ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4202 good P KOG4505 Inorganic ion transport and metabolism Na+/H+ antiporter
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4202 1423833 1426535 901
(901 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR138w [P] KOG4505 Na+/H+ antiporter 799 0.0
SPAC3A11.09 [P] KOG4505 Na+/H+ antiporter 508 e-143
SPAC977.10 [P] KOG4505 Na+/H+ antiporter 352 1e-96
ECU04g1470 [P] KOG4505 Na+/H+ antiporter 197 5e-50
>YLR138w [P] KOG4505 Na+/H+ antiporter
Length = 985
Score = 799 bits (2063), Expect = 0.0
Identities = 465/968 (48%), Positives = 580/968 (59%), Gaps = 95/968 (9%)
Query: 2 VWSQLEVTNAHVAYAXXXXXXXXXXXXXXXXKERLYLGESSVAVLFGLIVGPHCLNWFNP 61
+W QLEV+ AHVAYA KE+LY+GES+VA +FGLIVGP CLNWFNP
Sbjct: 3 IWEQLEVSKAHVAYACVGVFSSIFSLVSLYVKEKLYIGESTVAGIFGLIVGPVCLNWFNP 62
Query: 62 TEWGNTDSITLEISRVVLCLQIFAVAVELPKKYMLKHWLSVTMLLVPVMTAGWLITGLFV 121
+WGN+DSITLEI+R+VLCLQIFAVAVELP+KYMLKHW+SVTMLL+PVMTAGWLI GLFV
Sbjct: 63 LKWGNSDSITLEITRIVLCLQIFAVAVELPRKYMLKHWVSVTMLLLPVMTAGWLIIGLFV 122
Query: 122 WILIPGLSFSDSLLVAACVTATDPILAQSVVSGKFAQRVPGHLRNLLSAESGCNDGMAFP 181
WILIPGL+FS SLL++AC+TATDPILAQSVVSGKFAQRVPGHLRNLLSAESGCNDGMAFP
Sbjct: 123 WILIPGLNFSASLLISACITATDPILAQSVVSGKFAQRVPGHLRNLLSAESGCNDGMAFP 182
Query: 182 FIYLSLNLIFYKRDAAEITKDWICITILWECLFGCILGSVIGYTGRRAIRFAEEKKLIDR 241
F++LS+NLI + + EI KDWIC+TIL+ECLFGC+LG IGY GR IRFAE+K +IDR
Sbjct: 183 FLFLSMNLILHPGNGREIVKDWICVTILYECLFGCLLGCFIGYVGRITIRFAEKKNIIDR 242
Query: 242 ESFLAFYVFLALMCAGFGSMLGVDDLLTSFAAGTAFSWDGWFXXXXXXXXXXXXIDLVLN 301
ESFLAFYV LA MCAGFGS+LGVDDLL SFAAG F+WDGWF IDL+LN
Sbjct: 243 ESFLAFYVVLAFMCAGFGSILGVDDLLVSFAAGATFAWDGWFSQKTQESNVSTVIDLLLN 302
Query: 302 LAYFVYFGAIIPWDQFNEASIGLSVWRPVLLAIAVIFLRRIPAVLTCKPVIPDIKSWREA 361
AYF+YFGAIIPW QFN IG +VWR ++L+I VIFLRRIPAV+ +P+IPDIKSWREA
Sbjct: 303 YAYFIYFGAIIPWSQFNNGEIGTNVWRLIILSIVVIFLRRIPAVMILRPLIPDIKSWREA 362
Query: 362 LFVGHFGPIGVGAIFAAILARSQLEAHVTGEETPLREIPPK-GSEYWQLMAVIWPLVCFL 420
LFVGHFGPIGVGAIFAAILAR +LE+ + E TPL +P K S++WQL+A IWP+ CF
Sbjct: 363 LFVGHFGPIGVGAIFAAILARGELESTFSDEPTPLNVVPSKEESKHWQLIACIWPITCFF 422
Query: 421 VLTSIIVHGSSVAVFTLGRHLNTITLTKSFTVHTTNGNGLTSKWMDRLPGLEKEGRSYSI 480
++TSIIVHGSSVA+ TLGRHLNTITLTK+FT HTTNG+ S WM RLP L+K GRS+S+
Sbjct: 423 IVTSIIVHGSSVAIITLGRHLNTITLTKTFTTHTTNGDNGKSSWMQRLPSLDKAGRSFSL 482
Query: 481 HRVDT-------------------MASDATKPLKKND---TVETSGIRAKPV-------- 510
HR+DT +A + ND +V TSGI A+P
Sbjct: 483 HRMDTQMTLSGDEGEAEEGGGRKGLAGGEDEEGLNNDQIGSVATSGIPARPAGGMPRRRK 542
Query: 511 -----------XXXXXXXXXXXXXXXXXXXNGAEEKNSLEIEREIRQKEAQAAAFALS-- 557
+E N L ER ++KEA+AA FALS
Sbjct: 543 LSRKEKRLNRRQKLRNKGREIFSSRSKNEMYDDDELNDLGRERLQKEKEARAATFALSTA 602
Query: 558 ----RDDESDLGAKSFTSLEDPNSLEKQQQTSLNSSEAGQEPPQRPVQSSGIT--PRPTF 611
R++E +G ED + EK+ + N++ + E +R G T PR +
Sbjct: 603 VNTQRNEEIGMGG---DEEEDEYTPEKEYSDNYNNTPS-FESSERSSSLRGRTYVPRNRY 658
Query: 612 ADEKIPFPSSQISGNXXXXXXXXXXXXXXXXXXXXXXFPLATDQANVAYQEGSRIILENR 671
E+ S+I + + AY +G+R+I+EN+
Sbjct: 659 DGEE---TESEIE-SEDEMENESERSMASSEERRIRKMKEEEMKPGTAYLDGNRMIIENK 714
Query: 672 KGEVVDEAQLVPREDVLND--------HAELSTTPTNHTINS---LKRVLTPTRF-RHST 719
+GE++++ + R + +D H+ L+TT TN + +S LKR+LTPT + +
Sbjct: 715 QGEILNQVDIEDRNEARDDEVSVDSTAHSSLTTTMTNLSSSSGGRLKRILTPTSLGKIHS 774
Query: 720 LVDTDTSK----KFYAYKYGTQLVIETDKGEIVRRYQINQHXXXXXXXXXXXXXRSNSVV 775
LVD K K++A+K L+IE + G++++RY+IN H R++SVV
Sbjct: 775 LVDKGKDKNKNSKYHAFKIDNLLIIENEDGDVIKRYKINPH--KSDDDKSKNRPRNDSVV 832
Query: 776 AKALSMVKLGKSSTSKQHPDSAQRPTSASSGSSLNSRQNFKNKVIARQLTPLP---VVDH 832
++AL+ V L + S P + A G S K R LTP P V D
Sbjct: 833 SRALTAVGLKSKANSGVPPPVDEE--KAIEGPSRKGPGMLKK----RTLTPAPPRGVQDS 886
Query: 833 LXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRARRLTALGISTA 892
L R RRL ALG TA
Sbjct: 887 L----------DLEDEPSSEEDLGDSYNMDDSEDYDDNAYESETEFERQRRLNALGEMTA 936
Query: 893 PRHQDDEE 900
P QDDEE
Sbjct: 937 PADQDDEE 944
>SPAC3A11.09 [P] KOG4505 Na+/H+ antiporter
Length = 759
Score = 508 bits (1308), Expect = e-143
Identities = 256/491 (52%), Positives = 337/491 (68%), Gaps = 12/491 (2%)
Query: 1 MVWSQLEVTNAHVAYAXXXXXXXXXXXXXXXXKERLYLGESSVAVLFGLIVGPHCLNWFN 60
M WSQ+E++ H+AYA KE+L+LGE+++A GLI GP+ F
Sbjct: 1 MAWSQVEISKPHLAYAIIGGFTSLFMLCSLIIKEKLFLGEATMATATGLIFGPYVAKLFV 60
Query: 61 PTEWGNTDSITLEISRVVLCLQIFAVAVELPKKYMLKHWLSVTMLLVPVMTAGWLITGLF 120
PT WGNTD IT E++RV+L +++FA ELP+ YML+HW S+ ++L+PVM GWL++ F
Sbjct: 61 PTSWGNTDYITEELARVLLVVEVFAAGAELPRAYMLRHWRSMFVMLLPVMIFGWLVSTGF 120
Query: 121 VWILIPGLSFSDSLLVAACVTATDPILAQSVVS-GKFAQRVPGHLRNLLSAESGCNDGMA 179
++ LIP LSF +SL +AAC+TATDP+LA S+V GKFA+RVPGHLRN+L AESGCNDGMA
Sbjct: 121 MYALIPRLSFLESLAIAACITATDPVLASSIVGKGKFARRVPGHLRNMLLAESGCNDGMA 180
Query: 180 FPFIYLSLNLIFYKRDAAEITKDWICITILWECLFGCILGSVIGYTGRRAIRFAEEKKLI 239
PF+YL++ LI K A +DW+CI IL+EC FGC+LG++IG R+ I+F+E + L+
Sbjct: 181 IPFLYLAIYLIIEK-PARHAGRDWVCIIILYECTFGCVLGAIIGVIARKMIKFSERRGLM 239
Query: 240 DRESFLAFYVFLALMCAGFGSMLGVDDLLTSFAAGTAFSWDGWFXXXXXXXXXXXXIDLV 299
DRESFL FY LAL C G G+++GVDDLL SF AG AFSWD WF IDL+
Sbjct: 240 DRESFLVFYFVLALFCGGIGTIIGVDDLLVSFCAGAAFSWDSWFSKKTEESHVSNVIDLL 299
Query: 300 LNLAYFVYFGAIIPWDQFNEASIGLSVWRPVLLAIAVIFLRRIPAVLTCKPVIPDIKSWR 359
LNL++FVY GAI+PW QF+ + LSVWR V+LAI ++ RRIPAVL K +PDI +WR
Sbjct: 300 LNLSFFVYVGAIMPWPQFHMPHMDLSVWRLVVLAICILIARRIPAVLLFKSFVPDIINWR 359
Query: 360 EALFVGHFGPIGVGAIFAAILARSQLEAHVTGEETPLREIPPKGSEYWQLMAVIWPLVCF 419
EALF GHFGPIGVGA++ ++AR++LE H T E P I W + V+WP+VCF
Sbjct: 360 EALFAGHFGPIGVGALYTCLVARAELEVHSTVPE-PNDAIENPEIPNWYCIQVMWPVVCF 418
Query: 420 LVLTSIIVHGSSVAVFTLGRHLNTITLTKSFTVHTTNGNGLTSKWMDRLPGLEKEGRSYS 479
LVL+SIIVHGSS+A F LG+ +NT+ L SFT S + LP + ++G+S
Sbjct: 419 LVLSSIIVHGSSIAFFMLGKRINTLAL--SFT------RTRDSHFAFNLPRV-RQGQSLP 469
Query: 480 IHRVDTMASDA 490
I RVD + S A
Sbjct: 470 IKRVDALRSSA 480
>SPAC977.10 [P] KOG4505 Na+/H+ antiporter
Length = 468
Score = 352 bits (904), Expect = 1e-96
Identities = 176/453 (38%), Positives = 273/453 (59%), Gaps = 8/453 (1%)
Query: 1 MVWSQLEVTNAHVAYAXXXXXXXXXXXXXXXXKERLYLGESSVAVLFGLIVGPHCLNWFN 60
M W QL++ H+A +++L +GE+ + + GLI GPH +
Sbjct: 1 MGWRQLDIDKVHLALIVAGGFITFFCYFSEVFRKKLLVGEAVLGSITGLIFGPHAAKLVD 60
Query: 61 PTEWGNT-DSITLEISRVVLCLQIFAVAVELPKKYMLKHWLSVTMLLVPVMTAGWLITGL 119
P WG+ D +T+EI R+VL +++FA A+ELP Y ++ S+ ++L+PVM GWL+T
Sbjct: 61 PFSWGDHGDYLTVEICRIVLDVRVFASAIELPGAYFQHNFRSIIVMLLPVMAYGWLVTAG 120
Query: 120 FVWILIPGLSFSDSLLVAACVTATDPILAQSVVS-GKFAQRVPGHLRNLLSAESGCNDGM 178
F + L P ++F SLL+A C+T+TDP+L+ +V G A++ P +R+LL AESGCNDGM
Sbjct: 121 FAYALFPQINFLGSLLIAGCITSTDPVLSALIVGEGPLAKKTPERIRSLLIAESGCNDGM 180
Query: 179 AFPFIYLSLNLIFYKRDAAEITKDWICITILWECLFGCILGSVIGYTGRRAIRFAEEKKL 238
A PF Y ++ L+ K + +DW+ + +L+EC FG G VIGY ++ A++ +L
Sbjct: 181 AVPFFYFAIKLLTVK-PSRNAGRDWVLLVVLYECAFGIFFGCVIGYLLSFILKHAQKYRL 239
Query: 239 IDRESFLAFYVFLALMCAGFGSMLGVDDLLTSFAAGTAFSWDGWFXXXXXXXXXXXXIDL 298
ID S+ + + + L+C+G G+++GVDDLL SF AG F+W+ F ID
Sbjct: 240 IDAISYYSLPLAIPLLCSGIGTIIGVDDLLMSFFAGILFNWNDLFSKNISACSVPAFIDQ 299
Query: 299 VLNLAYFVYFGAIIPWDQFNEASIGLSVWRPVLLAIAVIFLRRIPAVLTCKPVIPDIKSW 358
+L +F Y+G IIPW+ FN + GL VWR ++ +I + RR+P V + KP++PDIK+W
Sbjct: 300 TFSLLFFTYYGTIIPWNNFNWSVEGLPVWRLIVFSILTLVCRRLPVVFSVKPLVPDIKTW 359
Query: 359 REALFVGHFGPIGVGAIFAAILARSQLEAHVTGEETPLREIPPKGSEYWQLMAVIWPLVC 418
+EALFVGHFGPIGV A++ A LA+ L + + I + + L +IWP++
Sbjct: 360 KEALFVGHFGPIGVCAVYMAFLAKLLL-----SPDEIEKSIYESTTVFSTLNEIIWPIIS 414
Query: 419 FLVLTSIIVHGSSVAVFTLGRHLNTITLTKSFT 451
F++L+SIIVHG S+ V + L ++ L + T
Sbjct: 415 FVILSSIIVHGFSIHVLVIWGKLKSLYLNRKVT 447
>ECU04g1470 [P] KOG4505 Na+/H+ antiporter
Length = 414
Score = 197 bits (502), Expect = 5e-50
Identities = 118/349 (33%), Positives = 193/349 (54%), Gaps = 13/349 (3%)
Query: 33 KERLYLGESSVAVLFGLIVGPHCLNWFNPTEWGNTDSITLEISRVVLCLQIFAVAVELPK 92
K+ L L E V+VL G+++G H LN F+ T + + + SRVVLC+Q VA+ LPK
Sbjct: 26 KDSLLLSEPLVSVLTGIVLGHHVLNVFD-TNYVYSKIMMYNFSRVVLCIQTMTVAMSLPK 84
Query: 93 KYMLKHWLSVTMLLVPVMTAGWLITGLFVWILIPGLSFSDSLLVAACVTATDPILAQSVV 152
Y+L+ W S+ +L+ V + V+ L + + S +A+C+T TDPIL+ S++
Sbjct: 85 NYVLEKWQSIFVLVGMVGIIKCFFSFCLVY-LFTNYNKATSWSLASCLTPTDPILSSSII 143
Query: 153 SGKFAQR-VPGHLRNLLSAESGCNDGMAFPFIYLSLNLIFYKRDAAEITKDWICITILWE 211
GK A + VP +R LL+AESG NDGM ++L L+L+ + + + +L
Sbjct: 144 HGKLAMKMVPERIRMLLAAESGINDGMGIFLLFLGLDLL--RHGLRKGMYPFFIDNLLIR 201
Query: 212 CLFGCILGSVIGYTGRRAIRFAEEKKLIDRESFLAFYVFLALMCAGFGSMLGVDDLLTSF 271
++G VIG R+ +++ K+L ESFL V L + +G +L+ F
Sbjct: 202 VFVSALIGLVIGILSRQGLKYCYTKRLAGVESFLIHGVILTVFSMALMEFMGGSELICIF 261
Query: 272 AAGTAFSWDGWFXXXXXXXXXXXXIDLVLNLAYFVYFGAIIPWDQFNEASIGLSVWRPVL 331
AG FS+D WF D +L+ ++F++FG+ I + +F ++ V+
Sbjct: 262 FAGIVFSFDEWFVLETKNSRLQEITDTILSTSFFLFFGSRIDFSRFTLSN--------VI 313
Query: 332 LAIAVIFLRRIPAVLTCKPVIPDIKSWREALFVGHFGPIGVGAIFAAIL 380
+++ +IF+RR P IP+++S++EALF+G FGPIGVGA++ A+L
Sbjct: 314 ISLTIIFIRRPAICYLMYPHIPELRSFKEALFIGWFGPIGVGALYYALL 362
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.135 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,040,394
Number of Sequences: 60738
Number of extensions: 1713992
Number of successful extensions: 4988
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4962
Number of HSP's gapped (non-prelim): 8
length of query: 901
length of database: 30,389,216
effective HSP length: 115
effective length of query: 786
effective length of database: 23,404,346
effective search space: 18395815956
effective search space used: 18395815956
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)