFFDHAAHLGGAMFGIFW 324
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.329 0.142 0.463
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,041,600
Number of Sequences: 60738
Number of extensions: 745018
Number of successful extensions: 1766
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1759
Number of HSP's gapped (non-prelim): 5
length of query: 343
length of database: 30,389,216
effective HSP length: 107
effective length of query: 236
effective length of database: 23,890,250
effective search space: 5638099000
effective search space used: 5638099000
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)