ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4542 good U KOG3336 Intracellular trafficking, secretion, and vesicular transport Predicted member of the intramitochondrial sorting protein family
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4542 1548787 1548083 -235
(235 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR168c [U] KOG3336 Predicted member of the intramitochondrial s... 284 9e-77
YDR185c [U] KOG3336 Predicted member of the intramitochondrial s... 206 3e-53
SPBC36.10 [U] KOG3336 Predicted member of the intramitochondrial... 164 8e-41
7297061 [U] KOG3336 Predicted member of the intramitochondrial s... 115 5e-26
CE15853 [U] KOG3336 Predicted member of the intramitochondrial s... 114 1e-25
Hs7705610 [U] KOG3336 Predicted member of the intramitochondrial... 110 2e-24
At5g13070 [U] KOG3336 Predicted member of the intramitochondrial... 93 4e-19
Hs7019509 [U] KOG3337 Protein similar to predicted member of the... 83 4e-16
Hs22044072 [U] KOG3337 Protein similar to predicted member of th... 79 5e-15
YLR193c [U] KOG3337 Protein similar to predicted member of the i... 77 3e-14
SPAP8A3.10 [U] KOG3337 Protein similar to predicted member of th... 72 7e-13
Hs20543572 [U] KOG3336 Predicted member of the intramitochondria... 72 9e-13
Hs20473176 [U] KOG3337 Protein similar to predicted member of th... 69 6e-12
CE02936 [U] KOG3337 Protein similar to predicted member of the i... 58 1e-08
>YLR168c [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 230
Score = 284 bits (726), Expect = 9e-77
Identities = 138/230 (60%), Positives = 176/230 (76%), Gaps = 2/230 (0%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
M+LF N + F+Y WD +TAANWKKYPNE+STHV+AVDVLRREL GKVL +ERLIT KQ
Sbjct: 1 MKLFQNSYDFNYPWDQVTAANWKKYPNEISTHVIAVDVLRRELKDQGKVLVTERLITVKQ 60
Query: 61 SVPQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQ 120
VP+W+MM++GG+N+S+VREVSVVDLN+KSL +RSCNLT +LLKVYETV Y PHPDD
Sbjct: 61 GVPKWIMMMLGGTNMSHVREVSVVDLNKKSLTMRSCNLTMCNLLKVYETVTYSPHPDDSA 120
Query: 121 NRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLDESW-KQTDK 179
N+TLF+QEAQITAY + K+CNK+E+WSV RF ENA+KGK GFD+VL+V E+W K D
Sbjct: 121 NKTLFQQEAQITAYGSIRKLCNKMEDWSVQRFCENAKKGKMGFDAVLQVFSENWEKHVDD 180
Query: 180 IMDGLAEKVDETVVDIKKTTDILIKETEKKS-SKLASYYQYFSNAFRRSN 228
+ + L KV+ET+ D+K + L+K TE+ + L F +A+ N
Sbjct: 181 LSNQLVSKVNETMEDVKISAGTLLKGTERSGRTILQQNIDLFRDAYNHEN 230
>YDR185c [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 179
Score = 206 bits (523), Expect = 3e-53
Identities = 93/179 (51%), Positives = 127/179 (69%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
M+ F + FDY W+ +T ANW KYPN++STHV+AVDVLRREL G VL +ERLIT +Q
Sbjct: 1 MKSFQKSYEFDYPWEKVTTANWMKYPNKISTHVIAVDVLRRELKEHGDVLLTERLITIRQ 60
Query: 61 SVPQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQ 120
+ P W+ +LVG +N++YVREVS VD ++SL +RSCN+T +LK YETV+Y+PHP +P
Sbjct: 61 NTPHWMSILVGNTNLAYVREVSTVDRRDRSLTMRSCNMTFPHILKCYETVRYVPHPKNPS 120
Query: 121 NRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLDESWKQTDK 179
N TLFKQ+A+ + K+E W V RF +NA KGK GFDS+L + ++ WK ++
Sbjct: 121 NVTLFKQDAKFLSGVPTKTFSEKVENWGVKRFSDNAVKGKVGFDSILAMFNDIWKNANE 179
>SPBC36.10 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 184
Score = 164 bits (416), Expect = 8e-41
Identities = 83/177 (46%), Positives = 125/177 (69%), Gaps = 3/177 (1%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
M++F + H+F Y ++ ++AA+W+KYPNE +THV+AVD L R++ +G VL +ERLITC Q
Sbjct: 1 MKIFESCHLFQYPFEQVSAAHWQKYPNEHATHVIAVDTLDRKVLDNG-VLYTERLITCHQ 59
Query: 61 SVPQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQ 120
++P+W++ L+ G+ Y+RE S VDL ++L L + NLT S L+V ETV Y PHP+
Sbjct: 60 ALPRWILKLIDGAQDCYIRETSYVDLKARTLTLLTSNLTFSDRLRVDETVTYSPHPE--L 117
Query: 121 NRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLDESWKQT 177
T+F+QEA+I A A ++ N IE+WSV+ F + A +GK GF+SVL+ ++ S QT
Sbjct: 118 EATVFQQEARIEALACMKRLSNLIEQWSVDGFGKKASRGKEGFESVLEKINMSVFQT 174
>7297061 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 215
Score = 115 bits (288), Expect = 5e-26
Identities = 62/217 (28%), Positives = 118/217 (53%), Gaps = 9/217 (4%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
M+++ ++H+F++ W+ +T A W+KYPN ++ ++ DV+ R + + G VL + RL+ K
Sbjct: 1 MKIWTSEHIFNHPWETVTQAAWRKYPNPMTPSIIGTDVVERRV-VDG-VLHTHRLVQSKW 58
Query: 61 SVPQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQ 120
P+W L+G + + E S VD K ++L++ NLT + V E + Y PHP D
Sbjct: 59 YFPKWTHALIGTAKTCFASERSTVDPERKQMVLKTNNLTFCRNISVDEVLYYEPHPSD-A 117
Query: 121 NRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLDESWK----Q 176
++TL KQEA +T + + + +E+ + NA KG++G + V+ +++ K
Sbjct: 118 SKTLLKQEATVTVFG--VPLSHYMEDLLTSTISTNAGKGRQGLEWVIGLINTEVKGIARG 175
Query: 177 TDKIMDGLAEKVDETVVDIKKTTDILIKETEKKSSKL 213
TD+++ +DE +K+ D + + K + +
Sbjct: 176 TDELLHNTRRSIDEVTESARKSMDEISAQAAKAAKAM 212
>CE15853 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 209
Score = 114 bits (285), Expect = 1e-25
Identities = 63/199 (31%), Positives = 112/199 (55%), Gaps = 5/199 (2%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
MR++ ++H+FD+ W+ + A ++KYPN ++ + +DV+++ L+ +GK+LT ER+I
Sbjct: 1 MRIWSSEHIFDHEWETVAQAAFRKYPNPLNRSITGIDVVKQTLE-AGKILT-ERIIQSHF 58
Query: 61 SVPQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQ 120
S+P W L G S Y E +V+D K L + NL GSS L+V E + Y P +DP
Sbjct: 59 SIPSWATKLTGFSGTQYSHEYTVIDPTRKEFSLTTRNLNGSSFLRVDEKLTYTPAHEDP- 117
Query: 121 NRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLDESWKQTDKI 180
N+T+ KQ+ +T T + E+ ++ + +NA KG++G + V+ L + ++
Sbjct: 118 NKTILKQDVIVT--ITLPAFADYCEKTFLSIYSQNANKGRQGVEWVIDHLKKEYEAISTK 175
Query: 181 MDGLAEKVDETVVDIKKTT 199
+ ++ E V+ TT
Sbjct: 176 VSSEVHEMSEKVLRSFGTT 194
>Hs7705610 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 211
Score = 110 bits (275), Expect = 2e-24
Identities = 64/172 (37%), Positives = 105/172 (60%), Gaps = 5/172 (2%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
M+++ ++HVFD+ W+ +T A +KYPN ++ VV VDVL R +D SGK L S RL++ +
Sbjct: 1 MKIWTSEHVFDHPWETVTTAAMQKYPNPMNPSVVGVDVLDRHIDPSGK-LHSHRLLSTEW 59
Query: 61 SVPQWVMMLVGGSNI-SYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDP 119
+P V L+G + +YV+E SVVD EK++ L+S N++ ++++ V E + Y PHP DP
Sbjct: 60 GLPSIVKSLIGAARTKTYVQEHSVVDPVEKTMELKSTNISFTNMVSVDERLIYKPHPQDP 119
Query: 120 QNRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLD 171
+ +T+ QEA IT + + +E + NA KG+ + V+ L+
Sbjct: 120 E-KTVLTQEAIITVKG--VSLSSYLEGLMASTISSNASKGREAMEWVIHKLN 168
>At5g13070 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 183
Score = 92.8 bits (229), Expect = 4e-19
Identities = 47/177 (26%), Positives = 96/177 (53%), Gaps = 7/177 (3%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPN----EVSTHVVAVDVLRRELDISGKVLTSERLI 56
++ + +HV+ + W+ ++AA+W+K+ + + +H++ VD L R+LD L + R +
Sbjct: 2 VKAYRQEHVYKHPWERVSAASWRKFADPENKRILSHILEVDTLNRKLDTETGKLHTTRAL 61
Query: 57 TCKQSVPQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHP 116
T P W + + G +I + E +VVD +S+ L + N++ ++V E ++Y PHP
Sbjct: 62 TIHAPGP-WFLHRIIGQDICHCVESTVVDGKSRSMQLTTKNISLKKFIEVEERIRYDPHP 120
Query: 117 DDPQNRTLFKQEA--QITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLD 171
D+P T+ QE +I + + K+E+ +F +N+ KG+ + + K ++
Sbjct: 121 DNPSAWTVCSQETSIRIKPLSALASMAEKVEQKCAEKFMQNSAKGREVMERICKYME 177
>Hs7019509 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 219
Score = 82.8 bits (203), Expect = 4e-16
Identities = 62/217 (28%), Positives = 106/217 (48%), Gaps = 9/217 (4%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
++ F Q V SWD + AA W++YPN S HV+ D++ RE+ K+L S RL+T
Sbjct: 2 VKYFLGQSVLRSSWDQVFAAFWQRYPNPYSKHVLTEDIVHREVTPDQKLL-SRRLLTKTN 60
Query: 61 SVPQWVMML--VGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDD 118
+P+W L ++ YV E S+VD +++ + N+ + L+ V E Y + D+
Sbjct: 61 RMPRWAERLFPANVAHSVYVLEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDN 120
Query: 119 PQNRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVL--DESWKQ 176
T ++EA ++ ++ V ++E+ + RF N K +GF+ +L L + K
Sbjct: 121 -SGWTEIRREAWVS--SSLFGVSRAVQEFGLARFKSNVTKTMKGFEYILAKLQGEAPSKT 177
Query: 177 TDKIMDGLAEKVDET-VVDIKKTTDILIKETEKKSSK 212
+ EK ET + +K D+ K KK +
Sbjct: 178 LVETAKEAKEKAKETALAATEKAKDLASKAATKKQQQ 214
>Hs22044072 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 219
Score = 79.0 bits (193), Expect = 5e-15
Identities = 62/217 (28%), Positives = 104/217 (47%), Gaps = 9/217 (4%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
++ F Q V SWD + AA W++YPN S HV+ DV+ RE+ K+L S RL+T
Sbjct: 2 VKYFLGQSVQRSSWDQVFAAFWQRYPNPYSKHVLTEDVVHREVTPDQKLL-SGRLLTKTN 60
Query: 61 SVPQWVMMLVGGS--NISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDD 118
P W L + + Y+ E S+VD +++ + N+ + L+ V E Y + D+
Sbjct: 61 RTPCWAERLFPANVDHSVYILEDSIVDPQNQTMTTFTWNINHARLMVVEERCVYCVNSDN 120
Query: 119 PQNRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVL--DESWKQ 176
RT ++EA ++ ++ V ++E+ + F N K +GF+ +L L + K
Sbjct: 121 -SGRTEIRREAWVS--SSLFGVSRAVQEFGLAWFKSNVTKTMKGFEYILAKLQGEAPSKT 177
Query: 177 TDKIMDGLAEKVDET-VVDIKKTTDILIKETEKKSSK 212
+ EK ET + +K D+ K KK +
Sbjct: 178 LVETAKEAKEKAKETALAATEKAKDLASKAATKKQQQ 214
>YLR193c [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 175
Score = 76.6 bits (187), Expect = 3e-14
Identities = 46/171 (26%), Positives = 89/171 (51%), Gaps = 4/171 (2%)
Query: 3 LFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQSV 62
L + H+F + ++ A + +YPN S HV+++D + R +D G + T+ RL+ +
Sbjct: 4 LHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNVDQEGNLRTT-RLLKKSGKL 62
Query: 63 PQWVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQNR 122
P WV + G +++ EVSVV+ ++ + NL + ++KV E Y D +
Sbjct: 63 PTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTGIMKVEEYTTY--QFDSATSS 120
Query: 123 TLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLKVLDES 173
T+ + ++ + +K+E+WS +F EN +K + G V++ L+E+
Sbjct: 121 TIADSRVKFSSGFNM-GIKSKVEDWSRTKFDENVKKSRMGMAFVIQKLEEA 170
>SPAP8A3.10 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 171
Score = 72.0 bits (175), Expect = 7e-13
Identities = 46/156 (29%), Positives = 88/156 (55%), Gaps = 4/156 (2%)
Query: 13 SWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQSVPQWVMMLVGG 72
SW+ +++A +YPN S HVV+ DVL R +D G++ T ERL+ + +P+W L+
Sbjct: 14 SWNTVSSAWLTRYPNPYSLHVVSADVLERYVDDEGRLYT-ERLLVKQGRLPRWASDLL-N 71
Query: 73 SNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQNRTLFKQEAQIT 132
N SY+ E SV+D +++ L + NL +L+V E +++ ++ ++T+ A+
Sbjct: 72 VNKSYILERSVIDPSKQELKSETFNLDHVKILRVIEYSRFIQSSEN-CSKTIVDTIAKFV 130
Query: 133 AYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVLK 168
+ F + +++++S+ RF E +RG V++
Sbjct: 131 SPLRF-GLGRRVQKYSLKRFQEQLSSSRRGLLYVIQ 165
>Hs20543572 [U] KOG3336 Predicted member of the intramitochondrial sorting
protein family
Length = 143
Score = 71.6 bits (174), Expect = 9e-13
Identities = 49/135 (36%), Positives = 74/135 (54%), Gaps = 5/135 (3%)
Query: 26 PNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQSVPQWVMMLVGGSNI-SYVREVSVV 84
P VV VDVL R +D SGK L S RL++ + +P V L+G + + +YV+E SVV
Sbjct: 13 PKPYEPTVVGVDVLDRHVDPSGK-LHSHRLLSTEWGLPSIVKSLIGAARMKTYVQEHSVV 71
Query: 85 DLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQNRTLFKQEAQITAYATFTKVCNKI 144
D EK++ L+S N++ + ++ E + Y P P DP+ +T+ QEA IT + +
Sbjct: 72 DPVEKTMKLKSTNISFTDMVSADERLIYKPRPRDPE-KTILTQEAIITMKG--VSLSSYF 128
Query: 145 EEWSVNRFHENAEKG 159
EE + N KG
Sbjct: 129 EELMASMISSNTSKG 143
>Hs20473176 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 250
Score = 68.9 bits (167), Expect = 6e-12
Identities = 44/169 (26%), Positives = 85/169 (50%), Gaps = 6/169 (3%)
Query: 1 MRLFHNQHVFDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQ 60
++ F Q V SWD + A W +YPN S HV+ D + RE+ K+L S++L+T
Sbjct: 2 VKYFLGQGVLQSSWDQVFTAFWHQYPNPYSKHVLTEDTVHREVTADQKLL-SQQLLTKTN 60
Query: 61 SVPQWVMMLVGGSNIS--YVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDD 118
+ W L + + Y+ E S+VD +++ + N+ + L+ V + Y + ++
Sbjct: 61 RMSHWAKQLFPANVLHSVYILEDSIVDPQNQTMTTFTWNINHARLMVVEKQCVYCMNSNN 120
Query: 119 PQNRTLFKQEAQITAYATFTKVCNKIEEWSVNRFHENAEKGKRGFDSVL 167
T ++EA ++ ++ V ++E+ + +F N K +GF+ +L
Sbjct: 121 -SGWTEIRREAWVS--SSLFGVSRSVQEFGLAQFKSNVTKTMKGFEYIL 166
>CE02936 [U] KOG3337 Protein similar to predicted member of the
intramitochondrial sorting protein family
Length = 210
Score = 58.2 bits (139), Expect = 1e-08
Identities = 34/117 (29%), Positives = 63/117 (53%), Gaps = 8/117 (6%)
Query: 10 FDYSWDYITAANWKKYPNEVSTHVVAVDVLRRELDISGKVLTSERLITCKQS-----VPQ 64
F YS+D + +A W +YPN + H+++ DVL R+ I+ + +++LI + S VP+
Sbjct: 11 FPYSFDEVASAFWDRYPNSHAKHIISEDVLERQ--ITDNTIVTKKLIVKQGSSILKRVPR 68
Query: 65 WVMMLVGGSNISYVREVSVVDLNEKSLILRSCNLTGSSLLKVYETVKYMPHPDDPQN 121
W+ + + + E SV D K L+ + N++ SL +++E Y D+ Q+
Sbjct: 69 WISRMTDIQVVPVIEE-SVYDKVSKKLVTYTRNVSHISLFQLHERCIYKSSEDNQQH 124
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.131 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,869,244
Number of Sequences: 60738
Number of extensions: 545249
Number of successful extensions: 1901
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1879
Number of HSP's gapped (non-prelim): 14
length of query: 235
length of database: 30,389,216
effective HSP length: 103
effective length of query: 132
effective length of database: 24,133,202
effective search space: 3185582664
effective search space used: 3185582664
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)