ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactV0099 good R KOG1337 General function prediction only N-methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactV0099 32524 34197 558
(558 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL039w [R] KOG1337 N-methyltransferase 333 4e-91
YPL208w [R] KOG1337 N-methyltransferase 156 9e-38
SPBC1709.13c [R] KOG1337 N-methyltransferase 102 2e-21
At1g14030 [R] KOG1337 N-methyltransferase 67 5e-11
At1g24610 [R] KOG1337 N-methyltransferase 61 5e-09
YDR198c [R] KOG1337 N-methyltransferase 57 6e-08
Hs8393013 [R] KOG1337 N-methyltransferase 50 7e-06
At3g56570 [R] KOG1337 N-methyltransferase 50 7e-06
>YHL039w [R] KOG1337 N-methyltransferase
Length = 585
Score = 333 bits (854), Expect = 4e-91
Identities = 207/593 (34%), Positives = 326/593 (54%), Gaps = 48/593 (8%)
Query: 1 MVKEAALERLLVHAKKNNCEISDDLEFRIDPVTGVGAYLKHKLHNK--EALIKISQDFIL 58
M+ + L+ L A+ N I + FRI GV A++ K K +ALI++ + ++
Sbjct: 1 MITQTELDNCLQWAQNNGAFIDPKISFRITEDAGVSAFVNEKFSPKPDQALIRVPETLLI 60
Query: 59 TKAEALQYFEIDSCSSSNPNAITQLYLIALQSNQSAK-----WQPYLDVLPSLDDISSPL 113
T +AL F + S N++TQLYL L+ A ++PYLDVLP + P
Sbjct: 61 TSQQALSEFSQAANERSLLNSVTQLYLSKLKFGTDAVHLKSFYKPYLDVLPL--HLPQPY 118
Query: 114 VWQPHELEIIRGSDLYIKTKRKLASLLDEWYEILTELNL-CSEKAKKYYEL--QDRDNIA 170
W E+ + G+D+Y+ + L L+ EW + L++ S + K++ L +++D+
Sbjct: 119 FWSTDEVMNLHGTDVYLTMRDTLNKLVKEWRMLFQALSIEHSSQDKQFLSLFQENKDSAV 178
Query: 171 V---EKCYSVD----------SFAAYLWAHLIFSSRAFPSIIYDNSA----GLEEGFLLP 213
V + C ++ SF AYLW++ IF+SRAFP +I + L EGFL P
Sbjct: 179 VPLEQFCAHINGCKLEDSEWNSFVAYLWSYCIFNSRAFPRVILGRAGTDRTNLNEGFLYP 238
Query: 214 IVDLLNHKSDTKVHWK-SEGSFITFSSEEI-IEAKGELYNNYGDKSNEELLLGYGFAIDS 271
IVDLLNHK+D V W+ +E + + F S+ A+ EL+NNYG+ SNE+ LL YGF S
Sbjct: 239 IVDLLNHKNDVPVRWEMNEQNELCFMSQTTTFSAQDELFNNYGNISNEKCLLNYGFWDSS 298
Query: 272 NPHDATSISLKLDEKTLS-------EASNYGTEINPNNVSGDSVRFDISLKDPLPVPLIK 324
N D + ++LKL +S ++ N+ T+ + ++F + + +PLP L+
Sbjct: 299 NKFDFSRLTLKLPSTLVSGLPVDFNKSGNFVTDDGETTI----LQFSLKISEPLPPVLLA 354
Query: 325 LFGFISKLKSEKNITVRAVLEGLDQLYALLHQKIAIFKA-KTKFESHNIRNESIIKNAKI 383
LF ++SKLKSE+ TVR+VLEG+DQL +++ Q++ +K K K S +IK K+
Sbjct: 355 LFAYLSKLKSEETPTVRSVLEGIDQLTSVVSQRLLFYKNFKIKTSSTQKLRPHVIKLIKL 414
Query: 384 YLTGQRRLFHSSQEELQREMKAIMKIYKP--VSFKSIYKLDPEFXXXXXXXXXXXXYEEL 441
Y +++ +++ E+L K I K +SFK+I+K D F YE+L
Sbjct: 415 YYQDNKKILNATTEKLSVLQKKIYSNNKEFSLSFKTIFKNDKIFANSLLLVFGAINYEDL 474
Query: 442 VKKGLAQHALLLWIVKAHNAKFYKSPHSPSFVSDTFNDVERNITIEKDDVLEYMQFYQAF 501
+ K ALLLWIVK N K S + F+ TF +V +I IEK+DV+E++ FY+ +
Sbjct: 475 ITKDCLNDALLLWIVKLINDK---SNNQGGFIKQTFKEVSDSIVIEKEDVMEFLPFYKKY 531
Query: 502 FPNLSQMFPKVYSVGSWSIKDFIIAGVVMDRIVWLRMASNEYFLFEKQTFDFK 554
FPNLS+ P++YSVG W I+ FI+A +DR+VW+R ++ E K+ +D +
Sbjct: 532 FPNLSERIPEIYSVGDWGIRQFIVADTAIDRLVWIRKSNKEPIFLMKKAYDLQ 584
>YPL208w [R] KOG1337 N-methyltransferase
Length = 583
Score = 156 bits (394), Expect = 9e-38
Identities = 137/553 (24%), Positives = 238/553 (42%), Gaps = 68/553 (12%)
Query: 6 ALERLLVHAKKNNCEISDDLEFRIDPVTGVGAYLKHKLHNKEALIKISQDFILTKAEALQ 65
AL+ LL + ++L+F + G+ + + N IKI + ++++ ++
Sbjct: 5 ALKALLQWGASFGVIVPEELKFLYTDLKGIICVCEKDIDNPS--IKIPPEIVISRNLPMK 62
Query: 66 YFEIDSCSSSNPNAITQLYLIALQSNQ-----------SAKWQPYLDVLPSLDDISSPLV 114
+F + S+ N N +L+ ++ ++ + K++PYLD LPS ++SPLV
Sbjct: 63 FFGLSE-STKNINGWLKLFFAKIKFDRDNDTIVDNVRVNDKFKPYLDALPSR--LNSPLV 119
Query: 115 WQPHELEIIRGSDLYIKTKRKLASLLDEWYEILT-----ELNLCSEKAKKYYELQDRDNI 169
W P EL+ + +++ K + EW+E+++ +L ++ + ++ L +
Sbjct: 120 WNPSELKRLSSTNIGNSIHEKFEGIFKEWFELVSSSDMFDLERVADDVQTFHNLDELTYE 179
Query: 170 AV-EKCYSVD---------SFAAYLWAHLIFSSRAFPSIIYDNSAGLEEGFLLPIVDLLN 219
A+ EK + SF A+LW+HLIF SRAFP + + + LLPIVDLLN
Sbjct: 180 ALYEKILKITELQRPTIWYSFPAFLWSHLIFISRAFPEYVLNRNCPDNSIVLLPIVDLLN 239
Query: 220 HKSDTKVHWKSEGSFITFSSEEIIEAKGELYNNYGDKSNEELLLGYGFAIDSNPHDATSI 279
H +KV W E + + EL NNYG K NEELL GYGF ++ N D+ ++
Sbjct: 240 HDYRSKVKWYPENGWFCYEKIGTASQSRELSNNYGGKGNEELLSGYGFVLEDNIFDSVAL 299
Query: 280 SLKLDEKTLSEA------------SNYGTEINPNN----------------VSGDSVRFD 311
+KL +S S+Y T N ++G + +
Sbjct: 300 KVKLPLDVVSTILETEPSLKLPLLSDYTTYAFENKDCVQQEKKATRSATDYINGVTYFIN 359
Query: 312 ISLKDPLPVPLIKLFGFISKLKSEKNITVRAVLEGLDQLYALLHQKIAIFKAKTKFESHN 371
I + L PL+ LF ++SK + E +RA L+G+ L L K+ +
Sbjct: 360 IQNEQCLE-PLLDLFTYLSKAEEEDLHDLRARLQGIQMLRNALQSKLNSITGPPATDDSY 418
Query: 372 IRNESIIKNAKIYLTGQRRLFHSSQEELQREMKAIMKIYKP--VSFKSIYKLDPEFXXXX 429
+ + A +Y GQ+++ + L++ K ++ K ++ I K DP F
Sbjct: 419 AIDPYRVYCADVYTKGQKQILKEALTRLKKLEKTMLSENKHQLLTMSKILKNDPAFAETE 478
Query: 430 XXXXXXXXYEELVKKGLAQHALLLWIVKAHNAKFYKSPHSPSFVSDTFNDVERNITIEKD 489
E V L+LWI+ Y P +V + + ++ I D
Sbjct: 479 LPSLFSNEDGEEVIFESTYDLLILWILLKTKKNSY--PTKYEWVGQQYTNFKQTAYISDD 536
Query: 490 DVLEYMQFYQAFF 502
F+ A+F
Sbjct: 537 ----AKAFHTAYF 545
>SPBC1709.13c [R] KOG1337 N-methyltransferase
Length = 547
Score = 102 bits (253), Expect = 2e-21
Identities = 78/290 (26%), Positives = 141/290 (47%), Gaps = 39/290 (13%)
Query: 7 LERLLVHAKKNNCEISDDLEF--RIDPVTGVGAYL---KHKLHNKEALIKISQDFILTKA 61
+E+LL A +N C++ +EF D G+Y+ ++ + + LI ++ +T
Sbjct: 1 MEKLLHEALQNGCKLHKSVEFIQSRDDNACFGSYIAVAQNDIAPDQLLISCPFEYAITYN 60
Query: 62 EALQYFEI--DSCSSSNPNAITQLYLIALQSNQS--AKWQPYLDVLPSLDDISSPLVWQP 117
+A + + + S NP+ IT +AL+S + +KW Y++ LP ++PL +
Sbjct: 61 KAKEELKKLNPNFESCNPH-ITLCTFLALESLKGIQSKWYGYIEYLPKT--FNTPLYFNE 117
Query: 118 HELEIIRGSDLYIKTKRKLASLLDEWYEILTELNLCSEKAKKYYELQDRDNIAVEKCYSV 177
++ + ++ Y + +L E+ E L+ L +++
Sbjct: 118 NDNAFLISTNAYSAAQERLHIWKHEYQEALS---LHPSPTERF----------------- 157
Query: 178 DSFAAYLWAHLIFSSRAFPS-IIYDNSAGLEEGFLLPIVDLLNHKSDTKVHWKSE---GS 233
+F Y+W+ +FSSR F S +IY +S LLP++D LNHK + W S+
Sbjct: 158 -TFDLYIWSATVFSSRCFSSNLIYKDSESTP--ILLPLIDSLNHKPKQPILWNSDFQDEK 214
Query: 234 FITFSSEEIIEAKGELYNNYGDKSNEELLLGYGFAIDSNPHDATSISLKL 283
+ S+E++ +L+NNYG K NEELL+GYGF + NP D ++ + +
Sbjct: 215 SVQLISQELVAKGNQLFNNYGPKGNEELLMGYGFCLPDNPFDTVTLKVAI 264
>At1g14030 [R] KOG1337 N-methyltransferase
Length = 482
Score = 67.4 bits (163), Expect = 5e-11
Identities = 72/308 (23%), Positives = 125/308 (40%), Gaps = 37/308 (12%)
Query: 34 GVGAYLKHKLHNKEALIKISQDFILTKAEALQYFEIDSCSSSNPNAITQLYLIALQSNQS 93
G+G + + E +++I + + C P L+LI + +
Sbjct: 76 GLGLVARRDIGRNEVVLEIPKRLWINPETVTASKIGPLCGGLKPWVSVALFLIREKYEEE 135
Query: 94 AKWQPYLDVLPSLDDISSPLVWQPHELEIIRGSDLYIKTKRKLASLLDEWYEILTELNLC 153
+ W+ YLD+LP D S + W EL ++G+ L T + +E+ ++ E+ L
Sbjct: 136 SSWRVYLDMLPQSTD--STVFWSEEELAELKGTQLLSTTLGVKEYVENEFLKLEQEILLP 193
Query: 154 SEKAKKYYELQDRDNIAVEKCYSVDSFAAYLWAHLIFSSRAFPSIIYDNSAGLEEGFLLP 213
++ ++D F +WA I SRAF + N L+P
Sbjct: 194 NKD-------------LFSSRITLDDF---IWAFGILKSRAFSRLRGQNLV------LIP 231
Query: 214 IVDLLNHK-----SDTKVHWKSEGSF-----ITFSSEEIIEAKGELYNNYG-DKSNEELL 262
+ DL+NH D K G F + S ++A ++Y Y +KSN EL
Sbjct: 232 LADLINHNPAIKTEDYAYEIKGAGLFSRDLLFSLKSPVYVKAGEQVYIQYDLNKSNAELA 291
Query: 263 LGYGFAIDSNPHDATSISLKLDEKTLSEASNYGTEINPNNVSGDSVRFDISLKDPLPVPL 322
L YGF ++SNP S +L ++ +I +N G++ FDI LP +
Sbjct: 292 LDYGF-VESNP-KRNSYTLTIEIPESDPFFGDKLDIAESNKMGETGYFDIVDGQTLPAGM 349
Query: 323 IKLFGFIS 330
++ ++
Sbjct: 350 LQYLRLVA 357
>At1g24610 [R] KOG1337 N-methyltransferase
Length = 471
Score = 60.8 bits (146), Expect = 5e-09
Identities = 85/368 (23%), Positives = 159/368 (43%), Gaps = 76/368 (20%)
Query: 67 FEIDSCSSSNPNAITQLY---------------LIALQSNQSAKWQPYLDVLPSLDDISS 111
FE D SSS+ + ++ L L+ ++N + W PY+ LP + +
Sbjct: 81 FESDDSSSSSSSLLSALARRVPEELWAMKLGLRLLQERANADSFWWPYISNLP--ETYTV 138
Query: 112 PLVWQPHELEIIRGSDLYIKTKRKLASLLDEWYEILTELNLCSEKAKKY-YELQDRDNIA 170
P+ + +++ ++ + L + ++ LL+ EI L KA + + QD + A
Sbjct: 139 PIFFPGEDIKNLQYAPLLHQVNKRCRFLLEFEQEIRRTLE--DVKASDHPFSGQDVNASA 196
Query: 171 VEKCYSVDSFAAYLWAHLIFSSRAFPSIIYDNSAGLEEG------FLLPIVDLLNH--KS 222
+ W S+RAF ++ N L+ G +LP++D+ NH K
Sbjct: 197 LG------------WTMSAVSTRAFR--LHGNKK-LQGGSSDDVPMMLPLIDMCNHSFKP 241
Query: 223 DTKVHWKSEGS----FITFSSEEIIEAKGELYNNYGDKSNEELLLGYGFAIDSNPHDATS 278
+ ++ + G+ + +E ++ L NYG SN+ LL YGF I+SNP+D +
Sbjct: 242 NARIIQEQNGADSNTLVKVVAETEVKENDPLLLNYGCLSNDFFLLDYGFVIESNPYD--T 299
Query: 279 ISLKLDEKTLSEASNYGTEINPN---------------NVSGDSVRFDISLKDPLPVP-- 321
I LK DE+ + AS +P N++G+ +++ P PV
Sbjct: 300 IELKYDEQLMDAASMAAGVSSPKFSSPAPWQHQLLSQLNLAGEMPNLKVTIGGPEPVEGR 359
Query: 322 -----LIKLFGFISKLKSEKNITVRAVLE----GLDQLYALLHQKIAI-FKAKTKFESHN 371
I L G + +++ + T++++ G+ A+ IA+ A + F +
Sbjct: 360 LLAALRILLCGELVEVEKHDSDTLKSLSAVAPFGIANEIAVFRTVIALCVIALSHFPTKI 419
Query: 372 IRNESIIK 379
+ +E+IIK
Sbjct: 420 MEDEAIIK 427
>YDR198c [R] KOG1337 N-methyltransferase
Length = 479
Score = 57.4 bits (137), Expect = 6e-08
Identities = 60/237 (25%), Positives = 103/237 (43%), Gaps = 33/237 (13%)
Query: 96 WQPYLDVLPSLDDISS-PLVWQPHELEIIRGSDLYI-----KTKRKLASLLDEWYEILTE 149
W+P+ DV PS++++ S P +W Y+ K +++ L+ E +E ++E
Sbjct: 148 WKPFFDVWPSMEELRSIPAIWNCDPNSRYHSLIEYLPAASRKHMARISGLVREDWETISE 207
Query: 150 LNLCSEKAKKYYELQDRDNIAVEKCYSVDSFAAYLWAHLIFSSRAF----PSIIYDNSAG 205
+ L + Y L N +K S + F+ ++ + I +SR P I D+ +
Sbjct: 208 VVLKWNEI--YGSLSCTKNS--DKFTSDELFSLFVHVYFIINSRCLYAKIPLKIEDSPSN 263
Query: 206 LEEGFLLPIVDLLNHKSDTKVHW--------KSEGSFITFSSEEIIEAKGELYNN----- 252
L+P VD +NH ++ +H +SEG I + I LY+N
Sbjct: 264 FT---LVPYVDFMNHICESDLHCYPQLSPQLRSEGENIIGIGQFTIRCGDHLYDNINEEL 320
Query: 253 ---YGDKSNEELLLGYGFAIDSNPHDATSISLKLDEKTLSEASNYGTEINPNNVSGD 306
YG SN+ LL YGF +D N + IS ++ E + T + ++ GD
Sbjct: 321 FLNYGAHSNDFLLNEYGFVVDGNKWNYLDISDEIIELIDDDKKEVKTFLLEHDYWGD 377
>Hs8393013 [R] KOG1337 N-methyltransferase
Length = 440
Score = 50.4 bits (119), Expect = 7e-06
Identities = 62/268 (23%), Positives = 107/268 (39%), Gaps = 26/268 (9%)
Query: 31 PVTGVGAYLKHKLHNKEALIKISQDFILTKAEALQYFEIDSCSSSNPNAITQLYLIAL-- 88
P TG G + L + +I + + +LT ++ + + P L L
Sbjct: 56 PGTGRGLMSQTSLQEGQMIISLPESCLLTTDTVIRSYLGAYITKWKPPPSPLLALCTFLV 115
Query: 89 ---QSNQSAKWQPYLDVLPSLDDISSPLVWQPHELEIIRGSDLYIKTKRKLASLLDEWYE 145
+ + W+PYL++LP + P+ +P + ++ S L K + + A + + +
Sbjct: 116 SEKHAGHRSLWKPYLEILPKA--YTCPVCLEPEVVNLLPKS-LKAKAEEQRAHVQEFF-- 170
Query: 146 ILTELNLCSEKAKKYYELQDRDNIAVEKCYSVDSFAAYLWAHLIFSSRAF---PSIIYDN 202
+ + LQ AV+ S+ S++A LWA ++RA P
Sbjct: 171 --------ASSRDFFSSLQPLFAEAVD---SIFSYSALLWAWCTVNTRAVYLRPRQRECL 219
Query: 203 SAGLEEGFLLPIVDLLNHKSDTKVH--WKSEGSFITFSSEEIIEAKGELYNNYGDKSNEE 260
SA + L P +DLLNH +V + E + E++ YG N+
Sbjct: 220 SAEPDTCALAPYLDLLNHSPHVQVKAAFNEETHSYEIRTTSRWRKHEEVFICYGPHDNQR 279
Query: 261 LLLGYGFAIDSNPHDATSISLKLDEKTL 288
L L YGF NPH +S ++ K L
Sbjct: 280 LFLEYGFVSVHNPHACVYVSREILVKYL 307
>At3g56570 [R] KOG1337 N-methyltransferase
Length = 531
Score = 50.4 bits (119), Expect = 7e-06
Identities = 49/217 (22%), Positives = 89/217 (40%), Gaps = 25/217 (11%)
Query: 17 NNCEISDDLEFRIDPVTGVGAYLKHKLHNKEALIKISQDFILTKAEALQYFEIDSCSSSN 76
N + S+ L D GV L + + IS+ LT + I+S
Sbjct: 16 NGVDCSEALNLVDDENDGVSVRAFCDLKEGDVVANISKTACLTIKTSGAREMIESADLDG 75
Query: 77 PNAITQLYLIALQSNQSAKWQPYLDVLPSLDDISSPLVWQPHELE-IIRGSDLYIKTKRK 135
++ + + + W YL +LP +D+ PLVW +L+ ++ G++L+ K
Sbjct: 76 SLGLSVALMYERSLGEESPWAGYLQILPIQEDL--PLVWSLEDLDSLLSGTELHKLVKED 133
Query: 136 LASLLDEWYEILTELNLCSEKAKKYYELQDRDNIAVEKCYSVDSFAAYLWAHLIFSSRAF 195
+ ++W E + L + D D+ +++ YL A + +SR+F
Sbjct: 134 HVLIYEDWKENILPL------TSSLPQNVDSDSFGIKE---------YLAAKSLIASRSF 178
Query: 196 PSIIYDNSAGLEEGFLLPIVDLLNHKSDTK-VHWKSE 231
Y S ++P+ DL NHK+ + VH+ E
Sbjct: 179 EIDDYHGSG------MVPLADLFNHKTGAEDVHFTHE 209
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,332,314
Number of Sequences: 60738
Number of extensions: 1372540
Number of successful extensions: 3453
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3427
Number of HSP's gapped (non-prelim): 10
length of query: 558
length of database: 30,389,216
effective HSP length: 112
effective length of query: 446
effective length of database: 23,586,560
effective search space: 10519605760
effective search space used: 10519605760
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)