ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4292 good U KOG2034 Intracellular trafficking, secretion, and vesicular transport Vacuolar sorting protein PEP3/VPS18
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4292 1456620 1459391 924
(924 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR148w [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 543 e-154
SPCC790.02 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 253 1e-66
At1g12470 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 252 2e-66
HsM17978485 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 236 9e-62
7290189 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 170 1e-41
CE25141 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 135 2e-31
Hs17978487 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18 59 3e-08
>YLR148w [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 918
Score = 543 bits (1399), Expect = e-154
Identities = 342/955 (35%), Positives = 543/955 (56%), Gaps = 86/955 (9%)
Query: 14 ISCQVEHVSLKFIQEVHQNLCCLQVVSNTMFIALKTGHIFIINLNDPATVHSFILPMLAN 73
I ++E V L+F+ + L L+V SN I I+ INL DPA V+ F P L+
Sbjct: 2 IKTRIEEVQLQFLTG-NTELTHLKV-SNDQLIVTTQRTIYRINLQDPAIVNHFDCP-LSK 58
Query: 74 SQEKLLCIWVNPQASLILFKTNFAKYFLGHVKRIVDGGKNSKLTTLKRLNKKSCDIRTVD 133
E ++ + V+P S+IL +TNF +Y L K+ + T L ++ K+ D+ ++
Sbjct: 59 ELETIMNVHVSPMGSVILIRTNFGRYMLL---------KDGEFTQLNKI--KNLDLSSLH 107
Query: 134 WSFDESTLLVGTKEG-KAYHIYLGPCVKNSNDDAQVTRIFSATES----IDGIMFNKSLG 188
W +E+T L+G K+ K Y + L + D T+++ + IDGI + +
Sbjct: 108 W-INETTFLMGIKKTPKLYRVEL------TGKDI-TTKLWYENKKLSGGIDGIAYWEG-S 158
Query: 189 ALLIIGSQIMYWDSSISQKLGPIEVFSSTKPTATEQYQQTDKEFGSRFTFMGSKFAWVTQ 248
LL I I+YW + K F P +EQ+++ +F FAWVT
Sbjct: 159 LLLTIKDNILYWRDVTNMK------FPLVLPDESEQFERLKHHAIKKFDSYNGLFAWVTS 212
Query: 249 SGIVVGDLNDE------------KVLANARVLLYIELPA----STHRVKDVRLTKYHLVL 292
+GIV GDL ++ K L++++VLL ELP H +KD+ LT +H++L
Sbjct: 213 NGIVFGDLKEKQMEKDPASNNFGKFLSSSKVLLNFELPDYQNDKDHLIKDIVLTAFHILL 272
Query: 293 LRGSEIIVVNQLTMKIVFQESI-------FNSNDIEKLHSLCIDYSQVPPTTWCHSASNV 345
LR + + +V+QL +VF E+I N++ EK L D V T WC S NV
Sbjct: 273 LRKNTVTMVSQLNNDVVFHETIPRHQLTGSNTDSNEKFLGLVRD--SVKETFWCFSNINV 330
Query: 346 YEIVMSGEDRSVWKLLCDTHRFEEALALDGLSSVQKDYVHSFYGDY-YYEKQMWEEAAEQ 404
+EI++ E SVW LL ++F++AL+L GL+ + + V Y ++ + + AA+
Sbjct: 331 FEIIIENEPNSVWNLLVRDNKFDKALSLKGLTVREIESVKLSKAMYLFHTAKDFHSAAQT 390
Query: 405 YSKTKMLNNCGPIALKFMKDSSQIASLQLLLRNYLSSTNDDYQVKQVILTSWIIQNYMNQ 464
K L++ G IAL F++ L ++L L N ++ QV+L+SWII N+M Q
Sbjct: 391 LGSMKDLSHFGEIALNFLQ-IKDYNDLNVILIKQLD--NVPWKSTQVVLSSWIIWNFMKQ 447
Query: 465 LNDIDEKINR----DASDEKLASQKTDIVKK---FESFVTQNFNKMDKETIYQIISRQNR 517
LNDI+ KIN ++ L + ++ +K F+ + K+D ET+YQI+S+QNR
Sbjct: 448 LNDIELKINTTKPASTDEDNLLNWNLNLKEKSNELTKFLESHLEKLDNETVYQIMSKQNR 507
Query: 518 KKELLYFATLAEDYEYVLSYWIKLENWYESLKLLSSLQEPDLIYKYSNILLISSPDATIN 577
+ ELL FA+L D +++LS+WI NWYESLK+L ++ DL+YKYS ILL++SP+AT++
Sbjct: 508 QNELLIFASLINDMKFLLSFWIDQGNWYESLKILLTINNHDLVYKYSLILLLNSPEATVS 567
Query: 578 TWMQITSLDPVPLIPSILSYFTNYQ-KKKRAEGIHSNVQNHGVNYLKWCIRDQNNTV-PI 635
TWM+I LDP LIP+IL +FTN+Q K I +N+ + YLKWC+R+ PI
Sbjct: 568 TWMKIKDLDPNKLIPTILKFFTNWQNNSKLITNISEYPENYSLTYLKWCVREVPKMCNPI 627
Query: 636 IHNTYLYMMIVDKTTDK--DQEVIQFLTKHSKGHFDTDFILRLSLRYERYGVSICIYSEI 693
++N+ LYMMI D D + ++I+F+ K ++ +D +F LRLSL++++ SI + + +
Sbjct: 628 VYNSILYMMITDPRNDMILENDIIKFM-KSNENKYDLNFQLRLSLKFKKTKTSIFLLTRL 686
Query: 694 SLYEEAVSLALKHGRLQEAKKVAT---TVEDPDIKKTLWLDIAAAMI--KQDRDIKNTLT 748
+L+E+A+ LALK+ + + K + +ED ++K LWL IA ++ +D DIK +
Sbjct: 687 NLFEDAIDLALKNNLIDDCKVIVNDEILIEDYKLRKRLWLKIAKHLLLSMKDIDIKQLIR 746
Query: 749 LLIQESEGVLSVKDLLPLFDEFVTIAKLKEELVRSLEKHSMQITKLSQEIRDSLKIKSEI 808
++ +S +L++KDLLP F+E+ TIA LKEEL++ LE H+M++ ++S++I +S +K EI
Sbjct: 747 TILNDSNEILTIKDLLPFFNEYTTIANLKEELIKFLENHNMKMNEISEDIINSKNLKVEI 806
Query: 809 KNDIILFRNRYEKLKPGASCSYCSQPLQTRKFFVYPCGHSINTDCIIKIIMASNQYALKA 868
+I F Y L+PG SC C + LQ +KF V+PCGH + +CII++I+ SN Y L+
Sbjct: 807 NTEISKFNEIYRILEPGKSCDECGKFLQIKKFIVFPCGHCFHWNCIIRVILNSNDYNLRQ 866
Query: 869 KIQNMQK-KLLRDRNSVKSXXXXXXXSAQCPLCSEISINSIDEPWEVDPNTALKW 922
K +N K K + N +++ +C LCS+I+IN ID+P +D KW
Sbjct: 867 KTENFLKAKSKHNLNDLENIIV-----EKCGLCSDININKIDQPISIDETELAKW 916
>SPCC790.02 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 900
Score = 253 bits (645), Expect = 1e-66
Identities = 222/929 (23%), Positives = 419/929 (44%), Gaps = 101/929 (10%)
Query: 18 VEHVSLKFIQEVHQNLCCLQVVSNTMFIALKTGHIFIINLNDPATVHSFILPMLANSQEK 77
+E V L+F + CL V +N + +AL + + I++L P + LP +
Sbjct: 41 LEKVQLQFPVSIR----CLAVENNILVMALTSDKLMIVDLERPEDIIDIELPKKVLALGL 96
Query: 78 LLCIWVNPQASLILFKTNFAKYFLGHVKRIVDGGKNSKLTTLKRLNKKSCDIRTVDWSFD 137
I+++P I T L + LT LK + V W+ +
Sbjct: 97 TYKIFLDPSGHYIFVTTTAGDNCL-----FTPSHQGRVLTKLK-----GHTVEAVQWNLN 146
Query: 138 EST---LLVGTKEGKAYHIYLGPCVKNSNDDAQVTRI---------FSATESIDGIMFNK 185
LL+ +K G + L + D A + RI F ES GI+ N
Sbjct: 147 GGNILELLIASKSGVLLELVL------TLDSANLKRIEKSINTLYSFPFMESPMGILKNI 200
Query: 186 SLGALLII--------------GSQIMYWDSSISQKLGPIEVFSSTKPTATEQYQQTDKE 231
++ I+ G +Y+ + + I FS + Y K
Sbjct: 201 QDDSMTIVTNKRILRFEPKTSRGKDQLYFSPAFQGSMKEILSFSEEETAQCFSYSPFPKN 260
Query: 232 FGSRFTFMGSKFAWVTQSGIVVGDLNDEKVLANARVLLYIELPASTHRVKDVR--LTKYH 289
+T A T I+ D+ + V + + + E P + ++ LT +H
Sbjct: 261 LAEPYTL-----ALKTSKRIIYLDIMNP-VNPDIQDYEFNESPKLSVPTVEMNMILTSFH 314
Query: 290 LVLLRGSEIIVVNQLTMKIVFQESIFNSNDIEKLHSLCIDYSQVPPTTWCHSASNVYEIV 349
L L + +VN++ K +Q+ + N + E++ LC D+ + T W ++ +++E+V
Sbjct: 315 LAFLDLDTLYIVNRVNGKESYQQRV-NLSPHEEILGLCCDHEK--NTYWLYTTDSLHELV 371
Query: 350 MSGEDRSVWKLLCDTHRFEEALALDGLSSVQKDYVHSFYGDYYYEKQMWEEAAEQYSKTK 409
++ E R + + FE+AL + V+ + Y ++ E + +E AA Y++T
Sbjct: 372 VNNETREASLVFLEKGDFEKALECANTAKVRNTVLVG-YAEFLMEHEEYERAATLYAET- 429
Query: 410 MLNNCGPIALKFMKDSSQIASLQLLLRNYLSSTNDDYQVKQVILTSWIIQNYMNQLNDID 469
L + +ALKF+ + +Q L+L L L S ++++ +L +W+++ + +LN +D
Sbjct: 430 -LKSVEEVALKFI-ELNQKDVLRLYLWKKLRSYKSTMKIQKSLLVNWLLELMLAKLNSLD 487
Query: 470 EKINRDASDEKLASQKTDIVKKFESFVTQNFNKMDKETIYQIISRQNRKKELLYFATLAE 529
EK + E + Q+ + ++F + + Q +++++E Y + + ++++LL AT+ +
Sbjct: 488 EKERLELFPENVMQQRQQVQREFSTLLNQYKDEINREAAYNLANNYGKEEQLLQIATVMK 547
Query: 530 DYEYVLSYWIKLENWYESLKLLSSLQEPDLIYKYSNILLISSPDATINTWMQITSLDPVP 589
D Y++ YW++ EN+ ++L+ L+ + + +++ LL P+ T++ W + T LD
Sbjct: 548 DQSYIMHYWVQRENYEKALETLNEGVSQETLIQHATALLTHRPNETVSIWERQTDLDVHA 607
Query: 590 LIPSILSYFTNYQKKKRAEGIHSNVQNHGVNYLKWCIRDQNNTVPIIHNTYLYMMIVDKT 649
LIPS+LSY N + E +N + YL++ P IHNT L+ + +
Sbjct: 608 LIPSLLSY--NQRSHVPVE------ENAAIRYLRYVTGVLGCVDPSIHNT-LFCIYACHS 658
Query: 650 TDKDQEVIQFLTKHSKGH-FDTDFILRLSLRYERYGVSICIYSEISLYEEAVSLALKHGR 708
+ + ++ ++ + +D D +RL L++ ++ I + LY + V LAL+
Sbjct: 659 SSNESYLMNYIEQQGNHPLYDMDLGIRLCLQFNCRRSAVKILVLMKLYSQGVELALEADD 718
Query: 709 LQEAKKVAT-TVEDPDIKKTLWLDIAAAMIKQDRDIKNTLTLLIQESEGVLSVKDLLPLF 767
+ A +A ED +KKTLW IA M + IK TL L E+ VL + +L+ L
Sbjct: 719 CELAATIANIPEEDVVLKKTLWQTIAKYMFSKKSGIKETLRFL--ENSEVLQLPELIRLL 776
Query: 768 DEFVTIAKLKEELVRSLEKHSMQITKLSQEIRDSLKIKSEIKNDIILFRNRYEKLKPGAS 827
E + + L + + L+ +I +L EI + ++ EI+ + RNRY L+P S
Sbjct: 777 PEDIKLDDLSDNVCDELDHCMKRIEQLDFEIGQASEVAHEIQTNAENMRNRYIVLEPNES 836
Query: 828 CSYCSQPLQTRKFFVYPCGHSINTDCIIKIIMASNQYALKAKIQNMQKKLLRDRNSVKSX 887
C +C+QPL + F ++PC H+ + C+++ KL ++N +K
Sbjct: 837 CWHCNQPLFSEPFVLFPCQHAFHRSCMLE----------------KTYKLASEKNILK-- 878
Query: 888 XXXXXXSAQCPLCS-EISINSIDEPWEVD 915
+C LC ++ I+EP+ D
Sbjct: 879 --------ECQLCGPSYAVRLINEPFSTD 899
>At1g12470 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 994
Score = 252 bits (643), Expect = 2e-66
Identities = 194/736 (26%), Positives = 362/736 (48%), Gaps = 90/736 (12%)
Query: 246 VTQSGIVVGDLN----------DEKVLANARVLLYIELPASTHRVK--DVRLTKYHLVLL 293
+T +GI G LN DE + N +L Y +L T VK + L++YH +LL
Sbjct: 250 LTWTGIYHGGLNFGAQHSYPNGDENFVENKALLDYSKLSDGTEAVKPGSMALSEYHFLLL 309
Query: 294 RGSEIIVVNQLTMKIV--FQESIFNSNDIEKLHSLCIDYSQVPPTTWCHSASNVYEIVMS 351
G+++ VVN+++ +I+ Q I + + + LC D S + + ++++++ +
Sbjct: 310 IGNKVKVVNRISEQIIEELQFDITSDSVSRGIIGLCSDASA--NVFYAYDQNSIFQVSVI 367
Query: 352 GEDRSVWKLLCDTHRFEEALALDGLSSVQKDYVHSFYGDYYYEKQMWEEAAEQYSKTKML 411
E R +WK+ D + ALA + +Q+D V+ + + + + AA Y+K +
Sbjct: 368 DEGRDMWKVYLDLKVYAAALA-NCRDPLQRDQVYLVQAESAFTDKEYLRAASFYAKINYV 426
Query: 412 NNCGPIALKFMKDSSQIASLQLLLRNYLSSTNDDYQVKQVILTSWIIQNYMNQLNDIDEK 471
+ + LKF+ + A L+ L + L + + D + + ++++W + Y++++N +
Sbjct: 427 ISFEEVTLKFISINEPEA-LRTFLLHKLDNLSKDDKCQITMISTWATELYLDKINRL--L 483
Query: 472 INRDASDEKLASQKTDIVKKFESFVTQNFNKMDKETIYQIISRQNRKKELLYFATLAEDY 531
+ D + E S+ ++++F +F++ +++D+ T +I+ R +EL+YFA L E Y
Sbjct: 484 LEDDTAIENRDSEYHSVIQEFRAFMSDCKDELDEATTVKILESYGRVEELVYFANLKEQY 543
Query: 532 EYVLSYWIKLENWYESLKLLS-SLQEPDLIYKYSNILLISSPDATINTWMQITSLDPVPL 590
E V+ ++I+ ++L++L S +L Y+++ L++ T+ +WM +L+P L
Sbjct: 544 EIVVLHYIQQGEAKKALEVLQKSSVSVELQYQFAPELIMLDAYETVESWMANKNLNPRRL 603
Query: 591 IPSILSYFTNYQKKKRAEGIHSNVQNHGV-NYLKWCIRDQNNTVPIIHNTYLYMMIVDKT 649
I +++ Y + G H+ + H V YL++C+ +N P IH+ L + + K
Sbjct: 604 ITAMMRY---------SSGPHAKNETHEVIKYLEFCVHRLHNEDPGIHS--LLLSLYAKQ 652
Query: 650 TDKDQEVIQFLT-KHSKGH-------FDTDFILRLSLRYERYGVSICIYSEISLYEEAVS 701
D D +++FL K KG +D + LRL L+ R + IYS +S++EEAV+
Sbjct: 653 ED-DGALLRFLQCKFGKGRENGPEFFYDPKYALRLCLKERRTRACVHIYSMMSMHEEAVA 711
Query: 702 LALKHG---RLQEAKKVATTVEDPDIKKTLWLDIAAAMIKQDRDIKNT----LTLLIQES 754
LAL+ + EA KV +D D++K LWL +A ++KQ++ K ++E+
Sbjct: 712 LALQIDPELAMAEADKVE---DDEDLRKKLWLMVAKHVVKQEKGAKRENIRKAIAFLKET 768
Query: 755 EGVLSVKDLLPLFDEFVTIAKLKEELVRSLEKHSMQITKLSQEIRDSLKIKSEIKNDIIL 814
+G+L ++D+LP F +F I KE + SLE ++ QI +L +E+ D+ + I+NDI
Sbjct: 769 DGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLKEEMNDATRGADNIRNDISA 828
Query: 815 FRNRYEKLKPGASCSYCSQPL-----------------QTRKFFVYPCGHSINTDCIIKI 857
RY + C C + + F+V+PCGHS + C+I
Sbjct: 829 LTQRYAVIDRDEECGVCKRKILMMSGDFRMAQGYSSAGPLAPFYVFPCGHSFHAQCLITH 888
Query: 858 IMASNQYALKAKIQNMQKKLL-------RDRNSVKS--------------XXXXXXXSAQ 896
+ + I ++QK+L RD N +S +++
Sbjct: 889 VTSCAHEEQAEHILDLQKQLTLLGSETRRDINGNRSDEPITSTTTADKLRSELDDAIASE 948
Query: 897 CPLCSEISINSIDEPW 912
CP C E+ IN I P+
Sbjct: 949 CPFCGELMINEITLPF 964
>HsM17978485 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 973
Score = 236 bits (603), Expect = 9e-62
Identities = 177/707 (25%), Positives = 338/707 (47%), Gaps = 61/707 (8%)
Query: 243 FAWVTQSGIVVGDLN---DEKVLANARVLLYIEL--PASTHRVKDVRLTKYHLVLLRGSE 297
FAW+ G++ G L+ + +L+ RV Y E P ++ + V LT++H +LL
Sbjct: 284 FAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIV-LTQFHFLLLLADR 342
Query: 298 IIVVNQLTMKIVFQESIFNSNDIEKLHSLCIDYSQVPPTTWCHSASNVYEIVMSGEDRSV 357
+ V LT ++V ++ L + D S W ++ V+ + E R V
Sbjct: 343 VEAVCTLTGQVVLRDHFLEK--FGPLKHMVKDSST--GQLWAYTERAVFRYHVQREARDV 398
Query: 358 WKLLCDTHRFEEALALDGLSSVQKDYVHSFYGDYYYEKQMWEEAAEQYSKTKMLNNCGPI 417
W+ D +RF+ A D V + D+ + ++ + E+A Y+ T+ I
Sbjct: 399 WRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFE--EI 456
Query: 418 ALKFMKDSSQIASLQLLLRNYLSSTNDDYQVKQVILTSWIIQNYMNQLNDIDEKINRDAS 477
ALKF++ + A + L R L+S + + +LT+W+ + Y+++L +
Sbjct: 457 ALKFLEARQEEALAEFLQRK-LASLKPAERTQATLLTTWLTELYLSRLGALQ-------G 508
Query: 478 DEKLASQKTDIVKKFESFVTQNFNK----MDKETIYQIISRQNRKKELLYFATLAEDYEY 533
D + + + + F +F++ +K + +I+++++ + ++YFA + +DYE
Sbjct: 509 DPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYER 568
Query: 534 VLSYWIKLENWYESLKLLSSLQEPDLIYKYSNILLISSPDATINTWMQITS-LDPVPLIP 592
V++Y + E + E+L +L+ ++P L YK+S IL+ P ++ W+++ S LD LIP
Sbjct: 569 VVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIP 628
Query: 593 SILSYFTNYQKKKRAEGIHSNVQNHGVNYLKWCIRDQNNTVPIIHNTYLYMMIVDKTTDK 652
++++Y ++G + + Y+++C+ T IHN L + +
Sbjct: 629 ALVNY---------SQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSL 679
Query: 653 DQEVIQFLTKHSKGHFDTDFILRLSLRYERYGVSICIYSEISLYEEAVSLALKHGRLQEA 712
+ Q + H+D + LRL + + + +Y + LYEEAV LAL+ + A
Sbjct: 680 LAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVD-VDLA 738
Query: 713 KKVATTVE-DPDIKKTLWLDIAAAMIKQDRDIKNTLTLLIQESEGVLSVKDLLPLFDEFV 771
K+ A E D +++K LWL IA +++++ D++ + L S +L ++D+LP F +FV
Sbjct: 739 KQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLA--SCPLLKIEDVLPFFPDFV 796
Query: 772 TIAKLKEELVRSLEKHSMQITKLSQEIRDSLKIKSEIKNDIILFRNRYEKLKPGASCSYC 831
TI KE + SL+ ++ I +L +E+ ++ I+ D+ R RY ++P C+ C
Sbjct: 797 TIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATC 856
Query: 832 SQPLQTRKFFVYPCGHSINTDCIIKIIMASNQYALKAKIQNMQKKLLRDRNSVKSXXXXX 891
PL R F+++ CGH + DC+++ + +A+++ +Q+KL K
Sbjct: 857 DFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAK 916
Query: 892 XX----------------------SAQCPLCSEISINSIDEPWEVDP 916
+A+C C E+ I SID P+ +DP
Sbjct: 917 EAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPF-IDP 962
>7290189 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 1002
Score = 170 bits (430), Expect = 1e-41
Identities = 160/723 (22%), Positives = 327/723 (45%), Gaps = 81/723 (11%)
Query: 242 KFAWVTQSGIVVGDLNDEKVLANARV---LLYIELPASTHRVKDVR---------LTKYH 289
++AW+ GI VG+L+ E A + L+ ++ + H R LT+YH
Sbjct: 303 QWAWLCGEGIRVGELSIEANSAATLIGNTLINLDFEKTMHLSYGERRLNTPKAFVLTEYH 362
Query: 290 LVLLRGSEIIVVNQLTMKIVFQESIFNSNDIEKLHSLCIDYSQVPPTTWCHSASNVYEIV 349
VLL + + L + V+QE+ F+ + K L I+ ++ + + ++ V+ +
Sbjct: 363 AVLLYADHVRAICLLNQEQVYQEA-FDEARVGK--PLSIERDELTGSIYVYTVKTVFNLR 419
Query: 350 MSGEDRSVWKLLCDTHRFEEALALDGLSSVQKDYVHSFYGDYYYEKQMWEEAAEQYSKTK 409
++ E+R+VW++ D ++E A A V D + ++ AA+ Y++T
Sbjct: 420 VTREERNVWRIYLDKGQYELATAHAAEDPEHLQLVLCQRADAAFADGSYQVAADYYAETD 479
Query: 410 MLNNCGPIALKFMKDSSQIASLQLLLRNYLSSTNDDYQVKQV---------ILTSWIIQN 460
+ + LKFM + + + + T + ++ L W+I
Sbjct: 480 --KSFEEVCLKFMVLPDKRPIINYVKKRLSRVTTKPMETDELDEDKMNIIKALVIWLIDL 537
Query: 461 YMNQLNDIDEKINRDASDEKLASQKTDIVKKFESFVTQNFNKMDKETIYQIISRQNRKKE 520
Y+ Q+N D+ +E +S +T+ + + + ++ET+ Q+I+ +
Sbjct: 538 YLIQINMPDK------DEEWRSSWQTEYDEFMMEAHVLSCTRQNRETVRQLIAEHADPRN 591
Query: 521 LLYFATLAEDYEYVLSYWIKLENWYESLKLLSSLQEPDLIYKYSNILLISSPDATINTWM 580
+ FA DY+ V++ +K E + E+L+ L + + P+L YKY+ L+ P T++ M
Sbjct: 592 MAQFAIAIGDYDEVVAQQLKAECYAEALQTLINQRNPELFYKYAPELITRLPKPTVDALM 651
Query: 581 -QITSLDPVPLIPSILSYFTNYQKKKRAEGIHSNVQNHGVNYLKWCIRDQNNTVPIIHNT 639
Q + L+ L+P+++ N +++++ + YL++ I N T IHN
Sbjct: 652 AQGSRLEVEKLVPTLI-IMENREQREQTQ-----------RYLEFAIYKLNTTNDAIHNF 699
Query: 640 YLYMMIVDKTTDKDQEVIQFLTKHSKG----HFDTDFILRLSLRYERYGVSICIYSEISL 695
L++ + + ++++L + H+D + ++ + + + +
Sbjct: 700 LLHLY----AEHEPKLLMKYLEIQGRDESLVHYDIYYAHKVCTDLDVKEARVFLECMLRK 755
Query: 696 YEEAVSLALKHGRLQEAKKVATTVEDPDIKKTLWLDIAAAMIKQDRDIKNTLTLLIQESE 755
+ AV LAL ++ AK+ A+ D I++ LWL IA IK D+K L LL +
Sbjct: 756 WISAVDLALTFD-MKLAKETASRPSDSKIRRKLWLRIAYHDIKGTNDVKKALNLL--KEC 812
Query: 756 GVLSVKDLLPLFDEFVTIAKLKEELVRSLEKHSMQITKLSQEIRDSLKIKSEIKNDIILF 815
+L ++DLLP F +F I KE + +L ++ +I +L +E+ ++ + + ++
Sbjct: 813 DLLRIEDLLPFFADFEKIDNFKEAICDALRDYNQRIQELQREMAETTEQTDRVTAELQQL 872
Query: 816 RNRYEKLKPGASCSYCSQPLQTRKFFVYPCGHSINTDCIIKII-----------MASNQY 864
R ++ +C C L + FF++ CGH ++DC+ K + + + +
Sbjct: 873 RQHSLTVESQDTCEICEMMLLVKPFFIFICGHKFHSDCLEKHVVPLLTKEQCRRLGTLKQ 932
Query: 865 ALKAKIQNMQK------------KLLRDRNSVKSXXXXXXXSAQCPLCSEISINSIDEPW 912
L+A++Q + +L R R ++K+ +A C C + I++ID+P+
Sbjct: 933 QLEAEVQTQAQPQSGALSKQQAMELQRKRAALKT-EIEDILAADCLFCG-LLISTIDQPF 990
Query: 913 EVD 915
D
Sbjct: 991 VDD 993
>CE25141 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 1010
Score = 135 bits (341), Expect = 2e-31
Identities = 177/853 (20%), Positives = 351/853 (40%), Gaps = 163/853 (19%)
Query: 203 SISQKLGPIEVFSS--TKPTATEQYQQTDKEFGSRFTFMG-----------SKFAWVTQS 249
+++ + P F S T P T Q+ + +F +F G ++AW++
Sbjct: 177 NLATEQAPSTTFHSFFTSPN-TLQHTISSSKFSEKFKNHGFLTMHPTIAEPKRYAWLSPD 235
Query: 250 GIVVGDLNDEKVLANARVLLYIELPASTHRVKDVRL--------TKYHLVLLRGSEIIVV 301
GI +G++N + A + +E HR+ + RL T YH++L S ++ +
Sbjct: 236 GISIGNVN---IYAERIQDVLVEEFNIEHRLIEGRLEPPTGIALTDYHVLLAYSSRVLAL 292
Query: 302 NQLTMKIVFQESIFNSNDIEKLHSLCIDYSQVPPTTWCHSASNVYEIVMSGEDRSVWKLL 361
+ L V E +N E +L V W ++ + + + E R +WK
Sbjct: 293 SLLPPHDVIFEDPWNP---ELGGALGFVSDNVAEFVWLYTQTFAMKYGTNDEARYIWKTY 349
Query: 362 CDTHRFEEALALDGLS-SVQKDYVHSFY---GDYY-------------------YEKQMW 398
D +++AL + +++ D + D+Y ++++ +
Sbjct: 350 LDRGEYQKALQIARTRVAIEPDALEMVLRKQADFYIQEKNLTAAVYMRKTFFLGFDQKKF 409
Query: 399 EEAAEQYSKTKMLNNCGP------------------------------IALKFMKDSSQI 428
+ +Q KTK NC P + LKF+ +SS+
Sbjct: 410 DFFQKQKKKTK---NCLPPREVHKTKFSLSSFTAAAEILAQSSEPFESVVLKFLTNSSER 466
Query: 429 A-SLQLLLRNYLSS-TNDDYQVKQVILTSWIIQNYMNQLNDID--EKINRDASDEKLASQ 484
L+ LL L T + ++++ L W++ + +L ++ + N D + +
Sbjct: 467 KMGLKTLLDKKLERLTRHEDKIRRDALVMWLLNVQLEELAEMRRLKNSNPDPAFVEKLRD 526
Query: 485 KTDIVKKFESFVTQNFNKM---DKETIYQIISRQNRKKELLYFATLAEDYEYVLSYWIKL 541
TD V+++ F+ +N + +++ +Y++ + L+FA +D V+ +
Sbjct: 527 TTDHVQRY--FMRKNVIESIQTNRDAVYRMCVAHADFEMQLFFANAVKDLRTVIDILMLR 584
Query: 542 ENWYESLKLLSSLQEPDLIYKYSNILLISSPDATINTWMQITS-LDPVPLIPSILSYFTN 600
E ++E L++L + + +L Y+ +L+ P I +Q + P L P + N
Sbjct: 585 EQYFEVLEVLKNQRISELTYEMCPLLIEHIPKQVIVYLIQNQDQISPQKLTPCLSLCVKN 644
Query: 601 YQKKKRAEGIHSNVQNHGVNYLKWCIRDQNNTVPIIHNTYLYMMIVDKTTDKDQEVIQFL 660
+ A + Q G I + +HN Y+++M + ++++ +L
Sbjct: 645 MEMAIPAIK-YLEAQFKGTQ----TISQNPQNLANLHNIYIHLM----AKFRREKLLGYL 695
Query: 661 TKHSKGHFDTDFILRLSLRY-ERYGVSICI---YSEISLYEEAVSLALKHGRLQEAKKVA 716
H D + L ++R E++ + C+ + ++ +AV AL + AKK A
Sbjct: 696 ESHGTIRSDLPYELDFAMRTCEQFKIEPCVVYLFCVAGMFGDAVEKALGFD-VDLAKKCA 754
Query: 717 TTVE-------------------------DPDIKKTLWLDIAAAMIKQDRDIKNTLTLLI 751
+E D KK +WL I + Q+ ++ + L I
Sbjct: 755 LMMEEAEANFAWLEGMEDPAATSYIRQKLDEKAKKAIWLKIGQYYVTQENNVDKCIEL-I 813
Query: 752 QESEGVLSVKDLLPLFDEFVTIAKLKEELVRSLEKHSMQITKLSQEIRDSLKIKSEIKND 811
ES +L+++DLLP+ +F + LK +V L+++ ++ KL + ++++ +I SEI++
Sbjct: 814 NESNHLLTIQDLLPIIPKFTRVGALKPIIVDFLKRNKQRLEKLERSMKEATEIASEIRDK 873
Query: 812 IILFRNRYEKLKPGASCSYCSQPLQTRKFFVYPCGHSINTDCIIKIIMASNQYALKAKIQ 871
+NR +KP CS+C++P+ R F V+ C H + +C ++I M S + + +++
Sbjct: 874 QEKLKNRTTVVKPSDVCSHCARPISGRAFNVHSCRHFFHREC-LEIAMIS--FLSQEEVE 930
Query: 872 NMQKKLLRDRNSVKS-------------------------XXXXXXXSAQCPLCSEISIN 906
M K L+ D V S A+CPLC I+I+
Sbjct: 931 KM-KTLIIDEERVLSQMKAEQLAGNQKGFIEKQEKYLKIAAFISNIVGAECPLCGNIAIS 989
Query: 907 SIDEPWEVDPNTA 919
ID+ + D A
Sbjct: 990 QIDKQFLSDEEFA 1002
>Hs17978487 [U] KOG2034 Vacuolar sorting protein PEP3/VPS18
Length = 580
Score = 59.3 bits (142), Expect = 3e-08
Identities = 57/228 (25%), Positives = 108/228 (47%), Gaps = 13/228 (5%)
Query: 243 FAWVTQSGIVVGDLN---DEKVLANARVLLYIEL--PASTHRVKDVRLTKYHLVLLRGSE 297
FAW+ G++ G L+ + +L+ RV Y E P ++ + V LT++H +LL
Sbjct: 284 FAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIV-LTQFHFLLLLADR 342
Query: 298 IIVVNQLTMKIVFQESIFNSNDIEKLHSLCIDYSQVPPTTWCHSASNVYEIVMSGEDRSV 357
+ V LT ++V ++ L + D S W ++ V+ + E R V
Sbjct: 343 VEAVCTLTGQVVLRDHFLEK--FGPLKHMVKDSST--GQLWAYTERAVFRYHVQREARDV 398
Query: 358 WKLLCDTHRFEEALALDGLSSVQKDYVHSFYGDYYYEKQMWEEAAEQYSKTKMLNNCGPI 417
W+ D +RF+ A D V + D+ + ++ + E+A Y+ T+ + I
Sbjct: 399 WRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQ--SYFEEI 456
Query: 418 ALKFMKDSSQIASLQLLLRNYLSSTNDDYQVKQVILTSWIIQNYMNQL 465
ALKF+ ++ Q +L L+ L+S + + +LT+W+ + Y+++L
Sbjct: 457 ALKFL-EARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRL 503
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.134 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,256,954
Number of Sequences: 60738
Number of extensions: 2237781
Number of successful extensions: 6730
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6676
Number of HSP's gapped (non-prelim): 12
length of query: 924
length of database: 30,389,216
effective HSP length: 116
effective length of query: 808
effective length of database: 23,343,608
effective search space: 18861635264
effective search space used: 18861635264
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)