ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV4966 good G KOG0471 Carbohydrate transport and metabolism Alpha-amylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV4966 1705967 1706488 174
(174 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL157c [G] KOG0471 Alpha-amylase 196 2e-50
YJL221c [G] KOG0471 Alpha-amylase 196 2e-50
YIL172c [G] KOG0471 Alpha-amylase 196 2e-50
YGR287c [G] KOG0471 Alpha-amylase 193 9e-50
YGR292w [G] KOG0471 Alpha-amylase 180 8e-46
YJL216c [G] KOG0471 Alpha-amylase 180 1e-45
YBR299w [G] KOG0471 Alpha-amylase 180 1e-45
SPBC1683.07 [G] KOG0471 Alpha-amylase 83 2e-16
7304044 [G] KOG0471 Alpha-amylase 54 8e-08
7304045 [G] KOG0471 Alpha-amylase 49 3e-06
7304046 [G] KOG0471 Alpha-amylase 49 5e-06
>YOL157c [G] KOG0471 Alpha-amylase
Length = 589
Score = 196 bits (497), Expect = 2e-50
Identities = 89/171 (52%), Positives = 117/171 (68%), Gaps = 1/171 (0%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTD-GKEPWFPLTETF 60
Y + ++ G +S ++K ++ + LISRDHARTP W+ E P AGF+ +PWF L E+F
Sbjct: 416 YDAIKEEHGENSKEMKRFLEAIALISRDHARTPMQWSREEPNAGFSGPNAKPWFYLNESF 475
Query: 61 QEGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEK 120
+EGINAEDE KDP SV NFWK A+ RK HKD+ YGYDF F+D N+KLF+FTKKY K
Sbjct: 476 REGINAEDESKDPNSVLNFWKEALRFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYDNK 535
Query: 121 VLYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
L+ LNFS + I+F+ +S+ FGNYP +E+D S L+PWEGR Y
Sbjct: 536 TLFAALNFSSDSIDFTIPNNSSSFKLEFGNYPRSEVDASSRTLKPWEGRIY 586
>YJL221c [G] KOG0471 Alpha-amylase
Length = 589
Score = 196 bits (497), Expect = 2e-50
Identities = 89/171 (52%), Positives = 117/171 (68%), Gaps = 1/171 (0%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTD-GKEPWFPLTETF 60
Y + ++ G +S ++K ++ + LISRDHARTP W+ E P AGF+ +PWF L E+F
Sbjct: 416 YDAIKEEHGENSKEMKRFLEAIALISRDHARTPMQWSREEPNAGFSGPNAKPWFYLNESF 475
Query: 61 QEGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEK 120
+EGINAEDE KDP SV NFWK A+ RK HKD+ YGYDF F+D N+KLF+FTKKY K
Sbjct: 476 REGINAEDESKDPNSVLNFWKEALRFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYDNK 535
Query: 121 VLYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
L+ LNFS + I+F+ +S+ FGNYP +E+D S L+PWEGR Y
Sbjct: 536 TLFAALNFSSDSIDFTIPNNSSSFKLEFGNYPRSEVDASSRTLKPWEGRIY 586
>YIL172c [G] KOG0471 Alpha-amylase
Length = 589
Score = 196 bits (497), Expect = 2e-50
Identities = 89/171 (52%), Positives = 117/171 (68%), Gaps = 1/171 (0%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTD-GKEPWFPLTETF 60
Y + ++ G +S ++K ++ + LISRDHARTP W+ E P AGF+ +PWF L E+F
Sbjct: 416 YDAIKEEHGENSKEMKRFLEAIALISRDHARTPMQWSREEPNAGFSGPNAKPWFYLNESF 475
Query: 61 QEGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEK 120
+EGINAEDE KDP SV NFWK A+ RK HKD+ YGYDF F+D N+KLF+FTKKY K
Sbjct: 476 REGINAEDESKDPNSVLNFWKEALRFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYDNK 535
Query: 121 VLYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
L+ LNFS + I+F+ +S+ FGNYP +E+D S L+PWEGR Y
Sbjct: 536 TLFAALNFSSDSIDFTIPNNSSSFKLEFGNYPRSEVDASSRTLKPWEGRIY 586
>YGR287c [G] KOG0471 Alpha-amylase
Length = 589
Score = 193 bits (491), Expect = 9e-50
Identities = 86/171 (50%), Positives = 117/171 (68%), Gaps = 1/171 (0%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTD-GKEPWFPLTETF 60
Y + ++ G +S+++K ++ + LISRDHARTP W+ E P AGF+ +PWF L ++F
Sbjct: 416 YNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQWSREEPNAGFSGPSAKPWFYLNDSF 475
Query: 61 QEGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEK 120
+EGIN EDE+KDP SV NFWK A++ RK HKD+ YGYDF F+D N+KLF+FTKKY K
Sbjct: 476 REGINVEDEIKDPNSVLNFWKEALKFRKAHKDITVYGYDFEFIDLDNKKLFSFTKKYNNK 535
Query: 121 VLYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
L+ LNFS + +F +S+ FGNYP+ E+D S L+PWEGR Y
Sbjct: 536 TLFAALNFSSDATDFKIPNDDSSFKLEFGNYPKKEVDASSRTLKPWEGRIY 586
>YGR292w [G] KOG0471 Alpha-amylase
Length = 584
Score = 180 bits (457), Expect = 8e-46
Identities = 83/171 (48%), Positives = 117/171 (67%), Gaps = 3/171 (1%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTDGK-EPWFPLTETF 60
Y+++ FG +S ++KD+ G+ L+SRDH+RTP PWT + P AGFT +PWF L E+F
Sbjct: 413 YEIIKKSFGKNSKEMKDFFKGIALLSRDHSRTPMPWTKDKPNAGFTGPDVKPWFLLNESF 472
Query: 61 QEGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEK 120
++GIN E E +D SV NFWK A++ RK +K+++ YGYDF F+D ++++F+FTK+Y +K
Sbjct: 473 EQGINVEQESRDDDSVLNFWKRALQARKKYKELMIYGYDFQFIDLDSDQIFSFTKEYEDK 532
Query: 121 VLYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
L+ LNFS EEIEFS S +I GNY + D S L+PWEGR Y
Sbjct: 533 TLFAALNFSGEEIEFSLPREGASLSFILGNYDDT--DVSSRVLKPWEGRIY 581
>YJL216c [G] KOG0471 Alpha-amylase
Length = 581
Score = 180 bits (456), Expect = 1e-45
Identities = 84/170 (49%), Positives = 112/170 (65%), Gaps = 2/170 (1%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTDGKEPWFPLTETFQ 61
Y + +G DS+++K + L LISRDH RTPFPW+++ P AGF+ +PW + E+F+
Sbjct: 411 YAAIKRDYGEDSEKMKKFFEALALISRDHGRTPFPWSADEPSAGFSKDAKPWIDMNESFR 470
Query: 62 EGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEKV 121
+GINAE ELKD SVF FWK A++VRK HKD+L YG++F F+D N+KLF FTK K
Sbjct: 471 DGINAEAELKDKNSVFFFWKKALQVRKEHKDILVYGHNFQFIDLDNDKLFMFTKDTDNKK 530
Query: 122 LYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
++ V NFS + +FS + SY FGNY + D S LQPWEGR Y
Sbjct: 531 MFAVFNFSSDNTDFSVPDNEASYTMFFGNYANSNGD--SRTLQPWEGRLY 578
>YBR299w [G] KOG0471 Alpha-amylase
Length = 584
Score = 180 bits (456), Expect = 1e-45
Identities = 83/171 (48%), Positives = 117/171 (67%), Gaps = 3/171 (1%)
Query: 2 YKMMVDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTDGK-EPWFPLTETF 60
Y+++ FG +S ++KD+ G+ L+SRDH+RTP PWT + P AGFT +PWF L E+F
Sbjct: 413 YEIIKKSFGKNSKEMKDFFKGIALLSRDHSRTPMPWTKDKPNAGFTGPDVKPWFFLNESF 472
Query: 61 QEGINAEDELKDPKSVFNFWKTAIEVRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEK 120
++GIN E E +D SV NFWK A++ RK +K+++ YGYDF F+D ++++F+FTK+Y +K
Sbjct: 473 EQGINVEQESRDDDSVLNFWKRALQARKKYKELMIYGYDFQFIDLDSDQIFSFTKEYEDK 532
Query: 121 VLYVVLNFSKEEIEFSFLEGYNSYDYIFGNYPEAEIDTGSNKLQPWEGRFY 171
L+ LNFS EEIEFS S +I GNY + D S L+PWEGR Y
Sbjct: 533 TLFAALNFSGEEIEFSLPREGASLSFILGNYDDT--DVSSRVLKPWEGRIY 581
>SPBC1683.07 [G] KOG0471 Alpha-amylase
Length = 579
Score = 83.2 bits (204), Expect = 2e-16
Identities = 45/142 (31%), Positives = 76/142 (52%), Gaps = 4/142 (2%)
Query: 27 SRDHARTPFPWTSEAPYAGFTD-GKEPWFPLTETFQEGINAEDELKDPKSVFNFWKTAIE 85
+RD+ RTP W S +P GFT G +PW +T ++E NA +++ DP+S + FW A+E
Sbjct: 435 ARDNGRTPMHWDS-SPNGGFTKAGVKPWMRVTNDYKEW-NAANQVNDPESPYTFWSKALE 492
Query: 86 VRKTHKDVLNYGYDFTFVDHANEKLFAFTKKYGEKVLYVVLNFSKEEIEFSFLEGYNSYD 145
+RK KD + YG F + + + AF ++ L ++LNF+ ++ + SY+
Sbjct: 493 LRKELKDAVVYG-SFELISEEDPSIVAFVRESSTYKLIILLNFTGNKVSYDCPLNLTSYE 551
Query: 146 YIFGNYPEAEIDTGSNKLQPWE 167
+ NY + T L P++
Sbjct: 552 ILLDNYKDFICMTSPVTLNPYQ 573
>7304044 [G] KOG0471 Alpha-amylase
Length = 519
Score = 54.3 bits (129), Expect = 8e-08
Identities = 33/90 (36%), Positives = 45/90 (49%), Gaps = 7/90 (7%)
Query: 6 VDQFGADSDQVKDYVNGLQLISRDHARTPFPWTSEAPYAGFTDGKEPWFPLTETFQEGIN 65
VD +G +S+ N + +RD RTPF WT AGFT+G W PL + IN
Sbjct: 420 VDPWGCNSNP-----NIYEQYTRDPERTPFQWTG-GTNAGFTNGSSTWLPLAADYAT-IN 472
Query: 66 AEDELKDPKSVFNFWKTAIEVRKTHKDVLN 95
E EL D S +K + +RK+ K + N
Sbjct: 473 VEKELSDDHSHLKIYKALVALRKSSKTLQN 502
>7304045 [G] KOG0471 Alpha-amylase
Length = 547
Score = 49.3 bits (116), Expect = 3e-06
Identities = 34/115 (29%), Positives = 54/115 (46%), Gaps = 8/115 (6%)
Query: 27 SRDHARTPFPWTSEAPYAGFTDGKEPWFPLTETFQEGINAEDELKDPKSVFNFWKTAIEV 86
+RD +RTPF WT+ AGF+ + W PL +Q +N E E +S +K +E+
Sbjct: 389 TRDPSRTPFQWTN-GTNAGFSTASKTWLPLAADYQT-LNVETEAAAQRSHLKIYKALVEL 446
Query: 87 RKTHKDVLNYGYDFTFVDHANEKLFAFTKKY--GEKVLYVVLNFSKEEIEFSFLE 139
RK+ + N + V E +F K+Y G + V NF+ + + E
Sbjct: 447 RKSSLPLQNGSTKYGVV---GENVFV-VKRYISGSASIIYVANFASKGVTVDLYE 497
>7304046 [G] KOG0471 Alpha-amylase
Length = 1043
Score = 48.5 bits (114), Expect = 5e-06
Identities = 25/62 (40%), Positives = 37/62 (59%), Gaps = 2/62 (3%)
Query: 27 SRDHARTPFPWTSEAPYAGFTDGKEPWFPLTETFQEGINAEDELKDPKSVFNFWKTAIEV 86
SRD RTPF WT +A AGF++ + W P+ ++E +N E E + P S N +K ++
Sbjct: 444 SRDPERTPFQWT-DAQDAGFSNASKTWLPIAVDYKE-VNVEQERQKPLSHLNVFKQLWQL 501
Query: 87 RK 88
RK
Sbjct: 502 RK 503
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.138 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,612,179
Number of Sequences: 60738
Number of extensions: 512133
Number of successful extensions: 1178
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1154
Number of HSP's gapped (non-prelim): 12
length of query: 174
length of database: 30,389,216
effective HSP length: 99
effective length of query: 75
effective length of database: 24,376,154
effective search space: 1828211550
effective search space used: 1828211550
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)