ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4845 good J KOG3409 Translation, ribosomal structure and biogenesis Exosomal 3'-5' exoribonuclease complex, subunit ski4 (Csl4)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4845 1661012 1660122 -297
(297 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL232w [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subun... 296 3e-80
SPCC1840.11 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex s... 125 6e-29
At5g38890_1 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex s... 99 6e-21
Hs7705612 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex sub... 94 2e-19
7297825 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subun... 76 7e-14
>YNL232w [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subunit ski4
(Csl4)
Length = 292
Score = 296 bits (757), Expect = 3e-80
Identities = 153/289 (52%), Positives = 194/289 (66%), Gaps = 6/289 (2%)
Query: 12 FPQTVIPGDLICPIFDVDDDNKQEVVIRFLTGTGCKQENFRVNGKNVDIIVATLKGSVSV 71
FP+ PG LICP + ++ + ++++ ++ G G K + NG+ ++ I ATL G+V
Sbjct: 7 FPEIAYPGKLICPQYGTENKDGEDIIFNYVPGPGTKLIQYEHNGRTLEAITATLVGTVRC 66
Query: 72 EEVPAXXXXXXXXXXSENTTFVESHETTGGDASSGNSRVIKSFKVSV---SNPSKFASKY 128
EE ++T +S + + D + R +K+ VSV + + +KY
Sbjct: 67 EEEKKTDQEEEREGTDQSTEEEKSVDASPNDVTR---RTVKNILVSVLPGTEKGRKTNKY 123
Query: 129 VDDQFVNNLPQEGDIVLARVTRISLQRITVEILAVENSPLPIDSGVGSNXXXXXXXXXXX 188
++ F NNLP+EGDIVL RVTR+SLQR VEILAVE+ P PIDSG+GSN
Sbjct: 124 ANNDFANNLPKEGDIVLTRVTRLSLQRANVEILAVEDKPSPIDSGIGSNGSGIVAAGGGS 183
Query: 189 XXXXFSVSQVSADLGETFRGIIRSQDVRATDRDRVKVMESFKPGDIIRAQVLSLGDGTHY 248
FSVSQ S+DLGETFRGIIRSQDVR+TDRDRVKV+E FKPGDI+RAQVLSLGDGT+Y
Sbjct: 184 GAATFSVSQASSDLGETFRGIIRSQDVRSTDRDRVKVIECFKPGDIVRAQVLSLGDGTNY 243
Query: 249 YLTTARNDLGVVFAKSSNGAGEQMYAIDWQTMISPKTGVAEKRKCAKPF 297
YLTTARNDLGVVFA+++NGAG MYA DWQ M SP TG EKRKCAKPF
Sbjct: 244 YLTTARNDLGVVFARAANGAGGLMYATDWQMMTSPVTGATEKRKCAKPF 292
>SPCC1840.11 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subunit ski4
(Csl4)
Length = 181
Score = 125 bits (315), Expect = 6e-29
Identities = 79/199 (39%), Positives = 112/199 (55%), Gaps = 41/199 (20%)
Query: 101 GDASSGNSRVIKSFKVSVSNPSKFASKY---VDDQFVNNLPQEGDIVLARVTRISLQRIT 157
G+ + I S + + +P K + Y V++ V LP G IVLARV+RI+ ++ T
Sbjct: 17 GEGTVKRGDYIISTRTGIFDPEKNSVTYPRKVEETAV--LPNVGSIVLARVSRINARQAT 74
Query: 158 VEILAVENSPLPIDSGVGSNXXXXXXXXXXXXXXXFSVSQVSADLGETFRGIIRSQDVRA 217
V I V++ + F+G+I QD+RA
Sbjct: 75 VNISVVDD----------------------------------VCTKDEFQGVIHVQDIRA 100
Query: 218 TDRDRVKVMESFKPGDIIRAQVLSLGDGTHYYLTTARNDLGVVFAKSSNGAGEQMYAIDW 277
T++++VKV SF+PGDI+RA V+SLGDG+ Y+LTTARNDLGV+FA+SS GEQM+ +D+
Sbjct: 101 TEKNKVKVQNSFRPGDIVRALVISLGDGSSYFLTTARNDLGVIFAQSSE-TGEQMFPVDY 159
Query: 278 QTMISPKTGVAEKRKCAKP 296
Q M K+G E RKCAKP
Sbjct: 160 QHM-QTKSGYTEMRKCAKP 177
>At5g38890_1 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subunit ski4
(Csl4)
Length = 206
Score = 99.4 bits (246), Expect = 6e-21
Identities = 63/159 (39%), Positives = 86/159 (53%), Gaps = 37/159 (23%)
Query: 137 LPQEGDIVLARVTRISLQRITVEILAVENSPLPIDSGVGSNXXXXXXXXXXXXXXXFSVS 196
+P+ G +V+ARVT++ + V+IL V
Sbjct: 66 IPETGSVVIARVTKVMTKMAAVDILCVG-------------------------------- 93
Query: 197 QVSADLGETFRGIIRSQDVRATDRDRVKVMESFKPGDIIRAQVLSLGDGTHYYLTTARND 256
S + E F G+IR QDVRAT+ D+V + +SF GDI+RA +LSLGD YYL+TA+N+
Sbjct: 94 --SKAVRENFAGVIRQQDVRATEIDKVDMHQSFHAGDIVRA-MLSLGDARAYYLSTAKNE 150
Query: 257 LGVVFAKSSNGAGEQMYAIDWQTMISPKTGVAEKRKCAK 295
LGVV A+S+ AGE M I W M P +G E+RK AK
Sbjct: 151 LGVVSAESA--AGETMVPISWTEMQCPLSGQTEQRKVAK 187
>Hs7705612 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subunit ski4
(Csl4)
Length = 195
Score = 94.4 bits (233), Expect = 2e-19
Identities = 51/98 (52%), Positives = 68/98 (69%), Gaps = 3/98 (3%)
Query: 199 SADLGETFRGIIRSQDVRATDRDRVKVMESFKPGDIIRAQVLSLGDG-THYYLTTARNDL 257
S L +FRG IR +DVRAT++D+V++ +SF+PGDI+ A+V+SLGD ++Y LTTA N+L
Sbjct: 92 SMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENEL 151
Query: 258 GVVFAKSSNGAGEQMYAIDWQTMISPKTGVAEKRKCAK 295
GVV A S +G QM I W M PKT E RK A+
Sbjct: 152 GVVVAHSESGI--QMVPISWCEMQCPKTHTKEFRKVAR 187
>7297825 [J] KOG3409 Exosomal 3'-5' exoribonuclease complex subunit ski4
(Csl4)
Length = 204
Score = 75.9 bits (185), Expect = 7e-14
Identities = 40/96 (41%), Positives = 64/96 (66%), Gaps = 3/96 (3%)
Query: 202 LGETFRGIIRSQDVRATDRDRVKVMESFKPGDIIRAQVLSLGDGTHYYLTTARNDLGVVF 261
L ++RG++R +DVR T++DRV + +SF+PGD+I A+V++ + + + LTTA N+LGVV
Sbjct: 99 LESSYRGLLRKEDVRETEKDRVDIYKSFRPGDVILARVINQLEQS-FLLTTAENELGVVI 157
Query: 262 AKSSNGAGEQ--MYAIDWQTMISPKTGVAEKRKCAK 295
A +S+ + M + W M P+T + E RK AK
Sbjct: 158 AYASDYRKTRVPMVPVGWSEMQCPQTTIKEPRKVAK 193
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,740,743
Number of Sequences: 60738
Number of extensions: 594278
Number of successful extensions: 1135
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1121
Number of HSP's gapped (non-prelim): 7
length of query: 297
length of database: 30,389,216
effective HSP length: 106
effective length of query: 191
effective length of database: 23,950,988
effective search space: 4574638708
effective search space used: 4574638708
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)