ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV4712   suspect: LH   U     KOG2395  Intracellular trafficking, secretion, and vesicular transport  Protein involved in vacuole import and degradation

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV4712 1612193 1611327 -289 
         (289 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YNL212w [U] KOG2395 Protein involved in vacuole import and degra...   114   2e-25

>YNL212w [U] KOG2395 Protein involved in vacuole import and degradation
          Length = 782

 Score =  114 bits (284), Expect = 2e-25
 Identities = 62/201 (30%), Positives = 111/201 (54%), Gaps = 5/201 (2%)

Query: 1   MNFIRTFFDSNSNEELLSISAGQFLLLRSFKSPKSSVEYIFSDAMICVKRRGDYDYILHV 60
           MN ++ F +S +  EL++I +GQF LLRS  SPK+++E I+++A + V++ G +DY L V
Sbjct: 1   MNILKKFMESGNKPELITIPSGQFNLLRSKNSPKAALECIYNNATLSVRKIGKFDYELAV 60

Query: 61  ERQXXXXXXXXXXXXXXLVPDEMSLLSATS-KKDDTWAFKIEEDLQFVKSWNAERHSVFI 119
            R                  D +S+LS  S KK++ W+ +I + + F K+W+ + +   +
Sbjct: 61  YRVEDDSEGGTGDEAENFEDDTISVLSTQSKKKEEEWSVEISDKIMFHKTWDKQGNVALV 120

Query: 120 WNNTKGDDGDQ-FEYIIDDTIPLLEIEKFYKLVKHCTFEAKYRKPSDQATKQELLEFMKP 178
           W N +GD+ D+  ++++   +   ++E+F + V  C FE + +K S  A+  +L E    
Sbjct: 121 WENLRGDEQDEKVQFVVAADVSFSDVEQFIQTVYRCQFEVRNKKSSLTASADDLKEI--- 177

Query: 179 EELDDSPFFSDEYNELRTDSD 199
           E      F  D+ +EL + SD
Sbjct: 178 EHRSTRLFVQDDDDELDSSSD 198


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.315    0.133    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,236,093
Number of Sequences: 60738
Number of extensions: 629126
Number of successful extensions: 1648
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1644
Number of HSP's gapped (non-prelim): 1
length of query: 289
length of database: 30,389,216
effective HSP length: 105
effective length of query: 184
effective length of database: 24,011,726
effective search space: 4418157584
effective search space used: 4418157584
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)