ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4807 good ZU KOG2674 Cytoskeleton Cysteine protease required for autophagy - Apg4p/Aut2p
r_klactIV4807 good ZU KOG2674 Intracellular trafficking, secretion, and vesicular transport Cysteine protease required for autophagy - Apg4p/Aut2p
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4807 1647682 1646333 -450
(450 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL223w [ZU] KOG2674 Cysteine protease required for autophagy - ... 350 3e-96
SPAC19B12.08 [ZU] KOG2674 Cysteine protease required for autopha... 135 1e-31
7300008 [ZU] KOG2674 Cysteine protease required for autophagy - ... 128 2e-29
At3g59950 [ZU] KOG2674 Cysteine protease required for autophagy ... 110 3e-24
7296129 [ZU] KOG2674 Cysteine protease required for autophagy - ... 110 4e-24
Hs16418417 [ZU] KOG2674 Cysteine protease required for autophagy... 110 6e-24
Hs18378733 [ZU] KOG2674 Cysteine protease required for autophagy... 107 4e-23
CE24740 [ZU] KOG2674 Cysteine protease required for autophagy - ... 105 1e-22
Hs21361868 [ZU] KOG2674 Cysteine protease required for autophagy... 104 2e-22
Hs14249578 [ZU] KOG2674 Cysteine protease required for autophagy... 96 8e-20
At2g44140 [ZU] KOG2674 Cysteine protease required for autophagy ... 89 1e-17
CE23129 [ZU] KOG2674 Cysteine protease required for autophagy - ... 60 5e-09
HsM14249640 [ZU] KOG2674 Cysteine protease required for autophag... 55 2e-07
>YNL223w [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 506
Score = 350 bits (897), Expect = 3e-96
Identities = 187/417 (44%), Positives = 258/417 (61%), Gaps = 20/417 (4%)
Query: 45 DSALESLIN--IVSLNPGLLSDVHSRVFFTYRTQFTPIRRNENGPSPINFTLFFRDNPIN 102
D L SL I S L DV SRV FTYRT+F PI R +GPSP++ L R NPI+
Sbjct: 80 DGFLSSLFGREISSYTKEFLLDVQSRVNFTYRTRFVPIARAPDGPSPLSLNLLVRTNPIS 139
Query: 103 TLENALTDPDSFYSDIGWGCMIRTGQALLANAIQRVKLAREFRINASRIDDNELNLIRWF 162
T+E+ + +PD F +DIGWGCMIRTGQ+LL NA+Q + L R+FR+N + + E + WF
Sbjct: 140 TIEDYIANPDCFNTDIGWGCMIRTGQSLLGNALQILHLGRDFRVNGNESLERESKFVNWF 199
Query: 163 QDDVKYPLSLHNFVKAEEKISGMKPGQWFGPSATARSIKTLIEGFPLCGIKNCIISTQSA 222
D + P SLHNFV A ++S +PG+WFGP+ATARSI++LI GFP CGI +CI+S S
Sbjct: 200 NDTPEAPFSLHNFVSAGTELSDKRPGEWFGPAATARSIQSLIYGFPECGIDDCIVSVSSG 259
Query: 223 DIYEDEVTRIFHKDRDANLLLLFAVRLGVDKINSLYWKDIFKILSSPYSVGIAGGKPSSS 282
DIYE+EV ++F ++ ++ +L L V+LG++ +N Y + I ILSS SVGIAGG+PSSS
Sbjct: 260 DIYENEVEKVFAENPNSRILFLLGVKLGINAVNESYRESICGILSSTQSVGIAGGRPSSS 319
Query: 283 LYFFGYQNENLFYLDPHNTQQSSLMMDDLEFYRSCHGHKFNKLHISETDPSMLLGMLISG 342
LYFFGYQ + DPH Q + ++D F SCH KF KL +SE DPSML+G+LI G
Sbjct: 320 LYFFGYQGNEFLHFDPHIPQPA---VED-SFVESCHTSKFGKLQLSEMDPSMLIGILIKG 375
Query: 343 KNEWDQFHTYFRDSQIIQFLTTRPENDLYQSANMSVGSDSIHSLESDIQDT-------GE 395
+ +W Q+ +S II L R ++ + V S S +S++ D + G+
Sbjct: 376 EKDWQQWKLEVAESAIINVLAKRMDDFDVSCSMDDVESVSSNSMKKDASNNENLGVLEGD 435
Query: 396 YVDVGTLVSGKQMSSPQPKDEEFEDVKCKSQRIIVCEDP-------ADPEVEQVLVE 445
YVD+G + + + + F+D+ CK Q+I+V + D EVE VLVE
Sbjct: 436 YVDIGAIFPHTTNTEDVDEYDCFQDIHCKKQKIVVMGNTHTVNANLTDYEVEGVLVE 492
>SPAC19B12.08 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 320
Score = 135 bits (341), Expect = 1e-31
Identities = 80/268 (29%), Positives = 129/268 (47%), Gaps = 23/268 (8%)
Query: 103 TLENALTDPDSFYSDIGWGCMIRTGQALLANAIQRVKLAREFRINASRIDDNELNLIRWF 162
T + + ++ SD GWGCMIR+ Q LLAN ++ ++ + ++ F
Sbjct: 55 TYRSGIEGLENMTSDTGWGCMIRSTQTLLANCLRICYPEKQLK-----------EILALF 103
Query: 163 QDDVKYPLSLHNFVKAEEKISGMKPGQWFGPSATARSIKTLIEGFPLCGIKNCIISTQSA 222
D+ P S+H FV + + + PGQWFGP+ + + L + P + + +A
Sbjct: 104 ADEPSAPFSIHQFVTMGKTLCDINPGQWFGPTTSCSCVARLSDQNPDVPLHVYVARNGNA 163
Query: 223 DIYEDEVTRIFHKDRDANLLLLFAVRLGVDKINSLYWKDIFKILSSPYSVGIAGGKPSSS 282
IY D+++++ +LLL RLG+D IN Y+ + ++ VGI GG+P S+
Sbjct: 164 -IYRDQLSKVSFP-----VLLLIPTRLGIDSINESYYDQLLQVFEIRSFVGITGGRPRSA 217
Query: 283 LYFFGYQNENLFYLDPHNTQQSSLMMDDLEFYRSCHGHKFNKLHISETDPSMLLGMLISG 342
YF+ QN+ FYLDPH T + + H ++ I + DP M+ G LI
Sbjct: 218 HYFYARQNQYFFYLDPHCTHFAHTTTQPAS-EETFHSATLRRVAIQDLDPCMIFGFLIRD 276
Query: 343 KNEWDQFHT---YFRDSQIIQFLTTRPE 367
+ EW F YF D I+Q + P+
Sbjct: 277 EEEWHSFEANQKYFAD--IVQIFDSEPQ 302
>7300008 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 702
Score = 128 bits (322), Expect = 2e-29
Identities = 93/328 (28%), Positives = 146/328 (44%), Gaps = 68/328 (20%)
Query: 60 GLLSDVHSRVFFTYRTQFTPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIG 119
G D +SR++ TYR +F PI N + SD G
Sbjct: 299 GFRRDFYSRIWMTYRREF-PIMNGSN----------------------------YTSDCG 329
Query: 120 WGCMIRTGQALLANAIQRVKLAREFRINA-----SRIDDN-ELNLIRWFQDDVKY--PLS 171
WGCM+R+GQ L A + L R +R ++ S +DN +++WF D P S
Sbjct: 330 WGCMLRSGQMLFAQGLICHFLGRSWRYDSESQLHSTYEDNMHKKIVKWFGDSSSKSSPFS 389
Query: 172 LHNFVKAEEKISGMKPGQWFGPSATARSIKTLIE----------GFPLCGIKNCIISTQS 221
+H V+ E + G KPG W+GP++ + +K +E + K+C I Q
Sbjct: 390 IHALVRLGEHL-GKKPGDWYGPASVSYLLKHALEHAAQENADFDNISVYVAKDCTIYLQD 448
Query: 222 ADIY--------------------EDEVTRIFHKDRDANLLLLFAVRLGVDKINSLYWKD 261
+ + E T+ + +L++L +RLG DK+N +Y
Sbjct: 449 IEDQCSIPEPAPKPHVPWQQAKRPQAETTKTEQQQHWKSLIVLIPLRLGSDKLNPVYAHC 508
Query: 262 IFKILSSPYSVGIAGGKPSSSLYFFGYQNENLFYLDPHNTQQSSLMMDDLEFYRSCHGHK 321
+ +LS+ + +GI GGKP SLYF G+Q + L +LDPH Q+ + + S H
Sbjct: 509 LKLLLSTEHCLGILGGKPKHSLYFVGFQEDRLIHLDPHYCQEMVDVNQENFSLHSFHCKS 568
Query: 322 FNKLHISETDPSMLLGMLISGKNEWDQF 349
KL S+ DPS +G + K+++D F
Sbjct: 569 PRKLKASKMDPSCCIGFYCATKSDFDNF 596
>At3g59950 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 477
Score = 110 bits (276), Expect = 3e-24
Identities = 75/262 (28%), Positives = 125/262 (47%), Gaps = 28/262 (10%)
Query: 113 SFYSDIGWGCMIRTGQALLANAIQRVKLAREFRINASR-IDDNELNLIRWFQDDVKYPLS 171
++ SD+ WGCM+R+GQ L A A+ +L R +R S D+ L ++ F D S
Sbjct: 164 TYTSDVNWGCMLRSGQMLFAQALLFQRLGRSWRKKDSEPADEKYLEILELFGDTEASAFS 223
Query: 172 LHNFVKAEEKISGMKPGQWFGPSATARSIKTLI-----------EGFPLCGIKNCIISTQ 220
+HN + A E G+ G W GP A RS ++L + F + + + ++
Sbjct: 224 IHNLILAGESY-GLAAGSWVGPYAVCRSWESLARKNKEETDDKHKSFSMA--VHIVSGSE 280
Query: 221 SAD------IYEDEVTRIFHKDRDAN-----LLLLFAVRLGVDKINSLYWKDIFKILSSP 269
+ + ++VT+ + + +LLL + LG+D++N Y + + P
Sbjct: 281 DGERGGAPILCIEDVTKTCLEFSEGETEWPPILLLVPLVLGLDRVNPRYIPSLIATFTFP 340
Query: 270 YSVGIAGGKPSSSLYFFGYQNENLFYLDPHNTQQSSLMMDDLEFY--RSCHGHKFNKLHI 327
S+GI GGKP +S Y G Q + FYLDPH+ QQ + + + S H + + +
Sbjct: 341 QSLGILGGKPGASTYIVGVQEDKGFYLDPHDVQQVVTVKKENQDVDTSSYHCNTLRYVPL 400
Query: 328 SETDPSMLLGMLISGKNEWDQF 349
DPS+ LG K+++D F
Sbjct: 401 ESLDPSLALGFYCQHKDDFDDF 422
>7296129 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 411
Score = 110 bits (275), Expect = 4e-24
Identities = 91/350 (26%), Positives = 152/350 (43%), Gaps = 59/350 (16%)
Query: 12 GIVEDVDRDGT-VFILGKEYAPLNNKSRTDVETDDSALESLINIVSLNPGLLSDVHSRVF 70
G ED+ R T V++LGK+Y +A++ L I D+ SR++
Sbjct: 38 GEPEDIPRRNTDVWVLGKKY---------------NAIQELELI-------RRDIQSRLW 75
Query: 71 FTYRTQFTPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGWGCMIRTGQAL 130
TYR F+P+ E LT +D GWGCM+R GQ +
Sbjct: 76 CTYRHGFSPLG-----------------------EVQLT------TDKGWGCMLRCGQMV 106
Query: 131 LANAIQRVKLAREFRINASRIDDNELNLIRWFQDDVKYPLSLHNFVKAEEKISGMKPGQW 190
LA A+ + L R++ D L ++ F+D S+H + E G+W
Sbjct: 107 LAQALIDLHLGRDWFWTPDCRDATYLKIVNRFEDVRNSFYSIHQIAQMGES-QNKAVGEW 165
Query: 191 FGPSATARSIKTLIEGFPLCGIKNCIISTQSADIYEDEVTRIFHKDRDANLLLLFAVRLG 250
GP+ A+ +K L+ F ++ S + +D LLL+ +RLG
Sbjct: 166 LGPNTVAQILKKLVR-FDDWSSLAIHVAMDSTVVLDDVYASCREGGSWKPLLLIIPLRLG 224
Query: 251 VDKINSLYWKDIFKILSSPYSVGIAGGKPSSSLYFFGYQNENLFYLDPHNTQQSSLMMD- 309
+ IN LY + + L S G+ GG+P+ +LYF GY ++ + YLDPH TQ++ +
Sbjct: 225 ITDINPLYVPALKRCLELDSSCGMIGGRPNQALYFLGYVDDEVLYLDPHTTQRTGAVAQK 284
Query: 310 ----DLEFYRSCHGHKFNKLHISETDPSMLLGMLISGKNEWDQFHTYFRD 355
+ ++ + H +L+ S DPS+ + L + ++ T ++
Sbjct: 285 TAAAEQDYDETYHQKHAARLNFSAMDPSLAVCFLCKTSDSFESLLTKLKE 334
>Hs16418417 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 398
Score = 110 bits (274), Expect = 6e-24
Identities = 98/368 (26%), Positives = 157/368 (42%), Gaps = 94/368 (25%)
Query: 18 DRDGTVFILGKEYAPLNNKSRTDVETDDSALESLINIVSLNPGLLSDVHSRVFFTYRTQF 77
D D V+ILGK++ KS+ LLSD+ +R++FTYR +F
Sbjct: 24 DTDELVWILGKQHLLKTEKSK----------------------LLSDISARLWFTYRRKF 61
Query: 78 TPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGWGCMIRTGQALLANAIQR 137
+PI GPS SD GWGCM+R GQ +LA A+
Sbjct: 62 SPI--GGTGPS---------------------------SDAGWGCMLRCGQMMLAQALIC 92
Query: 138 VKLAREFRINASRIDDNELN-LIRWFQDDVKYPLSLHNFVKAEEKISGMKPGQWFGPSAT 196
L R++ + E +++ F D S+H + G G+WFGP+
Sbjct: 93 RHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCYSIHQMAQMGVG-EGKSIGEWFGPNTV 151
Query: 197 ARSIK--TLIEGFPLCGI--------------KNCIISTQSADIYEDEVTRIFHKDRDAN 240
A+ +K L + + + K C + SAD D +
Sbjct: 152 AQVLKKLALFDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLSADTAGDRPPDSLTASNQSK 211
Query: 241 -----------LLLLFAVRLGVDKINSLY---WKDIFKILSSPYSVGIAGGKPSSSLYFF 286
LLL+ +RLG+++IN +Y +K+ FK+ P S+G GGKP+++ YF
Sbjct: 212 GTSAYCTAWKPLLLIVPLRLGINQINPVYVDAFKECFKM---PQSLGALGGKPNNAYYFI 268
Query: 287 GYQNENLFYLDPHNTQ-----QSSLMMDDLEFYRSCHGHKFNKLHISETDPSMLLGMLIS 341
G+ + L +LDPH TQ + + ++D F+ + N L++ DPS+ LG
Sbjct: 269 GFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNL---DPSVALGFFCK 325
Query: 342 GKNEWDQF 349
+ ++D +
Sbjct: 326 EEKDFDNW 333
>Hs18378733 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 393
Score = 107 bits (267), Expect = 4e-23
Identities = 95/328 (28%), Positives = 138/328 (41%), Gaps = 75/328 (22%)
Query: 61 LLSDVHSRVFFTYRTQFTPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGW 120
+LSDV SR++FTYR F I GP+ SD GW
Sbjct: 42 ILSDVASRLWFTYRKNFPAI--GGTGPT---------------------------SDTGW 72
Query: 121 GCMIRTGQALLANAIQRVKLAREFR-INASRIDDNELNLIRWFQDDVKYPLSLHNFVKAE 179
GCM+R GQ + A A+ L R++R R D+ +++ F D S+H +
Sbjct: 73 GCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMG 132
Query: 180 EKISGMKPGQWFGPSATARSIKTLIEGFPLCGIKNCIISTQSADIYEDEVTRIFH----- 234
G GQW+GP+ A+ +K L F I+ + + E E+ R+
Sbjct: 133 VG-EGKSIGQWYGPNTVAQVLKKLAV-FDTWSSLAVHIAMDNTVVME-EIRRLCRTSVPC 189
Query: 235 ---------KDRDAN-----------------LLLLFAVRLGVDKINSLYWKDIFKILSS 268
DR N L+LL +RLG+ IN Y + +
Sbjct: 190 AGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMM 249
Query: 269 PYSVGIAGGKPSSSLYFFGYQNENLFYLDPHNTQQS-----SLMMDDLEFYRSCHGHKFN 323
P S+G+ GGKP+S+ YF GY E L YLDPH TQ + + D F+ C H
Sbjct: 250 PQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFH--CQ-HPPC 306
Query: 324 KLHISETDPSMLLGMLISGK---NEWDQ 348
++ I+E DPS+ +G + N+W Q
Sbjct: 307 RMSIAELDPSIAVGFFCKTEDDFNDWCQ 334
>CE24740 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 521
Score = 105 bits (263), Expect = 1e-22
Identities = 92/372 (24%), Positives = 150/372 (39%), Gaps = 78/372 (20%)
Query: 20 DGTVFILGKEYAPLNNKSRTDVETDDSALESLINIVSLNPGLLSDVHSRVFFTYRTQFTP 79
+ V LG+ Y+ D+S L S SD +SR++ TYRT F
Sbjct: 145 ENDVVFLGRRYS---------TSVDESGLRSGFE------NFCSDYYSRLWITYRTDFP- 188
Query: 80 IRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGWGCMIRTGQALLANAIQRVK 139
AL D D+ +D GWGCMIRT Q ++A AI +
Sbjct: 189 ---------------------------ALLDTDTT-TDCGWGCMIRTTQMMVAQAIMVNR 220
Query: 140 LAREFRIN-------ASRIDDNELN--------LIRWFQDDVKYPLSLHNFVKAEEKISG 184
R++R A+ D+++ + +++ F+D PL +H V G
Sbjct: 221 FGRDWRFTRRKRSHVAAHGDEDDFDREKIQEWMILKLFEDKPTAPLGIHKMVGIAAMGKG 280
Query: 185 MKP-GQWFGPSATARSIKTLI--EGFPLCGIKNCIISTQSADIYEDEVTRIFHKDRDANL 241
K G W+ PS +K + PL G ++S D + K+ L
Sbjct: 281 KKAVGSWYSPSEAVFIMKKALTESSSPLTGNTAMLLSIDGRVHIRD--IEVETKNWMKKL 338
Query: 242 LLLFAVRLGVDKINSLYWKDIFKILSSPYSVGIAGGKPSSSLYFFGYQNENLFYLDPHNT 301
+L+ VRLG ++N +Y + ++ + +GI GG+P S +F GY + + YLDPH
Sbjct: 339 ILVIVVRLGAAELNPIYVPHLMRLFAMESCLGITGGRPDHSSWFVGYYGDQIIYLDPHVA 398
Query: 302 QQ---------SSLMMDDLEFYRSC-----HGHKFNKLHISETDPSMLLGMLISGKNEWD 347
+ ++++ D + + C H +K+H + DPS L + ++D
Sbjct: 399 HEYIPIDINPNTNVVDSDSKKAKKCPEKSYHCRLLSKMHFFDMDPSCALCFQFESREQFD 458
Query: 348 QFHTYFRDSQII 359
SQ I
Sbjct: 459 NDMRQLNLSQFI 470
>Hs21361868 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 330
Score = 104 bits (260), Expect = 2e-22
Identities = 72/268 (26%), Positives = 129/268 (47%), Gaps = 18/268 (6%)
Query: 158 LIRWFQDDVKYPLSLHNFVKAEEKISGMKPGQWFGPSATARSIKTLIEGFPLCG--IKNC 215
++ WF D + P LH V+ + SG K G W+GPS A ++ +E C +
Sbjct: 71 IVSWFADHPRAPFGLHRLVELGQS-SGKKAGDWYGPSLVAHILRKAVES---CSDVTRLV 126
Query: 216 IISTQSADIYEDEVTRIFHKDRDA----NLLLLFAVRLGVDKINSLYWKDIFKILSSPYS 271
+ +Q +Y+ +V R+ + ++++L VRLG + +N +Y + ++L
Sbjct: 127 VYVSQDCTVYKADVARLVARPDPTAEWKSVVILVPVRLGGETLNPVYVPCVKELLRCELC 186
Query: 272 VGIAGGKPSSSLYFFGYQNENLFYLDPHNTQQSSLMMDDLEF-YRSCHGHKFNKLHISET 330
+GI GGKP SLYF GYQ++ L YLDPH Q ++ + +F S H K+ ++T
Sbjct: 187 LGIMGGKPRHSLYFIGYQDDFLLYLDPHYC-QPTVDVSQADFPLESFHCTSPRKMAFAKT 245
Query: 331 DPSMLLGMLISGKNEWDQFHTYFRDSQIIQFLTTRPENDLYQSANMSVGSDSIHSLESDI 390
DPS +G + E+ S++ + L++ + Y ++ G HSL+ D+
Sbjct: 246 DPSCTVGFYAGDRKEFGTL-----CSELTRVLSSSSATERYPMFTLAEGHAQDHSLD-DL 299
Query: 391 QDTGEYVDVGTLVSGKQMSSPQPKDEEF 418
+ +G+ + + +P E+F
Sbjct: 300 CSQLAQPTLRLPRTGRLLRAKRPSSEDF 327
>Hs14249578 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 446
Score = 96.3 bits (238), Expect = 8e-20
Identities = 90/342 (26%), Positives = 136/342 (39%), Gaps = 106/342 (30%)
Query: 64 DVHSRVFFTYRTQFTPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGWGCM 123
D SR++ TYR +F I + ALT +D GWGC
Sbjct: 82 DFISRIWLTYREEFPQIEGS-----------------------ALT------TDCGWGCT 112
Query: 124 IRTGQALLANAI--------------------------------------QRVKLAREFR 145
+RTGQ LLA + + REF+
Sbjct: 113 LRTGQMLLAQGLILHFLGRAWTWPDALNIENSDSESWTSHTVKKFTASFEASLSGEREFK 172
Query: 146 INASRI--------DDNELN-------LIRWFQDDVKYPLSLHNFVKAEEKISGMKPGQW 190
+ DD+E+ +I WF D LH ++ +K SG K G W
Sbjct: 173 TPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLALFGLHQLIEYGKK-SGKKAGDW 231
Query: 191 FGPSATARSIKTLIE--------GFPLCGIKNC------IISTQSADIYEDEVTRIFHKD 236
+GP+ A ++ +E G + ++C +I QSA + D
Sbjct: 232 YGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNAD------ 285
Query: 237 RDANLLLLFAVRLGVDKINSLYWKDIFKILSSPYSVGIAGGKPSSSLYFFGYQNENLFYL 296
D +++L VRLG ++ N+ Y + + ILS Y VGI GGKP S YF G+Q+++L Y+
Sbjct: 286 -DKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYM 344
Query: 297 DPHNTQQSSLMMDDLEF-YRSCHGHKFNKLHISETDPSMLLG 337
DPH QS + + +F + H K+ + DPS +G
Sbjct: 345 DPHYC-QSFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIG 385
>At2g44140 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 451
Score = 89.4 bits (220), Expect = 1e-17
Identities = 81/317 (25%), Positives = 117/317 (36%), Gaps = 86/317 (27%)
Query: 64 DVHSRVFFTYRTQFTPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGWGCM 123
D S++ TYR F P FRD ++ SD+ WGCM
Sbjct: 141 DFSSKILMTYRKGFEP----------------FRDT-------------TYTSDVNWGCM 171
Query: 124 IRTGQALLANAIQRVKLAREFRINASRIDDNELNLIRWFQDDVKYPLSLHNFVKAEEKIS 183
IR+ Q L A ++ L + F D S+HN + A
Sbjct: 172 IRSSQMLFAQLPEQ----------------EYLETLEPFGDSEPSAFSIHNLIIAGASY- 214
Query: 184 GMKPGQWFGPSATARSIKTLI-----------------------------EGFPLCGIKN 214
G+ G W GP A R+ ++L G P+ I++
Sbjct: 215 GLAAGSWVGPYAICRAWESLACKKRKQTDSKNQTLPMAVHIVSGSEDGERGGAPILCIED 274
Query: 215 CIISTQSADIYEDEVTRIFHKDRDANLLLLFAVRLGVDKINSLYWKDIFKILSSPYSVGI 274
S + E T I +LL + LG+D +N Y + + P SVGI
Sbjct: 275 ATKSCLEFSKGQSEWTPI---------ILLVPLVLGLDSVNPRYIPSLVATFTFPQSVGI 325
Query: 275 AGGKPSSSLYFFGYQNENLFYLDPHNTQQSSLMMDDLEFY--RSCHGHKFNKLHISETDP 332
GGKP +S Y G Q + FYLDPH QQ + + S H + + + DP
Sbjct: 326 LGGKPGASTYIVGVQEDKGFYLDPHEVQQVVTVNKETPDVDTSSYHCNVLRYVPLESLDP 385
Query: 333 SMLLGMLISGKNEWDQF 349
S+ LG K+++D F
Sbjct: 386 SLALGFYCRDKDDFDDF 402
>CE23129 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 493
Score = 60.5 bits (145), Expect = 5e-09
Identities = 56/209 (26%), Positives = 81/209 (37%), Gaps = 48/209 (22%)
Query: 241 LLLLFAVRLGVDKINSLYWKDIFKILSSPYSVGIAGGKPSSSLYFFGYQNENLFYLDPH- 299
LLL+ +RLG+ IN Y I + P VGI GG+P+ +LYF G LFYLDPH
Sbjct: 269 LLLMIPLRLGLTTINPCYLSAIQEFFKIPQCVGIIGGRPNHALYFVGMSGSKLFYLDPHY 328
Query: 300 ------------NTQQSSLMMDDLEFYR---------------------SCHGHKFNKLH 326
+ S+ DD+ F + H +
Sbjct: 329 CRPKTESTAKMYAEKDSTATTDDVGFSHLEELVPLPSQTADVYTKMDDSTYHCQMMLWIE 388
Query: 327 ISETDPSMLLGMLISGKNEWDQF-----HTYFRDSQ--IIQFLTTRPEN----DLYQSAN 375
DPS+ L M ++E++ T SQ + +FL RP+ + Y +
Sbjct: 389 YENVDPSLALAMFCETRDEFENLCETLQKTTLPASQPPMFEFLQRRPKYLPKFEPYTGVS 448
Query: 376 MSVGS---DSIHSLESDIQDTGEYVDVGT 401
M + D I + I D E +DV T
Sbjct: 449 MKIEMKEFDDIGAANVKIDDDFEVLDVHT 477
Score = 55.1 bits (131), Expect = 2e-07
Identities = 38/139 (27%), Positives = 58/139 (41%), Gaps = 30/139 (21%)
Query: 65 VHSRVFFTYRTQFTPIRRNENGPSPINFTLFFRDNPINTLENALTDPDSFYSDIGWGCMI 124
V SR +FTYR F+PI GPS +D GWGCM+
Sbjct: 57 VTSRFWFTYRRDFSPI--GGTGPS---------------------------TDQGWGCML 87
Query: 125 RTGQALLANAIQRVKLAREFRINASRIDDNELNLIRWFQDDVKYPLSLHNFVKAEEKISG 184
R Q LL + R + R F + + + +++ F D+ S+H + G
Sbjct: 88 RCAQMLLGEVLLRRHIGRHFEWDIEKTSEIYEKILQMFFDEKDALYSIHQIAQM-GVTEG 146
Query: 185 MKPGQWFGPSATARSIKTL 203
+ +WFGP+ A+ +K L
Sbjct: 147 KEVSKWFGPNTAAQVMKKL 165
>HsM14249640 [ZU] KOG2674 Cysteine protease required for autophagy - Apg4p/Aut2p
Length = 141
Score = 55.5 bits (132), Expect = 2e-07
Identities = 39/144 (27%), Positives = 69/144 (47%), Gaps = 8/144 (5%)
Query: 276 GGKPSSSLYFFGYQNENLFYLDPHNTQQSSLMMDDLEF-YRSCHGHKFNKLHISETDPSM 334
GGKP SLYF GYQ++ L YLDPH Q ++ + +F S H K+ ++ DPS
Sbjct: 2 GGKPRHSLYFIGYQDDFLLYLDPHYC-QPTVDVSQADFPLESFHCTSPRKMAFAKMDPSC 60
Query: 335 LLGMLISGKNEWDQFHTYFRDSQIIQFLTTRPENDLYQSANMSVGSDSIHSLESDIQDTG 394
+G + E++ S++ + L++ + Y ++ G HSL+ D+
Sbjct: 61 TVGFYAGDRKEFETL-----CSELTRVLSSSSATERYPMFTLAEGHAQDHSLD-DLCSQL 114
Query: 395 EYVDVGTLVSGKQMSSPQPKDEEF 418
+ +G+ + + +P E+F
Sbjct: 115 AQPTLRLPRTGRLLRAKRPSSEDF 138
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,041,832
Number of Sequences: 60738
Number of extensions: 1228487
Number of successful extensions: 2922
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2874
Number of HSP's gapped (non-prelim): 23
length of query: 450
length of database: 30,389,216
effective HSP length: 110
effective length of query: 340
effective length of database: 23,708,036
effective search space: 8060732240
effective search space used: 8060732240
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)