ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4051 suspect: %id DO KOG2165 Cell cycle control, cell division, chromosome partitioning Anaphase-promoting complex (APC), subunit 2
r_klactIV4051 suspect: %id DO KOG2165 Posttranslational modification, protein turnover, chaperones Anaphase-promoting complex (APC), subunit 2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4051 1375538 1373448 -697
(697 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR127c [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2 231 4e-60
7291701 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2 80 1e-14
SPBP23A10.04 [DO] KOG2165 Anaphase-promoting complex (APC) subun... 74 8e-13
Hs7019327 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2 73 1e-12
At2g04660 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2 66 2e-10
>YLR127c [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2
Length = 853
Score = 231 bits (588), Expect = 4e-60
Identities = 184/744 (24%), Positives = 335/744 (44%), Gaps = 100/744 (13%)
Query: 21 SNLEEEVDMVLAWLNPT-----FQPQIPTLRIKQCLKVVLEQSDERTRF----------- 64
+N+ ++++ +L W++P Q + P+LRIK +KV+ + + +
Sbjct: 28 TNIVDDLNSLLTWMSPNDAKSNHQLRPPSLRIKNIIKVLFPNNATTSPYSMINTSQANNS 87
Query: 65 -----------------LIEQYYLTLIRTHFFLQFDDIVNWKDMIKLEKLYISKVEFM-- 105
++++Y+ +R HFFL F++I KD+ + E Y + ++
Sbjct: 88 IVNEGNTNKELQLQLFSTLKEFYIFQVRYHFFLHFNNINYLKDIQRWENYYEFPLRYVPI 147
Query: 106 FGTSVYWIKEEMISFKRLLMKKNNEFRKQLHAKLESHILDNDLVRFDQMYQWLAPAFDG- 164
F +V E+ S + L+ +N +F+ L +L+ I+D+D D + QWL A
Sbjct: 148 FDVNVNDWALELNSLRHYLLNRNIKFKNNLRTRLDKLIMDDDFDLADNLIQWLKSANGSL 207
Query: 165 QEIEFNVRIINLKVDQMSQKLMKNKIDDRYLVMNTYNHFIKDFWSKFSKLL-IIEDDHEL 223
E V + K+++ + M + R+++M T+N FI +WS+FSKL+ EDDHEL
Sbjct: 208 SSTELIVNALYSKINKFCEDNMSRVWNKRFMIMETFNKFINQYWSQFSKLVGCPEDDHEL 267
Query: 224 TAIIYQSFEKNYIKYKCDEFYTDIVPKFPASRKCLLELRSILNKDIKTVGTKVLETLYHG 283
T ++ FE N+++ + +E + V +P S+ LLELR I+ KD K T ++ T
Sbjct: 268 TTTVFNCFESNFLRIRTNEIFDICVLAYPDSKVTLLELRKIM-KDFKDY-TNIVTTFLSD 325
Query: 284 FVSRFLTSSLLTCEILYYYIKTVKCLKIIDPMGICLRSLSKAVRVYLNPRPDIIKTLLLG 343
F L S+ T + L Y+KT+K ++DP G CL S++ V+ Y R ++ LL
Sbjct: 326 FKKYILNPSVTTVDALLRYVKTIKAFLVLDPTGRCLHSITTFVKPYFQERKHLVNVLLYA 385
Query: 344 IFPFQNNE-RFKIASSTDGSSVHLEKLEQFSREVGDFSMGPELPTALPWFNQPHLPRCTY 402
+ E + KI + D ++ L ++ + D + + P L
Sbjct: 386 MLDLPEEELKEKINFNVDMKAL-LSLVD--TLHDSDINQDTNITKRDKNKKSPFLWNLKV 442
Query: 403 DGD---------------DQLLKQYLNWVPEPPRIKLDVEDFADNDGKYVPPVDLIHVLL 447
G + +L Y+ WVPEP + N +L VLL
Sbjct: 443 KGKRELNKDLPIRHAMLYEHILNYYIAWVPEPN------DMIPGNIKSSYIKTNLFEVLL 496
Query: 448 DVLESKRTLVDDLLGVVSGKFIESEEYSLDPEWQRIMDI----------------ILNHL 491
D+ ES+ + + +++ + + Y+LD +W R + + I N +
Sbjct: 497 DLFESREFFISEFRNLLTDRLFTLKFYTLDEKWTRCLKLIREKIVKFTETSHSNYITNGI 556
Query: 492 EGTRKGTVTSEEADLTHLNDVDIMLEDLRLSSQFRNQVAHKNNSHFENFPHIKILSKLYW 551
G + T + +AD ++LN +D+ML D++ S + ++ FP K +S LYW
Sbjct: 557 LGLLETTAPAADADQSNLNSIDVMLWDIKCSEELCRKMHEVAGLDPIIFP--KFISLLYW 614
Query: 552 RHYQNLNGRLVTTKYKWDSEMEPLIQKLTKVYERLNTGRTLKFDTGSSSRVSINIVTKSG 611
++ + G + ++E +QK + +Y +L GR L+ +V I + K G
Sbjct: 615 KYNCDTQGS-NDLAFHLPIDLERELQKYSDIYSQLKPGRKLQL-CKDKGKVEIQLAFKDG 672
Query: 612 ERRFFKVTMEQYLVISHFQNETNGRNAPGMMADCNYVIPERRYTLKDLHKMTSMPRQKIL 671
+ V++EQ VI+ F ++P C +L+ L + ++ ++
Sbjct: 673 RKLVLDVSLEQCSVINQF-------DSPNDEPIC--------LSLEQLSESLNIAPPRLT 717
Query: 672 DILSFWQQKEIVTKHSDDSYSVQE 695
+L FW QK ++ K + +YSV E
Sbjct: 718 HLLDFWIQKGVLLK-ENGTYSVIE 740
>7291701 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2
Length = 802
Score = 79.7 bits (195), Expect = 1e-14
Identities = 93/479 (19%), Positives = 196/479 (40%), Gaps = 95/479 (19%)
Query: 222 ELTAIIYQSFEKNYIKYKCDEFYTDIVPKFPASRKCLLELRSILNK-DIKTVGTKVLETL 280
+LT +Y++F ++ I +F++ I+ +P S + +L+ + K D++ T E+L
Sbjct: 311 KLTYFMYETFAQSVI----GQFFSIIID-YPDSIPAIDDLKICMEKIDMRVYLT---ESL 362
Query: 281 YHGFVSRFLTSSLLTCEILYYYIKTVKCLKIIDPMGICLRSLSKAVRVYLNPRPDIIKTL 340
+ +R L + T +IL Y+ +K ++ +D G+ L ++ ++ YL R D ++ +
Sbjct: 363 RNSLEARILHPGVNTMDILTGYVAAIKAIRHLDSTGVILEMVTAPIKDYLRKRNDTVRRV 422
Query: 341 LLGIFPFQNNERFKIASSTDGSSVHLEKLEQFSREVGDFSMGPELPTALPWFNQPHLPRC 400
+ G+ E G + EL + C
Sbjct: 423 VTGL-----------------------------TEEGPTDLSEELAKG------ETIKEC 447
Query: 401 TYDGDDQLLKQYLNWVPEPPRIKLDVEDFADNDGKYVPPVDLIHVLLDVLESKRTLVDDL 460
G D+ + NW P+P I + + N + + D+I +++D+ SK + +
Sbjct: 448 KDSGTDEF-SNWENWQPDPFGIDASIMQY--NSSRKMRSADIISMVVDIYGSKELFMTEY 504
Query: 461 LGVVSGKFIESEEYSLDPEWQRIMDIILNHLEGTRKGTVTSEEADLTHLNDVDIMLEDLR 520
+++ + + +++ + E + + + + E L+ ++ML+D+
Sbjct: 505 RNLMADRLLAQLDFNSEKEIRNLELLKIRFGESL--------------LHSCEVMLKDVT 550
Query: 521 LSSQFRNQVAHKNNSHFENFPH---IKILSKLYWRHYQNLNGRLVTTKYKWDSEMEPLIQ 577
S + + H + EN I+S +W + + +L E+E +
Sbjct: 551 DSKRINAHI-HSDGDRTENQLFDISSLIVSAQFWPSFNKESLQL-------PEEIENEFK 602
Query: 578 KLTKVYERLNTGRTLKFDTGSSSRVSINIVTKSGERRFFKVTMEQY-LVISHFQNETNGR 636
K TK YE RTL + T + +NI + G+R V ++I HFQ +
Sbjct: 603 KYTKAYEAYKGNRTLNWRTVTGR---VNIEIEIGDRTMEMVVSPILAVIIYHFQTKNE-- 657
Query: 637 NAPGMMADCNYVIPERRYTLKDLHKMTSMPRQKILDILSFWQQKEIVTKHSDDSYSVQE 695
+T++DL +T +P + LSFWQ ++++ + +++ E
Sbjct: 658 -----------------WTIEDLSSITKVPASALRRRLSFWQNHGLISETTPGIFTLLE 699
>SPBP23A10.04 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2
Length = 696
Score = 73.9 bits (180), Expect = 8e-13
Identities = 107/504 (21%), Positives = 188/504 (37%), Gaps = 110/504 (21%)
Query: 196 VMNTYNHFIKDFWSKFSKLLIIEDDHELTAIIYQSFEKNYIKYKCDEFYTDIVPKFPASR 255
+ + ++ +S + ++++ DH ++ I+ N + DIV +P S
Sbjct: 187 IRTEFGVLVEHVFSLSNPAVLVQLDHLVSQILAHLRSDNIL---------DIVLHYPNSL 237
Query: 256 KCLLELRSILNKDIKTVGTKVLETLYHGFVSRFLTSSLLTCEILYYYIKTVKCLKIIDPM 315
+ +LR + + K + ET S LT+S + IL +Y+ T++C +DP
Sbjct: 238 GAIEDLRLVARQ--KQQRQYLTETFVKDCTSSILTASSDSSYILLFYVSTIRCFVALDPP 295
Query: 316 GICLRSLSKAVRVYLNPRPDIIKTLLLGIFPFQNNERFKIASSTDGSSVHLEKLEQFSRE 375
G+ L +K +R +LN R D K L+ +F DG +L Q
Sbjct: 296 GVLLDKAAKPIRSFLNEREDAYKCLVSLLF-------------VDGEKGLRSELSQ---- 338
Query: 376 VGDFSMGPELPTALPWFNQPHLPRCTYDGDDQLLKQYLNWVPEPPRIKLDVEDFADNDGK 435
+P D Y +W+P+P D + D
Sbjct: 339 ---------------------IPTENIDSTTDRFDNY-HWMPDPIDAAPDFKKPTDR--- 373
Query: 436 YVPPVDLIHVLLDVLESKRTLVDDLLGVVSGKFIESEEYSLDPEWQRIMDIILNHLEGTR 495
D++ L+ + +SK LV +L +++ + ++ +Y + E + I + E
Sbjct: 374 -----DVVGSLISIFKSKEPLVKELQLLLADRLLQLTDYHYEVEAKNIEFLKYRFGE--- 425
Query: 496 KGTVTSEEADLTHLNDVDIMLEDLRLSSQFRNQVAHKNNSHFENFPHIKILSKLYWR--- 552
T L +ML D+ +S+F +Q H N + H+ ILS+L+W
Sbjct: 426 -----------TVLQMCSVMLNDIE-NSRFIDQSIHMEN-YVSKGLHVTILSRLFWPTLS 472
Query: 553 -HYQNLNGRLVTTKYKWDSEMEPLIQKLTKVYERLNTGRTLKFDTGSSSRVSINIVTKSG 611
Y +L G L K + D+ E Y R L F S V + I +
Sbjct: 473 VRYFHLPGPL---KKELDAYAEE--------YRERKRKRELVFLPNLGS-VELEIELED- 519
Query: 612 ERRFFKVTMEQYLVISHFQNETNGRNAPGMMADCNYVIPERRYTLKDLHKMTSMPRQKIL 671
VT EQ IS F+ + ++ ++ P++ +
Sbjct: 520 RTLTLTVTPEQAAFISLFEETST-------------------LHIEKAAELLDQPKEIVE 560
Query: 672 DILSFWQQKEIVTKHSDDSYSVQE 695
L FW ++T DD Y V+E
Sbjct: 561 RHLKFWLHHRVLTDIGDDRYRVRE 584
>Hs7019327 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2
Length = 822
Score = 73.2 bits (178), Expect = 1e-12
Identities = 94/481 (19%), Positives = 194/481 (39%), Gaps = 103/481 (21%)
Query: 227 IYQSFEKNYIKYKCDEFYTDIVPKFPASRKCLLELRSILNKDIKTVGTKVLETLYHGFVS 286
+ + F + Y + +E ++ IV FP SR + +L+ L + + ++L +L +
Sbjct: 328 VQRFFYRIYASLRIEELFS-IVRDFPDSRPAIEDLKYCLERTDQR--QQLLVSLKAALET 384
Query: 287 RFLTSSLLTCEILYYYIKTVKCLKIIDPMGICLRSLSKAVRVYLNPRPDIIKTLLLGIFP 346
R L + TC+I+ YI +K L+++DP + L + +R YL R D ++ ++ G
Sbjct: 385 RLLHPGVNTCDIITLYISAIKALRVLDPSMVILEVACEPIRRYLRTREDTVRQIVAG--- 441
Query: 347 FQNNERFKIASSTDGS---SVHLEKLEQFSREVGDFSMGPELPTALPWFNQPHLPRCTYD 403
+ +DG+ +V L K + S E G D
Sbjct: 442 --------LTGDSDGTGDLAVELSKTDPASLETGQ------------------------D 469
Query: 404 GDDQLLKQYLNWVPEPPRIKLDVEDFADNDGKYVPPVDLIHVLLDVLESKRTLVDDLLGV 463
+D + +WVP+P V+ D+I +L+ + SK +++ +
Sbjct: 470 SEDD-SGEPEDWVPDP------VDADPGKSSSKRRSSDIISLLVSIYGSKDLFINEYRSL 522
Query: 464 VSGKFIESEEYSLDPEWQRIMDIILNHLEGTRKGTVTSEEADLTHLNDVDIMLEDLRLSS 523
++ + + +S + E + + + L E ++ ++ML+D+ S
Sbjct: 523 LADRLLHQFSFSPEREIRNVELLKLRFGEAP--------------MHFCEVMLKDMADSR 568
Query: 524 QFRNQVAHKNNSH-FENFP----HIKILSKLYWRHYQNLNGRLVTTKYKWDSEMEPLIQK 578
+ + ++ E P + ILS +W +++ K + ++ ++
Sbjct: 569 RINANIREEDEKRPAEEQPPFGVYAVILSSEFWPPFKD-------EKLEVPEDIRAALEA 621
Query: 579 LTKVYERLNTGRTLKFDTGSSSRVSINIVTKSGE----RRFFKVTMEQYLVISHFQNETN 634
K YE+L RTL S + ++ +VT E VT Q +++ +FQ++ +
Sbjct: 622 YCKKYEQLKAMRTL------SWKHTLGLVTMDVELADRTLSVAVTPVQAVILLYFQDQAS 675
Query: 635 GRNAPGMMADCNYVIPERRYTLKDLHKMTSMPRQKILDILSFWQQKEIVTKHSDDSYSVQ 694
+TL++L K MP + +S W Q+ ++ + ++SV
Sbjct: 676 -------------------WTLEELSKAVKMPVALLRRRMSVWLQQGVLREEPPGTFSVI 716
Query: 695 E 695
E
Sbjct: 717 E 717
>At2g04660 [DO] KOG2165 Anaphase-promoting complex (APC) subunit 2
Length = 873
Score = 66.2 bits (160), Expect = 2e-10
Identities = 55/237 (23%), Positives = 103/237 (43%), Gaps = 33/237 (13%)
Query: 246 DIVPKFPASRKCLLELRSILNKDIKTVGTKVLETLYHGFVSRFLTSSLLTCEILYYYIKT 305
+I+ +P S + +L+ L + +K++E+ R LT+ T +IL+ Y+ T
Sbjct: 370 EIIVDYPESSPAIEDLKQCL--EYTGQHSKLVESFISSLKYRLLTAGASTNDILHQYVST 427
Query: 306 VKCLKIIDPMGICLRSLSKAVRVYLNPRPDIIKTLLLGIFPFQNNERFKIASSTDGSSVH 365
+K L+ IDP G+ L ++ + +R YL R D IK ++ + + D
Sbjct: 428 IKALRAIDPAGVFLEAVGEPIRDYLRGRKDTIKCIVTMLTDGSGGNANGSGNPGDSLLEE 487
Query: 366 LEKLEQFSREVG---DFSMGPELPTALPWFNQPHLPRCTYDGDDQLLKQYLNWVPEPPRI 422
L + E+ VG DF + D Q W P+P +
Sbjct: 488 LMRDEESQENVGFDDDF----------------------HTDDKQAWINASRWEPDP--V 523
Query: 423 KLDVEDFADNDGKYVPPVDLIHVLLDVLESKRTLVDDLLGVVSGKFIESEEYSLDPE 479
+ D + + K VD++ +L+D++ SK LV++ +++ K + +Y +D E
Sbjct: 524 EADPLKGSLSQRK----VDILGMLVDIIGSKEQLVNEYRVMLAEKLLNKTDYDIDTE 576
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.139 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,386,051
Number of Sequences: 60738
Number of extensions: 1975773
Number of successful extensions: 4826
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4811
Number of HSP's gapped (non-prelim): 11
length of query: 697
length of database: 30,389,216
effective HSP length: 113
effective length of query: 584
effective length of database: 23,525,822
effective search space: 13739080048
effective search space used: 13739080048
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)