ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4238 good E KOG0186 Amino acid transport and metabolism Proline oxidase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4238 1437692 1439092 467
(467 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR142w [E] KOG0186 Proline oxidase 545 e-155
SPCC70.03c [E] KOG0186 Proline oxidase 106 8e-23
HsM5174645 [E] KOG0186 Proline oxidase 89 1e-17
Hs19924111 [E] KOG0186 Proline oxidase 89 1e-17
CE15561 [E] KOG0186 Proline oxidase 87 7e-17
At3g30775 [E] KOG0186 Proline oxidase 84 3e-16
7295507 [E] KOG0186 Proline oxidase 78 2e-14
Hs10864043 [E] KOG0186 Proline oxidase 75 3e-13
At5g38710 [E] KOG0186 Proline oxidase 69 1e-11
>YLR142w [E] KOG0186 Proline oxidase
Length = 476
Score = 545 bits (1404), Expect = e-155
Identities = 275/438 (62%), Positives = 336/438 (75%), Gaps = 6/438 (1%)
Query: 31 SQTNATKSTMAPATAIHDMAASQHTVPAPDAHLRTLSKSELFSLGMIGVCTINKPMLDLV 90
+ +N + M A + S P L+TL K ELF LG IG+ T+N L+ +
Sbjct: 33 THSNTAANLMVETPAANANGNSVMAPPNSINFLQTLPKKELFQLGFIGIATLNSFFLNTI 92
Query: 91 IKLFPYVPLFMVKLFISPLYCGGDTPAEVVETGKRLQNRGINNMMLSLTIEDAEGTKNVD 150
IKLFPY+P+ ++K F+S LYCGG+ EV+E GKRLQ RGI+NMMLSLTIE++EGTK++
Sbjct: 93 IKLFPYIPIPVIKFFVSSLYCGGENFKEVIECGKRLQKRGISNMMLSLTIENSEGTKSLS 152
Query: 151 ---INYIVSETIKSVHTILKPLLVEQLESGKDINSIPPGYIALKPSALVANPAQVLLNFN 207
++ IV ETI SVH IL P ++ QLES K IN I PGYIALKPSALV NP +VL NF+
Sbjct: 153 STPVDQIVKETISSVHNILLPNIIGQLES-KPINDIAPGYIALKPSALVDNPHEVLYNFS 211
Query: 208 NPEWKTQRDELVNNCSKITQEVYNLNQELLAKYPERKSPFFVSTIDAEKYDLQKAGVYEL 267
NP +K QRD+L+ NCSKIT+E++ LNQ LL KYPERK+PF VSTIDAEKYDLQ+ GVYEL
Sbjct: 212 NPAYKAQRDQLIENCSKITKEIFELNQSLLKKYPERKAPFMVSTIDAEKYDLQENGVYEL 271
Query: 268 QRILFSKFNPASSPLISCVGTWQLYLVDSAADLAKDYERAKKEGYKLGLKLVRGAYIHSE 327
QRILF KFNP SS LISCVGTWQLYL DS + + + A++ GYKLGLKLVRGAYIHSE
Sbjct: 272 QRILFQKFNPTSSKLISCVGTWQLYLRDSGDHILHELKLAQENGYKLGLKLVRGAYIHSE 331
Query: 328 PNRS-VVYATKEGTDANYNKVMTTAIKDLLVNGEKSYYGHLVVASHNYDSQMLATKLL-A 385
NR+ +++ K GTD NY++++T + DL++NGE SY+GHLVVASHNY SQML T LL +
Sbjct: 332 KNRNQIIFGDKTGTDENYDRIITQVVNDLIINGEDSYFGHLVVASHNYQSQMLVTNLLKS 391
Query: 386 DQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVENIIKYVPWGPPLETKDYLLRRLQENG 445
Q NS K+N+ L QLLGM+DNVT+DLITNH +NIIKYVPWGPPLETKDYLLRRLQENG
Sbjct: 392 TQDNSYAKSNIVLGQLLGMADNVTYDLITNHGAKNIIKYVPWGPPLETKDYLLRRLQENG 451
Query: 446 DAVRADNGIPLIKAVLKT 463
DAVR+DNG PLIKA+ K+
Sbjct: 452 DAVRSDNGWPLIKAIAKS 469
>SPCC70.03c [E] KOG0186 Proline oxidase
Length = 492
Score = 106 bits (264), Expect = 8e-23
Identities = 103/379 (27%), Positives = 170/379 (44%), Gaps = 51/379 (13%)
Query: 110 YCGGDTPAEVVETGKRLQNRGINNMMLSLTIEDAEGTKNVDINYIVSETI---------K 160
+CGG+TP V+ T LQ GI + + D +G ++D+N I S+ + +
Sbjct: 106 FCGGETPQAVMATMDTLQAAGITSCLNYSREVDLDG--DMDVNKIASQGVVPPQVPVPSE 163
Query: 161 SVHTILKPLLVEQLESGK---DINSIPPGYIALKPSALVANPAQV-----LLNFNNPEWK 212
+L+ + + ES D+ + PG + NP + +LN E
Sbjct: 164 KNQKVLRQIADKAFESNMHIIDMATYKPGTVCAVKLTPFINPLVLQRYNSILNQYPVESA 223
Query: 213 TQRDELVNNCSKITQEVYNLNQ------ELLAKYPERKSPFFVSTIDAEKYDLQKAGVYE 266
E + + T EV L + +L E++ P F+ D A +
Sbjct: 224 CNYLEHLKSPELSTYEVSELKKFWEYADKLCQFAKEKQIPLFIDAEQTYFQDCMHAVTVD 283
Query: 267 LQRILFSKFNPASSPLISCVGTWQLYLVDSAADLAKDYERAKKEGYKLGLKLVRGAYIHS 326
L R K+N + + + T+QLYL S + ++ EG+ +G KLVRGAY++S
Sbjct: 284 LMR----KYNKEVAIVHN---TYQLYLKKSRKIMDDHIKKCVAEGWLMGAKLVRGAYLNS 336
Query: 327 EPNRSVVYATKEGTDANYNKVMTTAIKDLL-------------VNGEKSYYGHLVVASHN 373
EP R +++ TK TD +++ + I + K +G ++VASHN
Sbjct: 337 EP-RFLIHDTKAETDKDFDSAVEAIIAAAAKFAPGDPASASDPIASRKGKWG-IMVASHN 394
Query: 374 YDSQMLATKLLADQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVEN----IIKYVPWGP 429
+ + L +K K + LAQLLGM+D++T+ L + + + I+KYV GP
Sbjct: 395 KKTMFESVNLAETKKVDFTKTSFYLAQLLGMADDITYALAYSQRNQQPNFCIVKYVSCGP 454
Query: 430 PLETKDYLLRRLQENGDAV 448
E YL+RR +EN DA+
Sbjct: 455 ISEVLPYLVRRARENIDAL 473
>HsM5174645 [E] KOG0186 Proline oxidase
Length = 516
Score = 89.4 bits (220), Expect = 1e-17
Identities = 67/205 (32%), Positives = 102/205 (49%), Gaps = 17/205 (8%)
Query: 252 IDAEKYDLQKAGVYELQRILFSKFNPASSPLISCVGTWQLYLVDSAADLAKDYERAKKEG 311
+DAE+ Q A + L + KFN PLI T+Q YL D+ ++ D E A++EG
Sbjct: 295 VDAEQTYFQPA-ISRLTLEMQRKFN-VEKPLI--FNTYQCYLKDAYDNVTLDVELARREG 350
Query: 312 YKLGLKLVRGAYIHSEPNRSV-------VYATKEGTDANYNKVMTTAIKDLLVNGEKSYY 364
+ G KLVRGAY+ E R+ + T E T+A Y++ + +++L N +
Sbjct: 351 WCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAK---- 406
Query: 365 GHLVVASHNYDSQMLATKLLADQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVENIIKY 424
++VASHN D+ A + + + V QLLGM D ++ L + KY
Sbjct: 407 AKVMVASHNEDTVRFALRRMEELGLHPADHQVYFGQLLGMCDQISFPL--GQAGYPVYKY 464
Query: 425 VPWGPPLETKDYLLRRLQENGDAVR 449
VP+GP +E YL RR EN ++
Sbjct: 465 VPYGPVMEVLPYLSRRALENSSLMK 489
>Hs19924111 [E] KOG0186 Proline oxidase
Length = 600
Score = 89.4 bits (220), Expect = 1e-17
Identities = 67/205 (32%), Positives = 102/205 (49%), Gaps = 17/205 (8%)
Query: 252 IDAEKYDLQKAGVYELQRILFSKFNPASSPLISCVGTWQLYLVDSAADLAKDYERAKKEG 311
+DAE+ Q A + L + KFN PLI T+Q YL D+ ++ D E A++EG
Sbjct: 379 VDAEQTYFQPA-ISRLTLEMQRKFN-VEKPLI--FNTYQCYLKDAYDNVTLDVELARREG 434
Query: 312 YKLGLKLVRGAYIHSEPNRSV-------VYATKEGTDANYNKVMTTAIKDLLVNGEKSYY 364
+ G KLVRGAY+ E R+ + T E T+A Y++ + +++L N +
Sbjct: 435 WCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAK---- 490
Query: 365 GHLVVASHNYDSQMLATKLLADQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVENIIKY 424
++VASHN D+ A + + + V QLLGM D ++ L + KY
Sbjct: 491 AKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPL--GQAGYPVYKY 548
Query: 425 VPWGPPLETKDYLLRRLQENGDAVR 449
VP+GP +E YL RR EN ++
Sbjct: 549 VPYGPVMEVLPYLSRRALENSSLMK 573
>CE15561 [E] KOG0186 Proline oxidase
Length = 564
Score = 86.7 bits (213), Expect = 7e-17
Identities = 72/247 (29%), Positives = 120/247 (48%), Gaps = 20/247 (8%)
Query: 216 DELVNNCS-KITQEVYNLNQELL--AKYPERKSPFFVSTIDAEKYDLQKAGVYELQRILF 272
+ L+ N S + QE N+ + L A+Y K + +DAE+ LQ A + ++ +
Sbjct: 301 EPLIQNLSNEEEQEFRNMVRRTLDVAEYAIEKGVRIM--VDAEQTYLQPA-ISKITIEMM 357
Query: 273 SKFNPASSPLISCVGTWQLYLVDSAADLAKDYERAKKEGYKLGLKLVRGAYIHSEPNRSV 332
K+N + + T+Q YL + ++ D + A++EG+ G KLVRGAY+ E R+
Sbjct: 358 KKYNKGRGNIFN---TYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAK 414
Query: 333 VYATK-------EGTDANYNKVMTTAIKDLLVNGEKSYYGHLVVASHNYDSQMLATKLLA 385
+ E T Y +T ++ G+ + ++VASHN D+ A L+
Sbjct: 415 AIGYEDPINDNFEATSKMYESCLTRIADEVHRRGKTNV--SVMVASHNEDTVRFALNLMK 472
Query: 386 DQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVENIIKYVPWGPPLETKDYLLRRLQENG 445
++ S + + +AQL GM D V+ L ++ KY+P+GP E YL RR ENG
Sbjct: 473 EKCISPSERVMCMAQLYGMCDQVSFSL--GQAGFSVYKYLPYGPVEEVLPYLSRRALENG 530
Query: 446 DAVRADN 452
++ N
Sbjct: 531 SVLKKAN 537
>At3g30775 [E] KOG0186 Proline oxidase
Length = 499
Score = 84.3 bits (207), Expect = 3e-16
Identities = 75/218 (34%), Positives = 103/218 (46%), Gaps = 18/218 (8%)
Query: 234 QELLAKYPERKSPFFVSTIDAEKYDLQKAGVYELQRILFSKFNPASSPLISCVGTWQLYL 293
QE+ K E P IDAE LQ A Y P++ T Q YL
Sbjct: 266 QEICRKCQESNVPLL---IDAEDTILQPAIDYMAYSSAIMFNADKDRPIV--YNTIQAYL 320
Query: 294 VDSAADLAKDYERAKKEGYKLGLKLVRGAYIHSEPN-------RSVVYATKEGTDANYNK 346
D+ L + A+KE +G KLVRGAY+ SE + +S V+ T + T + YN
Sbjct: 321 RDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYND 380
Query: 347 VMTTAIKDLLVNGEKSYYGHLVVASHNYDSQMLATKLLADQKNSVGKANVTLAQLLGMSD 406
M T + + NG S +G +V+A+HN DS LA++ +D + AQL GMSD
Sbjct: 381 CM-TFLMEKASNG--SGFG-VVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSD 436
Query: 407 NVTHDLITNHKVENIIKYVPWGPPLETKDYLLRRLQEN 444
++ L N+ KY+P+GP YLLRR EN
Sbjct: 437 ALSFGL--KRAGFNVSKYMPFGPVATAIPYLLRRAYEN 472
>7295507 [E] KOG0186 Proline oxidase
Length = 671
Score = 78.2 bits (191), Expect = 2e-14
Identities = 75/280 (26%), Positives = 127/280 (44%), Gaps = 21/280 (7%)
Query: 179 DINSIPPGYIALKP-SALVANPAQVLLNFNNPEWKT-QRDELVNNCSKITQEVYNLNQEL 236
++ S G + L P S +V +Q+ F P+ +T Q L++ +E++
Sbjct: 361 NVTSDKEGILHLFPWSGIVDEDSQLSDTFRVPDPQTGQMRRLISQIPPKEEEMFRNMIRR 420
Query: 237 LAKYPERKSPFFVST-IDAEKYDLQKAGVYELQRILFSKFNPASSPLISCVGTWQLYLVD 295
L + + V +DAE+ Q A + RI + T+Q YL +
Sbjct: 421 LNTIVKAAADLDVRIMVDAEQTYFQPA----ISRITLEMMRKYNKDKAIVFNTYQCYLRE 476
Query: 296 SAADLAKDYERAKKEGYKLGLKLVRGAYIHSEPNRS-------VVYATKEGTDANYNKVM 348
+ ++ D E+AK++ + G KLVRGAY+ E +R+ V T E T Y++ +
Sbjct: 477 TFREVNTDLEQAKRQNFYFGAKLVRGAYMDQERDRAKSLGYPDPVNPTFEATTDMYHRTL 536
Query: 349 TTAIKDLLV----NGEKSYYGHLVVASHNYDSQMLATKLLADQKNSVGKANVTLAQLLGM 404
+ ++ + + + + G ++VASHN D+ A + + + S + QLLGM
Sbjct: 537 SECLRRIKLMKDCDDDARKIG-IMVASHNEDTVRFAIQQMKEIGISPEDKVICFGQLLGM 595
Query: 405 SDNVTHDLITNHKVENIIKYVPWGPPLETKDYLLRRLQEN 444
D +T L + KY+P+GP E YL RR QEN
Sbjct: 596 CDYITFPL--GQAGYSAYKYIPYGPVEEVLPYLSRRAQEN 633
>Hs10864043 [E] KOG0186 Proline oxidase
Length = 536
Score = 74.7 bits (182), Expect = 3e-13
Identities = 70/247 (28%), Positives = 112/247 (45%), Gaps = 37/247 (14%)
Query: 232 LNQELLAKYPERKSPFFVSTIDAEKYDLQKAGVYELQRIL---------------FSKFN 276
L+ E LA+ + VS ++AE+ +A + L R+ ++ N
Sbjct: 262 LSPERLAEAMDSGQNLQVSCLNAEQNQHLRASLSRLHRVAQYARAQHVRLLVDAEYTSLN 321
Query: 277 PASSPLISCVG---------------TWQLYLVDSAADLAKDYERAKKEGYKLGLKLVRG 321
PA S L++ + T+Q L D+ L +D E A + G G+KLVRG
Sbjct: 322 PALSLLVAALAVRWNSPGEGGPWVWNTYQACLKDTFERLGRDAEAAHRAGLAFGVKLVRG 381
Query: 322 AYIHSEPNRSVVYATKEGTDANYNKVMTTAIKDL-LVNGEKSYYG---HLVVASHNYDSQ 377
AY+ E + ++ ++ T +Y + + L L+ + +G HL+VASHN +S
Sbjct: 382 AYLDKERAVAQLHGMEDPTQPDYEATSQSYSRCLELMLTHVARHGPMCHLMVASHNEESV 441
Query: 378 MLATKLLADQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVENIIKYVPWGPPLETKDYL 437
ATK + + + V QLLGM D+V+ L + K +P+G E YL
Sbjct: 442 RQATKRMWELGIPL-DGTVCFGQLLGMCDHVS--LALGQAGYVVYKSIPYGSLEEVIPYL 498
Query: 438 LRRLQEN 444
+RR QEN
Sbjct: 499 IRRAQEN 505
>At5g38710 [E] KOG0186 Proline oxidase
Length = 476
Score = 68.9 bits (167), Expect = 1e-11
Identities = 62/203 (30%), Positives = 91/203 (44%), Gaps = 21/203 (10%)
Query: 252 IDAEKYDLQKAGVYELQRILFSKFNPASSPLISCVGTWQLYLVDSAADLAKDYERAKKEG 311
IDAE LQ A Y + P++ T Q YL D+ L ++K
Sbjct: 260 IDAEDTILQPAIDYMAYWSAIMFNSDKDRPIV--YNTIQAYLKDAGERLHLALRESEKMN 317
Query: 312 YKLGLKLVRGAYIHSEPN-------RSVVYATKEGTDANYNKVMTTAIKDLLVNGEKSYY 364
+G KLVRGAY+ SE +S V+ T + T YN M+ + EK+
Sbjct: 318 VPIGFKLVRGAYMSSEAKLADSLGYKSPVHDTIQNTHDCYNDCMSFLM-------EKASN 370
Query: 365 GH---LVVASHNYDSQMLATKLLADQKNSVGKANVTLAQLLGMSDNVTHDLITNHKVENI 421
G +++A+HN DS L + ++ + + AQL GMSD ++ L N+
Sbjct: 371 GSGIAVILATHNTDSGKLGARKASELGINKENGKIEFAQLYGMSDALSFGL--KRAGFNV 428
Query: 422 IKYVPWGPPLETKDYLLRRLQEN 444
KY+P+GP YL+RR EN
Sbjct: 429 SKYMPYGPVDTAIPYLIRRAYEN 451
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,255,309
Number of Sequences: 60738
Number of extensions: 1169812
Number of successful extensions: 3010
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2987
Number of HSP's gapped (non-prelim): 10
length of query: 467
length of database: 30,389,216
effective HSP length: 110
effective length of query: 357
effective length of database: 23,708,036
effective search space: 8463768852
effective search space used: 8463768852
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)