ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4596 good YU KOG0845 Intracellular trafficking, secretion, and vesicular transport Nuclear pore complex, Nup98 component (sc Nup145/Nup100/Nup116)
r_klactIV4596 good YU KOG0845 Nuclear structure Nuclear pore complex, Nup98 component (sc Nup145/Nup100/Nup116)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4596 1567220 1565445 -592
(592 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR192c [YU] KOG0845 Nuclear pore complex Nup98 component (sc Nu... 72 2e-12
At1g59660 [YU] KOG0845 Nuclear pore complex Nup98 component (sc ... 59 2e-08
Hs4826874 [YU] KOG3630 Nuclear pore complex Nup214/CAN component 55 2e-07
At1g10390 [YU] KOG0845 Nuclear pore complex Nup98 component (sc ... 55 4e-07
>YDR192c [YU] KOG0845 Nuclear pore complex Nup98 component (sc
Nup145/Nup100/Nup116)
Length = 430
Score = 72.4 bits (176), Expect = 2e-12
Identities = 95/375 (25%), Positives = 131/375 (34%), Gaps = 72/375 (19%)
Query: 228 FGQSAFGSGNMTNGLASSSDSILGKPAXXXXXXXXXXXXXXXXXXQLQNNAGKN--AQNS 285
FG AFGS N S+ S G P+ N GK+ + S
Sbjct: 92 FGAPAFGSSAPINVNPPSTTSAFGAPSFGSTGFGAMAAT--------SNPFGKSPGSMGS 143
Query: 286 NAQTPPFGTNVSQSPAQTNXXXXXXXXXXXXKPAFSNQTSLQPTNNTTTGMASSSAFG-- 343
P FG N + P+ + + SN ++ +NT + ++S FG
Sbjct: 144 AFGQPAFGANKTAIPSSS--------------VSNSNNSAFGAASNTP--LTTTSPFGSL 187
Query: 344 QSSNEPAFRTTSGAFGSPSFGQSAFGQPAFGNXXXXXXXXXXXXXXXXXXXXFGQSTFGQ 403
Q + +TS AFG P+FG + Q FG FG ++ +
Sbjct: 188 QQNASQNASSTSSAFGKPTFGAATNTQSPFG---TIQNTSTSSGTGVSPFGTFGTNSNNK 244
Query: 404 SAFGQPTFGKPSGTGQSAFGSTTSGQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXNSAPA 463
S F G +G G S FG+TTS N +P
Sbjct: 245 SPFSNLQSG--AGAGSSPFGTTTS--------------KANNNNNVGSSAFGTTNNQSPF 288
Query: 464 SSGNVSTATTSSMQNTSPFGGVN------------------NTINSQNPSPFGQQTAPNA 505
S G+ T ++S N + G N ++ NPS FG QT PN
Sbjct: 289 SGGSGGTFGSASNLNKNTNGNFQSSFGNKGFSFGITPQNDANKVSQSNPS-FG-QTMPNT 346
Query: 506 SSAQTPFQSFGSVPAATPFGALAXXXXXXXXXXXXXXGLPLFVQGL-DDKSETTAMSDLS 564
P S S AT FG G FVQGL +K ++DL+
Sbjct: 347 D----PNISLKSNGNATSFGFGQQQMNATNVNANTATGKIRFVQGLSSEKDGILELADLA 402
Query: 565 ERTIAIFKDTKFELG 579
E T+ IF+ KFELG
Sbjct: 403 EETLKIFRANKFELG 417
>At1g59660 [YU] KOG0845 Nuclear pore complex Nup98 component (sc
Nup145/Nup100/Nup116)
Length = 997
Score = 59.3 bits (142), Expect = 2e-08
Identities = 80/296 (27%), Positives = 100/296 (33%), Gaps = 50/296 (16%)
Query: 274 LQNNAGKNAQNSNAQTPPFGTNVSQSPAQTNXXXXXXXXXXXXKPAFSNQTSLQPTNNTT 333
L NA N+ T PFGT+ + P S+ P
Sbjct: 26 LFGQTNNNASNNPFATKPFGTSTPFGAQTGSSMFGGTSTGVFGAPQTSSPFGASP----Q 81
Query: 334 TGMASSSAFGQSSNEPAFRTTSGAFGSPS-FGQSAFG----------------QPAFGNX 376
+S+ AFG SS P+F +++ FG S FGQ +FG QPAFGN
Sbjct: 82 AFGSSTQAFGASST-PSFGSSNSPFGGTSTFGQKSFGLSTPQSSPFGSTTQQSQPAFGNS 140
Query: 377 XXXXX----XXXXXXXXXXXXXXFGQS-TFGQSAFGQPTFGKPSGT---GQSAFGSTTSG 428
FG S T G A P FG + T G S G S
Sbjct: 141 TFGSSTPFGASTTPAFGASSTPAFGVSNTSGFGATNTPGFGATNTTGFGGSSTPGFGASS 200
Query: 429 QPPXXXXXXXXXXXXXXXXXXXXXXXXXXXNSAPA--SSGNV--------------STAT 472
P + APA SSGN S+
Sbjct: 201 TPAFGSTNTPAFGASSTPLFGSSSSPAFGASPAPAFGSSGNAFGNNTFSSGGAFGSSSTP 260
Query: 473 TSSMQNTSPFGGVNNTINSQNPSP-FGQQTAPNASSAQTPFQ-SFGSVPAATPFGA 526
T NTS FG ++ + SP FGQ T+ SS+ Q S GS P +PFGA
Sbjct: 261 TFGASNTSAFGASSSPSFNFGSSPAFGQSTSAFGSSSFGSTQSSLGSTP--SPFGA 314
Score = 58.2 bits (139), Expect = 4e-08
Identities = 61/252 (24%), Positives = 91/252 (35%), Gaps = 30/252 (11%)
Query: 277 NAGKNAQNSNAQTPPFGTNVSQSPAQTNXXXXXXXXXXXXKPAFSNQTSLQPTNNTTTGM 336
++ N ++ + PFGT QTN KP ++ T ++ G
Sbjct: 4 SSNNNPFGQSSISSPFGTQTHSLFGQTNNNASNNPFAT--KPFGTSTPFGAQTGSSMFGG 61
Query: 337 ASSSAFGQSSNEPAFRTTSGAFGSPSFGQSAFGQPAFGNXXXXXXXXXXXXXXXXXXXXF 396
S+ FG F + AFGS + A P+FG+
Sbjct: 62 TSTGVFGAPQTSSPFGASPQAFGSSTQAFGASSTPSFGSSNSPFG--------------- 106
Query: 397 GQSTFGQSAFGQPT-----FGKPSGTGQSAFGSTTSG-QPPXXXXXXXXXXXXXXXXXXX 450
G STFGQ +FG T FG + Q AFG++T G P
Sbjct: 107 GTSTFGQKSFGLSTPQSSPFGSTTQQSQPAFGNSTFGSSTPFGASTTPAFGASSTPAFGV 166
Query: 451 XXXXXXXXNSAPASSGNVSTATTSSMQNTSPFGGVNNT--INSQNPSPFGQQTAPNASSA 508
+ P G +T TT +++P G ++T S N FG + P S+
Sbjct: 167 SNTSGFGATNTP---GFGATNTTGFGGSSTPGFGASSTPAFGSTNTPAFGASSTPLFGSS 223
Query: 509 QTPFQSFGSVPA 520
+P +FG+ PA
Sbjct: 224 SSP--AFGASPA 233
>Hs4826874 [YU] KOG3630 Nuclear pore complex Nup214/CAN component
Length = 2090
Score = 55.5 bits (132), Expect = 2e-07
Identities = 61/229 (26%), Positives = 82/229 (35%), Gaps = 40/229 (17%)
Query: 318 PAFSNQTSL-QPTNNTTTGMASSSAFGQ--SSNEPAF------RTTSGAFGSPSFGQSAF 368
PA S+ ++ Q TNNT T +++ FGQ +S P+ T S A +P S F
Sbjct: 1649 PAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGF 1708
Query: 369 GQPAFGNXXXXXXXXXXXXXXXXXXXXFGQSTFGQ-SAFGQPTFGKPS--GTGQSAFGST 425
PAFG FGQ+TFGQ S FGQ S Q F S
Sbjct: 1709 SSPAFG---------------TTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSV 1753
Query: 426 TS-GQPPXXXXXXXXXXXXXXXXXXXXXXXXXXXNSAPASSGNVSTATTSSMQNTSPFGG 484
+ GQP S+P + G + + + SP G
Sbjct: 1754 PAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFG 1813
Query: 485 VNNTINSQNPSPFGQQTAPNASSAQTPF-----QSFGSVPAATPFGALA 528
Q S FG +A ++A + F FGS + FG A
Sbjct: 1814 -------QGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAA 1855
>At1g10390 [YU] KOG0845 Nuclear pore complex Nup98 component (sc
Nup145/Nup100/Nup116)
Length = 1043
Score = 54.7 bits (130), Expect = 4e-07
Identities = 87/350 (24%), Positives = 109/350 (30%), Gaps = 62/350 (17%)
Query: 228 FGQSAFGSGN---MTNGLASSSDSILGKPAXXXXXXXXXXXXXXXXXXQLQNNAGKNAQN 284
FG ++FGS TN A + S A A +
Sbjct: 135 FGNTSFGSSTPFGATNTPAFGAPSTPSFGATSTPSFGASSTPAFGATNTPAFGASNSPSF 194
Query: 285 SNAQTPPFGTNVSQSPAQTNXXXXXXXXXXXXKPAFSNQTSLQPTNNTTTGMASSSAFGQ 344
TP FG + + + T SN + + G + + AFG
Sbjct: 195 GATNTPAFGASPTPAFGSTGTTFGNTGFGSGGAFGASNTPAFGASGTPAFGASGTPAFGA 254
Query: 345 SSNEPAFRTTSGAFGSPSFGQSAFGQPAFGNXXX-XXXXXXXXXXXXXXXXXFGQST--F 401
SS PAF GA +P+FG S+ PAFG FGQST F
Sbjct: 255 SST-PAF----GASSTPAFGASS--TPAFGGSSTPSFGASNTSSFSFGSSPAFGQSTSAF 307
Query: 402 GQSAFGQ--------------PTFGKPSGTGQSAFGSTTSGQ------PPXXXXXXXXXX 441
G SAFG PTFG SG GQS FG G P
Sbjct: 308 GSSAFGSTPSPFGGQGAQASTPTFG-GSGFGQSTFGGQQGGSRAVPYAPTVEADTGTGTQ 366
Query: 442 XXXXXXXXXXXXXXXXXN------------------SAPASSGNVSTATTSSMQNT-SPF 482
N A S GN + S N SP
Sbjct: 367 PAGKLESISAMPAYKEKNYEELRWEDYQRGDKGGPLPAGQSPGNAGFGISPSQPNPFSPS 426
Query: 483 GGVNNTINSQNP-SPFGQQTAPNASSAQTP---FQSFGSVPA---ATPFG 525
T S NP +PF T+ N + QTP SFG+ + ++PFG
Sbjct: 427 PAFGQT--SANPTNPFSSSTSTNPFAPQTPTIASSSFGTATSNFGSSPFG 474
Score = 50.8 bits (120), Expect = 6e-06
Identities = 57/216 (26%), Positives = 75/216 (34%), Gaps = 28/216 (12%)
Query: 323 QTSLQPTNNT---TTGMASSSAFGQSSNEPAFRTTS-GAFGSPSFGQSAFGQPAFGNXXX 378
QTS +NN T +S+ F S F +TS G FG+P P FG
Sbjct: 25 QTSNTSSNNPFAPATPFGTSAPFAAQSGSSIFGSTSTGVFGAPQTSSPFASTPTFG---- 80
Query: 379 XXXXXXXXXXXXXXXXXFGQST--FGQSAFGQPTFGKPSGTGQSAFGSTTSGQPPXXXXX 436
FG ST FG S P FG SG GQ G +T P
Sbjct: 81 -----------ASSSPAFGNSTPAFGASPASSP-FGGSSGFGQKPLGFSTPQSNPFGNST 128
Query: 437 XXXXXXXXXXXXXXXXXXXXXXN---SAPASSGNVSTATTSSMQNTSPFGGVNNT--INS 491
AP++ +T+T S +++P G NT +
Sbjct: 129 QQSQPAFGNTSFGSSTPFGATNTPAFGAPSTPSFGATSTPSFGASSTPAFGATNTPAFGA 188
Query: 492 QNPSPFGQQTAPNASSAQTP-FQSFGSVPAATPFGA 526
N FG P ++ TP F S G+ T FG+
Sbjct: 189 SNSPSFGATNTPAFGASPTPAFGSTGTTFGNTGFGS 224
Score = 50.4 bits (119), Expect = 7e-06
Identities = 82/333 (24%), Positives = 108/333 (31%), Gaps = 54/333 (16%)
Query: 233 FGSGNMTNGLASSSDSILGKPAXXXXXXXXXXXXXXXXXXQLQNNAGKNAQNSNAQTPPF 292
FGS N SS S G + +A AQ+ ++
Sbjct: 2 FGSSNPFG--QSSGTSPFGSQSLFGQTSNTSSNNPFAPATPFGTSAPFAAQSGSSIFGST 59
Query: 293 GTNVSQSPAQTNXXXXXXXXXXXXKPAFSNQTSLQPTNNTTTGMASSSAFGQ-------- 344
T V +P ++ PAF N T + ++ SS FGQ
Sbjct: 60 STGVFGAPQTSSPFASTPTFGASSSPAFGNSTPAFGASPASSPFGGSSGFGQKPLGFSTP 119
Query: 345 ---------SSNEPAFRTTS-------GAFGSPSFGQSAFGQPAFG-NXXXXXXXXXXXX 387
++PAF TS GA +P+FG A P+FG
Sbjct: 120 QSNPFGNSTQQSQPAFGNTSFGSSTPFGATNTPAFG--APSTPSFGATSTPSFGASSTPA 177
Query: 388 XXXXXXXXFGQS---TFGQS---AFG---QPTFGKPSGT-GQSAFGS----TTSGQPPXX 433
FG S +FG + AFG P FG T G + FGS S P
Sbjct: 178 FGATNTPAFGASNSPSFGATNTPAFGASPTPAFGSTGTTFGNTGFGSGGAFGASNTPAFG 237
Query: 434 XXXXXXXXXXXXXXXXXXXXXXXXXNSAPASSGNVSTATTSSMQNTSPFGGVNNTINSQN 493
+S PA + + A S +T FG N + S
Sbjct: 238 ASGTPAFGASGTPAFGASSTPAFGASSTPAFGASSTPAFGGS--STPSFGASNTSSFSFG 295
Query: 494 PSP-FGQQTAPNASSAQTPFQSFGSVPAATPFG 525
SP FGQ T+ SSA FGS P +PFG
Sbjct: 296 SSPAFGQSTSAFGSSA------FGSTP--SPFG 320
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.309 0.125 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,557,078
Number of Sequences: 60738
Number of extensions: 1062651
Number of successful extensions: 3744
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 3603
Number of HSP's gapped (non-prelim): 36
length of query: 592
length of database: 30,389,216
effective HSP length: 112
effective length of query: 480
effective length of database: 23,586,560
effective search space: 11321548800
effective search space used: 11321548800
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)