ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4283 good U KOG2347 Intracellular trafficking, secretion, and vesicular transport Sec5 subunit of exocyst complex
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4283 1455731 1452975 -919
(919 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR166c [U] KOG2347 Sec5 subunit of exocyst complex 780 0.0
HsM20127584 [U] KOG2347 Sec5 subunit of exocyst complex 82 4e-15
Hs21361625 [U] KOG2347 Sec5 subunit of exocyst complex 82 4e-15
At1g21170 [U] KOG2347 Sec5 subunit of exocyst complex 60 1e-08
At1g76850 [U] KOG2347 Sec5 subunit of exocyst complex 55 6e-07
>YDR166c [U] KOG2347 Sec5 subunit of exocyst complex
Length = 971
Score = 780 bits (2013), Expect = 0.0
Identities = 425/978 (43%), Positives = 603/978 (61%), Gaps = 77/978 (7%)
Query: 5 FDVSEDQLLQFYHLNSLNPSTSWKDDSTLIYQLEDGQWEQDNLETSYAVLNDLLQQEHEF 64
F + ++QLL+FY L ++NP+ SW DS+ + E+ + +ETS+ +L D + ++
Sbjct: 4 FQIGDEQLLRFYQLKTINPTHSWAQDSSKLNN-EEATSNELGVETSFDILKDF-KYGNQI 61
Query: 65 NRKKGSGANSERED---VEDPLNPSTPAMELLRRVSNDPSSNNSHEYLISSKSFKPKLYL 121
+ K S A E + DPLN E+ + + + P+ + YL++SK F K +L
Sbjct: 62 SIDKESRAYLNDESLSYIRDPLN----GQEMSKELQHLPNDSMRLNYLVNSKQFNVKAFL 117
Query: 122 KKVHPQDTFEQLTKSLDVLDKSLQEQSYELKQLVQTNFAKYVRSKSNLDHIYKRFDTLMN 181
+ +H QD+F L SLD LD +Q+QS LKQLV NF KYV+ K+ LD IYK FD N
Sbjct: 118 RDMHKQDSFNDLNNSLDRLDSDIQDQSIHLKQLVGKNFTKYVKIKNKLDQIYKEFDEKTN 177
Query: 182 SAD-AETYDEDELSVQRLNENLNETMKQTSLKLQPLLSNNDKLQKFQWAIQFVNENRYFF 240
+ ++ E++++V+ LN+ ++E ++ T+ KL+PL+ N K+ +Q +F+ N+++F
Sbjct: 178 EKNQCDSPKENQINVESLNKKVDEVIRTTTFKLKPLMDNYQKILNYQATKKFIELNKFYF 237
Query: 241 DLPRXXXXXXXXXDYTNLMFEYEKAQTAYKSLSARNTEDLHINNDNSVIEKIWNEVELIM 300
+LP+ D+ + EY K T + + + + + VI++IW ++E ++
Sbjct: 238 NLPKSLKRCLTNNDFNEFIIEYSKGLTLRRRFNQSSDA-----SQSLVIKRIWTQIENLL 292
Query: 301 DRYRKYSWAKLLMPPAIGSSDSQEVFLPLLSKLIDLK--------------------VTE 340
Y+ W L+ QE L L SKL++L+ E
Sbjct: 293 VTYKDLIWNSLINSN-FNIDQPQETILSLFSKLLNLENFINNNQRESESGNKNTTSSSNE 351
Query: 341 NPIIMWIDTRMNNFQDQLKRTSSQMIENITHSTETLLENSSGND-----LDLHFYLSMN- 394
NPI+ W+ +MN FQ++L S MI I HS +L+N++ D ++L +YL +N
Sbjct: 352 NPILRWMSIKMNGFQNELNELSGHMISKIIHSQRLILQNNTNQDKSQGCVELSYYLKINQ 411
Query: 395 ---IYSDDAT----VKNDAE------LASTPHHALMVQ---RGLTDVPAVVETWLLTLKF 438
I SD +K+ E ++ T + L Q +GLTD P ++E WLL LK+
Sbjct: 412 LFQIISDTGKDSEGLKSTVEPNKVNTISGTSYLNLNCQPSSQGLTDSPTIIEMWLLILKY 471
Query: 439 MNGLNNISTSFVEFWEHVERFLDNTYQTMLLNERKQKKIIESNDELSADDGAMLRFSEEE 498
+N L I F+EFWEH+E+FLD TYQ ++NE++++ I+ + + L EE+
Sbjct: 472 INDLWKICDQFIEFWEHIEKFLDGTYQNSIINEKRKENILIGDSNIIESYQKSLILKEEQ 531
Query: 499 AQIVKRQGSSFINAISKVLLNFFRSTQENIGI-------ENVPLSKDSGTPLSYGFIPLR 551
V+ +G FI ++S+ L++FF S+Q ++ + +KDSG+PL YGFIP
Sbjct: 532 INEVRLKGEEFITSVSQNLISFFTSSQSSLPSSLKDSTGDITRSNKDSGSPLDYGFIPPN 591
Query: 552 SNCLSCLRYLPKIADPIFRFTTELAQLAIDTESIETLRKLDAFIIDRCVSAISSTRLRDL 611
N LSCLRYLPKI +PI +F+TELAQL I T I R + II+RCV AISST+LRD+
Sbjct: 592 CNGLSCLRYLPKIVEPILKFSTELAQLNITTNGITICRNTLSTIINRCVGAISSTKLRDI 651
Query: 612 SLMYVLEDWEVYD-------SYDDDR-----YSITQFPEIARSLQEYSIQTLHEILFFYE 659
S Y LE+W+VY+ S D + Y +TQFPEI S QE SI+T ++LF YE
Sbjct: 652 SNFYQLENWQVYETVTFSSKSQDSSKNLTFEYGVTQFPEIVTSFQEVSIKTTRDLLFAYE 711
Query: 660 KLPVIKGISIIGYPSKQLLTSIEIQQXXXXXXXXXXXXKNATKDKDNPRNSHTVLTLNNL 719
KLP+I GIS++ YPSKQLLT IEIQQ KNA KDKDNPRNSHT+LTL NL
Sbjct: 712 KLPIINGISVVSYPSKQLLTGIEIQQIISMEAVLEAILKNAAKDKDNPRNSHTILTLTNL 771
Query: 720 KHIKSQTFPKVLNHFDEAFEMSLSSKNLELYNLLAKMESSIFGNYLSDLKFSLRDKLEDK 779
++ + FP +L +FD+AFE +L+SKNLEL++LL+KMESSIFGNYLSDLK +LRD LE+K
Sbjct: 772 QYFRECAFPNILQYFDDAFEWNLASKNLELFSLLSKMESSIFGNYLSDLKINLRDTLEEK 831
Query: 780 FHDINWSAYTSNSFRVSDYIIETLMVLISTHSECFRIGPQLIERILKETQVFMAKYLFEA 839
FH+INW YTSNSFRV DYIIE LM+LI HSECFRIGPQLI +IL ETQ+F+A+YLFEA
Sbjct: 832 FHEINWPMYTSNSFRVGDYIIEALMILIVVHSECFRIGPQLIHKILIETQIFIARYLFEA 891
Query: 840 FKPYIGNISSDGLLQVTVDLCFFEKVLKGLLVEDTRTIIMACLQNCFQNDVPRMQRIIKE 899
FKPY+GN+S+DG LQ+ VDL FF+KV+ LL +DT + ACLQNCFQND R+Q+ I E
Sbjct: 892 FKPYVGNLSNDGSLQIIVDLEFFQKVMGPLLEKDTEATLRACLQNCFQNDTNRLQKCINE 951
Query: 900 TEPIVNSNLDRTKIQFAS 917
PIV++NL RT IQFA+
Sbjct: 952 INPIVSANLKRTAIQFAA 969
>HsM20127584 [U] KOG2347 Sec5 subunit of exocyst complex
Length = 774
Score = 82.0 bits (201), Expect = 4e-15
Identities = 152/807 (18%), Positives = 302/807 (36%), Gaps = 119/807 (14%)
Query: 111 SSKSFKPKLYLKKVHPQDTFEQLTKSLDVLDKSLQEQSYELKQLVQTNFAKYVRSKSNLD 170
+S++F YL + H +FEQL ++ L + ++S V+ + + ++ L
Sbjct: 11 TSENFSAAWYLIENHSNTSFEQLKMAVTNLKRQANKKSEGSLAYVKGGLSTFFEAQDALS 70
Query: 171 HIYKRFDTLMNSADAETYDEDELSVQRLNENLNETMKQTSLKLQPLLSNNDKLQKFQWAI 230
I+++ + AD E ++ Q+L LN Q +L DK + A+
Sbjct: 71 AIHQKLE-----ADGTEKVEGSMT-QKLENVLNRASNTADTLFQEVLGRKDKADSTRNAL 124
Query: 231 QFVNENRYFFDLPRXXXXXXXXXDYTNLMFEYEKAQTAYKSLSARNTEDLHINNDNSVIE 290
+ ++ F+LP DY ++ +YEKA++ + + V +
Sbjct: 125 NVLQRFKFLFNLPLNIERNIQKGDYDVVINDYEKAKSLFGKTEVQ------------VFK 172
Query: 291 KIWNEVELIMDRYRKYSWAKLLMPPAIGSSDSQEVFLPLLSKLIDLKVTENPIIMWIDTR 350
K + EVE ++ R+ KLL P+ + Q+ ++ LS DL + +P I +
Sbjct: 173 KYYAEVETRIEALRELLLDKLLETPS--TLHDQKRYIRYLS---DLHASGDPAWQCIGAQ 227
Query: 351 MNNFQDQLKRTSSQMIENI-----THSTETLLENSSGNDLDLHF--YLSMNIYSDDATVK 403
+ ++++ HS L+N + + H S+ S + +
Sbjct: 228 HKWILQLMHSCKEGYVKDLKGNPGLHSPMLDLDNDTRPSVLGHLSQTASLKRGSSFQSGR 287
Query: 404 NDAELASTPHHALMVQRGLTD--VPAVVETWLLTLKFMNGLNNISTSFVEFWEHVERFLD 461
+D TPH V++ LT + + W L + ++NG
Sbjct: 288 DDTWRYKTPHRVAFVEK-LTKLVLSQLPNFWKLWISYVNG-------------------- 326
Query: 462 NTYQTMLLNERKQKKIIESNDELSADDGAMLRFSEEEAQIVKRQGSSFINAISKVLLNFF 521
L +E +K G + E ++ V+++ + F I +V+ +
Sbjct: 327 -----SLFSETAEK------------SGQI-----ERSKNVRQRQNDFKKMIQEVMHSLV 364
Query: 522 RSTQENIGIENVPLSKDSGTPLSYGFIPLRSNCLSCLRYLPKIADPIFRFTTELAQLAID 581
+ T+ + +PLS G YG ++ C ++L + L L I
Sbjct: 365 KLTRGAL----LPLSIRDGEAKQYGGWEVK--CELSGQWLAHAIQTVRLTHESLTALEIP 418
Query: 582 TESIETLRKLDAFIIDRCVSAISSTRLRDLSLMYVLEDWEVYDSYDDDRYSITQFPEIAR 641
+ ++T++ L + RCV A ++ + EDW V D +T P
Sbjct: 419 NDLLQTIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIV------DNEGLTSLPCQFE 472
Query: 642 SLQEYSIQTLHEILFFYEKLPVIKGISIIGYPSKQ---LLTSIEIQQXXXXXXXXXXXXK 698
S+Q+L +L E P S+ P Q SI I Q
Sbjct: 473 QCIVCSLQSLKGVL---ECKP--GEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKP 527
Query: 699 NATKDKDN--------------------PRNSHTVLTLNNLKHIKSQTFPKVLNHFDEAF 738
+A D + ++ L+N +++ TF + HF++
Sbjct: 528 DADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHN 587
Query: 739 EMSLSSKNLELYNLLAKMESSIFGNYLSDLKFSLRDKLEDKFHD--INWSAYTSNSFRVS 796
+ L +++ +F NY+ + LE + +W + V
Sbjct: 588 FQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPT-GVR 646
Query: 797 DYIIETLMVLISTHSECFRIGPQLIERILKETQVFMAKYLFEAFKPYIGNISSDGLLQVT 856
+Y+ E L+ +I+ H+E F I +L+ R+L + +++ L + + + S +G LQ
Sbjct: 647 NYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQ-CVSSFSKNGALQAR 705
Query: 857 VDLCFFEKVLKGLLVEDTRTIIMACLQ 883
+++C + L ++++ L+
Sbjct: 706 LEICALRDTVAVYLTPESKSSFKQALE 732
>Hs21361625 [U] KOG2347 Sec5 subunit of exocyst complex
Length = 924
Score = 82.0 bits (201), Expect = 4e-15
Identities = 152/807 (18%), Positives = 302/807 (36%), Gaps = 119/807 (14%)
Query: 111 SSKSFKPKLYLKKVHPQDTFEQLTKSLDVLDKSLQEQSYELKQLVQTNFAKYVRSKSNLD 170
+S++F YL + H +FEQL ++ L + ++S V+ + + ++ L
Sbjct: 161 TSENFSAAWYLIENHSNTSFEQLKMAVTNLKRQANKKSEGSLAYVKGGLSTFFEAQDALS 220
Query: 171 HIYKRFDTLMNSADAETYDEDELSVQRLNENLNETMKQTSLKLQPLLSNNDKLQKFQWAI 230
I+++ + AD E ++ Q+L LN Q +L DK + A+
Sbjct: 221 AIHQKLE-----ADGTEKVEGSMT-QKLENVLNRASNTADTLFQEVLGRKDKADSTRNAL 274
Query: 231 QFVNENRYFFDLPRXXXXXXXXXDYTNLMFEYEKAQTAYKSLSARNTEDLHINNDNSVIE 290
+ ++ F+LP DY ++ +YEKA++ + + V +
Sbjct: 275 NVLQRFKFLFNLPLNIERNIQKGDYDVVINDYEKAKSLFGKTEVQ------------VFK 322
Query: 291 KIWNEVELIMDRYRKYSWAKLLMPPAIGSSDSQEVFLPLLSKLIDLKVTENPIIMWIDTR 350
K + EVE ++ R+ KLL P+ + Q+ ++ LS DL + +P I +
Sbjct: 323 KYYAEVETRIEALRELLLDKLLETPS--TLHDQKRYIRYLS---DLHASGDPAWQCIGAQ 377
Query: 351 MNNFQDQLKRTSSQMIENI-----THSTETLLENSSGNDLDLHF--YLSMNIYSDDATVK 403
+ ++++ HS L+N + + H S+ S + +
Sbjct: 378 HKWILQLMHSCKEGYVKDLKGNPGLHSPMLDLDNDTRPSVLGHLSQTASLKRGSSFQSGR 437
Query: 404 NDAELASTPHHALMVQRGLTD--VPAVVETWLLTLKFMNGLNNISTSFVEFWEHVERFLD 461
+D TPH V++ LT + + W L + ++NG
Sbjct: 438 DDTWRYKTPHRVAFVEK-LTKLVLSQLPNFWKLWISYVNG-------------------- 476
Query: 462 NTYQTMLLNERKQKKIIESNDELSADDGAMLRFSEEEAQIVKRQGSSFINAISKVLLNFF 521
L +E +K G + E ++ V+++ + F I +V+ +
Sbjct: 477 -----SLFSETAEK------------SGQI-----ERSKNVRQRQNDFKKMIQEVMHSLV 514
Query: 522 RSTQENIGIENVPLSKDSGTPLSYGFIPLRSNCLSCLRYLPKIADPIFRFTTELAQLAID 581
+ T+ + +PLS G YG ++ C ++L + L L I
Sbjct: 515 KLTRGAL----LPLSIRDGEAKQYGGWEVK--CELSGQWLAHAIQTVRLTHESLTALEIP 568
Query: 582 TESIETLRKLDAFIIDRCVSAISSTRLRDLSLMYVLEDWEVYDSYDDDRYSITQFPEIAR 641
+ ++T++ L + RCV A ++ + EDW V D +T P
Sbjct: 569 NDLLQTIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIV------DNEGLTSLPCQFE 622
Query: 642 SLQEYSIQTLHEILFFYEKLPVIKGISIIGYPSKQ---LLTSIEIQQXXXXXXXXXXXXK 698
S+Q+L +L E P S+ P Q SI I Q
Sbjct: 623 QCIVCSLQSLKGVL---ECKP--GEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKP 677
Query: 699 NATKDKDN--------------------PRNSHTVLTLNNLKHIKSQTFPKVLNHFDEAF 738
+A D + ++ L+N +++ TF + HF++
Sbjct: 678 DADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHN 737
Query: 739 EMSLSSKNLELYNLLAKMESSIFGNYLSDLKFSLRDKLEDKFHD--INWSAYTSNSFRVS 796
+ L +++ +F NY+ + LE + +W + V
Sbjct: 738 FQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPT-GVR 796
Query: 797 DYIIETLMVLISTHSECFRIGPQLIERILKETQVFMAKYLFEAFKPYIGNISSDGLLQVT 856
+Y+ E L+ +I+ H+E F I +L+ R+L + +++ L + + + S +G LQ
Sbjct: 797 NYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQ-CVSSFSKNGALQAR 855
Query: 857 VDLCFFEKVLKGLLVEDTRTIIMACLQ 883
+++C + L ++++ L+
Sbjct: 856 LEICALRDTVAVYLTPESKSSFKQALE 882
>At1g21170 [U] KOG2347 Sec5 subunit of exocyst complex
Length = 1090
Score = 60.5 bits (145), Expect = 1e-08
Identities = 59/289 (20%), Positives = 120/289 (41%), Gaps = 33/289 (11%)
Query: 81 DPLNPSTPAMELLRRVSNDPSS--------NNSHEYLI-SSKSFKPKLYLKKVHPQDTFE 131
DPL + LR ++ S N E L+ +S F PKL++ ++H + +
Sbjct: 188 DPLKLGIIDNKTLRLITESSESLSKAEKVDNALREKLVYTSDHFDPKLFISRIHQETSAA 247
Query: 132 QLTKSLDVLDKSLQEQSYELKQLVQTNFAKYVRSKSNLDHIYKRFDTLMNSADAETYDED 191
L L L+ ++ + KQLV+ NF +V K+ +D I + + + D D
Sbjct: 248 DLESGALALKSDLKGRNLQRKQLVKDNFDCFVSCKTTIDDIESKLKRIED-------DPD 300
Query: 192 ELSVQRLNENLNETMKQTSLKLQPLLSNNDKLQKFQWAIQFVNENRYFFDLPRXXXXXXX 251
L + + + +PL + +K + + R F+LP
Sbjct: 301 GSGTTHLFNCMKSVTSRANRAFEPLFERQAQAEKIRSVQGMLQRFRTLFNLPSIIRSSIS 360
Query: 252 XXDYTNLMFEYEKAQTAYKSLSARNTEDLHINNDNSVIEKIWNEVELIMDRYRKYSWAKL 311
+Y + EY+KA KS++ + H+N +++++ EVE +M ++ + +
Sbjct: 361 KGEYDLAVREYKKA----KSIALPS----HVN----LLKRVLEEVEKVMQEFKGTLYRSM 408
Query: 312 LMPPAIGSSDSQEVFLPLLSKLIDLKVTENPIIMWIDTRMNNFQDQLKR 360
P +S V L L++L+ +P+ +++ + + L++
Sbjct: 409 EDPKIDFTSLENTVRL-----LLELEPESDPVWHYLNVQNHRIHGMLEK 452
>At1g76850 [U] KOG2347 Sec5 subunit of exocyst complex
Length = 1085
Score = 54.7 bits (130), Expect = 6e-07
Identities = 43/184 (23%), Positives = 74/184 (39%), Gaps = 13/184 (7%)
Query: 90 MELLRRVSNDPSS-----NNSHEYLIS-SKSFKPKLYLKKVHPQDTFEQLTKSLDVLDKS 143
+ L+ S PS N E L+ S F PKL+L ++H T L L
Sbjct: 201 LRLITESSGSPSKAEKVDNTLREKLVYFSDHFDPKLFLSRIHQDTTAADLEAGALGLKSD 260
Query: 144 LQEQSYELKQLVQTNFAKYVRSKSNLDHIYKRFDTLMNSADAETYDEDELSVQRLNENLN 203
L+ ++ + KQLV+ NF +V K+ +D I + + D + L +
Sbjct: 261 LKGRNLQRKQLVKDNFDCFVSCKTTIDDIESKLKRIEE-------DPEGSGTTHLFNCMK 313
Query: 204 ETMKQTSLKLQPLLSNNDKLQKFQWAIQFVNENRYFFDLPRXXXXXXXXXDYTNLMFEYE 263
+ +L +PL + +K + + R F+LP +Y + EY+
Sbjct: 314 SVTSRANLAFEPLFERQAQAEKIRSVQGMLQRFRTLFNLPSIIRSSISKGEYDLAVREYK 373
Query: 264 KAQT 267
KA++
Sbjct: 374 KAKS 377
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,302,981
Number of Sequences: 60738
Number of extensions: 2221904
Number of successful extensions: 7491
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 7475
Number of HSP's gapped (non-prelim): 10
length of query: 919
length of database: 30,389,216
effective HSP length: 116
effective length of query: 803
effective length of database: 23,343,608
effective search space: 18744917224
effective search space used: 18744917224
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)