ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV4766   suspect: LH   T     KOG4747  Signal transduction mechanisms                                 Two-component phosphorelay intermediate involved in MAP kinase cascade regulation

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV4766 1628572  1628976 135  
         (135 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL235c [T] KOG4747 Two-component phosphorelay intermediate invo...   135   2e-32
SPBC725.02 [T] KOG4747 Two-component phosphorelay intermediate i...    87   6e-18

>YDL235c [T] KOG4747 Two-component phosphorelay intermediate involved in MAP
           kinase cascade regulation
          Length = 167

 Score =  135 bits (340), Expect = 2e-32
 Identities = 74/162 (45%), Positives = 99/162 (60%), Gaps = 35/162 (21%)

Query: 7   IPDKVINWDILNEIVSMDEDDAGFSQSLLIQFFEQATSTFNQIEQHIKTDKNLDQLGQLG 66
           IP ++INW ILNEI+SMD+DD+ FS+ L+IQF +QA +TF Q+++ +  +KNL +L  LG
Sbjct: 4   IPSEIINWTILNEIISMDDDDSDFSKGLIIQFIDQAQTTFAQMQRQLDGEKNLTELDNLG 63

Query: 67  HFLKGSSASLGLQRIAWVCERIQNYGQKRE--------------------GSGITDDN-- 104
           HFLKGSSA+LGLQRIAWVCERIQN G+K E                    G  I +D+  
Sbjct: 64  HFLKGSSAALGLQRIAWVCERIQNLGRKMEHFFPNKTELVNTLSDKSIINGINIDEDDEE 123

Query: 105 -------------YIQLIQNSLDLARKEFDSAKSELGQYYKT 133
                        Y+ LI  +L+ +R EF  A+ EL +YY T
Sbjct: 124 IKIQVDDKDENSIYLILIAKALNQSRLEFKLARIELSKYYNT 165


>SPBC725.02 [T] KOG4747 Two-component phosphorelay intermediate involved in MAP
           kinase cascade regulation
          Length = 295

 Score = 87.0 bits (214), Expect = 6e-18
 Identities = 50/132 (37%), Positives = 83/132 (62%), Gaps = 7/132 (5%)

Query: 7   IPDK-VINWDILNEIVSMDEDDAG-FSQSLLIQFFEQATSTFNQIEQHIKTDKNLDQLGQ 64
           I DK +I+  + ++++ MD+DD   FS+S++  +FEQA +T   +++ ++  K+L +L  
Sbjct: 160 IQDKQLIDHSVFDQLLEMDDDDEHEFSKSIVWNYFEQAETTIADLQKALEA-KDLKKLSS 218

Query: 65  LGHFLKGSSASLGLQRIAWVCERIQNYGQKREGSGI----TDDNYIQLIQNSLDLARKEF 120
           LGHFLKGSSA LGL ++  VCERIQNYG  R   G+    +++  + LI  SL +    +
Sbjct: 219 LGHFLKGSSAVLGLTKMRKVCERIQNYGSLRSRDGVMKLPSEEIALDLISKSLSVVNDFY 278

Query: 121 DSAKSELGQYYK 132
             A++ L  +Y+
Sbjct: 279 KDARAYLLDFYE 290


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,788,813
Number of Sequences: 60738
Number of extensions: 286023
Number of successful extensions: 792
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 788
Number of HSP's gapped (non-prelim): 3
length of query: 135
length of database: 30,389,216
effective HSP length: 95
effective length of query: 40
effective length of database: 24,619,106
effective search space: 984764240
effective search space used: 984764240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)