ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4115 good M KOG2686 Cell wall/membrane/envelope biogenesis Choline kinase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4115 1392680 1394290 537
(537 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR133w [M] KOG2686 Choline kinase 471 e-133
YDR147w [M] KOG2686 Choline kinase 308 1e-83
SPAC13G7.12c [M] KOG2686 Choline kinase 169 1e-41
At1g74320 [M] KOG2686 Choline kinase 136 7e-32
At4g09760 [M] KOG2686 Choline kinase 127 3e-29
At1g71697 [M] KOG2686 Choline kinase 119 2e-26
At2g26830 [I] KOG4720 Ethanolamine kinase 116 1e-25
Hs6978649 [M] KOG2686 Choline kinase 108 2e-23
CE27113 [M] KOG2686 Choline kinase 108 3e-23
7302122 [M] KOG2686 Choline kinase 95 3e-19
CE07553 [I] KOG4720 Ethanolamine kinase 92 3e-18
At1g34100_2 [M] KOG2686 Choline kinase 91 3e-18
Hs4557455 [M] KOG2686 Choline kinase 89 2e-17
7293191 [I] KOG4720 Ethanolamine kinase 77 7e-14
Hs8922650 [I] KOG4720 Ethanolamine kinase 66 2e-10
>YLR133w [M] KOG2686 Choline kinase
Length = 582
Score = 471 bits (1213), Expect = e-133
Identities = 243/483 (50%), Positives = 316/483 (65%), Gaps = 17/483 (3%)
Query: 63 ESISVPYVKSTLDISLPHDLLQEDFKNLIIALRVKHWY---KHDLDASMLRFTRISGAMT 119
E I +P+VK TLD SLP D L++D NLI +L++ WY K A + +ISGAMT
Sbjct: 99 EYIEIPFVKETLDASLPSDYLKQDILNLIQSLKISKWYNNKKIQPVAQDMNLVKISGAMT 158
Query: 120 NAIFKVEYDNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQF 179
NAIFKVEY LPSLL+R+YGPN++++IDREYELQ+LARLS+++IGPSLYGCF NGR EQF
Sbjct: 159 NAIFKVEYPKLPSLLLRIYGPNIDNIIDREYELQILARLSLKNIGPSLYGCFVNGRFEQF 218
Query: 180 LENSHTLTKSDIRDWKTSQRIARRMKELHSGVPLLSKELKDEPATWKRIEKWTNTLAK-S 238
LENS TLTK DIR+WK SQRIARRMKELH GVPLLS E K+ A W++I +W T+ K
Sbjct: 219 LENSKTLTKDDIRNWKNSQRIARRMKELHVGVPLLSSERKNGSACWQKINQWLRTIEKVD 278
Query: 239 SWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPS-----YRKLVFCHNDAQYGNLLF 293
W+ D NI L+ +W +F+ IV +Y KWL + + L+FCHNDAQYGNLLF
Sbjct: 279 QWVGDPKNIENSLLCENWSKFMDIVDRYHKWLISQEQGIEQVNKNLIFCHNDAQYGNLLF 338
Query: 294 AAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDF 353
APV+ + +++IINP QEQ+QD+KLVVIDF
Sbjct: 339 TAPVMNTPS---LYTAPSSTSLTSQSSSLFPSSSNVIVDDIINPPKQEQSQDSKLVVIDF 395
Query: 354 EYGGPNPPAFDLANHLSEWMHDY-NCSTPYVVFHDRFPTHEEMLNFIYSYLSHLNPKRKH 412
EY G NP A+DLANHLSEWM+DY N P+ DR+P E++LNF+YSY+SHL K
Sbjct: 396 EYAGANPAAYDLANHLSEWMYDYNNAKAPHQCHADRYPDKEQVLNFLYSYVSHLRGGAKE 455
Query: 413 -MEEEVCELYNDILDWRGSVSLHWALWGIIQSGELTTEIDESEKTEE--GPGGEKYIITV 469
++EEV LY I+ WR +V L W+LW I+QSG+L + + T E GP G+KYII
Sbjct: 456 PIDEEVQRLYKSIIQWRPTVQLFWSLWAILQSGKLEKKEASTAITREEIGPNGKKYIIKT 515
Query: 470 DKLSSPTIDGDDSGEEENAGVDIDTFEYLLYSKNKLEIFYSDLIQLNVIDKSEVLNADDL 529
+ SP D ++ +E AGV IDTF+Y+ Y ++K+ +F+ DLI L +I + E N
Sbjct: 516 EP-ESPEEDFVENDDEPEAGVSIDTFDYMAYGRDKIAVFWGDLIGLGIITEEECKNFSSF 574
Query: 530 KVI 532
K +
Sbjct: 575 KFL 577
>YDR147w [M] KOG2686 Choline kinase
Length = 534
Score = 308 bits (789), Expect = 1e-83
Identities = 176/494 (35%), Positives = 260/494 (52%), Gaps = 86/494 (17%)
Query: 58 SLVLGESISVPYVKSTLDISLPHDLLQEDFKNLIIALRVKHWY-----KHDLDASMLRFT 112
S + + + YV + LD SLP ++D N++ +L + W + L+ ++L T
Sbjct: 79 SQTANDFLKLAYVNAKLDPSLPSQYFKQDIINVLQSLEIPGWSVPGSKESSLNKNLLTLT 138
Query: 113 RISGAMTNAIFKVEYDNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSLYGCFE 172
+I GA+TN I+K+ Y NLP LL+R++G +++SVIDREYEL+V+ARLS +GP L G FE
Sbjct: 139 QIKGALTNVIYKIHYPNLPPLLMRIFGDSIDSVIDREYELKVIARLSFYDLGPKLEGFFE 198
Query: 173 NGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHSGVPLLSKELKDEPATWKRIEKWT 232
NGR E+++E S T T++D D TS +IA+++KELH VPL KE+ D+P+ W ++W
Sbjct: 199 NGRFEKYIEGSRTSTQADFIDRDTSIKIAKKLKELHCTVPLTHKEITDQPSCWTTFDQWI 258
Query: 233 NTL-AKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPSYR-------------- 277
+ + W+ +N NI++ L SSW+ FL YK+WL ++
Sbjct: 259 KLIDSHKEWVSNNVNISENLRCSSWNFFLKSFKNYKRWLYNDSAFTSKLLREDDKDSMIN 318
Query: 278 ---KLVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEI 334
K+VFCHND Q+GNLLF +
Sbjct: 319 SGLKMVFCHNDLQHGNLLFKS--------------------------------------- 339
Query: 335 INPSIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTPYVVFHDRFPTHEE 394
++ L +IDFEY GPNP FDL+NHL+EWM DYN + D++P E+
Sbjct: 340 ---KGKDDISVGDLTIIDFEYAGPNPVVFDLSNHLNEWMQDYNDVQSFKSHIDKYPKEED 396
Query: 395 MLNFIYSYLSHLNPKR-KHMEEEVCELYNDILDWRGSVSLHWALWGIIQSGELTTE-IDE 452
+L F SY++H+N K +EV LYN I++WR L W LW ++QSG L + E
Sbjct: 397 ILVFAQSYINHMNENHVKIASQEVRILYNLIIEWRPCTQLFWCLWALLQSGRLPQRPLIE 456
Query: 453 SEK---TEEGPGGEKYIITVDKLSSPTIDGDDSGEEENAGVDI---DTFEYLLYSKNKLE 506
EK + G G E +++ + +EN D D+F YL + K K+
Sbjct: 457 GEKLMSEKAGLGDETHLM-------------EHKNKENGKYDCSEDDSFNYLGFCKEKMS 503
Query: 507 IFYSDLIQLNVIDK 520
+F+ DLI L VIDK
Sbjct: 504 VFWGDLITLGVIDK 517
>SPAC13G7.12c [M] KOG2686 Choline kinase
Length = 456
Score = 169 bits (428), Expect = 1e-41
Identities = 135/475 (28%), Positives = 209/475 (43%), Gaps = 81/475 (17%)
Query: 71 KSTLDISLPHDLLQEDFKNLIIALRVKHWYKHDLD-ASMLRFTRISGAMTNAIFKV---E 126
K +LD + P + + +I L + W + L + L RISGA+TNA++ V E
Sbjct: 24 KGSLDCNDPDPIFRHKVLVIIHKLAISSWNRIPLTLCNKLHIKRISGALTNAVYYVAPPE 83
Query: 127 YDNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLENSHTL 186
+ P LL+R+YGP+VE I+R+ EL+ L RL+ +IGP L G F NGR EQ++E S TL
Sbjct: 84 GYHAPKLLLRIYGPHVELFINRQVELENLKRLARHNIGPYLIGEFSNGRFEQYME-STTL 142
Query: 187 TKSDIRDWKTSQRIARRMKELHSGVPLLSKELKDEPATWKRIEKW-TNTLAKSSWIQDND 245
T IRD K S + RR+ ELH+ + L E+ + PA WK W AK + +
Sbjct: 143 TCKTIRDPKLSIYVGRRLCELHNFILLHPHEVLEMPAAWKNCLVWLPKAKAKILGRKHSL 202
Query: 246 NINKYLMVSSWDQFLSIVAKYKKWLQQKPSY--RKLVFCHNDAQYGNLLFAAPVIPVTAD 303
I M + + F + + +W + K + K+VF HND QYGNLL
Sbjct: 203 AITSEFMKTLEEDFNAYYNWFVEWSRDKKDWFGLKMVFSHNDTQYGNLLKI--------- 253
Query: 304 XXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPNPPAF 363
+ SI + LV +DFEY GPN AF
Sbjct: 254 -----------------------------KAKKRSIPLSQKHRTLVPVDFEYAGPNLCAF 284
Query: 364 DLANHLSEWMHDYNCST-PYVVFHDRFPTHEEMLNFIYSYLSH----------------- 405
DLAN+ +EWM DY+ T Y++ R+P ++Y+
Sbjct: 285 DLANYFAEWMADYHHPTHNYLMDRSRYPDFNARKLVYHAYVEQSAVINDLLEIEDASLLK 344
Query: 406 ---LNPKRKHMEEEVCELYNDILDWRGSVSLHWALWGIIQSGELTTEIDESEKTEEGPGG 462
+ + E+++ L + + ++ WALWGI+Q E E ++ + +
Sbjct: 345 TDISDELKNTFEKQIMNLEESVRAISPAANIGWALWGILQCLEEDDEWEDLSVSSQ---- 400
Query: 463 EKYIITVDKLSSPTIDGDDSGEEENAGVDIDTFEYLLYSKNKLEIFYSDLIQLNV 517
D+ ++G + +F+Y+ YS K ++FY L +
Sbjct: 401 -----VADRPEKQLVEGSTV-----PPIGTSSFDYIGYSSEKFDLFYEGCAALGL 445
>At1g74320 [M] KOG2686 Choline kinase
Length = 348
Score = 136 bits (343), Expect = 7e-32
Identities = 106/375 (28%), Positives = 170/375 (45%), Gaps = 73/375 (19%)
Query: 104 LDASMLRFTRISGAMTNAIFKVEY---DNLPS--LLVRVYGPNVESVIDREYELQVLARL 158
+D+ L+ + GAMTN +F++++ + PS +LVR+YG VE DRE E++ +
Sbjct: 34 IDSKALQVIPLKGAMTNEVFQIKWPTREKGPSRKVLVRIYGEGVEIFFDREDEIRTFEFM 93
Query: 159 SIQHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHS-GVPLLSKE 217
S GP L G F NGRIE+FL ++ TL+ D+RD + S RIA RMKE H +P K
Sbjct: 94 SKHGHGPLLLGRFGNGRIEEFL-HARTLSACDLRDPEISGRIATRMKEFHGLEMPGAKKA 152
Query: 218 LKDEPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPSYR 277
L W R+ W + + ++ + +M + ++++ K +
Sbjct: 153 L-----LWDRLRNWLTACKRLASPEEAKSFRLDVM----EMEINMLEK-----SLFDNDE 198
Query: 278 KLVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINP 337
+ FCHND QYGN++
Sbjct: 199 NIGFCHNDLQYGNIMM-------------------------------------------- 214
Query: 338 SIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTPYVVFHDRFPTHEEMLN 397
+ + +ID+EY NP A+D+ANH E DY+ TP+++ + ++P EE
Sbjct: 215 ----DEETKAITIIDYEYSCYNPVAYDIANHFCEMAADYHTETPHIMDYSKYPGVEERQR 270
Query: 398 FIYSYLSHLNPKRKHMEEEVCELYNDILDWRGSVSLHWALWGIIQSGELTTEIDESEKTE 457
F+ +Y+S+ + K + V +L D+ + + L W LWGII E EID
Sbjct: 271 FLKTYMSYSD--EKPSDTMVKKLLEDVEKYTLASHLIWGLWGII--SEHVNEIDFDYMEY 326
Query: 458 EGPGGEKYIITVDKL 472
E+Y +T +L
Sbjct: 327 ARQRFEQYWLTKPRL 341
>At4g09760 [M] KOG2686 Choline kinase
Length = 346
Score = 127 bits (320), Expect = 3e-29
Identities = 97/380 (25%), Positives = 173/380 (45%), Gaps = 72/380 (18%)
Query: 85 EDFKNLIIALRVKHWYKHDLDASMLRFTRISGAMTNAIFKVEYDNLPS------LLVRVY 138
++ + ++ AL K W D L + GAMTN +F V + + LLVRVY
Sbjct: 15 DELRKILQALSTK-WGDVVEDFESLEVKPMKGAMTNEVFMVSWPRKETNLRCRKLLVRVY 73
Query: 139 GPNVESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQ 198
G VE +R+ E++ ++ GP+L G F GR+E+F+ ++ TL+ +D+RD S
Sbjct: 74 GEGVELFFNRDDEIRTFEYVARHGHGPTLLGRFAGGRVEEFI-HARTLSATDLRDPNISA 132
Query: 199 RIARRMKELHSGVPLLSKELKDEPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQ 258
+A +++ HS + + + W R+ W + ++ + N++ ++ + D+
Sbjct: 133 LVASKLRRFHS-IHIPGDRIM---LIWDRMRTW---VGQAKNLCSNEHSTEFGLDDIEDE 185
Query: 259 FLSIVAKYKKWLQQKPSYRKLVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXX 318
+ Q+ + +++ FCHND QYGN++
Sbjct: 186 INLLE-------QEVNNEQEIGFCHNDLQYGNIM-------------------------- 212
Query: 319 XXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNC 378
++E N + +ID+EY NP A+D+ANH E DY+
Sbjct: 213 ------------IDEETN----------AITIIDYEYASYNPIAYDIANHFCEMAADYHS 250
Query: 379 STPYVVFHDRFPTHEEMLNFIYSYLSHLNPKRKHMEEEVCELYNDILDWRGSVSLHWALW 438
+TP+++ + +P EE FI +YL+ + + EE++ +L +DI + + L W LW
Sbjct: 251 NTPHILDYTLYPGEEERRRFICNYLTSSGEEAR--EEDIEQLLDDIEKYTLASHLFWGLW 308
Query: 439 GIIQSGELTTEIDESEKTEE 458
GII E D E + +
Sbjct: 309 GIISGYVNKIEFDYIEYSRQ 328
>At1g71697 [M] KOG2686 Choline kinase
Length = 346
Score = 119 bits (297), Expect = 2e-26
Identities = 94/376 (25%), Positives = 160/376 (42%), Gaps = 71/376 (18%)
Query: 85 EDFKNLIIALRVKHWYKHDLDASMLRFTRISGAMTNAIFKVEYDNLPS------LLVRVY 138
ED K ++ L W D L + GAMTN ++++ + L +LVR+Y
Sbjct: 17 EDLKRVLQTLG-SSWGDVVEDLERLEVVPLKGAMTNEVYQINWPTLNGEDVHRKVLVRIY 75
Query: 139 GPNVESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQ 198
G V+ +R E++ +S GP L G F +GR+E+F+ ++ TL+ D+R +TS
Sbjct: 76 GDGVDLFFNRGDEIKTFECMSHHGYGPKLLGRFSDGRLEEFI-HARTLSADDLRVAETSD 134
Query: 199 RIARRMKELHSGVPLLSKELKDEPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQ 258
IA +++E H L W+R+ W L ++ + ++KY + ++
Sbjct: 135 FIAAKLREFHK----LDMPGPKNVLLWERLRTW---LKEAKNLASPIEMDKYRLEGLENE 187
Query: 259 FLSIVAKYKKWLQQKPSYRKLVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXX 318
+ + + +++ FCHND QYGN++
Sbjct: 188 INLLEERLTR------DDQEIGFCHNDLQYGNVM-------------------------- 215
Query: 319 XXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNC 378
++E+ N + +ID+EY NP A+D+ANH E +Y+
Sbjct: 216 ------------IDEVTN----------AITIIDYEYSSFNPIAYDIANHFCEMAANYHS 253
Query: 379 STPYVVFHDRFPTHEEMLNFIYSYLSHLNPKRKHMEEEVCELYNDILDWRGSVSLHWALW 438
TP+V+ + +P E FI +YL + ++EV L D + + + W LW
Sbjct: 254 DTPHVLDYTLYPGEGERRRFISTYLG--STGNATSDKEVERLLKDAESYTLANHIFWGLW 311
Query: 439 GIIQSGELTTEIDESE 454
GII E D E
Sbjct: 312 GIISGHVNKIEFDYME 327
>At2g26830 [I] KOG4720 Ethanolamine kinase
Length = 374
Score = 116 bits (290), Expect = 1e-25
Identities = 105/383 (27%), Positives = 168/383 (43%), Gaps = 76/383 (19%)
Query: 67 VPYVKSTLDISLPHDLLQEDFKNLIIALRVKHWYKHDLDASMLRFTRISGAMTNAIFKV- 125
+PY +D SLP L+ L L K+W +LD S+ R+SG +TN + KV
Sbjct: 23 IPYSSFVVDTSLPLPLMIPRIIELCKDL-FKNW--GELDDSLFSVERVSGGITNLLLKVS 79
Query: 126 ---EYDNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLEN 182
+ + S+ VR+YGPN E VI+RE E+ + LS G L G F NG ++ F+ N
Sbjct: 80 VKEDTNKEVSVTVRLYGPNTEYVINREREILAIKYLSAAGFGAKLLGGFGNGMVQSFI-N 138
Query: 183 SHTLTKSDIRDWKTSQRIARRMKELHSGVPLLSKELKDEPATWKRIEKWTNTLAKSSWIQ 242
+ TL SD+R+ K + +IAR + + H SK EP W I K+ + ++
Sbjct: 139 ARTLEPSDMREPKIAAQIARELGKFHKVDIPGSK----EPQLWVDILKFYEKASTLTF-- 192
Query: 243 DNDNINKYLMVSSWDQFLSIVAKYKKW--LQQKPSYRKLVFCHNDAQYGNLLFAAPVIPV 300
+ + K S+++ + + +++ L P +VF HND GN +
Sbjct: 193 EEPDKQKLFETISFEELHKEIIELREFTGLLNAP----VVFAHNDLLSGNFML------- 241
Query: 301 TADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPNP 360
++ KL +IDFEYG N
Sbjct: 242 -----------------------------------------NDEEEKLYLIDFEYGSYNY 260
Query: 361 PAFDLANHLSEWMHDYNCSTPYVVFHDRFPTHEEMLNFIYSYLSHLNPKRKHMEEEVCEL 420
FD+ NH +E+ Y+C + +P+ EE +FI YL P + EV +
Sbjct: 261 RGFDIGNHFNEYA-GYDCD------YSLYPSKEEQYHFIKHYLQPDKPDEVSI-AEVESV 312
Query: 421 YNDILDWRGSVSLHWALWGIIQS 443
+ + ++ + L+WA+W IIQ+
Sbjct: 313 FVETDAYKLASHLYWAIWAIIQA 335
>Hs6978649 [M] KOG2686 Choline kinase
Length = 395
Score = 108 bits (271), Expect = 2e-23
Identities = 100/355 (28%), Positives = 152/355 (42%), Gaps = 70/355 (19%)
Query: 109 LRFTRISGAMTNAIFKVEY-DNLPSL-------LVRVYGPNVESVIDREYELQVLARLSI 160
LR +SG ++N +F+ D+LPS+ L+R+YG ++ V E + A L+
Sbjct: 69 LRVYPVSGGLSNLLFRCSLPDHLPSVGEEPREVLLRLYGAILQGVDSLVLESVMFAILAE 128
Query: 161 QHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHSGVPLLSKELKD 220
+ +GP LYG F GR+EQ++ S L ++R+ S IA +M + H +KE
Sbjct: 129 RSLGPQLYGVFPEGRLEQYIP-SRPLKTQELREPVLSAAIATKMAQFHGMEMPFTKEPHW 187
Query: 221 EPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPSYRKLV 280
T +R K L + +N M S D+ + +K L+ PS +V
Sbjct: 188 LFGTMERYLKQIQDLPPTGL----PEMNLLEMYSLKDE----MGNLRKLLESTPS--PVV 237
Query: 281 FCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQ 340
FCHND Q GN+L + P AD
Sbjct: 238 FCHNDIQEGNILLLSE--PENAD------------------------------------- 258
Query: 341 EQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCS--TPYVVFHDRFPTHEEMLNF 398
L+++DFEY N FD+ NH EW++DY Y +PT E+ L+F
Sbjct: 259 ------SLMLVDFEYSSYNYRGFDIGNHFCEWVYDYTHEEWPFYKARPTDYPTQEQQLHF 312
Query: 399 IYSYLSHLNPKRKHMEEEVCELYNDIL--DWRGSVSLH--WALWGIIQSGELTTE 449
I YL+ +EE +L D+L R +++ H W LW I+Q+ T E
Sbjct: 313 IRHYLAEAKKGETLSQEEQRKLEEDLLVEVSRYALASHFFWGLWSILQASMSTIE 367
>CE27113 [M] KOG2686 Choline kinase
Length = 429
Score = 108 bits (269), Expect = 3e-23
Identities = 97/349 (27%), Positives = 159/349 (44%), Gaps = 45/349 (12%)
Query: 109 LRFTRISGAMTNAIFKVEYD-------NLPS-LLVRVY-GPNVESVIDREYELQVLARLS 159
LR +RI G M+N +F N P+ +L+RVY P ES + E + L LS
Sbjct: 76 LRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTL--LS 133
Query: 160 IQHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHS-GVPLLSKEL 218
+H+GP LYG F GR+E+++ S L+ +I S +IA+R+ ++H VP+
Sbjct: 134 ERHLGPKLYGIFSGGRLEEYIP-SRPLSCHEISLAHMSTKIAKRVAKVHQLEVPI----W 188
Query: 219 KDEPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPSYRK 278
K+ + +++W L + + ++ + VSS + +A+ ++L+ S K
Sbjct: 189 KEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVN--CLDLARELEFLRAHISLSK 246
Query: 279 --LVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIIN 336
+ FCHND Q GN+L +P + N
Sbjct: 247 SPVTFCHNDLQEGNIL-----LPKASSGNIRMPSLSDETQALGNSLSA----------FN 291
Query: 337 PSIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTP--YVVFHDRFPTHEE 394
P+ D +LV+IDFEY N AFD ANH EW DY+ Y + + FP +++
Sbjct: 292 PA------DPRLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQ 345
Query: 395 MLNFIYSYLSHL-NPKRKHMEEEVCELYNDILDWRGSVSLHWALWGIIQ 442
ML F +YL N + + ++ +L + L + W +WG++Q
Sbjct: 346 MLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQ 394
>7302122 [M] KOG2686 Choline kinase
Length = 495
Score = 94.7 bits (234), Expect = 3e-19
Identities = 82/324 (25%), Positives = 144/324 (44%), Gaps = 27/324 (8%)
Query: 133 LLVRVYGPN-----VESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLENSHTLT 187
+L+R+YG +ES+I E V A LS ++ GP L+G F GRIEQ++ + LT
Sbjct: 153 VLLRIYGQTHGDHALESMIT---ESVVFALLSERNYGPKLHGIFPGGRIEQYIP-ARALT 208
Query: 188 KSDIRDWKTSQRIARRMKELHS-GVPLLSKELKDEPATWKRIEKWTNTLAK--SSWIQDN 244
+++ + + +R+A +M E+HS +P+ K+ W +++W + L + +Q N
Sbjct: 209 TAELGEQRILKRVAEKMGEIHSLNIPM----SKEPDWIWNCMQRWVSGLESIVNGSVQTN 264
Query: 245 DN---INKYLMVSSWDQFLSIVAKYKKWLQQKPSYRKLVFCHNDAQYGNLLFAAPVIPVT 301
+ K + + ++ +A + + + +VFCHND Q GN+L P
Sbjct: 265 QKSSVLKKQMELMRTFDYVQEMAWIRSIIDE--GDYPVVFCHNDLQEGNILMRQPSAGQN 322
Query: 302 ADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPNPP 361
+ + + + D L++IDFEY N
Sbjct: 323 ERTPRESISSLSKSRSVSPSPCPELDTTNDSALDSSFMADNEPD--LIIIDFEYCAYNYR 380
Query: 362 AFDLANHLSEWMHDY-NCSTPYVVFH-DRFPTHEEMLNFIYSYLSHLNPKRKH--MEEEV 417
+DLANH EW DY N PY + T ++ +FI +YL + + +E+
Sbjct: 381 GYDLANHFIEWTFDYTNPQFPYFYHNSSNCATVQQRRDFIVNYLKKFHDDENYNITGQEL 440
Query: 418 CELYNDILDWRGSVSLHWALWGII 441
++ +I + L W+LW +I
Sbjct: 441 MKVDAEIQFFTMLSHLFWSLWSVI 464
>CE07553 [I] KOG4720 Ethanolamine kinase
Length = 342
Score = 91.7 bits (226), Expect = 3e-18
Identities = 83/343 (24%), Positives = 136/343 (39%), Gaps = 66/343 (19%)
Query: 109 LRFTRISGAMTNAIFKVEYDNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSLY 168
+ F S +TN IF + ++ RV+G N VIDRE E+ +L+ LY
Sbjct: 41 ITFEYFSVGITNKIFSAGFGT-EHVIFRVFGHNTNKVIDRENEVIAWKQLAEYGFAAPLY 99
Query: 169 GCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHSGVPLLSKELKDEPATWKRI 228
G F NG I FLE +L +RD K + IA+R+ +LHS VP K ++++
Sbjct: 100 GKFNNGLICGFLEGK-SLAIEQMRDSKFNMNIAKRIAQLHSSVPTNGK-----TPVFEKM 153
Query: 229 EKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPSYRKLVFCHNDAQY 288
+ L S + N + +S + K L++ +VFCHND
Sbjct: 154 RTFLQQLNPSFEKESQQNFFHENFPTDLGAEISKIEKMIVMLKE-----PIVFCHNDLLV 208
Query: 289 GNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQDAKL 348
N+++ ++ +
Sbjct: 209 HNIVY------------------------------------------------DSEKKSI 220
Query: 349 VVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTPYVVFHDRFPTHEEMLNFIYSYLSHLNP 408
ID+EY PN +D+ANH E+ S Y + T +E FI YL N
Sbjct: 221 EFIDYEYAFPNYALYDIANHFCEYA-GVEGSPDY----SKCLTKDEKWAFINDYLRFSNG 275
Query: 409 KRKHMEEEVCELYNDILDWRGSVSLHWALWGIIQSGELTTEID 451
K +H + + ++ ++L + + L WA+W ++Q+ T + D
Sbjct: 276 K-EHSDTRIATMFKNLLLFEAAAHLFWAVWALVQAQNSTIDFD 317
>At1g34100_2 [M] KOG2686 Choline kinase
Length = 267
Score = 91.3 bits (225), Expect = 3e-18
Identities = 78/314 (24%), Positives = 131/314 (40%), Gaps = 73/314 (23%)
Query: 145 VIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRM 204
+ +R+ E++ +S GP L G F GRIE+F+ N+ TL+ +++RD + R+A ++
Sbjct: 3 LFNRKDEIRTFEVVSRYGHGPKLLGRFSGGRIEEFI-NARTLSAANLRDAEVPTRVAAKL 61
Query: 205 KELHSGVPLLSKELKDEPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVA 264
+E H G+ + W R+ W AKS ++ L D+ +
Sbjct: 62 REFH-GINIPGDR---NVLIWDRMRNWLRQ-AKSLCTPED------LAEFGLDKIEDEIY 110
Query: 265 KYKKWLQQKPSYRKLVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXX 324
+ LQ K +++ FCHND QYGN++
Sbjct: 111 LLEHELQDKCKQQEIGFCHNDLQYGNIM-------------------------------- 138
Query: 325 XXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTPYVV 384
++E N + +ID+EY NP A+D+ANH E +Y
Sbjct: 139 ------IDEDTN----------AITIIDYEYASYNPVAYDIANHFCEMAANY-------- 174
Query: 385 FHDRFPTHEEMLNFIYSYLSHLNPKRKHMEEEVCELYNDILDWRGSVSLHWALWGIIQSG 444
+ EE FI++YLS + K E+++ +L D + + L W LWGII
Sbjct: 175 ---KVAGEEERRRFIHNYLSSSGEEPK--EDDIKQLLKDAEKYTLASHLFWGLWGIISGY 229
Query: 445 ELTTEIDESEKTEE 458
+ D +E + +
Sbjct: 230 VNKIDFDYAEYSRQ 243
>Hs4557455 [M] KOG2686 Choline kinase
Length = 456
Score = 89.0 bits (219), Expect = 2e-17
Identities = 81/303 (26%), Positives = 132/303 (42%), Gaps = 64/303 (21%)
Query: 151 ELQVLARLSIQHIGPSLYGCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHSG 210
E + A L+ + +GP LYG F GR+EQF+ S L ++ S IA +M H
Sbjct: 179 ESVMFAILAERSLGPKLYGIFPQGRLEQFIP-SRRLDTEELSLPDISAEIAEKMATFHGM 237
Query: 211 VPLLSKELKDEPATWKRIEKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWL 270
+KE K T +EK+ + + + +++ I K + S++ L + + L
Sbjct: 238 KMPFNKEPKWLFGT---MEKYLKEVLRIKFTEES-RIKKLHKLLSYNLPLEL-ENLRSLL 292
Query: 271 QQKPSYRKLVFCHNDAQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXX 330
+ PS +VFCHND Q GN+L
Sbjct: 293 ESTPS--PVVFCHNDCQEGNILLLEG---------------------------------- 316
Query: 331 LEEIINPSIQEQAQDAKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTPYVVFH---D 387
+E ++ KL++IDFEY N FD+ NH EWM+DY+ Y F
Sbjct: 317 ---------RENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYE-KYPFFRANIR 366
Query: 388 RFPTHEEMLNFIYSYL-------SHLNPKRKHMEEEVCELYNDILDWRGSVSLHWALWGI 440
++PT ++ L+FI SYL +L+ + K + +E E+ ++ + + W LW I
Sbjct: 367 KYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKE--EMLLEVNRFALASHFLWGLWSI 424
Query: 441 IQS 443
+Q+
Sbjct: 425 VQA 427
>7293191 [I] KOG4720 Ethanolamine kinase
Length = 495
Score = 77.0 bits (188), Expect = 7e-14
Identities = 76/332 (22%), Positives = 134/332 (39%), Gaps = 67/332 (20%)
Query: 125 VEY-DNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSLYGCFENGRIEQFLENS 183
V+Y DN+ +LVR+YG + +IDR+ E Q L + PSLY F+NG + +++ +
Sbjct: 197 VQYSDNV--VLVRIYGNKTDLLIDRKAETQNFLLLHTYGLAPSLYATFKNGLVYEYVPGT 254
Query: 184 HTLTKSDIRDWKTSQRIARRMKELHSGVPLL--SKELKDEPATWKRIEKWTNTL-AKSSW 240
TL + + +ARRM E+H V S K P WK+ + + + + + S
Sbjct: 255 -TLNTDSVLCPEIWPLVARRMAEMHRKVRKHGDSSATKPMPMIWKKTQSFLDLVPERFSD 313
Query: 241 IQDNDNINK-YLMVSSWDQFLSIVAKYKKWLQQKPSYRKLVFCHNDAQYGNLLFAAPVIP 299
+ + + + +L + + + + +Y + L +VF HND GN+++ +
Sbjct: 314 AEKHKRVKETFLPIGRLREEFNKLYEYLEALDS-----PIVFSHNDLLLGNVIYTQSLNT 368
Query: 300 VTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQDAKLVVIDFEYGGPN 359
V ID+EY N
Sbjct: 369 VN------------------------------------------------FIDYEYADYN 380
Query: 360 PPAFDLANHLSEWMHDYNCSTPYVVFHDRFPTHEEMLNFIYSYLSHLNPKRKHMEEEVCE 419
AFD+ NH +E C V + R+P E L ++ YL + +EV
Sbjct: 381 FQAFDIGNHFAEM-----CGVDEVDY-SRYPKREFQLQWLRVYLEEYLQRSNIQNDEVEL 434
Query: 420 LYNDILDWRGSVSLHWALWGIIQSGELTTEID 451
LY + + + + W +W ++Q+ T + D
Sbjct: 435 LYVQVNQFALASHIFWTVWSLLQAEHSTIDFD 466
>Hs8922650 [I] KOG4720 Ethanolamine kinase
Length = 394
Score = 65.9 bits (159), Expect = 2e-10
Identities = 86/358 (24%), Positives = 145/358 (40%), Gaps = 88/358 (24%)
Query: 50 KEGPATPQSLVLGESISVPYVKSTLDISLPHDLLQEDFKNLIIALRVKHWYKHDLDASML 109
+E P P++ +V Y ++D P D+L + LI LR HW +
Sbjct: 40 REPPGPPRAA------AVAYFGISVD---PDDILPGALR-LIQELR-PHW-----KPEQV 83
Query: 110 RFTRISGAMTNAIFK--VEYDNLPSLLVRVYGPNVESVIDREYELQVLARLSIQHIGPSL 167
R R + +TN + VE D +LVRVYG E ++DRE E++ L P L
Sbjct: 84 RTKRFTDGITNKLVACYVEEDMQDCVLVRVYGERTELLVDRENEVRNFQLLRAHSCAPKL 143
Query: 168 YGCFENGRIEQFLENSHTLTKSDIRDWKTSQRIARRMKELHSGVPLLSKELKDEPATWKR 227
Y F+NG ++++ L IR+ + + IA M ++H+ + + +P W +
Sbjct: 144 YCTFQNGLCYEYMQGV-ALEPEHIREPRLFRLIALEMAKIHT---IHANGSLPKPILWHK 199
Query: 228 IEKWTNTLAKSSWIQDNDNINKYLMVSSWDQFLSIVAKYKKWLQQKPSYRK--LVFCHND 285
+ + TL K + IN L S+ + ++ + WL++ S + +VFCHND
Sbjct: 200 MHNYF-TLVK-------NEINPSL--SADVPKVEVLERELAWLKEHLSQLESPVVFCHND 249
Query: 286 AQYGNLLFAAPVIPVTADXXXXXXXXXXXXXXXXXXXXXXXXXXXLEEIINPSIQEQAQD 345
N+++ SI+ +
Sbjct: 250 LLCKNIIY-------------------------------------------DSIKGHVR- 265
Query: 346 AKLVVIDFEYGGPNPPAFDLANHLSEWMHDYNCSTPYVVFHDRFPTHEEMLNFIYSYL 403
ID+EY G N AFD+ NH +E+ + V + +P E L +++ YL
Sbjct: 266 ----FIDYEYAGYNYQAFDIGNHFNEF------AGVNEVDYCLYPARETQLQWLHYYL 313
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,320,188
Number of Sequences: 60738
Number of extensions: 1411443
Number of successful extensions: 3825
Number of sequences better than 1.0e-05: 15
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 3755
Number of HSP's gapped (non-prelim): 29
length of query: 537
length of database: 30,389,216
effective HSP length: 111
effective length of query: 426
effective length of database: 23,647,298
effective search space: 10073748948
effective search space used: 10073748948
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)