ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4379 good O KOG0710 Posttranslational modification, protein turnover, chaperones Molecular chaperone (small heat-shock protein Hsp26/Hsp42)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4379 1490667 1489483 -395
(395 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR171w [O] KOG0710 Molecular chaperone (small heat-shock protei... 280 2e-75
YBR072w [O] KOG0710 Molecular chaperone (small heat-shock protei... 65 2e-10
SPBC3E7.02c [O] KOG0710 Molecular chaperone (small heat-shock pr... 55 2e-07
>YDR171w [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 375
Score = 280 bits (717), Expect = 2e-75
Identities = 177/412 (42%), Positives = 230/412 (54%), Gaps = 63/412 (15%)
Query: 1 MSFYQPSLSLYDVLDALTNKAGARGDYEDNIYGGRQPQRRAANQG-------YDRYDPFA 53
MSFYQPSLSLYDVL+AL+N+ G RG + ++PQR + G + R+ P
Sbjct: 1 MSFYQPSLSLYDVLNALSNQTGQRGQ-QGYPRQPQRPQRYHPHYGQVHVGGHHPRHHPLY 59
Query: 54 ARRQGYVPGSYYYSVPN-ASYYQPVY-YTNEDAQPQRRPTHYHPHRTVRRTGAPTTASRR 111
+R G VP +YYY P A YY P Y Y +ED + + +
Sbjct: 60 SRYNG-VPNTYYYQFPGQAYYYSPEYGYDDEDGEEEDQD--------------------- 97
Query: 112 AVGPTSGDDVVGALLSALAGGNSDIFRGDADEESQRXXXXXX-XXXXXXXXXXXXXXXXX 170
+D+VG G + G D S+R
Sbjct: 98 -------EDMVGD------SGTTRQEDGGEDSNSRRYPSYYHCNTARNNRTNQQANSLND 144
Query: 171 XXXXXYSEEEKERNDQQIDEETAEQGEDASDEYNEDYNMHNNEEQQKLNKTTSSVDSEKD 230
E + +I+ T ++GE ++ +D + EE + NK EK
Sbjct: 145 LLTALIGVPPYEGTEPEIEANTEQEGEKGEEKDKKDKSEAPKEEAGETNK-------EKP 197
Query: 231 VNQKDK-----LAYEPSGFR-----SPVPAPLQVSNPELRLDLPFSPEVNVYDTPEQYVV 280
+NQ ++ LA + S F SP+P PLQVS PE R+DLPFSPEVNVYDT + YVV
Sbjct: 198 LNQLEESSRPPLAKKSSSFAHLQAPSPIPDPLQVSKPETRMDLPFSPEVNVYDTEDTYVV 257
Query: 281 VLALPGANSKEFKIDFHPSSHELLIKGAVDNKIGIDKKYVKISELKYGDFERSVKFPVLP 340
VLALPGANS+ F ID+HPSSHE+LIKG +++++GID+K++KI+ELKYG FER+VKFPVLP
Sbjct: 258 VLALPGANSRAFHIDYHPSSHEMLIKGKIEDRVGIDEKFLKITELKYGAFERTVKFPVLP 317
Query: 341 RIKDEEIKASYFNGLLQIKVPKIPQDANKPQPKKRIIIEDVPDEELVFETAP 392
RIKDEEIKA+Y NGLLQIKVPKI D KP+PKKRI IE++PDEEL FE P
Sbjct: 318 RIKDEEIKATYNNGLLQIKVPKIVNDTEKPKPKKRIAIEEIPDEELEFEENP 369
>YBR072w [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 214
Score = 64.7 bits (156), Expect = 2e-10
Identities = 35/109 (32%), Positives = 60/109 (54%), Gaps = 3/109 (2%)
Query: 269 VNVYDTPEQYVVVLALPGANSK-EFKIDFHPSSHELLIKGAVDNKIGIDKK-YVKISELK 326
V++ D Y + + +PG SK + I++H + +++L+ G + + + + K VK+ E
Sbjct: 95 VDILDHDNNYELKVVVPGVKSKKDIDIEYHQNKNQILVSGEIPSTLNEESKDKVKVKESS 154
Query: 327 YGDFERSVKFPVLPRIKDEEIKASYFNGLLQIKVPKI-PQDANKPQPKK 374
G F+R + P P + + IKA Y NG+L + VPK+ PQ K KK
Sbjct: 155 SGKFKRVITLPDYPGVDADNIKADYANGVLTLTVPKLKPQKDGKNHVKK 203
>SPBC3E7.02c [O] KOG0710 Molecular chaperone (small heat-shock protein
Hsp26/Hsp42)
Length = 143
Score = 54.7 bits (130), Expect = 2e-07
Identities = 39/128 (30%), Positives = 68/128 (52%), Gaps = 12/128 (9%)
Query: 256 NPELRLDLP--FSPEVNVYDTPEQYVVVLALPGANSKEFKIDFHPSSHELLIKGAVDN-- 311
+P L +P SP ++V++ + V + LPG ++ ++ H S +L I G V N
Sbjct: 24 SPRLNNQIPGELSPSIDVHEGKDTVSVDVELPGVKKEDVQV--HYDSGKLTISGEVVNER 81
Query: 312 KIGIDKKYVKISELKYGDFERSVKFPVLPRIKDEEIKASYFNGLLQIKVPKIPQDANKPQ 371
K + + SE ++G F R++ P +I + I+A++ NGLL + +PK+ K Q
Sbjct: 82 KNESTEGNQRWSERRFGSFSRTITIPA--KIDADRIEANFSNGLLTVTLPKV----EKSQ 135
Query: 372 PKKRIIIE 379
KK+I I+
Sbjct: 136 TKKQIAIK 143
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.311 0.133 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,093,829
Number of Sequences: 60738
Number of extensions: 1195901
Number of successful extensions: 6927
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 6920
Number of HSP's gapped (non-prelim): 5
length of query: 395
length of database: 30,389,216
effective HSP length: 108
effective length of query: 287
effective length of database: 23,829,512
effective search space: 6839069944
effective search space used: 6839069944
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)