ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4162 good S KOG4018 Function unknown Uncharacterized conserved protein, contains RWD domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4162 1412390 1413190 267
(267 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR152w [S] KOG4018 Uncharacterized conserved protein contains R... 229 4e-60
Hs20551750 [S] KOG4018 Uncharacterized conserved protein contain... 80 4e-15
CE24015 [S] KOG4018 Uncharacterized conserved protein contains R... 76 4e-14
Hs7706270 [S] KOG4018 Uncharacterized conserved protein contains... 76 6e-14
7301119 [S] KOG4018 Uncharacterized conserved protein contains R... 70 4e-12
At1g51730 [S] KOG4018 Uncharacterized conserved protein contains... 66 6e-11
>YDR152w [S] KOG4018 Uncharacterized conserved protein contains RWD domain
Length = 265
Score = 229 bits (583), Expect = 4e-60
Identities = 119/267 (44%), Positives = 164/267 (60%), Gaps = 10/267 (3%)
Query: 1 MDYKEEQTQELEILESIYPDEFTVLNSEYPEIELQIDIKLDPVPLDDSSYTVDSITNDHI 60
MDYKEEQ QELE+LESIYPDE ++N EYP+I+ ++ IKL+ D+ + +T +H
Sbjct: 1 MDYKEEQKQELEVLESIYPDELRIINDEYPKIKFEVAIKLEL----DTGDSTSVLTKEHT 56
Query: 61 LHTIFTLPENYPDEAPLIKIMPEEVPKFXXXXXXXXXXXXXXXXXXXXXXHGNPIVSKFE 120
+ F LPENYPDE LI + +EV HGN ++ KFE
Sbjct: 57 IIAEFKLPENYPDEPCLISLEAQEV-----ALNDNEEDNEEDEDEVEYDDHGNKVLKKFE 111
Query: 121 NIPDKIHFDEFIPQCIEKLNEQIQEDMLLGMQMCFALISNIKDYAEQWFQEKLSXXXXXX 180
N+PD I F ++P+ +L QI+ DMLLGMQMCFALIS+IK+ EQW+ E+L+
Sbjct: 112 NLPDLISFKGYLPELTVQLESQIETDMLLGMQMCFALISSIKERCEQWYSEQLNKLEKQY 171
Query: 181 XXXXXXXXXXXXXXFRGTKVTRESYIEWRAKFRKELGLDERDAQRRLAAHQGRLSGRQIF 240
F GTKVTRESY+EWR+KFR+EL LDERD RR+ AH G+L+G+Q+F
Sbjct: 172 ELEAQEREKKEQAKFHGTKVTRESYLEWRSKFRQELKLDERDQVRRMKAHHGKLTGKQMF 231
Query: 241 EQGLAG-DEDLDEENESLISEGVKAIA 266
EQG+ G ++ EE+++ + + K +A
Sbjct: 232 EQGVVGTGDEYMEEDDASVDDVAKGLA 258
>Hs20551750 [S] KOG4018 Uncharacterized conserved protein contains RWD domain
Length = 243
Score = 79.7 bits (195), Expect = 4e-15
Identities = 70/269 (26%), Positives = 109/269 (40%), Gaps = 64/269 (23%)
Query: 2 DYKEEQTQELEILESIYPDEFTVLNSEYPEIELQIDIKLDPVPLDDSSYTVDSITNDHIL 61
DY EEQ ELE LESIYPD FTVL+ P + + T ++ ND +
Sbjct: 3 DYGEEQRNELEALESIYPDSFTVLSENPPSFTITV--------------TSEAGENDETV 48
Query: 62 HTI--FTLPENYPDEAPLIKIMPEEVPKFXXXXXXXXXXXXXXXXXXXXXXHGNPIVSKF 119
T FT E YPDEAPL +I +
Sbjct: 49 QTTLKFTYSEKYPDEAPLYEIFSQ------------------------------------ 72
Query: 120 ENIPDKIHFDEFIPQCIEKLNEQIQEDMLLGMQMCFALISNIKDYAEQWFQEKLSXXXXX 179
EN+ D + ++ L Q +E+ LGM M F L++ +++ + + +
Sbjct: 73 ENLE-----DNDVSDILKLLALQAEEN--LGMVMIFTLVTAVQEKLNEIVDQIKTRREEE 125
Query: 180 XXXXXXXXXXXXXXXFRGTKVTRESYIEWRAKFRKE-LGLDERDAQRRLAAHQGRLSGRQ 238
F GT VT E+++ W+AKF E L + ++ + A + +LSG+Q
Sbjct: 126 KKQKEKEAEEAEKQLFHGTPVTIENFLNWKAKFDAELLEIKKKRMKEEEQAGKNKLSGKQ 185
Query: 239 IFEQGLAGDEDLDEENESLISEGVKAIAV 267
+FE D +LD + + + + V
Sbjct: 186 LFET----DHNLDTSDIQFLEDAGNNVEV 210
>CE24015 [S] KOG4018 Uncharacterized conserved protein contains RWD domain
Length = 240
Score = 76.3 bits (186), Expect = 4e-14
Identities = 74/268 (27%), Positives = 112/268 (41%), Gaps = 61/268 (22%)
Query: 1 MDYKEEQTQELEILESIYPDE-FTVLNSEYPEIELQIDIKLDPVPLDDSSYTVDSITNDH 59
M ++E+Q QE+E LE+IY +E V + +YP IEL I +K S+ D +D
Sbjct: 1 MSHQEQQEQEIEALEAIYSEEEIHVASRDYPNIELSIQLK--------SNQYEDPTDDDF 52
Query: 60 ILHTIFTLPENYPDEAPLIKIMPEEVPKFXXXXXXXXXXXXXXXXXXXXXXHGNPIVSKF 119
+ ENYPDE P+I + N I F
Sbjct: 53 DVELGIEFTENYPDEIPIITL--------------------------------NGIEDAF 80
Query: 120 ENIPDKIHFDEFIPQCIEKLNEQIQEDMLLGMQMCFALISNIKDYAEQWFQEKLSXXXXX 179
E I + I+KL +E+ LGM M FA++S ++D + K
Sbjct: 81 TA--------ERIAESIDKLRSVAEEN--LGMVMVFAIVSALQDEIGELVDVKKRAKEEK 130
Query: 180 XXXXXXXXXXXXXXXFRGTKVTRESYIEWRAKFRKELGLDERDAQR-RLAAHQGRLSGRQ 238
F GT VT +++ W+ +F +E + A++ RLA+ GRL+GRQ
Sbjct: 131 VEIEKEKKEAESRKKFEGTVVTPDTFRAWKDRFDEERKAEIDAAEKERLASLAGRLTGRQ 190
Query: 239 IF---------EQGLAGDEDLDEENESL 257
+F + L G +D E +ESL
Sbjct: 191 LFLRDATLNLSDVTLIGAQDEVEIDESL 218
>Hs7706270 [S] KOG4018 Uncharacterized conserved protein contains RWD domain
Length = 241
Score = 75.9 bits (185), Expect = 6e-14
Identities = 73/270 (27%), Positives = 110/270 (40%), Gaps = 68/270 (25%)
Query: 2 DYKEEQTQELEILESIYPDEFTVLNSEYPEIELQIDIKLDPVPLDDSSYTVDSITNDHIL 61
DY EEQ ELE LESIYPD FTVL+ P + + T ++ ND +
Sbjct: 3 DYGEEQRNELEALESIYPDSFTVLSENPPSFTITV--------------TSEAGENDETV 48
Query: 62 HTI--FTLPENYPDEAPLIKIMPEEVPKFXXXXXXXXXXXXXXXXXXXXXXHGNPIVSKF 119
T FT E YPDEAPL +I +
Sbjct: 49 QTTLKFTYSEKYPDEAPLYEIFSQ------------------------------------ 72
Query: 120 ENIPDKIHFDEFIPQCIEKLNEQIQEDMLLGMQMCFALISNIKDYAEQWF-QEKLSXXXX 178
EN+ D + ++ L Q +E+ LGM M F L++ +++ + Q KL
Sbjct: 73 ENLE-----DNDVSDILKLLALQAEEN--LGMVMIFTLVTAVQEKLNEIVDQIKLE---E 122
Query: 179 XXXXXXXXXXXXXXXXFRGTKVTRESYIEWRAKFRKE-LGLDERDAQRRLAAHQGRLSGR 237
F GT VT E+++ W+AKF E L + ++ + A + +LSG+
Sbjct: 123 RRKNKRKEAEEAEKQLFHGTPVTIENFLNWKAKFDAELLEIKKKRMKEEEQAGKNKLSGK 182
Query: 238 QIFEQGLAGDEDLDEENESLISEGVKAIAV 267
Q+FE D +LD + + + + V
Sbjct: 183 QLFET----DHNLDTSDIQFLEDAGNNVEV 208
>7301119 [S] KOG4018 Uncharacterized conserved protein contains RWD domain
Length = 244
Score = 69.7 bits (169), Expect = 4e-12
Identities = 57/239 (23%), Positives = 101/239 (41%), Gaps = 54/239 (22%)
Query: 2 DYKEEQTQELEILESIYPDEFTVLNSEYPEIELQIDIKLDPVPLDDSSYTVDSITNDHIL 61
+YKE+QT E+E L+SIY + +L +E P + QI P+ Y+ +
Sbjct: 4 NYKEDQTNEVEALDSIYCGDMEILATE-PHHKFQI-------PIATEEYSSEEPEKGLAC 55
Query: 62 HTIFTLPENYPDEAPLIKIMPEEVPKFXXXXXXXXXXXXXXXXXXXXXXHGNPIVSKFEN 121
+FT YPD AP+++I E FE
Sbjct: 56 KLVFTFTATYPDGAPVVEIEEPE---------------------------------NFE- 81
Query: 122 IPDKIHFDEFIPQCIEKLNEQIQEDMLLGMQMCFALISNIKDYAEQWFQEKLSXXXXXXX 181
D F + +E L + I+E+ LGM+M F+L+S+ +++ + + E
Sbjct: 82 -------DMFETRLLEHLQKTIEEN--LGMEMIFSLVSSAQEWLNERWDEHKFHQEELRE 132
Query: 182 XXXXXXXXXXXXXFRGTKVTRESYIEWRAKFRKELGLDERDAQRRLAAHQGRLSGRQIF 240
F GT+VT ES+++W+ +F + G+ + + ++ Q +GR++F
Sbjct: 133 QKLREIEEEERKKFEGTRVTVESFLKWKLEFEESTGIAAKREKNNVSKKQ---TGRELF 188
>At1g51730 [S] KOG4018 Uncharacterized conserved protein contains RWD domain
Length = 252
Score = 65.9 bits (159), Expect = 6e-11
Identities = 65/268 (24%), Positives = 104/268 (38%), Gaps = 62/268 (23%)
Query: 2 DYKEEQTQELEILESIYPDEFTVLNSEYPEIELQID-IKLDPVPLDDSSYTVDSITNDHI 60
+YK+EQ E+E LE+I DEF ++S + ++ P DD ++ + +
Sbjct: 3 EYKQEQEMEIEALEAILMDEFKEIHSSESGLNTSNRCFQITVTPQDDE---LEELAIPPV 59
Query: 61 -LHTIFTLPENYPDEAPLIKIMPEEVPKFXXXXXXXXXXXXXXXXXXXXXXHGNPIVSKF 119
L +F+ ENYPDEAPL+ +
Sbjct: 60 QLALVFSHTENYPDEAPLLDVKSIR----------------------------------- 84
Query: 120 ENIPDKIHFDEFIPQCIEKLNEQIQEDMLLGMQMCFALISNIKDYAEQWFQEKLSXXXXX 179
IH + + EKL ++ E+ LGM M + L+S+ KD W E
Sbjct: 85 -----GIHVSD-LTILKEKLEQEASEN--LGMAMIYTLVSSAKD----WLSEHYGQDDAA 132
Query: 180 XXXXXXXXXXXXXXXFRGTKVTRESYIEWRAKFRKELGLDERDAQRR---LAAHQGRLSG 236
G VT E+++ WR ++ EL L+ A + +L+G
Sbjct: 133 EFAEVEAAKEDEVIVPHGEPVTLETFLAWRERYEAELALERAKLMPESALTAPKEKKLTG 192
Query: 237 RQIFEQG-------LAGDEDLDEENESL 257
RQ FE G +A +ED +E+ E +
Sbjct: 193 RQWFESGRGRGTVVIADEEDEEEDEEDI 220
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.137 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,262,575
Number of Sequences: 60738
Number of extensions: 548847
Number of successful extensions: 1629
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1613
Number of HSP's gapped (non-prelim): 11
length of query: 267
length of database: 30,389,216
effective HSP length: 104
effective length of query: 163
effective length of database: 24,072,464
effective search space: 3923811632
effective search space used: 3923811632
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)