ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactMIT381 good C KOG4767 Energy production and conversion Cytochrome c oxidase, subunit II, and related proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactMIT381 35706 34939 -256
(256 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMi025 [C] KOG4767 Cytochrome c oxidase subunit II and related p... 426 e-119
SPMi010 [C] KOG4767 Cytochrome c oxidase subunit II and related ... 260 1e-69
AtMi014 [C] KOG4767 Cytochrome c oxidase subunit II and related ... 226 2e-59
DmMi004 [C] KOG4767 Cytochrome c oxidase subunit II and related ... 218 9e-57
HsMi004 [C] KOG4767 Cytochrome c oxidase subunit II and related ... 191 7e-49
CEMi003 [C] KOG4767 Cytochrome c oxidase subunit II and related ... 130 2e-30
>YMi025 [C] KOG4767 Cytochrome c oxidase subunit II and related proteins
Length = 251
Score = 426 bits (1095), Expect = e-119
Identities = 205/243 (84%), Positives = 218/243 (89%)
Query: 14 LNSIIMNDVPTPYGMYFQDSATPNQEGILELHDNIMFYLFIILGLVSWLLFTIVRTYSKN 73
L + IMNDVPTPY YFQDSATPNQEGILELHDNIMFYL +ILGLVSW+L+TIV TYSKN
Sbjct: 9 LTTFIMNDVPTPYACYFQDSATPNQEGILELHDNIMFYLLVILGLVSWMLYTIVMTYSKN 68
Query: 74 PIAYKYIKHGQTIEIIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGLQWYWKY 133
PIAYKYIKHGQTIE+IWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIG QWYWKY
Sbjct: 69 PIAYKYIKHGQTIEVIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGYQWYWKY 128
Query: 134 EYSDFINDNGETVEFESYVIPEDLLEDGQLRLLXXXXXXXXXXXXHIRFVVTAADVIHDF 193
EYSDFIND+GETVEFESYVIP++LLE+GQLRLL HIRFVVTAADVIHDF
Sbjct: 129 EYSDFINDSGETVEFESYVIPDELLEEGQLRLLDTDTSMVVPVDTHIRFVVTAADVIHDF 188
Query: 194 AVPSLGIKIDAAPGRLNQVSALIQREGVFYGQCSEICGQSHSAMPIKIEAVSLPAFLEWL 253
A+PSLGIK+DA PGRLNQVSALIQREGVFYG CSE+CG H+ MPIKIEAVSLP FLEWL
Sbjct: 189 AIPSLGIKVDATPGRLNQVSALIQREGVFYGACSELCGTGHANMPIKIEAVSLPKFLEWL 248
Query: 254 NEQ 256
NEQ
Sbjct: 249 NEQ 251
>SPMi010 [C] KOG4767 Cytochrome c oxidase subunit II and related proteins
Length = 248
Score = 260 bits (665), Expect = 1e-69
Identities = 123/240 (51%), Positives = 166/240 (68%), Gaps = 2/240 (0%)
Query: 18 IMNDVPTPYGMYFQDSATPNQEGILELHDNIMFYL-FIILGLVSWLLFTIVR-TYSKNPI 75
I+ND P+ + +YFQD A+P+ G+ L+D +MFYL FI +G++ + ++ Y+ +PI
Sbjct: 7 ILNDAPSSWALYFQDGASPSYLGVTHLNDYLMFYLTFIFIGVIYAICKAVIEYNYNSHPI 66
Query: 76 AYKYIKHGQTIEIIWTIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGLQWYWKYEY 135
A KY HG +E IWT+ PA+IL+++A PSF LLYL DEV P+MT+KAIG QW+W YE
Sbjct: 67 AAKYTTHGSIVEFIWTLIPALILILVALPSFKLLYLLDEVQKPSMTVKAIGRQWFWSYEL 126
Query: 136 SDFINDNGETVEFESYVIPEDLLEDGQLRLLXXXXXXXXXXXXHIRFVVTAADVIHDFAV 195
+DF+ + E V F+SY++PE+ LE+G LR L IR ++T+ DVIH +AV
Sbjct: 127 NDFVTNENEPVSFDSYMVPEEDLEEGSLRQLEVDNRLVLPIDTRIRLILTSGDVIHSWAV 186
Query: 196 PSLGIKIDAAPGRLNQVSALIQREGVFYGQCSEICGQSHSAMPIKIEAVSLPAFLEWLNE 255
PSLGIK D P RLNQVS I REG+FYGQCSE+CG HS+MPI ++ VSL FL WL E
Sbjct: 187 PSLGIKCDCIPSRLNQVSLSIDREGLFYGQCSELCGVLHSSMPIVVQGVSLEDFLAWLEE 246
>AtMi014 [C] KOG4767 Cytochrome c oxidase subunit II and related proteins
Length = 260
Score = 226 bits (576), Expect = 2e-59
Identities = 119/236 (50%), Positives = 160/236 (67%), Gaps = 4/236 (1%)
Query: 12 YLLNSIIMNDVPTPYGMYFQDSATPNQEGILELHDNIMFYLFIILGLVSWLLFTIVRT-- 69
+L +I D P+ + QD+ATP +GI++LH +I F+L +IL V W+L +
Sbjct: 6 WLFLTISPCDAAEPWQLGSQDAATPIMQGIIDLHHDIFFFLILILVFVLWILVRALWHFH 65
Query: 70 YSKNPIAYKYIKHGQTIEIIWTIFPAVILLIIAFPSFILLYLCDEVI-SPAMTIKAIGLQ 128
Y KN I + + HG TIEI+ TIFP++I + IA PSF LLY DEV+ PA+TIKAIG Q
Sbjct: 66 YKKNAIPQRIV-HGTTIEILRTIFPSIISMFIAIPSFALLYSMDEVVVDPAITIKAIGHQ 124
Query: 129 WYWKYEYSDFINDNGETVEFESYVIPEDLLEDGQLRLLXXXXXXXXXXXXHIRFVVTAAD 188
WY YEYSD+ + + +++ F+SY+IPE+ LE GQ RLL H+R +VT+AD
Sbjct: 125 WYRTYEYSDYNSSDEQSLTFDSYMIPEEDLELGQSRLLEVDNRVVVPAKTHLRIIVTSAD 184
Query: 189 VIHDFAVPSLGIKIDAAPGRLNQVSALIQREGVFYGQCSEICGQSHSAMPIKIEAV 244
V H +AVPS G+K DA PGRLNQ+S L+QREGV+YGQCSEICG +H+ I +EAV
Sbjct: 185 VPHSWAVPSSGVKCDAVPGRLNQISILVQREGVYYGQCSEICGTNHAFTSIVVEAV 240
>DmMi004 [C] KOG4767 Cytochrome c oxidase subunit II and related proteins
Length = 228
Score = 218 bits (554), Expect = 9e-57
Identities = 110/224 (49%), Positives = 147/224 (65%), Gaps = 9/224 (4%)
Query: 31 QDSATPNQEGILELHDNIMFYLFIILGLVSWLLFTIVRTYSKNPIAYKYIKHGQTIEIIW 90
QDSA+P E ++ HD+ + L +I LV +L+F + N +++ HGQ IE+IW
Sbjct: 10 QDSASPLMEQLIFFHDHALLILVMITVLVGYLMFMLFF----NNYVNRFLLHGQLIEMIW 65
Query: 91 TIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGLQWYWKYEYSDFINDNGETVEFES 150
TI PA+ILL IA PS LLYL DE+ P++T+K+IG QWYW YEYSDF N +EF+S
Sbjct: 66 TILPAIILLFIALPSLRLLYLLDEINEPSVTLKSIGHQWYWSYEYSDFNN-----IEFDS 120
Query: 151 YVIPEDLLEDGQLRLLXXXXXXXXXXXXHIRFVVTAADVIHDFAVPSLGIKIDAAPGRLN 210
Y+IP + L RLL IR +VTAADVIH + VP+LG+K+D PGRLN
Sbjct: 121 YMIPTNELMTDGFRLLDVDNRVVLPMNSQIRILVTAADVIHSWTVPALGVKVDGTPGRLN 180
Query: 211 QVSALIQREGVFYGQCSEICGQSHSAMPIKIEAVSLPAFLEWLN 254
Q + I R G+FYGQCSEICG +HS MPI IE+V + F++W++
Sbjct: 181 QTNFFINRPGLFYGQCSEICGANHSFMPIVIESVPVNYFIKWIS 224
>HsMi004 [C] KOG4767 Cytochrome c oxidase subunit II and related proteins
Length = 227
Score = 191 bits (486), Expect = 7e-49
Identities = 99/219 (45%), Positives = 136/219 (61%), Gaps = 9/219 (4%)
Query: 31 QDSATPNQEGILELHDNIMFYLFIILGLVSWLLFTIVRTYSKNPIAYKYIKHGQTIEIIW 90
QD+ +P E ++ HD+ + +F+I LV + LF + T N I Q +E +W
Sbjct: 10 QDATSPIMEELITFHDHALMIIFLICFLVLYALFLTLTTKLTNT----NISDAQEMETVW 65
Query: 91 TIFPAVILLIIAFPSFILLYLCDEVISPAMTIKAIGLQWYWKYEYSDFINDNGETVEFES 150
TI PA+IL++IA PS +LY+ DEV P++TIK+IG QWYW YEY+D+ G + F S
Sbjct: 66 TILPAIILVLIALPSLRILYMTDEVNDPSLTIKSIGHQWYWTYEYTDY----GGLI-FNS 120
Query: 151 YVIPEDLLEDGQLRLLXXXXXXXXXXXXHIRFVVTAADVIHDFAVPSLGIKIDAAPGRLN 210
Y++P LE G LRLL IR ++T+ DV+H +AVP+LG+K DA PGRLN
Sbjct: 121 YMLPPLFLEPGDLRLLDVDNRVVLPIEAPIRMMITSQDVLHSWAVPTLGLKTDAIPGRLN 180
Query: 211 QVSALIQREGVFYGQCSEICGQSHSAMPIKIEAVSLPAF 249
Q + R GV+YGQCSEICG +HS MPI +E + L F
Sbjct: 181 QTTFTATRPGVYYGQCSEICGANHSFMPIVLELIPLKIF 219
>CEMi003 [C] KOG4767 Cytochrome c oxidase subunit II and related proteins
Length = 231
Score = 130 bits (326), Expect = 2e-30
Identities = 73/167 (43%), Positives = 96/167 (56%), Gaps = 6/167 (3%)
Query: 87 EIIWTIFPAVILLIIAFPSFILLYLCDEV-ISPAMTIKAIGLQWYWKYEYSDFINDNGET 145
E++ +IFP +ILL+ PS LLY + + +T+K G QWYW YEYSD
Sbjct: 64 ELLCSIFPTIILLMQMVPSLSLLYYYGLMNLDSNLTVKVTGHQWYWSYEYSDIPG----- 118
Query: 146 VEFESYVIPEDLLEDGQLRLLXXXXXXXXXXXXHIRFVVTAADVIHDFAVPSLGIKIDAA 205
+EF+SY+ D L G+ RLL +IRF +T+ADVIH +A+ SL +K+DA
Sbjct: 119 LEFDSYMKSLDQLSLGEPRLLEVDNRCVIPCDTNIRFCITSADVIHAWALNSLSVKLDAM 178
Query: 206 PGRLNQVSALIQREGVFYGQCSEICGQSHSAMPIKIEAVSLPAFLEW 252
G L+ S GVFYGQCSEICG +HS MPI +E L F W
Sbjct: 179 SGILSTFSYSFPMVGVFYGQCSEICGANHSFMPIALEVTLLDNFKSW 225
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.143 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,784,312
Number of Sequences: 60738
Number of extensions: 571449
Number of successful extensions: 1475
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1460
Number of HSP's gapped (non-prelim): 6
length of query: 256
length of database: 30,389,216
effective HSP length: 104
effective length of query: 152
effective length of database: 24,072,464
effective search space: 3659014528
effective search space used: 3659014528
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)